##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939561_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1838206 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.312817497059633 31.0 31.0 34.0 30.0 34.0 2 31.530661416620337 31.0 31.0 34.0 30.0 34.0 3 31.548319394017863 31.0 31.0 34.0 30.0 34.0 4 35.4023907004982 37.0 35.0 37.0 33.0 37.0 5 35.124079673333675 37.0 35.0 37.0 32.0 37.0 6 35.15229740301142 37.0 35.0 37.0 32.0 37.0 7 35.077385777219746 37.0 35.0 37.0 32.0 37.0 8 35.04111454320136 37.0 35.0 37.0 32.0 37.0 9 36.59811794760761 39.0 35.0 39.0 32.0 39.0 10-11 36.476720781022365 38.0 35.0 39.0 32.0 39.0 12-13 36.404710353464196 38.0 35.0 39.0 32.0 39.0 14-15 37.36145894420974 39.0 36.0 41.0 32.0 41.0 16-17 37.31912065350673 39.0 36.0 40.5 32.0 41.0 18-19 37.33405858755765 39.0 36.0 40.0 32.0 41.0 20-21 37.28860475920544 39.0 36.0 40.0 31.0 41.0 22-23 37.129929942563564 39.0 36.0 40.0 31.0 41.0 24-25 36.9802799577414 39.0 36.0 40.0 31.0 41.0 26-27 36.686300936891726 38.5 35.0 40.0 30.0 41.0 28-29 36.44767833420194 38.0 35.0 40.0 30.0 41.0 30-31 36.13506810444531 38.0 35.0 40.0 30.0 41.0 32-33 35.892172313657994 38.0 34.0 40.0 29.5 41.0 34-35 35.81285693768816 38.0 34.0 40.0 29.0 41.0 36-37 35.65131437934595 38.0 34.0 40.0 29.0 41.0 38-39 35.64088899720706 38.0 34.0 40.0 28.5 41.0 40-41 35.49645877556705 38.0 34.0 40.0 27.5 41.0 42-43 35.24639648657441 38.0 34.0 40.0 27.0 41.0 44-45 34.95260569272432 38.0 33.5 40.0 26.0 41.0 46-47 34.531245953935525 38.0 33.0 40.0 24.5 41.0 48-49 34.135949398489615 38.0 33.0 40.0 23.5 41.0 50-51 33.834703781839465 37.5 33.0 40.0 23.0 41.0 52-53 33.56194436314537 37.0 33.0 40.0 22.5 41.0 54-55 33.2226026353956 37.0 32.0 40.0 20.5 41.0 56-57 31.041272849724134 36.0 28.0 39.0 12.0 40.5 58-59 25.92453212534395 31.5 13.5 37.5 2.0 40.0 60-61 26.645284043246512 31.0 17.5 37.0 2.0 40.0 62-63 29.636006519399892 34.0 27.0 38.0 2.0 40.0 64-65 29.8020763178882 34.5 28.5 38.0 2.0 40.0 66-67 23.216107443888227 26.0 15.0 34.5 2.0 38.0 68-69 16.024595447953057 13.0 7.0 27.5 2.0 34.0 70-71 14.895358572434212 12.0 6.5 24.0 2.0 32.5 72-73 15.827546531781529 12.5 6.5 27.0 2.0 34.0 74-75 16.751180226808096 15.5 6.5 27.5 2.0 34.0 76-77 18.06352470832975 19.5 7.0 29.0 2.0 33.5 78-79 20.946607997145044 24.5 7.5 32.5 2.0 35.0 80-81 24.389403581535475 30.0 16.5 34.0 2.0 36.0 82-83 25.64528975533754 32.0 21.5 35.0 2.0 36.0 84-85 25.697611965144276 33.0 21.0 35.0 2.0 36.0 86-87 25.439431978788015 32.5 20.0 35.0 2.0 35.0 88-89 24.971436552812907 32.0 16.5 35.0 2.0 35.0 90-91 23.551807849609894 31.5 2.0 34.0 2.0 35.0 92-93 20.894550719560268 30.0 2.0 34.0 2.0 35.0 94-95 13.956088708229654 2.0 2.0 31.0 2.0 34.0 96-97 11.625441871041657 2.0 2.0 27.0 2.0 34.0 98-99 11.206438777808364 2.0 2.0 26.5 2.0 34.0 100-101 10.928813473571516 2.0 2.0 27.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3667018521581227 1101 2 0.36606004371955336 1101 3 0.4091470472015324 1101 4 0.26193658914318974 1101 5 0.3960401092518566 1101 6 0.5311980536717442 1101 7 0.3880326471622695 1101 8 0.33605869555032797 1101 9 0.5004569955844076 1101 10-11 0.42605466644848633 1101 12-13 0.4542800845867845 1101 14-15 0.6409383298555369 1101 16-17 0.6517236825044037 1101 18-19 0.5255224421836644 1101 20-21 0.5859075660835771 1101 22-23 0.6543270284033724 1101 24-25 0.5880443748656745 