##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939560_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1744868 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.241897954458445 31.0 31.0 34.0 30.0 34.0 2 31.45753031174851 31.0 31.0 34.0 30.0 34.0 3 31.482871483688164 31.0 31.0 34.0 30.0 34.0 4 35.344404275853535 37.0 35.0 37.0 33.0 37.0 5 35.097474422133935 37.0 35.0 37.0 32.0 37.0 6 35.10914349968021 37.0 35.0 37.0 32.0 37.0 7 35.00710311610964 37.0 35.0 37.0 32.0 37.0 8 34.96703246320065 37.0 35.0 37.0 32.0 37.0 9 36.521340296228715 38.0 35.0 39.0 32.0 39.0 10-11 36.3925772608587 38.0 35.0 39.0 32.0 39.0 12-13 36.31308414160842 38.0 35.0 39.0 32.0 39.0 14-15 37.27515433832244 39.0 36.0 40.5 32.0 41.0 16-17 37.226560977678545 39.0 36.0 40.0 32.0 41.0 18-19 37.22908838949422 39.0 36.0 40.0 31.0 41.0 20-21 37.182264503675924 39.0 36.0 40.0 31.0 41.0 22-23 37.0272791408863 39.0 36.0 40.0 31.0 41.0 24-25 36.87695258323266 39.0 36.0 40.0 31.0 41.0 26-27 36.56016558272603 38.0 35.0 40.0 30.0 41.0 28-29 36.31706610471394 38.0 35.0 40.0 30.0 41.0 30-31 36.0011502302753 38.0 34.5 40.0 29.5 41.0 32-33 35.74857611005532 38.0 34.0 40.0 29.0 41.0 34-35 35.66021240575218 38.0 34.0 40.0 29.0 41.0 36-37 35.52019321805432 38.0 34.0 40.0 27.5 41.0 38-39 35.52075228613282 38.0 34.0 40.0 27.5 41.0 40-41 35.36193396864405 38.0 34.0 40.0 27.0 41.0 42-43 35.11531531325005 38.0 34.0 40.0 26.5 41.0 44-45 34.812261156717874 38.0 33.0 40.0 25.5 41.0 46-47 34.4089326527852 38.0 33.0 40.0 24.0 41.0 48-49 34.02162656430171 38.0 33.0 40.0 23.5 41.0 50-51 33.73038963405828 37.0 33.0 40.0 23.0 41.0 52-53 33.473034922985576 37.0 32.5 40.0 22.0 41.0 54-55 33.1554384056559 37.0 32.0 40.0 20.5 41.0 56-57 32.36909984021714 36.5 30.5 39.0 14.0 40.5 58-59 29.154255508153053 34.5 24.5 38.5 6.5 40.0 60-61 28.84562098680244 33.5 24.0 38.0 6.5 40.0 62-63 30.580268536072644 34.5 28.5 38.0 4.5 40.0 64-65 30.57901256713975 35.0 29.0 38.0 2.0 40.0 66-67 25.75157404456956 30.0 17.5 36.5 2.0 39.0 68-69 18.30364904393914 14.5 7.0 30.5 2.0 36.0 70-71 16.44436742492842 12.5 7.0 27.0 2.0 34.0 72-73 17.964466653064875 16.5 7.0 29.5 2.0 34.5 74-75 19.38127382701729 21.0 7.5 31.0 2.0 35.0 76-77 21.409490001535932 25.5 8.0 32.0 2.0 35.5 78-79 23.62120200496542 29.5 10.5 34.0 2.0 36.0 80-81 25.118933925087745 31.0 16.5 34.5 2.0 36.0 82-83 26.071407980431758 32.0 23.5 35.0 2.0 36.0 84-85 26.064550155083364 33.0 23.5 35.0 2.0 36.0 86-87 25.743399787261843 32.0 22.0 35.0 2.0 35.0 88-89 25.25664806736097 32.0 19.5 34.5 2.0 35.0 90-91 23.48877336279879 31.0 6.0 34.0 2.0 35.0 92-93 21.849980628907172 30.0 2.0 34.0 2.0 35.0 94-95 18.061776019733298 24.0 2.0 33.0 2.0 35.0 96-97 15.509002400181561 6.0 2.0 31.5 2.0 34.0 98-99 15.630286073215853 2.0 2.0 32.0 2.0 34.0 100-101 14.848685401990295 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2848482031878632 1101 2 0.22088855090401793 1101 3 0.22354472592300212 1101 4 0.10162462474899314 1101 5 0.37048141615879615 1101 6 0.29485722872925635 1101 7 0.3261821734165693 1101 8 0.31554116286993406 1101 9 0.37019816388053783 1101 10-11 0.29262681944852886 1101 12-13 0.36286669756935197 1101 14-15 0.4201892732340866 1101 16-17 0.5209096479586748 1101 18-19 0.4367352347845781 1101 20-21 0.47951290664976653 1101 22-23 0.42053415621945334 1101 24-25 0.4762011138752058 1101 26-27 0.5155138828035604 1101 28-29 0.46034208170532054 1101 30-31 0.28406008342862776 1101 32-33 0.3734152827996482 1101 34-35 0.40805742997977035 1101 36-37 0.30023009347526397 1101 38-39 0.2761689607200708 1101 40-41 0.31410060115557314 1101 42-43 0.6128164180534696 1101 44-45 0.3924558778519156 1101 46-47 0.3284865086860407 1101 48-49 0.09298737319090122 1101 50-51 0.1499911852626994 1101 52-53 -0.03244364280324419 1101 54-55 0.010034365005097357 1101 56-57 -3.5330985713013376 1101 58-59 -10.069943968274647 1101 60-61 -11.381008023828787 1101 62-63 -4.063747066228363 1101 64-65 -1.3139797287078245 1101 66-67 -2.1548968298926887 1101 68-69 -4.0671491807227405 1101 70-71 -3.9912539246633045 1101 72-73 -3.176811718467345 1101 74-75 -6.175594698019346 1101 76-77 -4.72692635585836 1101 78-79 1.125573350914408 1101 80-81 1.9924481469141604 1101 82-83 0.7322427101911586 1101 84-85 0.4769634908085898 1101 86-87 0.38363356943684224 1101 88-89 0.015563744508614974 1101 90-91 1.29957037277822 1101 92-93 0.6802529903245507 1101 94-95 -8.150778961728236 1101 96-97 -8.944667403534451 1101 98-99 -8.232637166859224 1101 100-101 -8.97960121851979 1102 1 0.2521301071076998 1102 2 0.31069108553413827 1102 3 0.28690311083311215 1102 4 0.17219086406523587 1102 5 0.29777842470580396 1102 6 0.2731544583195884 1102 7 0.42210024002429236 1102 8 0.346516700742022 1102 9 0.3016286825955419 1102 10-11 0.38071350527227565 1102 12-13 0.45267383726872623 1102 14-15 0.4540973192111011 1102 16-17 0.3857729695030372 1102 18-19 0.34999599222637556 1102 20-21 0.3787033354738085 1102 22-23 0.32416202978266284 1102 24-25 0.3078885954549264 1102 26-27 0.17514492465442544 1102 28-29 0.23074069650049012 1102 30-31 0.37360933924943396 1102 32-33 0.3905369303092243 1102 34-35 0.2886129855353303 1102 36-37 0.08838493432406835 1102 38-39 0.26127340369089325 1102 40-41 0.21965523569727452 1102 42-43 0.3352403423092909 1102 44-45 0.27492253715046644 1102 46-47 0.3767540816303381 1102 48-49 0.46146751613224524 1102 50-51 0.34569005057363 1102 52-53 0.42739370615048244 1102 54-55 0.18404059105873927 1102 56-57 -3.4103007154215845 1102 58-59 -9.772880160434976 1102 60-61 -7.799746050648711 1102 62-63 -2.208516628562581 1102 64-65 -0.5360380104643774 1102 66-67 -1.8614802369071342 1102 68-69 -4.5071423652202345 1102 70-71 -4.492630840372117 1102 72-73 -3.1864473653874743 1102 74-75 -4.078413000406613 1102 76-77 -4.13536284274312 1102 78-79 1.5764538401569652 1102 80-81 1.8977633178543307 1102 82-83 0.6100932480632508 1102 84-85 0.5528826626329355 1102 86-87 0.37533247158833305 1102 88-89 0.3157663675560691 1102 90-91 1.596391953974802 1102 92-93 0.7622287492399629 1102 94-95 -8.391097264115505 1102 96-97 -9.124211685880457 1102 98-99 -8.273144277086162 1102 100-101 -8.54051431165271 1103 1 0.20837026582205453 1103 2 0.17101996424525012 1103 3 0.2559927158541484 1103 4 0.20408584855886858 1103 5 0.15438467002715583 1103 6 0.18986629810068933 1103 7 0.26924317882117776 1103 8 0.38367435306760456 1103 9 0.34603708073846207 1103 10-11 0.28006365775458875 1103 12-13 0.380610043612144 1103 14-15 0.4458990903659483 1103 16-17 0.3955686210837257 1103 18-19 0.3014623207400149 1103 20-21 0.32882599430801207 1103 22-23 0.3865742187796073 1103 24-25 0.5133686473763532 1103 26-27 0.42705026208837893 1103 28-29 0.3983122083063435 1103 30-31 0.32863673812619965 1103 32-33 0.16326730397385347 1103 34-35 0.43078360899806256 1103 36-37 0.3554313317221016 1103 38-39 0.4083395481932399 1103 40-41 0.38480583732940943 1103 42-43 0.3775034784665934 1103 44-45 0.3897982281147421 1103 46-47 0.38553863906287944 1103 48-49 0.11429553747608168 1103 50-51 -0.05556935741888225 1103 52-53 0.06691386626612683 1103 54-55 -0.0491962654087601 1103 56-57 0.39809705874234425 1103 58-59 -8.644567311726188 1103 60-61 -8.147330810161801 1103 62-63 -2.701827089549603 1103 64-65 -1.0200483412790824 1103 66-67 -2.1810823263019614 1103 68-69 -3.5688087368788075 1103 70-71 -3.7320654020454036 1103 72-73 -2.9831015126481404 1103 74-75 -3.502581630720906 1103 76-77 -0.6243860064099245 1103 78-79 2.7977414859554983 1103 80-81 2.3793829307299568 