##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939559_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2318813 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.72431282729569 31.0 31.0 33.0 27.0 34.0 2 30.895510763481145 31.0 31.0 34.0 27.0 34.0 3 30.943689292754527 31.0 31.0 34.0 27.0 34.0 4 34.55503872024178 37.0 35.0 37.0 32.0 37.0 5 34.59392758277619 37.0 35.0 37.0 32.0 37.0 6 34.73062208983648 37.0 35.0 37.0 32.0 37.0 7 34.580342183694846 37.0 35.0 37.0 30.0 37.0 8 34.5920503291986 37.0 35.0 37.0 30.0 37.0 9 35.91590826858397 38.0 35.0 39.0 30.0 39.0 10-11 35.84879009217216 38.0 35.0 39.0 30.0 39.0 12-13 35.72285475370373 38.0 35.0 39.0 30.0 39.0 14-15 36.44176309171978 38.5 36.0 40.0 30.0 41.0 16-17 36.22988119352445 38.0 35.0 40.0 29.0 41.0 18-19 36.29993557048369 38.5 35.0 40.0 29.5 41.0 20-21 36.45849967203047 39.0 36.0 40.0 30.0 41.0 22-23 36.33549255588959 39.0 35.5 40.0 29.0 41.0 24-25 36.21519285945007 39.0 35.0 40.0 29.0 41.0 26-27 36.33189955378032 39.0 35.0 40.0 30.0 41.0 28-29 36.22914310037075 39.0 35.0 40.0 29.0 41.0 30-31 35.99959850147468 38.5 35.0 40.0 29.0 41.0 32-33 35.74371952373909 38.0 35.0 40.0 27.0 41.0 34-35 35.51386549928778 38.0 34.5 40.0 27.0 41.0 36-37 35.21669923361651 38.0 34.0 40.0 25.5 41.0 38-39 35.05707920388578 38.0 34.0 40.0 25.5 41.0 40-41 34.81577212133966 38.0 34.0 40.0 24.5 41.0 42-43 34.476813783603944 38.0 33.0 40.0 24.0 41.0 44-45 34.184839398433596 38.0 33.0 40.0 23.5 41.0 46-47 33.66996885906711 38.0 33.0 40.0 20.5 41.0 48-49 33.2593730067927 38.0 32.5 40.0 18.0 41.0 50-51 32.62706759881026 37.0 31.5 39.5 15.0 40.5 52-53 32.68262404083468 37.0 31.5 39.0 15.0 40.0 54-55 32.76192215586164 37.0 32.0 40.0 13.0 41.0 56-57 32.654541569328785 37.0 31.5 40.0 12.5 41.0 58-59 32.30955579427923 37.0 31.0 39.5 8.5 41.0 60-61 31.90595101890493 36.0 31.0 39.0 8.0 40.0 62-63 31.550535554182247 36.0 30.0 39.0 7.0 40.0 64-65 30.983544166778433 35.0 29.5 38.5 3.5 40.0 66-67 30.453312319708402 35.0 29.0 38.0 2.0 40.0 68-69 29.96932201949877 34.0 28.0 38.0 2.0 40.0 70-71 29.41989673164675 34.0 28.0 37.0 2.0 39.0 72-73 28.689982331477353 34.0 26.0 36.5 2.0 39.0 74-75 28.260884556020688 33.0 26.0 36.0 2.0 39.0 76-77 27.908447554848106 33.0 26.0 36.0 2.0 38.0 78-79 27.344509022504187 33.0 25.5 35.0 2.0 37.0 80-81 26.679139715017985 32.0 24.5 35.0 2.0 37.0 82-83 26.02488449909501 31.5 22.5 35.0 2.0 36.0 84-85 25.37946483825992 31.0 20.0 34.0 2.0 36.0 86-87 24.750090240135794 31.0 18.5 34.0 2.0 35.0 88-89 23.965417866813752 30.5 12.0 34.0 2.0 35.0 90-91 22.70724676806625 29.5 4.5 34.0 2.0 35.0 92-93 21.038398094197333 27.5 2.0 33.5 2.0 35.0 94-95 20.86242443871067 27.5 2.0 33.0 2.0 34.5 96-97 20.263323735031676 27.0 2.0 33.0 2.0 34.0 98-99 19.305791583883654 26.5 2.0 33.0 2.0 34.0 100-101 17.585041786465744 22.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.894331977801631 1101 2 0.8433897041964507 1101 3 0.6732886537407943 1101 4 0.8442066129762082 1101 5 0.5762667652958413 1101 6 0.5811478933429939 1101 7 0.6503117807594307 1101 8 0.7049738677367827 1101 9 0.7951333898146657 1101 10-11 0.7802781159936814 1101 12-13 0.6607563739693489 1101 14-15 0.6971584727280558 1101 16-17 0.7034921990167433 1101 18-19 0.7015208010739613 1101 20-21 0.8077139355754355 1101 22-23 1.0228039880186444 1101 24-25 0.8280556539368931 1101 26-27 0.829538597841136 1101 28-29 0.7457448752106117 1101 30-31 1.0355745664284726 1101 32-33 0.8732112771804168 1101 34-35 0.8332964468229846 1101 36-37 1.046129063965516 1101 38-39 0.8452764984439014 1101 40-41 0.8875844364983294 1101 42-43 0.9436872991056333 1101 44-45 0.8474727381437646 1101 46-47 0.8810236849866584 1101 48-49 0.8427154530574725 1101 50-51 0.8871979322462948 1101 52-53 1.0415782746933857 1101 54-55 1.0261391225286687 1101 56-57 0.8464678756428725 1101 58-59 0.7611252163687752 1101 60-61 0.542319062235368 1101 62-63 0.6390419660105842 1101 64-65 0.7050681304350697 1101 66-67 0.5299514881653486 1101 68-69 0.46694047714255404 1101 70-71 0.33520850000646973 1101 72-73 0.5031779230778852 1101 74-75 0.3482645170509002 1101 76-77 -0.16334892517257416 1101 78-79 0.25639090435756273 1101 80-81 0.03561165671387556 1101 82-83 -0.052508982021446826 1101 84-85 -0.15490094275897803 1101 86-87 -0.6629620797553244 1101 88-89 -1.1001457492154358 1101 90-91 -4.412441919552695 1101 92-93 -10.24418704886033 1101 94-95 -8.22275532340401 1101 96-97 -4.613599515254995 1101 98-99 -3.4195539309854723 1101 100-101 -2.91148965711119 1102 1 0.8291227622183932 1102 2 