1101 26-27 0.5945540100303219 1101 28-29 0.7287283652376573 1101 30-31 0.5170526462653484 1101 32-33 0.6974166816377831 1101 34-35 0.6254676594073274 1101 36-37 0.3349461963953999 1101 38-39 0.6218389200971757 1101 40-41 0.7471248620940187 1101 42-43 0.7602230217772501 1101 44-45 0.5830983322474026 1101 46-47 0.5220497371396036 1101 48-49 0.5513052946977126 1101 50-51 0.48109105563545285 1101 52-53 0.45529333055972643 1101 54-55 0.10409567600024872 1101 56-57 -2.248113372083182 1101 58-59 -9.5786693263891 1101 60-61 -9.944653984486624 1101 62-63 -4.19389628730357 1101 64-65 -5.704062007463808 1101 66-67 -9.366116127746384 1101 68-69 -4.9058785463178705 1101 70-71 -4.453251853409945 1101 72-73 -4.855191793668741 1101 74-75 -6.811968424287167 1101 76-77 -8.268936433499308 1101 78-79 -10.96364875615085 1101 80-81 -8.128820135323387 1101 82-83 -4.9779794467127765 1101 84-85 -4.2044937549683965 1101 86-87 -4.006066078150784 1101 88-89 -4.1638031127940245 1101 90-91 -9.99376724552929 1101 92-93 -16.018649947918203 1101 94-95 -10.442992442023154 1101 96-97 -8.636498841261346 1101 98-99 -8.380204772843294 1101 100-101 -8.221353190427106 1102 1 0.4088081316517105 1102 2 0.4025301065989737 1102 3 0.45441781490974975 1102 4 0.3511432946719921 1102 5 0.36497695224640836 1102 6 0.4206485527243302 1102 7 0.5116190671388523 1102 8 0.4691337525762833 1102 9 0.7046714856093885 1102 10-11 0.5615519690300417 1102 12-13 0.5275159005106005 1102 14-15 0.7162029423807255 1102 16-17 0.6799494208246415 1102 18-19 0.5846532337884796 1102 20-21 0.7544702705020327 1102 22-23 0.6886307082373051 1102 24-25 0.6181927027076242 1102 26-27 0.6110861074994531 1102 28-29 0.5774279319610471 1102 30-31 0.45690202531599056 1102 32-33 0.5289481153126658 1102 34-35 0.35176235991543336 1102 36-37 0.39601768100141044 1102 38-39 0.51994288234539 1102 40-41 0.5707692132049615 1102 42-43 0.7106412121534404 1102 44-45 0.6446791280763549 1102 46-47 0.7082621271609568 1102 48-49 0.6015593468598723 1102 50-51 0.5620570573009758 1102 52-53 0.2885904569340809 1102 54-55 0.14806661388117703 1102 56-57 -3.0063365759162934 1102 58-59 -9.754296008504069 1102 60-61 -9.944979847117423 1102 62-63 -4.092277836237276 1102 64-65 -5.396979926287802 1102 66-67 -6.8473029918616675 1102 68-69 -4.0193185694903235 1102 70-71 -3.823941113219014 1102 72-73 -4.119421731996221 1102 74-75 -5.067648692701422 1102 76-77 -7.246333635908277 1102 78-79 -9.58495775840775 1102 80-81 -5.757073632312174 1102 82-83 -3.3412040384793116 1102 84-85 -2.6514602152553977 1102 86-87 -2.6299807745554347 1102 88-89 -2.8881269478351186 1102 90-91 -9.133663693626614 1102 92-93 -12.927321514593316 1102 94-95 -9.600101678418389 1102 96-97 -8.454265596031615 1102 98-99 -8.284993746617387 1102 100-101 -8.200341085233822 1103 1 0.3441553606510155 1103 2 0.28495241444236896 1103 3 0.40345729439669853 1103 4 0.25410990776709497 1103 5 0.3626817337868218 1103 6 0.3479365754640398 1103 7 0.17906711280603815 1103 8 0.381467525270601 1103 9 0.43681207947555123 1103 10-11 0.4757308107723759 1103 12-13 0.4122803688488119 1103 14-15 0.611081566336729 1103 16-17 0.5759195706314983 1103 18-19 0.6194597646608955 1103 20-21 0.3944634021410849 1103 22-23 0.424131857699507 1103 24-25 0.5224906166282892 1103 26-27 0.47806139745917164 1103 28-29 0.4543717336976556 1103 