FastQCFastQC Report
Fri 27 May 2016
SRR939560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744868
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56930.3262710990172322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36460.2089556344663321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.17090117991733472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37450.064.650851
GTATCAA69300.054.7952651
TATCAAC105550.037.285792
ATCAACG116000.034.0083123
GTACATG68700.033.5860021
TCAACGC119750.033.136794
CAACGCA121850.032.4998635
AACGCAG126150.031.4294766
ACGCAGA132500.029.8163647
CGCAGAG141700.027.5141018
TACATGG83550.026.1247482
GCAGAGT142450.025.6462369
ACATGGG81600.024.9542053
GAGTACT81550.023.52950712-13
CATGGGA60200.023.3059434
GTTATAG7450.022.2215271
AGTACTT87200.021.9237512-13
GTGGTAT25600.021.4213121
GTACTTT90700.021.23478514-15
AGAGTAC131350.021.14822610-11
Produced by FastQC (version 0.11.5)