0.7949007342521952 1102 3 0.7190960991572162 1102 4 0.8093364904658316 1102 5 0.7839445289919666 1102 6 0.729373605067515 1102 7 0.7148976256070938 1102 8 0.7019317155106819 1102 9 0.8581479282627313 1102 10-11 0.6141292076652434 1102 12-13 0.6682999326434782 1102 14-15 0.7212574186648126 1102 16-17 1.0579995300626663 1102 18-19 0.7769344925621127 1102 20-21 0.8293078069652893 1102 22-23 0.9500988681356901 1102 24-25 1.006191569364539 1102 26-27 0.824903243962325 1102 28-29 0.8966224884734686 1102 30-31 0.8393029416461459 1102 32-33 0.8787031218426122 1102 34-35 0.8112711101512673 1102 36-37 0.7261415313802289 1102 38-39 0.7664638908995656 1102 40-41 0.766488231332076 1102 42-43 0.620669205898821 1102 44-45 0.42705554341510776 1102 46-47 0.545741674126603 1102 48-49 0.4420761116666867 1102 50-51 0.4207337322730851 1102 52-53 0.4735097966453097 1102 54-55 0.5620215785063323 1102 56-57 0.575291302018158 1102 58-59 0.6077669997242694 1102 60-61 0.4351894526406106 1102 62-63 0.32243789400869005 1102 64-65 0.2748598936984443 1102 66-67 0.4351643615438121 1102 68-69 0.33417539002520513 1102 70-71 0.29023951398852077 1102 72-73 0.1300016688662673 1102 74-75 0.10104907806395147 1102 76-77 0.04470234578247201 1102 78-79 -0.23517040750296658 1102 80-81 -0.4020783168057491 1102 82-83 -0.23951247495829264 1102 84-85 -0.5166465868915644 1102 86-87 -0.849361521297574 1102 88-89 -1.3895855398452994 1102 90-91 -5.4692897243603795 1102 92-93 -10.91548520553761 1102 94-95 -9.282336170982138 1102 96-97 -5.67867091953925 1102 98-99 -4.229083260320991 1102 100-101 -3.402886110595368 1103 1 0.7584074836229107 1103 2 0.7351181778788138 1103 3 0.7567623459255941 1103 4 0.7597860852676845 1103 5 0.7854276882563482 1103 6 0.6396670006672522 1103 7 0.6622357518535509 1103 8 0.631881090736087 1103 9 0.6129508389650979 1103 10-11 0.616016343151685 1103 12-13 0.6069970963675217 1103 14-15 0.5377588105498816 1103 16-17 0.7452256612146044 1103 18-19 0.6629792246899555 1103 20-21 0.7773505773460982 1103 22-23 0.7708509896588467 1103 24-25 0.6184707617379459 1103 26-27 0.6042390085586078 1103 28-29 0.6893483430257348 1103 30-31 0.760324109111501 1103 32-33 0.81084326271219 1103 34-35 0.8598092605400254 1103 36-37 0.6880931549499252 1103 38-39 0.7680686873673821 1103 40-41 0.5176438773348622 1103 42-43 0.526414351333969 1103 44-45 0.7350455710158315 1103 46-47 0.9038811284958115 1103 48-49 0.6282408332489453 1103 50-51 0.5730533185658899 1103 52-53 0.657826077072194 1103 54-55 0.6966519310061372 1103 56-57 0.4478891986757816 1103 58-59 0.36767117478704137 1103 60-61 0.25923707221720704 1103 62-63 0.4043225967510651 1103 64-65 0.26852772378700607 1103 66-67 0.3423291631066938 1103 68-69 0.38277232247193993 1103 70-71 0.447417460515549 1103 72-73 0.555055100368417 1103 74-75 0.25777852211713537 1103 76-77 0.0494437898919351 1103 78-79 0.026508430289656104 1103 80-81 -0.21699694392347624 1103 82-83 -0.055133246815778136 1103 84-85 -0.07802246103168287 1103 86-87 -0.40724018698373854 1103 88-89 -0.7539998631906784 1103 90-91 -2.3223332834390327 1103 92-93 -9.723602054271286 1103 94-95 -5.85351337472612 1103 96-97 -3.1774914830027683 1103 98-99 -2.3515237876895476 1103 100-101 -2.0087586140704516 1104 1 0.7249273450485916 1104 2 0.6296162480236873 1104 3 0.4724218136072196 1104 4 0.533287097101443 1104 5 0.5995553334310415 1104 6 0.5143033100873566 1104 7 0.6987520618097278 1104 8 0.65280644956853 1104 9 0.7016352205403464 1104 10-11 0.47206506529275316 1104 12-13 0.4870840738659439 1104 14-15 0.4641211844057054 1104 16-17 0.5865807646092733 1104 18-19 0.5432094336235807 1104 20-21 0.6513184734019575 1104 22-23 0.6010472753578213 1104 24-25 0.544348158979858 1104 26-27 0.48175125442750755 1104 28-29 0.6490641249521047 1104 30-31 0.6341016162479463 1104 32-33 0.5448533575967431 1104 34-35 0.6262404189891768 1104 36-37 0.5676590548118909 1104 38-39 0.3489077555422 1104 40-41 0.4899234851990215 1104 42-43 0.46079843941041077 1104 44-45 0.46172146256869695 1104 46-47 0.19083576014811143 1104 48-49 0.14464820355329522 1104 50-51 0.2501505283170147 1104 52-53 0.15044362736234973 1104 54-55 0.2924924398714026 1104 56-57 0.38057276944383034 1104 58-59 0.0887327291245299 1104 60-61 0.1291259715296924 1104 62-63 0.12317821946438912 1104 64-65 0.4293906136110408 1104 66-67 0.25932520623359423 1104 68-69 0.0368089361122621 1104 70-71 0.2313839996420235 1104 72-73 0.06987142908707256 1104 74-75 -0.004525644493693193 1104 76-77 0.08593128603892453 1104 78-79 -0.24456535316451422 