30-31 0.4606916064077424 1103 32-33 0.4919024493596069 1103 34-35 0.5681031294436352 1103 36-37 0.4635756698563753 1103 38-39 0.4942217353291056 1103 40-41 0.5817935501796043 1103 42-43 0.49084007343849123 1103 44-45 0.5368678498817943 1103 46-47 0.5630538589391136 1103 48-49 0.49756803854828746 1103 50-51 0.5255706192255545 1103 52-53 0.5526214930312676 1103 54-55 0.5590735328901033 1103 56-57 -2.5095611231418324 1103 58-59 -9.706990172497303 1103 60-61 -9.814015274765541 1103 62-63 -3.9343257937028504 1103 64-65 -5.067687972770301 1103 66-67 -6.29107298083029 1103 68-69 -3.398071492558504 1103 70-71 -3.720246775046121 1103 72-73 -4.080180908237621 1103 74-75 -4.597794126538478 1103 76-77 -6.176689567713854 1103 78-79 -8.687180463776066 1103 80-81 -4.919008583004505 1103 82-83 -2.7689690170033288 1103 84-85 -2.212164543434575 1103 86-87 -2.0791681214533675 1103 88-89 -2.19021240951621 1103 90-91 -5.3943811161629895 1103 92-93 -10.120456365472059 1103 94-95 -8.038157956977367 1103 96-97 -7.4884456977970055 1103 98-99 -7.6031262195114495 1103 100-101 -7.527874071278449 1104 1 0.3141544196255168 1104 2 0.29159313797021724 1104 3 0.2755345614763698 1104 4 0.21927168554426402 1104 5 0.38701373669694306 1104 6 0.26871065893823953 1104 7 0.41217598991198656 1104 8 0.3075744663248088 1104 9 0.37004073796281034 1104 10-11 0.3336024662868624 1104 12-13 0.4021426988689569 1104 14-15 0.5421511067540052 1104 16-17 0.5295096968360014 1104 18-19 0.6089709759657822 1104 20-21 0.5175145622569133 1104 22-23 0.46181138451633785 1104 24-25 0.48767506560088947 1104 26-27 0.4836169795973646 1104 28-29 0.5138798621756564 1104 30-31 0.5021260072467868 1104 32-33 0.6646303000004039 1104 34-35 0.6855755805233983 1104 36-37 0.582865576116113 1104 38-39 0.5203883961855666 1104 40-41 0.4397914352646666 1104 42-43 0.618059657523446 1104 44-45 0.7278441900399599 1104 46-47 0.7378926371188967 1104 48-49 0.9508865661099648 1104 50-51 0.9612906028476047 1104 52-53 0.8200646994081922 1104 54-55 0.5503414770273665 1104 56-57 -2.926073021873819 1104 58-59 -9.788593260701468 1104 60-61 -9.685893741664113 1104 62-63 -3.9567155928953 1104 64-65 -5.619297183336489 1104 66-67 -6.657080405589834 1104 68-69 -3.994733153334078 1104 70-71 -3.5390304414865312 1104 72-73 -3.972891925230993 1104 74-75 -4.7243446255440205 1104 76-77 -6.263185760661905 1104 78-79 -8.533689293352246 1104 80-81 -4.766911748062885 1104 82-83 -2.400464570828735 1104 84-85 -1.7167804671809819 1104 86-87 -1.6020070153815666 1104 88-89 -1.6022894122884779 1104 90-91 -4.52983934431451 1104 92-93 -6.818899048019045 1104 94-95 -7.278941660329673 1104 96-97 -7.1068439083447394 1104 98-99 -7.31183781433549 1104 100-101 -7.283136693722841 1105 1 0.29363526251610494 1105 2 0.27035325087917883 1105 3 0.32756510764754054 1105 4 0.24325673659037506 1105 5 0.28901344405232265 1105 6 0.2544810458959219 1105 7 0.32393530267869863 1105 8 0.16025406306793144 1105 9 0.35378831160553403 1105 10-11 0.39908297300657836 1105 12-13 0.3563556237593133 1105 14-15 0.46741096776633384 1105 16-17 0.4696309961781111 1105 18-19 0.5612930554465763 1105 20-21 0.3836603261334588 1105 22-23 0.47311026820677426 1105 24-25 0.47386404549361316 1105 26-27 0.5091343632433478 1105 28-29 0.5867205894160605 1105 30-31 0.6368005280875266 1105 32-33 0.5324936145896615 1105 34-35 