1103 82-83 0.936170534652554 1103 84-85 0.5077600222367415 1103 86-87 0.5309907268367091 1103 88-89 0.30528666595675347 1103 90-91 1.5018967128241236 1103 92-93 1.0688880499232738 1103 94-95 -7.6954391379331675 1103 96-97 -8.450335297959565 1103 98-99 -8.058782637726182 1103 100-101 -9.014588401091789 1104 1 0.2700390258786882 1104 2 0.2090605140759827 1104 3 0.31992874730702425 1104 4 0.14489571177008287 1104 5 0.25696975889713514 1104 6 0.11161175798378054 1104 7 0.21987091710531104 1104 8 0.08741523474400736 1104 9 0.2819612318936109 1104 10-11 0.27625374995045604 1104 12-13 0.3119413560190054 1104 14-15 0.34161784466265743 1104 16-17 0.31635626215529555 1104 18-19 0.35426010855945833 1104 20-21 0.42810865212050686 1104 22-23 0.22710992842022648 1104 24-25 0.3535836131326988 1104 26-27 0.2023818290465087 1104 28-29 0.22574154710478922 1104 30-31 0.3115799453234942 1104 32-33 0.31489694303130733 1104 34-35 0.29145511337745944 1104 36-37 0.12181923068940392 1104 38-39 0.17269498537109484 1104 40-41 0.26497025515022443 1104 42-43 0.37612667198872884 1104 44-45 0.29830028682132337 1104 46-47 0.28539640622094 1104 48-49 0.3360440631225856 1104 50-51 0.4004162669377749 1104 52-53 0.29663918315458204 1104 54-55 0.20703002137575055 1104 56-57 0.6580684542406843 1104 58-59 -8.296907882738562 1104 60-61 -7.877714104909867 1104 62-63 -2.445610557466857 1104 64-65 -0.5725908810689724 1104 66-67 -1.502702740198604 1104 68-69 -3.0535363577349166 1104 70-71 -3.359316183350563 1104 72-73 -2.8251615105671384 1104 74-75 -3.4364129338375804 1104 76-77 0.0656968992648963 1104 78-79 3.7165779544190087 1104 80-81 3.0912425113335225 1104 82-83 1.5100168785903278 1104 84-85 1.1268094219045217 1104 86-87 1.190985254913528 1104 88-89 0.9586993251441989 1104 90-91 2.4642598218426706 1104 92-93 1.972644213965772 1104 94-95 -6.0695605180097925 1104 96-97 -7.338543628660677 1104 98-99 -5.866768997866055 1104 100-101 -5.320727406520978 1105 1 0.21895453802949305 1105 2 0.2196442070126139 1105 3 0.3091027953046037 1105 4 0.2748052431502046 1105 5 0.3077457449672423 1105 6 0.20200277925715682 1105 7 0.1456014796005789 1105 8 0.2261369396421813 1105 9 0.17258315633905852 1105 10-11 0.25849691748773296 1105 12-13 0.14174584538232438 1105 14-15 0.3579343712733092 1105 16-17 0.3352855906434442 1105 18-19 0.47181253493540964 1105 20-21 0.30718541796198195 1105 22-23 0.5144679797488649 1105 24-25 0.5213887081436752 1105 26-27 0.5069118843118545 1105 28-29 0.5628119157928069 1105 30-31 0.60494526442411 1105 32-33 0.46618489064278634 1105 34-35 0.517616253509182 1105 36-37 0.4527612020725513 1105 38-39 0.6183305368890046 1105 40-41 0.5410660988931255 1105 42-43 0.7019802566658342 1105 44-45 0.6287539547749859 1105 46-47 0.7295093216573818 1105 48-49 0.6969419357851621 1105 50-51 0.5896499333773235 1105 52-53 0.475545610590423 1105 54-55 0.4516397571619635 1105 56-57 0.9786614211571845 1105 58-59 -7.933839141728644 1105 60-61 -7.610358514024863 1105 62-63 -1.995906757027761 1105 64-65 0.012632262242405545 1105 66-67 -1.0454699822305464 1105 68-69 -3.077184060059089 1105 70-71 -3.4317563756436975 1105 72-73 -2.346617525405506 1105 74-75 -3.0016127975668567 1105 76-77 0.4625721358460133 1105 78-79 3.872537905967196 1105 80-81 3.2605901152399497 1105 82-83 1.8223115261790923 1105 84-85 1.494318317354594 1105 86-87 1.5827333054851103 1105 88-89 1.4510463991089217 1105 90-91 2.572699143517287 1105 92-93 1.8962692045025271 1105 94-95 -6.254248041667905 1105 96-97 -7.394695684077438 1105 98-99 -5.512590975305679 1105 100-101 -5.09703617327092 1106 1 0.01044975680666127 1106 2 0.10725649330644416 1106 3 0.009097965786587281 1106 4 0.07101407150913275 1106 5 0.2452469523467471 1106 6 0.19669030944164234 1106 