1104 80-81 -0.4615879050985434 1104 82-83 -0.39035234903109384 1104 84-85 -0.5163774103059744 1104 86-87 -0.5945576716052052 1104 88-89 -0.7346300312488978 1104 90-91 -1.205891989900028 1104 92-93 -4.508242953798202 1104 94-95 -2.1852536282876436 1104 96-97 -1.3775701346505151 1104 98-99 -1.1285973768180284 1104 100-101 -1.116969544555463 1105 1 0.8678446391755656 1105 2 0.8254687354084318 1105 3 0.7846041205475913 1105 4 0.7471526488702196 1105 5 0.5987419467305557 1105 6 0.6079808219100258 1105 7 0.6958799448247106 1105 8 0.7157463322664768 1105 9 0.8845607046755077 1105 10-11 0.7063029321919032 1105 12-13 0.7632498290639589 1105 14-15 0.9765146812750132 1105 16-17 0.9630917880961007 1105 18-19 0.7013515566850614 1105 20-21 0.8409544803766735 1105 22-23 0.8289469675720227 1105 24-25 0.8888877682372041 1105 26-27 0.7964128551778202 1105 28-29 0.8338785948472207 1105 30-31 0.9740406205014622 1105 32-33 1.0438522281681912 1105 34-35 0.9390292158505531 1105 36-37 0.7536262353355241 1105 38-39 0.9517146496797366 1105 40-41 0.7688127483624783 1105 42-43 0.9934049095168831 1105 44-45 0.8786622303159177 1105 46-47 0.978685982070914 1105 48-49 0.7537059232965007 1105 50-51 0.46008190397419924 1105 52-53 0.5808832128422594 1105 54-55 0.7558357134969427 1105 56-57 0.802684470587792 1105 58-59 0.6595868677205985 1105 60-61 0.5169690932437767 1105 62-63 0.6490084485204228 1105 64-65 0.672123467432602 1105 66-67 0.6588660043631016 1105 68-69 0.6136232871201592 1105 70-71 0.7826456902182279 1105 72-73 0.7524121809826632 1105 74-75 0.8277918636302672 1105 76-77 0.48756499452747093 1105 78-79 0.45346475172042844 1105 80-81 0.3942132956623361 1105 82-83 0.4888683122460229 1105 84-85 0.404283701685781 1105 86-87 0.08954463084387143 1105 88-89 -0.06594773091337203 1105 90-91 -1.3375992103169239 1105 92-93 -7.857384323907315 1105 94-95 -3.137047834517329 1105 96-97 -1.2863755493027043 1105 98-99 -0.8237982962124981 1105 100-101 -0.5080193186697528 1106 1 0.6855456246184843 1106 2 0.7013097964107828 1106 3 0.6830400931771123 1106 4 0.7119430110799385 1106 5 0.5611413549364173 1106 6 0.46562051438843355 1106 7 0.561897223100047 1106 8 0.6151989226868082 1106 9 0.7595653280672252 1106 10-11 0.6581134523895287 1106 12-13 0.6499872996723965 1106 14-15 0.6490002166637723 1106 16-17 0.831647968910346 1106 18-19 0.8360320142687456 1106 20-21 0.7791410540471162 1106 22-23 0.8064101785836257 1106 24-25 0.701888481560502 1106 26-27 0.6340496415242853 1106 28-29 0.8432307916187725 1106 30-31 0.6831204334522525 1106 32-33 0.6313452930806065 1106 34-35 0.6891570856558431 1106 36-37 0.7276187322312424 1106 38-39 0.5751039921013401 1106 40-41 0.7138213346613895 1106 42-43 0.6953414501630988 1106 44-45 0.4955386668697699 1106 46-47 0.5885911364921981 1106 48-49 0.34568314978985626 1106 50-51 0.3095860121879852 1106 52-53 0.5003495413408459 1106 54-55 0.5568338377208661 1106 56-57 0.5335835221320053 1106 58-59 0.4606682129954933 1106 60-61 0.44503995002431296 1106 62-63 0.44221047752890286 1106 64-65 0.6376567426432977 1106 66-67 0.4127795073088656 1106 68-69 0.38186269955312113 1106 70-71 0.41981141899686136 1106 72-73 0.542452074248363 1106 74-75 0.5863345705600693 1106 76-77 0.3747753720604301 1106 78-79 0.20965507694301166 1106 80-81 0.2160330626433904 1106 82-83 0.13995679075385326 1106 84-85 0.022937105823057635 1106 86-87 0.05150415635178618 1106 88-89 -0.17241228931341368 1106 90-91 -0.6904081189322824 1106 92-93 -4.308350480679923 1106 94-95 -1.901248251943553 1106 96-97 -1.140755618521485 1106 98-99 -0.7815006026244795 1106 100-101 -0.3258405122974004 1107 1 0.5914427344550752 1107 2 0.516904741003728 1107 3 0.5160334581716199 1107 4 0.5567494540422473 1107 5 0.5161823154983622 1107 6 0.575228036944857 1107 7 0.3822859807882679 1107 8 0.39474874578215946 1107 9 0.5295745842026633 1107 10-11 0.3149636846579682 1107 12-13 0.44894014528524906 1107 14-15 0.5244316650327079 1107 16-17 0.5646406418573449 1107 18-19 0.4665157554775803 1107 20-21 0.5669878347286073 1107 22-23 0.5460637222994009 1107 24-25 0.583110550955567 1107 26-27 0.601236987435577 1107 28-29 0.652950079847912 1107 30-31 0.7218372908645065 1107 32-33 0.5974496711461583 1107 34-35 0.47764003101003993 1107 36-37 0.7463566970925299 1107 38-39 0.6228912566970521 1107 40-41 0.7814583384416949 1107 42-43 0.5234995657177279 1107 44-45 0.29060514668361037 1107 46-47 0.2589690809380869 1107 48-49 0.24048050962861112 1107 50-51 0.30157625431810686 1107 52-53 0.3107434028794316 1107 54-55 