0.5528054491667191 1105 36-37 0.4985013882234526 1105 38-39 0.5738094673389611 1105 40-41 0.46408151739037606 1105 42-43 0.46588364567689666 1105 44-45 0.4507754889375377 1105 46-47 0.6149380238913622 1105 48-49 0.4954037395333941 1105 50-51 0.47687670704390683 1105 52-53 0.7134622987962302 1105 54-55 0.5515788497825227 1105 56-57 1.7791260737061378 1105 58-59 -7.742332490268208 1105 60-61 -9.085977020358104 1105 62-63 -3.510684909662725 1105 64-65 -4.7746172726486655 1105 66-67 -6.132538195308005 1105 68-69 -3.9412170421365325 1105 70-71 -3.720606240147035 1105 72-73 -1.9767000096311822 1105 74-75 -4.021464692259585 1105 76-77 -6.163630214675951 1105 78-79 -8.647420854889113 1105 80-81 -4.669400662758832 1105 82-83 -2.296204716299041 1105 84-85 -1.6873885521368237 1105 86-87 -1.4445852792951506 1105 88-89 -1.7006786629700805 1105 90-91 -4.703977825414853 1105 92-93 -6.726692146421723 1105 94-95 -7.498060938942167 1105 96-97 -7.389696739588812 1105 98-99 -7.4333054734008055 1105 100-101 -7.506223807357857 1106 1 0.20097905329115662 1106 2 0.13907797034681835 1106 3 0.10797921907760255 1106 4 0.11259817872242195 1106 5 0.11144828551891806 1106 6 0.21798635480772077 1106 7 0.20226662444279242 1106 8 0.1600552846884966 1106 9 0.1465389150763059 1106 10-11 0.1950033270971332 1106 12-13 0.21673267066692858 1106 14-15 0.22502367001059298 1106 16-17 0.2453376278325976 1106 18-19 0.28144979474720344 1106 20-21 0.31819852827985784 1106 22-23 0.2521793126026566 1106 24-25 0.32549892121125623 1106 26-27 0.35576868849778975 1106 28-29 0.4409334678085486 1106 30-31 0.42329878699279533 1106 32-33 0.3521078418208745 1106 34-35 0.6092574895503304 1106 36-37 0.42815779724615766 1106 38-39 0.4887302821876034 1106 40-41 0.46129709101381167 1106 42-43 0.3705092695351908 1106 44-45 0.33249114003356794 1106 46-47 0.46482517893441866 1106 48-49 0.5334957932912232 1106 50-51 0.5568202743716739 1106 52-53 0.5494951104807342 1106 54-55 0.4224631179518852 1106 56-57 1.6071700332899042 1106 58-59 -8.005218426137205 1106 60-61 -9.605971576888635 1106 62-63 -3.7764016535301543 1106 64-65 -5.45493016001166 1106 66-67 -7.00798519181766 1106 68-69 -4.046719941242744 1106 70-71 -3.788712809823444 1106 72-73 -4.431358151593605 1106 74-75 -5.01579339218782 1106 76-77 -6.473804507636546 1106 78-79 -8.87865311774141 1106 80-81 -5.232429719061024 1106 82-83 -2.7788299568718493 1106 84-85 -2.2595517298181917 1106 86-87 -2.130510342904234 1106 88-89 -2.194217855950246 1106 90-91 -4.706369791914071 1106 92-93 -6.626904380568384 1106 94-95 -6.868223998375884 1106 96-97 -6.572376109043405 1106 98-99 -6.315566490819908 1106 100-101 -6.461486439469026 1107 1 0.17347033734550976 1107 2 0.018055057640964378 1107 3 0.03926156391304758 1107 4 0.21964112903764743 1107 5 0.08141855028681277 1107 6 0.16342349898214792 1107 7 0.15047163177299439 1107 8 0.19234080508095985 1107 9 0.3107250555571497 1107 10-11 0.26037123584001876 1107 12-13 0.23830133366936224 1107 14-15 0.17858788308891604 1107 16-17 0.16482378851682 1107 18-19 0.22305736220835826 1107 20-21 0.2677887622764459 1107 22-23 0.19292370357432986 1107 24-25 0.2710392839396434 1107 26-27 0.3020191482542032 1107 28-29 0.3467295069098597 1107 30-31 0.4037894442243086 1107 32-33 0.5081612150083927 1107 34-35 0.4042523609998483 1107 36-37 0.4209234328496976 1107 