7 0.20697982404180948 1106 8 0.2034816706380269 1106 9 0.024302464752082642 1106 10-11 0.13319024309384275 1106 12-13 0.10413114432948589 1106 14-15 0.15350208111586028 1106 16-17 0.2839804134001298 1106 18-19 0.21829785320065298 1106 20-21 0.18016064253456676 1106 22-23 0.2980126044953124 1106 24-25 0.3239121313060451 1106 26-27 0.23685127386077198 1106 28-29 0.40445443486940036 1106 30-31 0.41275821719141703 1106 32-33 0.5169189773809322 1106 34-35 0.5401387107679554 1106 36-37 0.4754538998413125 1106 38-39 0.47260345295197936 1106 40-41 0.6067789357520041 1106 42-43 0.6206946389704839 1106 44-45 0.7007982897503595 1106 46-47 0.46019004222146975 1106 48-49 0.47942044442999787 1106 50-51 0.5335526668845247 1106 52-53 0.7474197050557905 1106 54-55 0.6353884290445038 1106 56-57 1.4319817147918812 1106 58-59 -7.636043816702081 1106 60-61 -7.228036964715486 1106 62-63 -1.590721062060119 1106 64-65 0.34032351115576276 1106 66-67 0.950602412244482 1106 68-69 -2.1037077729925393 1106 70-71 -2.110789023185472 1106 72-73 -0.4927007862960693 1106 74-75 -0.4268120151849395 1106 76-77 2.747936281504277 1106 78-79 4.930565772286137 1106 80-81 4.047257023382517 1106 82-83 2.609361173624279 1106 84-85 2.249071770459974 1106 86-87 1.9044238782659448 1106 88-89 1.7631527933907698 1106 90-91 3.168709961637635 1106 92-93 2.498031978577675 1106 94-95 -5.915358347859346 1106 96-97 -7.0507989874677595 1106 98-99 -5.156901803085068 1106 100-101 -4.657228871039683 1107 1 -0.09946265428384038 1107 2 -0.09457759143244715 1107 3 -0.03555272276297572 1107 4 0.027954233419627883 1107 5 0.023180198050219758 1107 6 0.04302766801565383 1107 7 -0.058092716282260426 1107 8 0.08600442778456596 1107 9 -0.04763345898537352 1107 10-11 -0.059360400002844926 1107 12-13 -0.06885388079243882 1107 14-15 -0.018495265419275597 1107 16-17 -0.02208263095941021 1107 18-19 0.09090389966085155 1107 20-21 -0.007782676507723352 1107 22-23 0.05266476719202018 1107 24-25 -0.054457216260992425 1107 26-27 0.09679685326202758 1107 28-29 0.0137446330193427 1107 30-31 -0.14504923462239816 1107 32-33 -0.12697436807317075 1107 34-35 0.011517529730397769 1107 36-37 0.053624851771829185 1107 38-39 0.13771161849869884 1107 40-41 -0.050260731090730815 1107 42-43 -0.042051820005227114 1107 44-45 -0.19263008512525914 1107 46-47 -0.3012954067177631 1107 48-49 -0.24370155353076228 1107 50-51 -0.23320347232410654 1107 52-53 -0.06352399480785209 1107 54-55 -0.10008876467070849 1107 56-57 0.32646226219185337 1107 58-59 -8.714843738080898 1107 60-61 -8.22051082931507 1107 62-63 -2.6284033342122513 1107 64-65 -0.8544518656731022 1107 66-67 0.04921548547273957 1107 68-69 -2.697359112623756 1107 70-71 -2.7135653336408616 1107 72-73 -1.198199355144883 1107 74-75 -1.2915332171062417 1107 76-77 1.867815155747241 1107 78-79 3.936147876139806 1107 80-81 2.9798012785082513 1107 82-83 1.4839014239836033 1107 84-85 0.9966990077853772 1107 86-87 0.8879011456885202 1107 88-89 0.6946952426862509 1107 90-91 1.8251464566071114 1107 92-93 1.0199722019109636 1107 94-95 -7.043868353633091 1107 96-97 -8.029415868909474 1107 98-99 -6.415622611900467 1107 100-101 -5.727918526212099 1108 1 -0.16666836967016962 1108 2 -0.0942068212035494 1108 3 -0.10170543042471536 1108 4 -0.07103406818409042 1108 5 -0.026239190470349172 1108 6 -0.07789918152715813 1108 7 -0.17019837692739515 1108 8 -0.11051418422199788 1108 9 -0.13924132955285273 1108 10-11 -0.15018313990107401 1108 12-13 -0.11489870268080438 1108 14-15 -0.26741235278216635 1108 16-17 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2313 64-65 0.3726917115540438 2313 66-67 -1.347102230874686 2313 68-69 -3.9601575686114465 2313 70-71 -3.1867991374292615 2313 72-73 -2.6223006710041687 2313 74-75 -3.250799280670737 2313 76-77 0.7652995033887784 2313 78-79 3.4729342347114596 2313 80-81 2.996994996341325 2313 82-83 1.4911267118278246 2313 84-85 1.2511758476007344 2313 86-87 1.188057669273178 2313 88-89 1.313661125850679 2313 90-91 2.930907320405062 2313 92-93 4.149596280013583 2313 94-95 6.021090923705479 2313 96-97 0.9833706488387062 2313 98-99 2.595804936250433 2313 100-101 3.909847431234711 2314 1 -0.23686596393997306 2314 2 -0.3029493018906706 2314 3 -0.4035858772867371 2314 4 -0.3340041798995941 2314 5 -0.20903919811901517 2314 6 -0.21917846579205502 2314 7 -0.344030886904406 2314 8 -0.6228788703342758 2314 9 -0.5009798936734171 2314 10-11 -0.3926267084983479 2314 12-13 -0.5629236317056936 2314 14-15 -0.7613201346242349 2314 16-17 -0.6343788135797865 2314 18-19 -0.4022278255363858 2314 20-21 -0.4773994480818331 2314 22-23 -0.5467510540738587 2314 24-25 -0.6089071456710045 2314 26-27 -0.6745473374510098 2314 28-29 -0.7861092187927454 2314 30-31 -0.5833807715796695 2314 32-33 -0.5055795982186169 2314 34-35 -0.47007621694883994 2314 36-37 -0.5350407283288305 2314 38-39 -0.5778484023125827 2314 40-41 -0.739812998733747 2314 42-43 -0.8714444424888654 2314 44-45 -0.9486256799787469 2314 46-47 -0.8772879102403621 2314 48-49 -0.8619119765600729 2314 50-51 -0.7706680565745998 2314 52-53 -0.6554600373798891 2314 54-55 -0.7834579565222981 2314 56-57 -0.08547993544079446 2314 58-59 3.1914353897773715 2314 60-61 3.241205935772765 2314 62-63 0.31658808872459687 2314 64-65 -0.3446901603625001 2314 66-67 -1.8085700451666256 2314 68-69 -4.631608574745982 2314 70-71 -3.635666279394904 2314 72-73 -3.2719590621308754 2314 74-75 -4.207892705766993 2314 76-77 0.21123772825896125 2314 78-79 2.987208658053312 2314 80-81 2.2457080390004904 2314 82-83 0.7673924749947005 2314 84-85 0.5185206297128495 2314 86-87 0.6609803458618551 2314 88-89 0.8216165945362874 2314 90-91 2.2875390363089316 2314 92-93 3.855083510523773 2314 94-95 6.165643285089693 2314 96-97 3.0871913402397606 2314 98-99 5.4620588071308145 2314 100-101 5.7478424035056666 2315 1 -0.4834905363858155 2315 2 -0.35377383490294534 2315 3 -0.3992685050227571 2315 4 -0.1983866842231592 2315 5 -0.21859659919934415 2315 6 -0.3880684299494135 2315 7 -0.42785706044194427 2315 8 -0.4080046688084309 2315 9 -0.7800222231621348 2315 10-11 -0.5250169699949652 2315 12-13 -0.6606798236804892 2315 14-15 -0.7865734921130141 2315 16-17 -0.688661879911038 2315 18-19 -0.5421023683753106 2315 20-21 -0.5389710566760684 2315 22-23 -0.5548441426904773 2315 24-25 -0.2795638415508961 2315 26-27 -0.37571821592101173 2315 28-29 -0.44475026570630405 2315 30-31 -0.3747445030491505 2315 32-33 -0.4434772874814783 2315 34-35 -0.5409313824126585 2315 36-37 -0.2801903520430713 2315 38-39 -0.559111584537888 2315 40-41 -0.7388577983766211 2315 42-43 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0.12077301854492717 2316 66-67 -1.9817970130428755 2316 68-69 -3.0154893821879405 2316 70-71 -2.787533686568066 2316 72-73 -1.1792155646211882 2316 74-75 5.099542664618017 2316 76-77 5.497638662456659 2316 78-79 4.539607233698291 2316 80-81 3.180005381346394 2316 82-83 1.5982569043303556 2316 84-85 1.2058554322005328 2316 86-87 0.991451792880067 2316 88-89 1.0124959789408514 2316 90-91 2.3667292555621025 2316 92-93 3.820271305342864 2316 94-95 6.111858400909128 2316 96-97 3.0487391898721423 2316 98-99 5.226554682957628 2316 100-101 5.133688616645047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 36.0 5 108.0 6 287.0 7 683.0 8 1386.0 9 2545.0 10 3959.0 11 6164.0 12 8401.0 13 11275.0 14 14094.0 15 18820.0 16 24619.0 17 31557.0 18 32628.0 19 30941.0 20 31767.0 21 32534.0 22 31810.0 23 32911.0 24 34307.0 25 35777.0 26 44002.0 27 61520.0 28 84730.0 29 109112.0 30 129930.0 31 141336.0 32 142047.0 33 138087.0 34 138547.0 35 140990.0 36 125907.0 37 78813.0 38 22405.0 39 811.