0.368794408314038 1107 56-57 0.2463709164866259 1107 58-59 0.3770297732166057 1107 60-61 0.2314603342836925 1107 62-63 0.4204909124807621 1107 64-65 0.35299943591919103 1107 66-67 0.3831653237951649 1107 68-69 0.2898842222306506 1107 70-71 0.2652781617887534 1107 72-73 0.27567035468331724 1107 74-75 0.3310153203935471 1107 76-77 0.04015137012269676 1107 78-79 -0.02248525299063786 1107 80-81 -0.13395525905489336 1107 82-83 0.05957417498727935 1107 84-85 0.11102523807333498 1107 86-87 0.06973640749582444 1107 88-89 -0.10910110150037156 1107 90-91 -0.6089620856620996 1107 92-93 -3.8518977431994976 1107 94-95 -1.5554775272833048 1107 96-97 -0.82009229098405 1107 98-99 -0.7645367015526681 1107 100-101 -0.5790766994616483 1108 1 0.44520278028671356 1108 2 0.3928606151499494 1108 3 0.12157041541284386 1108 4 -0.10567415183147943 1108 5 -0.6654616751314464 1108 6 0.0048422163656525186 1108 7 0.2488532724572181 1108 8 0.3458403761536246 1108 9 0.3480934945916161 1108 10-11 0.276478560908032 1108 12-13 0.2248172140475475 1108 14-15 0.4909887310337453 1108 16-17 0.628775095519579 1108 18-19 0.4531769039394362 1108 20-21 0.49052967706494144 1108 22-23 0.26392835123022707 1108 24-25 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-0.13040280948224137 2311 58-59 0.08088689031372809 2311 60-61 0.16276182955376228 2311 62-63 0.10532252121380381 2311 64-65 0.31559687814739235 2311 66-67 0.3717462043122701 2311 68-69 0.3384319515839955 2311 70-71 0.3822972618525604 2311 72-73 0.2128547944425172 2311 74-75 0.3580754779656061 2311 76-77 0.48553690844765995 2311 78-79 0.5376029651689826 2311 80-81 0.36048775978162695 2311 82-83 0.48805990195485194 2311 84-85 0.5191538618145621 2311 86-87 0.7803329897356797 2311 88-89 0.9647347933036343 2311 90-91 1.6993246649690228 2311 92-93 2.8356404014449943 2311 94-95 2.1926776590012658 2311 96-97 1.9504780754819535 2311 98-99 1.680241990155377 2311 100-101 1.7009847480929317 2312 1 0.4609896213818345 2312 2 0.21368147738377985 2312 3 0.15932495182389061 2312 4 0.18146960917632526 2312 5 0.144680649379886 2312 6 0.2516300074521567 2312 7 0.32003481413085666 2312 8 -0.24673339126832872 2312 9 0.15038174823122574 2312 10-11 0.25443543241905786 2312 12-13 0.20405271672487402 2312 14-15 0.014956571927918105 2312 16-17 0.20823655554497833 2312 18-19 0.23627075898207295 2312 20-21 0.37046090689628386 2312 22-23 0.23179895207645274 2312 24-25 0.4953828132504796 2312 26-27 0.31146874610647046 2312 28-29 0.2826125746130046 2312 30-31 0.23727377395960048 2312 32-33 0.253925938241899 2312 34-35 0.24589290653891283 2312 36-37 0.4178497189926773 2312 38-39 0.39862668619611696 2312 40-41 0.3842248315790613 2312 42-43 0.5768283075252327 2312 44-45 0.37362178539959956 2312 46-47 0.5924510095287303 2312 48-49 0.33649156174544004 2312 50-51 0.43432086541253767 2312 52-53 0.41631599028433897 2312 54-55 0.4982265986341403 2312 56-57 0.3093722889513302 2312 58-59 0.4126422363694573 2312 60-61 0.5036098174717871 2312 62-63 0.35945290101297545 2312 64-65 0.6848839004800809 2312 66-67 0.6437265613726133 2312 68-69 0.42097145269175584 2312 70-71 0.5525352108835442 2312 72-73 0.629567617680383 2312 74-75 0.6915678513395349 2312 76-77 0.9154975289378946 2312 78-79 0.9970257151805377 2312 80-81 1.0950765822509894 2312 82-83 1.2148131018060155 2312 84-85 1.2033570362517487 2312 86-87 1.1602029893222472 2312 88-89 1.5682121006863348 2312 90-91 2.147213693425382 2312 92-93 3.219198924126669 2312 94-95 2.2443489018057043 2312 96-97 2.0030357676493438 2312 98-99 1.8412354518313379 2312 100-101 2.0170334334970406 2313 1 -0.1327492334562237 2313 2 -0.8777851432008958 2313 3 -0.44083749944379136 2313 4 -0.07525517472243592 2313 5 -0.8648862657128191 2313 6 -0.4921565839145785 2313 7 -1.0761428319374033 2313 8 -0.8729726107178735 2313 9 -1.00390865654267 2313 10-11 -1.811229410577397 2313 12-13 -0.6025645527456831 2313 14-15 -0.22535640566716353 2313 16-17 -1.545412623251913 2313 18-19 -1.2309707376040606 2313 20-21 -1.619257508863754 2313 22-23 -1.2564983980866131 2313 24-25 -0.5552508447402644 2313 26-27 -1.0338385509029706 2313 28-29 -2.3113528602183635 2313 30-31 -0.8268800761538273 2313 32-33 -0.4937043935116563 2313 34-35 -0.21182365123454616 2313 36-37 -0.2553002913635183 2313 38-39 -0.5835965124086755 2313 40-41 -0.4414026372083555 2313 42-43 -0.1536367896575186 2313 44-45 -0.7356553401627934 2313 46-47 -0.8341951347201544 2313 48-49 -0.41739538050622826 2313 50-51 -0.3794633176800275 2313 52-53 -0.2590302680664891 2313 54-55 -0.14657364329629985 2313 56-57 -0.6054423658565753 2313 58-59 -0.345658036813397 2313 60-61 -0.3702179106493837 2313 62-63 -0.3673953421724683 2313 64-65 -0.2253777067273397 2313 66-67 -0.3069557523354298 2313 68-69 -0.32643827379448 2313 70-71 -0.49369721882610307 2313 72-73 -0.3041386611132033 2313 74-75 -0.1791536320593039 2313 76-77 -0.0684303049512387 2313 78-79 -0.17270261556068434 2313 80-81 -0.14453107128108655 2313 82-83 -0.09480853564883418 2313 84-85 0.17994928540823807 2313 86-87 0.09466193039245141 2313 88-89 0.3607080286807758 2313 90-91 0.9658643674357528 2313 92-93 2.2571637118492482 2313 94-95 1.5839285813642974 2313 96-97 1.0949993628778927 2313 98-99 0.8238678425673847 2313 100-101 0.6938837908162938 2314 1 -0.36144706934052095 2314 2 -0.5025851702078015 2314 3 -1.6570682421774023 2314 4 -0.5111405041597692 2314 5 -0.420196029271132 2314 6 -2.5684320193227563 2314 7 -3.6730676083333726 2314 8 -1.3218656402485465 2314 9 -1.617841686923562 2314 10-11 -0.8959958446221066 2314 12-13 -1.8098630903093493 2314 14-15 -2.6532579755059587 2314 16-17 -2.0461871962521343 2314 18-19 -2.3991822522975 2314 20-21 -2.061803837894111 2314 22-23 -2.9363744570291814 2314 24-25 -1.6150585549611876 2314 26-27 -1.672321294304254 2314 28-29 -1.0257780632858982 2314 30-31 -1.169010658283888 2314 32-33 -1.954226020602583 2314 34-35 -0.7677885041250647 2314 36-37 -0.491806060650795 2314 38-39 -0.5478551885327576 2314 40-41 -0.7515344146439631 2314 42-43 -0.6516125222041538 2314 44-45 -1.1033802773498564 2314 46-47 -1.382813265854928 2314 48-49 -1.642336474823459 2314 50-51 -0.9904058179816673 2314 52-53 -0.5302550686561389 2314 54-55 -0.2252971263616601 2314 56-57 -0.17066802354032262 2314 58-59 -0.26740740899666804 2314 60-61 -0.2556219764387002 2314 62-63 -0.33325169232697505 2314 64-65 -0.3347037000593822 2314 66-67 -0.3069109752642909 2314 68-69 -0.18375414391378087 2314 70-71 -0.2748644046879889 2314 72-73 -0.3121389874466196 2314 74-75 0.022962643288582996 2314 76-77 -0.10243548189959739 2314 78-79 -0.10535000792535598 2314 80-81 0.13106172087641 2314 82-83 0.2367624721900441 2314 84-85 0.06537171526687047 2314 86-87 0.2918985208708911 2314 88-89 0.6022022606438213 2314 90-91 1.2051015380624968 2314 92-93 1.9439194981721286 2314 94-95 1.0013180109122253 2314 96-97 0.9383727627032243 2314 98-99 0.887632501783397 2314 100-101 1.10938422186398 2315 1 -7.175726269680318 2315 2 -2.9899415696391785 2315 3 -1.3468197233114871 2315 4 -0.8948718532579036 2315 5 -1.1705186904057285 2315 6 -1.4360549164262295 2315 7 -2.930336927506154 2315 8 -2.64946617543308 2315 9 -3.3263001797605085 2315 10-11 -1.9790819345401047 2315 12-13 -2.6665261551161734 2315 14-15 -4.2291481267194015 2315 16-17 -4.01878998135043 2315 18-19 -3.053220354622603 2315 20-21 -4.007757723257811 2315 22-23 -5.143471408991431 2315 24-25 -3.268689180790517 2315 26-27 -4.103727393408029 2315 28-29 -2.46923306966341 2315 30-31 -2.3261603921347813 2315 32-33 -1.3909524993826565 2315 34-35 -2.1981714563242107 2315 36-37 -2.4845511336708697 2315 38-39 -1.8178873466061916 2315 40-41 -3.0746165898855082 2315 42-43 -7.195314553813979 2315 44-45 -3.8070416354397594 2315 46-47 -2.303085880011828 2315 48-49 -5.07453378965824 2315 50-51 -6.061694410548235 2315 52-53 -6.322310988279408 2315 54-55 -3.953826537616088 2315 56-57 -3.2318656779519905 2315 58-59 -3.7078338248296205 2315 60-61 -2.461734029241722 2315 62-63 -2.9006386205566983 2315 64-65 -1.7811079624827642 2315 66-67 -2.2143608947705324 2315 68-69 -1.4947966386572809 2315 70-71 -1.429266609776974 2315 72-73 -1.849095772856895 2315 74-75 -2.387746693877201 2315 76-77 -2.2899460394602436 2315 78-79 -1.5709807416369834 2315 80-81 -1.2391322133308407 2315 82-83 -1.4301422505469255 2315 84-85 -1.6541171118963796 2315 86-87 -2.03984680813306 2315 88-89 -1.6158654064787186 2315 90-91 -0.5800739670403843 2315 92-93 0.27138715201441954 2315 94-95 0.05511957656199584 2315 96-97 -0.27764269601026825 2315 98-99 -0.06329311731353471 2315 100-101 0.05775788829916095 2316 1 -3.9053738289510846 2316 2 -2.6204625122757363 2316 3 -4.0412925759668 2316 4 -3.9385759542206493 2316 5 -3.8375421139274586 2316 6 -3.953319475199951 2316 7 -2.6095595058568115 2316 8 -1.2852401603727728 2316 9 -1.4096342339267807 2316 10-11 -2.6707744791092125 2316 12-13 -3.460302699789068 2316 14-15 -2.6206225416969318 2316 16-17 -2.2862890048990536 2316 18-19 -2.641787739083618 2316 20-21 -5.340942149140993 2316 22-23 -6.13511817512417 2316 24-25 -3.7670401091013517 