FastQCFastQC Report
Fri 27 May 2016
SRR939560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744868
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140190.8034418649433654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106200.608642029081856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87830.5033618588913316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29150.16706134790711963No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28190.16155949905666217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22330.12797529669866142No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18210.10436319538211487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53900.052.772021
GTATCAA97650.040.3839841
TATCAAC126000.030.9936472
ATCAACG126000.030.950893
TCAACGC127900.030.4542314
CAACGCA130250.029.904775
AACGCAG133800.029.2011556
ACGCAGA151550.025.3456427
CGCAGAG150700.025.1758588
GTACATG83100.023.4435941
GCAGAGT156600.023.295039
TACATGG84150.022.5312252
GAGTACT94100.022.36445812-13
ACATGGG81700.021.8753643
AGTACTT96800.021.37547112-13
AGAGTAC142200.020.45029810-11
GTACTTT102700.020.42283814-15
CAGAGTA150550.020.02045210-11
CATGGGA60900.019.7451764
ACGCTCG756.5441E-419.47441368-69
Produced by FastQC (version 0.11.5)