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.55541746539848 20.974334339200382 15.307217478239336 22.163030717161806 2 15.776327026577789 22.475224825040552 39.10969857109286 22.638749577288802 3 18.29057360278669 27.188047678159812 31.463046445973504 23.058332273079994 4 12.810023451630725 18.680496175068832 37.64015386837285 30.869326504927592 5 12.658817241974486 38.75734199128738 34.51611811868408 14.067722648054055 6 29.64069488350981 38.47076111201535 17.731599181141497 14.156944823333342 7 26.14908405678825 34.45979867818082 22.037827503283918 17.35328976174702 8 25.604744886146115 35.518044918010986 20.373862091573688 18.503348104269204 9 26.508996669089008 16.056515449879303 20.725349997822185 36.7091378832095 10-11 24.101851830625584 27.541452992432664 28.05160619599878 20.30508898094297 12-13 25.530097405649023 25.20554563439756 28.51668435663901 20.747672603314406 14-15 22.425842272913275 25.89468393488998 27.47884933020491 24.200624461991836 16-17 21.040836075424682 29.200915943999156 27.93061818769516 21.827629792881005 18-19 21.01511840058274 27.18913632350552 31.14647274094405 20.649272534967693 20-21 21.9213717026159 26.53123330819294 31.109201383714986 20.438193605476172 22-23 21.59504329267314 26.641499528904195 30.911106169635755 20.85235100878691 24-25 21.28059044041752 26.752440157330042 31.058670947413187 20.90829845483925 26-27 21.193535094651914 26.96128128963411 30.93550969787382 20.90967391784016 28-29 21.078400609793665 26.84378765540354 31.186303713351126 20.89150802145167 30-31 21.2591121124397 26.86949110868739 30.99158467508753 20.879812103785376 32-33 21.13689899644083 26.911920632282392 30.850417528757042 21.10076284251973 34-35 21.425636246720334 26.908396290323132 30.841589095173088 20.824378367783446 36-37 21.55909812318354 26.7433583548761 30.59490166229693 21.102641859643427 38-39 21.298034196588368 27.079530597724798 30.668643874103008 20.953791331583822 40-41 21.419838645704548 26.86553894749461 30.711749048786285 21.002873358014558 42-43 21.38224066675653 27.008314380522723 30.36019422209348 21.249250730627264 44-45 21.47263950421192 27.068840557887786 30.203998048557196 21.2545218893431 46-47 21.52358697005103 27.311762826524504 29.643644398098196 21.52100580532626 48-49 21.678509383457975 27.490856283197413 29.369698665995237 21.460935667349375 50-51 21.52965324778036 27.592297020538105 29.272377220717388 21.60567251096415 52-53 21.81362443321369 27.737190070322505 28.950415265769518 21.498770230694287 54-55 21.548941440569724 27.648204770935735 29.02312073268454 21.77973305581 56-57 21.614915346000032 27.993630518453777 28.91455569339085 21.476898442155345 58-59 21.5239405496037 26.949860603571807 30.375281637386774 21.15091720943772 60-61 21.304896474811912 27.49084208001684 29.841676832547382 21.362584612623866 62-63 21.65506751161014 27.669042664219788 29.126277860467408 21.54961196370266 64-65 21.524965913760525 27.599623804832422 29.12965035742553 21.745759923981524 66-67 22.45662553158183 26.68707477077119 29.88175474908758 20.9745449485594 68-69 22.775266078961465 25.48272951192731 31.50807411514403 20.23393029396719 70-71 23.793131395233427 25.396142554352323 30.60482765060641 20.20589839980784 72-73 22.68349305080771 26.026097036068514 30.59627629949998 20.694133613623798 74-75 22.224884565630443 26.63256349979466 29.91093444768379 21.231617486891103 76-77 20.59051305196009 27.568132900664654 30.022944376202297 21.818409671172958 78-79 21.588016958721692 27.31903544748644 29.319792255371073 21.773155338420796 80-81 21.02469456451255 28.051330476925994 28.7268461582124 22.197128800349052 