2316 26-27 -3.4729989340586798 2316 28-29 -4.143818502006809 2316 30-31 -5.454401062948563 2316 32-33 -3.8787952637614396 2316 34-35 -5.497136916893211 2316 36-37 -8.754082490236314 2316 38-39 -5.419548744538581 2316 40-41 -4.726094474841904 2316 42-43 -3.8738217609234766 2316 44-45 -2.6878894480888498 2316 46-47 -4.8677252151332695 2316 48-49 -2.470480169003114 2316 50-51 -3.5715610608908044 2316 52-53 -4.539278153562854 2316 54-55 -6.1436985908787705 2316 56-57 -4.049618678668608 2316 58-59 -5.137431846243018 2316 60-61 -5.550400389609841 2316 62-63 -5.638579279593181 2316 64-65 -4.498314499749938 2316 66-67 -5.5199450156415715 2316 68-69 -4.242837248388788 2316 70-71 -5.160165424128781 2316 72-73 -7.450631829031298 2316 74-75 -7.085981296386635 2316 76-77 -6.278499351044385 2316 78-79 -5.2657572590291295 2316 80-81 -5.314228394624095 2316 82-83 -4.174051323127703 2316 84-85 -5.800025897031162 2316 86-87 -4.378402049331523 2316 88-89 -4.276119543338943 2316 90-91 -4.935252752461285 2316 92-93 -3.6119318977370263 2316 94-95 -3.6271265078180406 2316 96-97 -4.611901883802329 2316 98-99 -5.752665716523989 2316 100-101 -4.880042407929903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8713.0 3 2896.0 4 2834.0 5 3170.0 6 3610.0 7 4382.0 8 5373.0 9 6762.0 10 8291.0 11 10934.0 12 14330.0 13 17903.0 14 20123.0 15 24275.0 16 29064.0 17 34319.0 18 29227.0 19 23642.0 20 24401.0 21 26624.0 22 29478.0 23 33339.0 24 37943.0 25 44262.0 26 51147.0 27 59827.0 28 69425.0 29 81301.0 30 96907.0 31 113246.0 32 134677.0 33 161318.0 34 196498.0 35 241032.0 36 288153.0 37 277810.0 38 99650.0 39 1927.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32426553228515 20.11229582772962 14.43975526087264 21.123683379112578 2 14.513639369510454 22.242342045240683 41.141575256962994 22.102443328285872 3 19.145096383737588 27.693744067605664 30.953512473837037 22.207647074819715 4 11.447937745609249 17.867792296588924 40.434577293101185 30.24969266470064 5 11.86304821783208 39.439181580113924 34.96862208682223 13.729148115231766 6 27.817259584282255 38.897182298052606 18.97202582019515 14.313532297469985 7 25.48209336664907 35.0913298081036 22.583770965671526 16.842805859575808 8 24.962903305166606 34.1772993260875 21.276195359525023 19.583602009220872 9 26.46435812866688 16.9851214187867 21.92870848874115 34.621811963805264 10-11 23.57464765571031 27.487570000015104 28.75751362449714 20.18026871977744 12-13 26.117664627885368 25.64490072324871 28.42988518745034 19.807549461415586 14-15 22.645606694289768 26.319994492356958 28.713358904738712 22.321039908614562 16-17 20.23227010544803 29.689163440453186 28.924281620327097 21.154284833771683 18-19 19.927580099246082 28.562956171888708 31.76364558102856 19.74581814783665 20-21 21.82166894337693 26.90158546663855 31.2128667620518 20.063878827932722 22-23 20.854355854971043 27.322701733103376 31.17870024801791 20.644242163907673 24-25 20.99552126406406 27.28153423909852 31.587394940565638 20.135549556271783 26-27 20.847265423757943 27.080097266611304 32.05749715960249 20.015140150028262 28-29 20.102089661937303 28.096119137568852 31.736990326290925 20.064800874202916 30-31 20.553393735698126 27.443167121030292 31.528229346463455 20.475209796808123 32-33 20.441444042105335 27.62341663991163 31.45984035070939 20.475298967273645 34-35 21.140068824616403 27.379321050491896 31.296179670290403 20.184430454601294 36-37 20.94393770789261 27.15417011580021 31.710351373327306 20.19154080297988 38-39 20.60371038119589 27.708901896685244 31.659507293191187 20.027880428927684 40-41 20.716974248208967 27.669774325063873 31.405807942609247 20.207443484117913 42-43 20.31796795493709 27.968706215882122 30.767867374250397 20.945458454930396 44-45 20.7685456532964 27.647624384574605 30.62394335064267 20.95988661148633 46-47 21.20147583375129 27.973306486383905 30.152749959140156 20.67246772072465 48-49 21.021670990931458 27.97744256177868 30.100705162880644 20.900181284409218 50-51 20.52401303478051 28.39537394439032 30.370222234412243 20.710390786416927 52-53 20.956393878408775 28.838704995509758 29.427771435137252 20.77712969094422 54-55 20.52340104419312 28.12852983189847 29.834749333610933 21.513319790297476 56-57 21.08356241401378 28.503617358831463 29.27733141950567 21.135488807649093 58-59 21.584453828682953 28.165474925947997 29.298686494468768 20.95138475090028 60-61 21.04774802373099 28.330210550881276 30.057250651812023 20.564790773575712 62-63 20.882513008260133 28.27812149131016 29.86696095136509 