FastQCFastQC Report
Fri 27 May 2016
SRR939559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318813
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171710.7405081824191947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121370.5234143503594296No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121110.5222930870234038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87390.37687385744344193No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65930.28432650670838916No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50050.21584319218496706No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42910.18505157595718155No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37410.1613325438489434No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35580.1534405749838387No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32300.13929540674474397No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30650.13217969711227254No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29110.12553836812196584No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28970.12493461094102887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27570.11889703913165917No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.11881078810581103No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24940.10755502923262894No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23700.10220746563004435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG122950.025.7836342
GTACATG124650.025.7735231
ACATGGG125050.024.2121373
GAGTACT117400.023.28161412-13
GTCGGGA3900.023.1198582
AGTACTT123550.021.35456512-13
CATGGGA100150.021.2760774
GTACTTT130750.020.88769114-15
GGTCGGG4450.020.2614731
CTTTGCG4750.019.9817072
TTTGCGT5000.019.9317513
TTGCGTT4850.019.5697124
CGGGAGT5350.019.5148434
GTGTAGC13250.019.3399491
CATGGGG32500.019.1286414
ATGGGAG36900.018.9058655
ACTTTTT151600.018.53284616-17
AGAGTAC225550.018.38749510-11
GTAGCCC12650.018.382383
CGCGACA650.007556009618.27362694-95
Produced by FastQC (version 0.11.5)