82-83 21.359599717227653 28.044810304971758 28.38466438549883 22.210925592301763 84-85 21.40757286811598 27.773573335205516 28.252039218327102 22.566814578351405 86-87 21.27634150052741 27.819610492957402 28.475112280650478 22.42893572586471 88-89 21.29110090021162 27.66940756246534 28.428233089332 22.611258447991037 90-91 21.378175693277154 27.59634975338581 28.864154309120842 22.1613202442162 92-93 20.941736616292232 27.844930310332057 28.614395306082898 22.59893776729282 94-95 20.77434070180415 27.124096549448453 29.63844610846906 22.463116640278336 96-97 21.172314477427467 26.619912020869602 29.98964625531792 22.21812724638501 98-99 20.86092046597927 27.062746351459875 29.114473234808138 22.96185994775272 100-101 20.15004737989334 27.34919658864768 29.51751499306238 22.983241038396596 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2038.0 1 1556.0 2 1090.0 3 1162.5 4 1185.5 5 1298.5 6 1703.5 7 2480.5 8 3439.0 9 4105.5 10 4260.0 11 4171.5 12 4210.5 13 4279.0 14 4388.5 15 4524.5 16 4643.5 17 4844.5 18 5038.5 19 5285.5 20 5628.5 21 6046.0 22 6676.5 23 7488.5 24 8419.5 25 9926.5 26 11851.5 27 14196.0 28 16722.5 29 19213.5 30 22211.0 31 25421.0 32 29022.0 33 32988.0 34 36937.0 35 41039.5 36 45367.5 37 48839.0 38 50946.0 39 52470.0 40 53917.0 41 55522.0 42 57412.5 43 59533.0 44 61469.0 45 62770.0 46 63973.5 47 65157.0 48 66239.5 49 66014.5 50 64008.5 51 61748.5 52 58870.0 53 55714.0 54 52480.5 55 48367.0 56 43781.5 57 39008.0 58 34027.0 59 29099.5 60 23837.5 61 18597.5 62 14228.0 63 10606.0 64 7574.0 65 5233.0 66 3593.5 67 2398.5 68 1489.0 69 897.0 70 529.0 71 287.5 72 159.0 73 95.0 74 59.5 75 37.0 76 17.5 77 10.0 78 5.5 79 1.5 80 2.5 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024987563529160945 2 0.010201344743556532 3 1.1462185105119699E-4 4 0.0 5 0.05507579943010016 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.43865553153591E-4 16-17 8.596638828839776E-5 18-19 4.2983194144198875E-4 20-21 0.0 22-23 0.0 24-25 5.7310925525598495E-5 26-27 5.7310925525598495E-5 28-29 8.883193456467767E-4 30-31 0.004298319414419887 32-33 0.004470252190996683 34-35 0.001490084063665561 36-37 0.08235579998028504 38-39 0.0763094973373344 40-41 0.08238445544304784 42-43 0.08613832106497454 44-45 0.08840210262323568 46-47 0.0841897495971042 48-49 0.07570773261931561 50-51 0.07054974932201176 52-53 0.05988991717425043 54-55 0.049889160670033496 56-57 0.07112285857726774 58-59 0.7722647214574397 60-61 0.3558435365884411 62-63 0.0032667227549591144 64-65 0.0016620168402423565 66-67 1.1498290988200826 68-69 2.4737974448496964 70-71 2.4147958470210926 72-73 1.881775584170264 74-75 1.3368919597356361 76-77 0.7616048893096784 78-79 0.5702150535169422 80-81 0.041206555452905316 82-83 8.596638828839776E-5 84-85 0.0011748739732747693 86-87 0.0012321848988003678 88-89 2.8655462762799247E-5 90-91 0.3391087463349663 92-93 0.05696705997244491 94-95 1.0008206924535266 96-97 1.7210184380709603 98-99 0.6632020301822258 100-101 0.6007617768220863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1744868.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.715578688992764 #Duplication Level Percentage of deduplicated Percentage of total 1 72.08414774752289 35.83705119570954 2 14.280577816811718 14.19934380351975 3 5.367319644802131 8.00518206450411 4 2.634081772011915 5.2381959843879935 5 1.4937600915548597 3.7131573687086328 6 0.9343156258963484 2.787002521176326 7 0.6194749297530725 2.1558288231198 8 0.4720130463528793 1.8773121398550208 9 0.3286436347097583 1.4704837633844625 >10 1.6407934382470186 14.337364231184562 >50 0.09638397552428468 3.2718316966623626 >100 0.046185046114695136 4.0508975769643 >500 0.0011516153492187474 0.35995101622960407 >1k 8.061307444531231E-4 