20.972404549064613 64-65 20.64344326696103 28.68101231724016 29.681280834111828 20.994263581686976 66-67 20.797869021870415 28.41539480152039 29.158491174570223 21.62824500203897 68-69 20.645617964721282 28.49937656075683 29.40828535211365 21.446720122408237 70-71 21.041076927594002 28.405208817286386 29.165443290921054 21.388270964198554 72-73 20.97709706725762 27.893455808247403 29.621028449805937 21.50841867468904 74-75 20.547779524076205 29.0396709711711 29.020772936113193 21.391776568639497 76-77 20.80649602961616 28.18036223205444 29.137012530494687 21.87612920783472 78-79 20.773953099406313 28.33266523370816 29.292244918411214 21.601136748474314 80-81 20.86623042671093 28.536853780416287 29.012196215101792 21.584719577770986 82-83 20.801998029272347 28.429215151880378 29.226707549977732 21.542079268869543 84-85 20.667464362289426 28.420141576936707 28.96913496668746 21.943259094086407 86-87 20.608111464067218 28.543490983063148 29.030260887592235 21.818136665277397 88-89 20.783246641435948 28.1600568144756 28.757511653836037 22.29918489025242 90-91 20.480883748954454 28.02750451998641 29.263720534152892 22.22789119690624 92-93 20.53466501709226 28.39825298884196 29.475144415441285 21.5919375786245 94-95 20.636969446814927 28.13818816590822 28.924129687694727 22.300712699582125 96-97 20.26095617831098 28.11977578385249 29.246019307957628 22.373248729878902 98-99 20.636616144593724 28.22210007968072 28.424801964881713 22.716481810843838 100-101 20.511848750844024 27.99721269412559 28.832929101958136 22.658009453072246 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4919.0 1 3165.5 2 1468.0 3 1473.0 4 1217.0 5 1206.0 6 1889.5 7 3653.5 8 5665.5 9 6343.0 10 5320.5 11 4158.0 12 3990.0 13 4103.5 14 4363.5 15 4678.5 16 4812.5 17 4900.0 18 5233.0 19 5708.0 20 6454.5 21 7519.0 22 8663.5 23 10028.5 24 11717.0 25 13819.5 26 17149.5 27 21293.5 28 25100.0 29 29315.0 30 34408.5 31 40035.5 32 46265.0 33 52767.0 34 59968.5 35 65576.5 36 70333.5 37 75670.0 38 77481.5 39 76486.5 40 75655.5 41 76252.0 42 77342.5 43 79033.5 44 80523.5 45 81174.0 46 83417.5 47 92212.0 48 112050.5 49 110755.5 50 87349.0 51 73097.5 52 65453.0 53 60466.5 54 55546.0 55 49182.0 56 42945.0 57 37645.5 58 33170.0 59 28932.5 60 24312.5 61 19364.5 62 14460.5 63 10424.0 64 7275.5 65 5088.0 66 3366.0 67 2237.5 68 1458.0 69 842.5 70 546.5 71 389.5 72 266.0 73 187.0 74 147.5 75 99.5 76 65.0 77 51.5 78 41.0 79 27.5 80 19.0 81 16.0 82 12.0 83 8.0 84 7.0 85 4.5 86 3.0 87 3.5 88 4.0 89 3.0 90 2.5 91 2.5 92 1.5 93 0.5 94 1.0 95 1.5 96 0.5 97 1.0 98 2.0 99 2.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08180909801695954 2 0.09215922111873619 3 0.0898735689337605 4 0.09164171496364734 5 0.08866605457188656 6 0.07383087812600671 7 0.08581977071889799 8 0.08172284699111139 9 0.0772377936470082 10-11 0.08359880680330842 12-13 0.08219722763327617 14-15 0.08765260501817093 16-17 0.0855825803978156 18-19 0.0882563621991079 20-21 0.09657958619345329 22-23 0.10557125563812175 24-25 0.1285571540266507 26-27 0.13437909827140007 28-29 0.13384002935984918 30-31 0.13455160032309635 32-33 0.13151125166194946 34-35 0.1262068135722889 36-37 0.129980295953145 38-39 0.12297240010298371 40-41 0.11904797842689342 42-43 0.11911266669627951 44-45 0.10906442218497137 46-47 0.12806121062802392 48-49 0.1462170515690571 50-51 0.15622217056744117 52-53 0.16361819603391908 54-55 0.16059941012923423 56-57 0.13088593172455046 58-59 0.12389959863085122 60-61 0.11943610804321003 62-63 0.11594294149636043 64-65 0.10285434832390539 66-67 0.11615856906098078 68-69 0.1133769734773783 70-71 0.12148456990710332 72-73 0.14897708439619756 74-75 0.16204411481219053 76-77 0.1664860426433697 78-79 0.16340256846929874 80-81 0.17058296637115627 82-83 0.16956951681744065 84-85 0.1463464281078293 86-87 0.14869676856219108 88-89 0.16862075553311112 90-91 0.26002528017567605 92-93 0.533009776985035 94-95 0.16743480392769922 96-97 0.17989807716275527 98-99 0.19738547265346537 100-101 0.2048677491457914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2318813.