FastQCFastQC Report
Fri 27 May 2016
SRR939559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318813
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171030.7375756475403579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133200.5744318321486036No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT126160.5440714710500588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101330.43699082245959464No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68670.29614289724958415No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53220.22951397978189705No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45680.19699734303714875No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38670.16676635847737614No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37020.1596506488449047No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35260.15206055857026848No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33830.14589361022212657No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33300.1436079580371509No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31310.1350259809652611No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31130.13424972173262786No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28360.12230395465266065No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA28100.12118269131663485No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27760.1197164238772165No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25090.10820191192648998No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.10815878641356591No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23870.10294059934975351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG126100.026.7682271
TACATGG127100.026.0670912
ACATGGG128500.024.3537163
GAGTACT116800.022.09283412-13
CATGGGA99000.021.770934
AGTACTT121850.020.83004412-13
GTACTTT128850.020.02650614-15
CATGGGG32550.019.806894
ATGGGAG37800.018.5498835
TACTTTT141650.017.81865914-15
ACTTTTT147350.017.76714516-17
AGAGTAC220950.017.58210210-11
GTATCAA308500.017.5644951
ATGGGAT39450.017.0582985
GGTATCA247800.016.6044941
CGTCGGG1053.341669E-416.21509264-65
AGTACAT93650.016.1813072
GCTTACG6250.016.1391560-61
AACGCAG342550.015.6838846
TACGCGG1401.0687898E-515.63598164-65
Produced by FastQC (version 0.11.5)