38-39 0.3068725295287891 1107 40-41 0.3013228373733128 1107 42-43 0.3247251711499075 1107 44-45 0.4380418893717817 1107 46-47 0.5424670299785177 1107 48-49 0.14751098208922286 1107 50-51 0.11864741113820543 1107 52-53 0.13813213343487973 1107 54-55 0.166667467377124 1107 56-57 1.5688323052895754 1107 58-59 -8.00761654152441 1107 60-61 -9.595878952158664 1107 62-63 -3.695004175326499 1107 64-65 -5.261773537188059 1107 66-67 -6.658885148820723 1107 68-69 -3.5421033392441625 1107 70-71 -3.697156139340583 1107 72-73 -2.0579189922614542 1107 74-75 -4.457771613979498 1107 76-77 -6.596296957270553 1107 78-79 -6.918269616132909 1107 80-81 -2.144867619567144 1107 82-83 -0.9007593020232996 1107 84-85 -0.6995181611241073 1107 86-87 -0.7309044383367116 1107 88-89 -0.43779046120208776 1107 90-91 -0.12272261546603147 1107 92-93 -3.2299559976158925 1107 94-95 -6.039777120316237 1107 96-97 -6.156944093419457 1107 98-99 -5.840800772330706 1107 100-101 -6.282375569532174 1108 1 -0.0375294427845283 1108 2 -0.09679370323474501 1108 3 -0.09456009013726785 1108 4 -0.039593095292538294 1108 5 0.005113622058651401 1108 6 -0.11569325196800406 1108 7 -0.1733817463719376 1108 8 -0.15988710584029775 1108 9 -0.2669311306413036 1108 10-11 -0.11122470598867551 1108 12-13 -0.08841914878815516 1108 14-15 -0.1964468249551814 1108 16-17 -0.2267727815117624 1108 18-19 -0.24115848727494438 1108 20-21 -0.2306646095864764 1108 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92-93 5.402007043850151 2316 94-95 11.796356042566035 2316 96-97 13.263762997083424 2316 98-99 12.892895473022344 2316 100-101 11.69369680985812 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 13.0 4 42.0 5 112.0 6 295.0 7 690.0 8 1435.0 9 2459.0 10 4061.0 11 6440.0 12 8946.0 13 12321.0 14 15776.0 15 21285.0 16 28985.0 17 37867.0 18 40433.0 19 39200.0 20 40409.0 21 40116.0 22 39516.0 23 34177.0 24 33606.0 25 37657.0 26 52154.0 27 77860.0 28 109371.0 29 144110.0 30 168651.0 31 173136.0 32 163498.0 33 142180.0 34 121750.0 35 103909.0 36 77589.0 37 44077.0 38 13503.0 39 571.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.57655152750466 20.974201152008288 15.278746611500276 22.170500708986772 2 15.74433798691354 22.44430746598494 39.16787217987436 22.64348236722716 3 18.324472324329793 27.14188802868889 31.493692481189512 23.03994716579181 4 12.820597909048278 18.589320239407336 37.68500374821973 30.905078103324655 5 12.68006817873549 38.818082726365006 34.42982563653369 14.072023458365813 6 29.691285960333065 38.38024682761345 17.76634392445678 14.162123287596712 7 26.21138556363409 34.49724051452368 21.966102801374166 17.325271120468066 8 25.65495668257919 35.487970405429294 20.388537544031443 18.46853536796007 9 26.54658944644942 16.069200078772454 20.729287141919894 36.65492333285823 10-11 24.139976694668604 27.489873278620568 28.00273745162403 20.367412575086796 12-13 25.505112049465623 25.207185701711342 28.53475072978763 20.752951519035406 14-15 22.45971660794513 25.831120792102908 27.45213988392421 24.257022716027755 16-17 21.044431364058216 29.20521421429372 27.901007830460788 21.849346591187278 18-19 21.01460530055856 27.15963012780148 31.145507635856223 20.680256935783735 20-21 21.92422938451947 26.487346902360237 31.09830998266788 20.490113730452407 22-23 21.645849200304646 26.62933848329888 30.825263845065827 