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.39261704349879 #Duplication Level Percentage of deduplicated Percentage of total 1 72.76613300991458 36.66875874504921 2 14.242064565042684 14.3538981106996 3 5.374218087372809 8.124627419556676 4 2.5790449632826418 5.198593006906662 5 1.4789116452080042 3.726311408906885 6 0.8621119451954694 2.6066446261716663 7 0.5766537699125565 2.0341364815725327 8 0.38774018008041966 1.5631393925735877 9 0.28478333448598975 1.2915879763610794 >10 1.3229096053971445 11.655628305594286 >50 0.07687141644852973 2.676364904010406 >100 0.04306951965041968 3.8480267629021125 >500 0.0022648715591669405 0.7547251225636908 >1k 0.0027004237820834795 2.7866402318073127 >5k 2.613313337500141E-4 0.8927380223100662 >10k+ 2.613313337500141E-4 1.8181794830142417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17171 0.7405081824191947 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12137 0.5234143503594296 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12111 0.5222930870234038 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8739 0.37687385744344193 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6593 0.28432650670838916 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5005 0.21584319218496706 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4291 0.18505157595718155 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3741 0.1613325438489434 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3558 0.1534405749838387 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3230 0.13929540674474397 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3065 0.13217969711227254 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2911 0.12553836812196584 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2897 0.12493461094102887 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2757 0.11889703913165917 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2755 0.11881078810581103 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2494 0.10755502923262894 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2370 0.10220746563004435 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.509392952342427E-4 0.0 18-19 0.0 2.156275646203467E-5 0.0 2.3719032108238138E-4 0.0 20-21 0.0 4.312551292406934E-5 0.0 3.665668598545894E-4 0.0 22-23 0.0 4.312551292406934E-5 0.0 7.115709632471441E-4 0.0 24-25 0.0 4.312551292406934E-5 0.0 0.0012722026312600456 0.0 26-27 0.0 4.312551292406934E-5 0.0 0.0020484618638932934 0.0 28-29 0.0 4.312551292406934E-5 0.0 0.004334114048868969 0.0 30-31 0.0 4.312551292406934E-5 0.0 0.010306997588852573 0.0 32-33 0.0 4.312551292406934E-5 0.0 0.024064036211630693 0.0 34-35 0.0 4.312551292406934E-5 0.0 0.04502303549272839 0.0 36-37 0.0 4.312551292406934E-5 0.0 0.0751893317831149 0.0 38-39 0.0 8.625102584813868E-5 0.0 0.12191582503634403 0.0 40-41 0.0 8.625102584813868E-5 0.0 0.1748955176635632 0.0 42-43 0.0 8.625102584813868E-5 0.0 0.23085087068254317 0.0 44-45 0.0 8.625102584813868E-5 0.0 0.2981482336005534 0.0 46-47 0.0 1.7250205169627735E-4 0.0 0.3769816712257521 0.0 48-49 0.0 2.5875307754441606E-4 0.0 0.4611195469406114 0.0 50-51 0.0 2.8031583400645076E-4 0.0 0.5548959747940002 0.0 52-53 0.0 3.8812961631662406E-4 0.0 0.6508071155371304 0.0 54-55 0.0 4.312551292406934E-4 0.0 0.757585885537126 0.0 56-57 0.0 4.959433986267975E-4 0.0 0.8879974366195118 0.0 58-59 0.0 5.175061550888321E-4 0.0 1.0271634668254837 0.0 60-61 0.0 5.606316680129014E-4 0.0 1.1820918720052025 0.0 62-63 0.0 5.606316680129014E-4 0.0 1.338400293598492 0.0 64-65 0.0 5.606316680129014E-4 0.0 1.5029025626473544 0.0 66-67 0.0 6.684454503230748E-4 0.0 1.6945523420819186 0.0 68-69 0.0 6.900082067851094E-4 0.0 1.9160018509470147 0.0 70-71 0.0 6.900082067851094E-4 0.0 2.162787598655002 0.0 72-73 0.0 6.900082067851094E-4 0.0 2.4205703521586264 0.0 74-75 0.0 6.900082067851094E-4 0.0 2.6945036102523146 0.0 76-77 0.0 7.762592326332481E-4 0.0 2.9781616715103807 0.0 78-79 0.0 7.762592326332481E-4 0.0 3.3128372145576206 0.0 80-81 0.0 7.762592326332481E-4 0.0 3.680050957106071 0.0 82-83 0.0 8.409475020193522E-4 0.0 4.043167775926735 0.0 84-85 0.0 9.056357714054561E-4 0.0 4.4079880525079 0.0 86-87 0.0 9.487612843295255E-4 0.0 4.801163353836639 0.0 88-89 0.0 9.487612843295255E-4 0.0 5.224116821839449 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 12295 0.0 25.783634 2 GTACATG 12465 0.0 25.773523 1 ACATGGG 12505 0.0 24.212137 3 GAGTACT 11740 0.0 23.281614 12-13 GTCGGGA 390 0.0 23.119858 2 AGTACTT 12355 0.0 21.354565 12-13 CATGGGA 10015 0.0 21.276077 4 GTACTTT 13075 0.0 20.887691 14-15 GGTCGGG 445 0.0 20.261473 1 CTTTGCG 475 0.0 19.981707 2 TTTGCGT 500 0.0 19.931751 3 TTGCGTT 485 0.0 19.569712 4 CGGGAGT 535 0.0 19.514843 4 GTGTAGC 1325 0.0 19.339949 1 CATGGGG 3250 0.0 19.128641 4 ATGGGAG 3690 0.0 18.905865 5 ACTTTTT 15160 0.0 18.532846 16-17 AGAGTAC 22555 0.0 18.387495 10-11 GTAGCCC 1265 0.0 18.38238 3 CGCGACA 65 0.0075560096 18.273626 94-95 >>END_MODULE