0.7828805783240982 >5k 1.1516153492187473E-4 0.5028550621193912 >10k+ 2.3032306984374946E-4 1.410662174150026 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14019 0.8034418649433654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10620 0.608642029081856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8783 0.5033618588913316 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2915 0.16706134790711963 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2819 0.16155949905666217 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2233 0.12797529669866142 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1821 0.10436319538211487 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1462185105119699E-4 0.0 14-15 0.0 0.0 0.0 1.1462185105119699E-4 0.0 16-17 0.0 0.0 0.0 3.7252101591639023E-4 0.0 18-19 0.0 0.0 0.0 5.157983297303865E-4 5.7310925525598495E-5 20-21 0.0 0.0 0.0 6.304201807815835E-4 5.7310925525598495E-5 22-23 0.0 0.0 0.0 0.001260840361563167 5.7310925525598495E-5 24-25 0.0 0.0 0.0 0.0018052941540563526 5.7310925525598495E-5 26-27 0.0 0.0 0.0 0.002607647111414732 5.7310925525598495E-5 28-29 0.0 0.0 0.0 0.004556218579285081 5.7310925525598495E-5 30-31 0.0 0.0 0.0 0.011232941403017307 5.7310925525598495E-5 32-33 0.0 0.0 0.0 0.02369806770483498 5.7310925525598495E-5 34-35 0.0 0.0 0.0 0.0417796647081613 5.7310925525598495E-5 36-37 0.0 0.0 0.0 0.06791344674783423 5.7310925525598495E-5 38-39 0.0 0.0 0.0 0.10966445599323273 5.7310925525598495E-5 40-41 0.0 0.0 0.0 0.160441936008913 5.7310925525598495E-5 42-43 0.0 0.0 0.0 0.21305336564141242 5.7310925525598495E-5 44-45 0.0 0.0 0.0 0.27431874502827724 5.7310925525598495E-5 46-47 0.0 0.0 0.0 0.34188832622295784 5.7310925525598495E-5 48-49 0.0 0.0 0.0 0.4119222772152392 5.7310925525598495E-5 50-51 0.0 0.0 0.0 0.4904382451853092 5.7310925525598495E-5 52-53 0.0 0.0 0.0 0.5658021122514711 8.596638828839774E-5 54-55 0.0 0.0 0.0 0.649762618146473 1.1462185105119699E-4 56-57 0.0 0.0 0.0 0.7257282499306538 1.1462185105119699E-4 58-59 0.0 0.0 0.0 0.7833543855466432 1.1462185105119699E-4 60-61 0.0 0.0 0.0 0.8322406050199787 1.7193277657679548E-4 62-63 0.0 0.0 0.0 0.8755676647173312 1.7193277657679548E-4 64-65 0.0 0.0 0.0 0.9240240522492246 1.7193277657679548E-4 66-67 0.0 0.0 0.0 0.9674084228721026 1.7193277657679548E-4 68-69 0.0 0.0 0.0 1.0202777516694672 1.7193277657679548E-4 70-71 0.0 0.0 0.0 1.105556408851558 1.7193277657679548E-4 72-73 0.0 0.0 0.0 1.222155486833388 2.5789916486519324E-4 74-75 0.0 0.0 0.0 1.3829126329326917 2.865546276279925E-4 76-77 0.0 0.0 0.0 1.6024994440840223 2.865546276279925E-4 78-79 0.0 0.0 0.0 1.8697402898098883 2.865546276279925E-4 80-81 0.0 5.7310925525598495E-5 0.0 2.1895639097054906 2.865546276279925E-4 82-83 0.0 5.7310925525598495E-5 0.0 2.5131413952230197 2.865546276279925E-4 84-85 0.0 5.7310925525598495E-5 0.0 2.7963433337077648 2.865546276279925E-4 86-87 0.0 5.7310925525598495E-5 0.0 3.0544144313495347 2.865546276279925E-4 88-89 2.8655462762799247E-5 5.7310925525598495E-5 0.0 3.309476705401211 2.865546276279925E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5390 0.0 52.77202 1 GTATCAA 9765 0.0 40.383984 1 TATCAAC 12600 0.0 30.993647 2 ATCAACG 12600 0.0 30.95089 3 TCAACGC 12790 0.0 30.454231 4 CAACGCA 13025 0.0 29.90477 5 AACGCAG 13380 0.0 29.201155 6 ACGCAGA 15155 0.0 25.345642 7 CGCAGAG 15070 0.0 25.175858 8 GTACATG 8310 0.0 23.443594 1 GCAGAGT 15660 0.0 23.29503 9 TACATGG 8415 0.0 22.531225 2 GAGTACT 9410 0.0 22.364458 12-13 ACATGGG 8170 0.0 21.875364 3 AGTACTT 9680 0.0 21.375471 12-13 AGAGTAC 14220 0.0 20.450298 10-11 GTACTTT 10270 0.0 20.422838 14-15 CAGAGTA 15055 0.0 20.020452 10-11 CATGGGA 6090 0.0 19.745176 4 ACGCTCG 75 6.5441E-4 19.474413 68-69 >>END_MODULE