20.89954847133065 24-25 21.275831238672716 26.75791689231699 31.025094854737407 20.94115701427289 26-27 21.220421488183284 26.943489857746787 30.899939669274467 20.936148984795462 28-29 21.160254146970676 26.820839877507208 31.114833454741696 20.904072520780414 30-31 21.287617302435713 26.805429209394642 30.99306087539391 20.913892612775737 32-33 21.17560175501111 26.87658616065478 30.823565893159753 21.12424619117435 34-35 21.461310428908494 26.873411089851594 30.81801569093824 20.84726279030167 36-37 21.605191401903973 26.69867726207572 30.555796079763997 21.140335256256307 38-39 21.27698621863878 27.038811251742306 30.706734168915546 20.977468360703366 40-41 21.40660040930871 26.81385985193537 30.7601077873501 21.019431951405824 42-43 21.409344796690625 26.975182394287568 30.33423293636421 21.281239872657608 44-45 21.4725107560805 27.054752459690057 30.17805299352497 21.294683790704468 46-47 21.532989922881054 27.264152202155607 29.618266524234016 21.584591350729323 48-49 21.661672183063597 27.43911654143016 29.420117988718502 21.479093286787744 50-51 21.569156569289998 27.568928925614433 29.200093889401117 21.661820615694452 52-53 21.82121504901244 27.717735597159027 28.93240892059922 21.528640433229313 54-55 21.58943267251242 27.608084900547492 28.96626609463089 21.836216332309196 56-57 21.281661696451025 27.67648096146106 29.741453021270004 21.30040432081791 58-59 21.723978466311017 26.460029251476403 30.944665588359594 20.871326693852986 60-61 21.931178219473704 27.25207939586063 29.565658582482474 21.251083802183196 62-63 21.640964234934543 27.659938507839936 29.107585710512453 21.59151154671307 64-65 21.32917242930812 27.536347537529572 29.376833990714772 21.75764604244754 66-67 22.76066749790387 26.21834792437628 30.397312517999502 20.623672059720345 68-69 23.589072844526246 25.182359395033814 31.10548673181578 20.12308102862416 70-71 23.413198316924362 25.341197467938315 31.019015663400552 20.226588551736768 72-73 22.76659082984975 25.585349411819642 31.137980041598873 20.510079716731735 74-75 22.778211225578982 26.100766846159424 30.172170281010274 20.94885164725132 76-77 20.340158689178235 27.272144899483507 30.70407795567914 21.68361845565912 78-79 20.749190553555326 27.435550591158258 29.94657900708269 21.868679848203723 80-81 21.005020557696405 28.061670639766618 28.70914213972593 22.224166662811047 82-83 21.38509336030173 27.97865956987294 28.351741730093714 22.284505339731616 84-85 21.44605553093909 27.728504045124563 28.23209437708237 22.593346046853974 86-87 21.25910810478523 27.776224324039084 28.42721513134058 22.5374524398351 88-89 21.305943902822484 27.713614872786085 28.41021911072934 22.57022211366209 90-91 20.87682717785145 27.799340790914535 28.885167522838444 22.438664508395576 92-93 19.92965214526537 27.90511344110607 29.39224720310457 22.772987210523986 94-95 20.506970370335388 26.628723153941525 30.702937464598108 22.16136901112498 96-97 20.83995029087648 26.379755945498495 30.565029826868866 22.21526393675616 98-99 20.37071711486 26.925624043422474 29.75963426495906 22.94402457675846 100-101 20.366452578494645 27.288233886589037 29.372633471324466 22.972680063591852 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1716.0 1 1325.0 2 916.5 3 960.5 4 1071.0 5 1224.5 6 1556.5 7 2206.0 8 2932.0 9 3446.0 10 3722.5 11 3853.0 12 4100.0 13 4465.5 14 4776.0 15 4988.0 16 5209.5 17 5338.5 18 5459.5 19 5809.0 20 6136.0 21 6587.0 22 7261.0 23 8127.5 24 9250.5 25 10781.0 26 12803.0 27 15179.5 28 17787.5 29 20615.0 30 23829.0 31 27291.5 32 31142.5 33 35229.0 34 39535.0 35 43981.5 36 48187.0 37 51767.5 38 54558.0 39 56589.5 40 58416.5 41 60103.0 42 61880.0 43 64090.0 44 65897.5 45 67241.5 46 68495.5 47 69404.5 48 69982.5 49 69740.0 50 67915.5 51 65225.0 52 61868.0 53 58320.5 54 54703.0 55 50260.0 56 45032.0 57 39497.0 58 34059.5 59 28716.5 60 23392.5 61 18358.5 62 13877.0 63 10140.0 64 7140.5 65 4824.0 66 3262.0 67 2228.0 68 1423.0 69 812.5 70 445.5 71 242.0 72 143.5 73 91.5 74 54.0 75 29.5 76 15.0 77 7.5 78 5.0 79 4.5 80 2.5 81 1.0 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020237122498784142 2 0.006691306632662498 3 1.6320260079664628E-4 4 0.0 5 0.1329013179154023 6 0.0 7 5.440086693221543E-5 8 0.0016864268748986782 9 0.0 10-11 0.0 12-13 0.0 14-15 5.712091027882621E-4 16-17 0.0 18-19 1.90403034262754E-4 20-21 0.0 22-23 3.2640520159329256E-4 24-25 2.7200433466107714E-5 26-27 2.720043346610771E-4 28-29 0.0031008494151362797 30-31 0.012076992458951826 32-33 0.0022304355442208327 34-35 0.004080065019916158 36-37 0.10548328098156572 38-39 0.10453126581025196 40-41 0.1187842929464924 42-43 0.12028031678712832 44-45 0.1170434652046615 46-47 0.10969934816881241 48-49 0.10953614556801576 50-51 0.1089649364652275 52-53 0.08598057018636648 54-55 0.0747739915983301 56-57 0.5886445806400371 58-59 1.6556359842150443 60-61 0.5522232002289188 62-63 0.004651274122704419 64-65 0.12985486936719823 66-67 1.9624024728458072 68-69 2.9650104504065378 70-71 2.858575154253658 72-73 2.7165888915605763 74-75 2.069436178534941 76-77 1.492406182984932 78-79 0.9865597218157268 80-81 0.057637718514682246 82-83 2.720043346610771E-4 84-85 2.9920476812718487E-4 86-87 4.3520693545772343E-4 88-89 1.6320260079664628E-4 90-91 0.26920269001406805 92-93 0.4282980253573321 94-95 2.085593236013809 96-97 2.490390086856424 98-99 1.5077200270263507 100-101 0.9880013447894305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1838206.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.83343489262187 #Duplication Level Percentage of deduplicated Percentage of total 1 71.44091539935671 34.887052908237926 2 14.327124950822467 13.992854469688945 3 5.614890751186998 8.225832057818247 4 2.7733149839249114 5.417219868169192 5 1.5603116443912692 3.809768854929041 6 0.9675378693266342 2.8348918528744966 7 0.6799439627428328 2.3242799464663397 8 0.45620587733427953 1.7822480006747978 9 0.32445952695853664 1.426002586651853 >10 1.702473439630708 14.626569303925391 >50 0.10369194441001525 3.4813260773068624 >100 0.04667351986111284 4.079805305831232 >500 0.001339707301859375 0.42290200387011073 >1k 7.814959260846354E-4 0.7726943535968905 >5k 1.1164227515494793E-4 0.5051708093155145 >10k+ 2.2328455030989585E-4 1.411381600643216 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14699 0.7996383430366346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11189 0.6086913001045585 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9266 0.5040784329939082 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2971 0.16162497565561204 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2834 0.15417205688589855 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2426 0.13197650317755466 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1868 0.10162081942937842 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.440086693221543E-5 0.0 14-15 0.0 0.0 0.0 8.160130039832314E-5 0.0 16-17 0.0 0.0 0.0 2.720043346610772E-4 5.440086693221543E-5 18-19 0.0 0.0 0.0 4.352069354577235E-4 5.440086693221543E-5 20-21 0.0 0.0 0.0 7.61612137051016E-4 5.440086693221543E-5 22-23 0.0 0.0 0.0 0.0013056208063731705 5.440086693221543E-5 24-25 0.0 0.0 0.0 0.002094433376890294 8.160130039832314E-5 26-27 0.0 0.0 0.0 0.0026384420462124483 1.0880173386443086E-4 28-29 0.0 0.0 0.0 0.005576088860552082 1.0880173386443086E-4 30-31 0.0 0.0 0.0 0.012512199394409548 1.0880173386443086E-4 32-33 0.0 0.0 0.0 0.026874028264514425 1.0880173386443086E-4 34-35 0.0 0.0 0.0 0.0468935472955697 1.0880173386443086E-4 36-37 0.0 0.0 0.0 0.07461078899753347 1.0880173386443086E-4 38-39 0.0 0.0 0.0 0.11775067647478031 1.632026007966463E-4 40-41 0.0 0.0 0.0 0.16918669615919 1.632026007966463E-4 42-43 0.0 0.0 0.0 0.22222754141810003 1.632026007966463E-4 44-45 0.0 0.0 0.0 0.28342851671684244 1.90403034262754E-4 46-47 0.0 0.0 0.0 0.35648888100680776 2.720043346610772E-4 48-49 0.0 0.0 0.0 0.41981149011590646 2.720043346610772E-4 50-51 0.0 0.0 0.0 0.48278049358994585 2.720043346610772E-4 52-53 0.0 0.0 0.0 0.5486055425779266 2.720043346610772E-4 54-55 0.0 0.0 0.0 0.620224283894188 2.720043346610772E-4 56-57 0.0 0.0 0.0 0.6762027759674378 2.720043346610772E-4 58-59 0.0 0.0 0.0 0.7150177945235735 2.720043346610772E-4 60-61 0.0 0.0 0.0 0.7453734782717498 2.720043346610772E-4 62-63 5.440086693221543E-5 0.0 0.0 0.7731451208406457 2.720043346610772E-4 64-65 5.440086693221543E-5 0.0 0.0 0.7977615131274731 2.720043346610772E-4 66-67 5.440086693221543E-5 0.0 0.0 0.8219154980453769 2.720043346610772E-4 68-69 5.440086693221543E-5 0.0 0.0 0.8553448307752233 2.720043346610772E-4 70-71 1.0880173386443086E-4 0.0 0.0 0.9078688677982772 2.720043346610772E-4 72-73 1.0880173386443086E-4 0.0 0.0 0.9912109959384312 3.264052015932926E-4 74-75 1.0880173386443086E-4 0.0 0.0 1.0978910959925057 3.264052015932926E-4 76-77 1.0880173386443086E-4 0.0 0.0 1.2812492179875379 3.264052015932926E-4 78-79 1.0880173386443086E-4 0.0 0.0 1.5595368527792859 3.264052015932926E-4 80-81 1.0880173386443086E-4 0.0 0.0 1.899243066337505 3.5360563505940036E-4 82-83 1.0880173386443086E-4 0.0 0.0 2.2349780166096727 4.3520693545772343E-4 84-85 1.0880173386443086E-4 0.0 0.0 2.4890572656165846 4.3520693545772343E-4 86-87 1.0880173386443086E-4 0.0 0.0 2.6813643302219665 4.3520693545772343E-4 88-89 1.0880173386443086E-4 0.0 0.0 2.86352563314449 4.896078023899389E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5590 0.0 53.533028 1 GTATCAA 9880 0.0 40.0512 1 ATCAACG 12960 0.0 30.309248 3 TCAACGC 13080 0.0 29.995216 4 TATCAAC 13135 0.0 29.978695 2 CAACGCA 13300 0.0 29.463686 5 AACGCAG 13600 0.0 28.775345 6 ACGCAGA 15505 0.0 25.058104 7 CGCAGAG 15470 0.0 24.690306 8 GTACATG 8510 0.0 24.10646 1 TACATGG 8545 0.0 23.288723 2 GCAGAGT 16110 0.0 22.863092 9 ACATGGG 8400 0.0 22.401361 3 GAGTACT 10050 0.0 21.666967 12-13 AGTACTT 10335 0.0 21.024017 12-13 AGAGTAC 14605 0.0 20.635242 10-11 CATGGGA 5970 0.0 20.172482 4 CAGAGTA 15465 0.0 20.095295 10-11 GTACTTT 11030 0.0 19.826805 14-15 CATGGGG 2875 0.0 18.817144 4 >>END_MODULE