##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939559_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2318813 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40387603485059 31.0 31.0 34.0 30.0 34.0 2 31.613665698786406 31.0 31.0 34.0 30.0 34.0 3 31.64107799982146 31.0 31.0 34.0 30.0 34.0 4 35.45480985314469 37.0 35.0 37.0 33.0 37.0 5 35.20004114173933 37.0 35.0 37.0 32.0 37.0 6 35.234693353884076 37.0 35.0 37.0 32.0 37.0 7 35.16572703361591 37.0 35.0 37.0 32.0 37.0 8 35.131876524756414 37.0 35.0 37.0 32.0 37.0 9 36.69489648367505 39.0 35.0 39.0 32.0 39.0 10-11 36.57493208809852 38.5 35.0 39.0 32.0 39.0 12-13 36.52193730154178 38.0 35.0 39.0 32.0 39.0 14-15 37.4632704318977 39.0 36.0 41.0 32.0 41.0 16-17 37.444087772493944 39.0 36.0 41.0 32.0 41.0 18-19 37.49118988896474 39.0 36.0 41.0 32.0 41.0 20-21 37.450812980606884 39.0 36.0 41.0 32.0 41.0 22-23 37.300724982997764 39.0 36.0 40.5 31.5 41.0 24-25 37.15187770639547 39.0 36.0 40.0 31.0 41.0 26-27 36.86371540094005 39.0 36.0 40.0 30.5 41.0 28-29 36.65314408708248 38.5 35.0 40.0 30.0 41.0 30-31 36.38046729080784 38.0 35.0 40.0 30.0 41.0 32-33 36.14297552239012 38.0 35.0 40.0 30.0 41.0 34-35 36.07633862670254 38.0 35.0 40.0 30.0 41.0 36-37 35.92126575105453 38.0 35.0 40.0 30.0 41.0 38-39 35.9002289102226 38.0 35.0 40.0 29.5 41.0 40-41 35.7963798719431 38.0 35.0 40.0 29.0 41.0 42-43 35.56102648208372 38.0 34.5 40.0 27.5 41.0 44-45 35.2753928410786 38.0 34.0 40.0 27.0 41.0 46-47 34.87482819873789 38.0 34.0 40.0 25.5 41.0 48-49 34.48700326417007 38.0 33.0 40.0 24.0 41.0 50-51 34.20091615839656 38.0 33.0 40.0 23.5 41.0 52-53 33.968900467609934 38.0 33.0 40.0 23.0 41.0 54-55 33.68855315197905 38.0 33.0 40.0 22.5 41.0 56-57 31.626176625713242 36.0 29.0 39.5 13.0 40.5 58-59 26.684713687563423 32.5 16.0 38.0 2.0 40.0 60-61 27.420257476562362 32.0 18.5 38.0 2.0 40.0 62-63 30.40094630313009 34.5 28.5 38.0 2.0 40.0 64-65 30.594754514486507 35.0 29.5 38.5 2.0 40.0 66-67 23.998817067180493 26.5 16.5 35.5 2.0 38.5 68-69 16.778442246097462 13.0 7.0 28.0 2.0 35.0 70-71 15.64100748960783 12.5 7.0 25.5 2.0 33.5 72-73 16.677177719807503 13.0 7.0 28.0 2.0 34.0 74-75 17.638536613344847 17.0 7.5 29.0 2.0 34.5 76-77 18.897147376696612 21.0 7.0 30.0 2.0 34.0 78-79 21.848145150126378 26.5 9.0 33.0 2.0 35.5 80-81 25.259036196536762 30.5 20.0 34.5 2.0 36.0 82-83 26.482664837569914 32.5 25.0 35.0 2.0 36.0 84-85 26.512254114497374 33.0 25.0 35.0 2.0 36.0 86-87 26.265845283772343 33.0 24.5 35.0 2.0 36.0 88-89 25.807244913669194 33.0 22.5 35.0 2.0 35.0 90-91 24.421146077756163 32.0 10.0 34.5 2.0 35.0 92-93 21.883720463875267 30.5 2.0 34.0 2.0 35.0 94-95 14.935958397680192 4.0 2.0 31.5 2.0 34.0 96-97 12.526906870023584 2.0 2.0 28.5 2.0 34.0 98-99 12.172413860022347 2.0 2.0 29.0 2.0 34.0 100-101 11.878951213400995 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42131621023784405 1101 2 0.4487318802707385 1101 3 0.44350286625244095 1101 4 0.2886765404733751 1101 5 0.47433162362762005 1101 6 0.5082624655294126 1101 7 0.5229505921260653 1101 8 0.4941505151240051 1101 9 0.5634172982983898 1101 10-11 0.6736659806639125 1101 12-13 0.6132448399217552 1101 14-15 0.7111419743298271 1101 16-17 0.8015013287159363 1101 18-19 0.5515282101723287 1101 20-21 0.4925021106253382 1101 22-23 0.6357837419046533 1101 24-25 0.6264719013567159 1101 26-27 0.6524635800565193 1101 28-29 0.714646147622453 1101 30-31 0.707155244880866 1101 32-33 0.9760662786264191 1101 34-35 0.7000115222874896 1101 36-37 0.6407718232297341 1101 38-39 0.8501567512854322 1101 40-41 0.8644718312252593 1101 42-43 0.9402877000291383 1101 44-45 0.8280860600458695 1101 46-47 0.9285481666463511 1101 48-49 0.8890345266856272 1101 50-51 0.6711687054746065 1101 52-53 0.7867113752775552 1101 54-55 0.7009178308101909 1101 56-57 -1.7562180038982582 1101 58-59 -9.27044773892505 1101 60-61 -9.521713615479293 1101 62-63 -3.415311053027171 1101 64-65 -4.470968688370206 1101 66-67 -8.978956870260953 1101 68-69 -4.591195910895504 1101 70-71 -4.456248495215615 1101 72-73 -4.511551449929392 1101 74-75 -6.166944554906767 1101 76-77 -7.373511285465586 1101 78-79 -10.74342074056073 1101 80-81 -7.692980987528571 1101 82-83 -3.9042218613028297 1101 84-85 -3.08867989825276 1101 86-87 -2.79184100328494 1101 88-89 -2.932709513590506 1101 90-91 -9.234992771588182 1101 92-93 -16.30160634257961 1101 94-95 -10.606756147663095 1101 96-97 -8.762677951619462 1101 98-99 -8.621082007204484 1101 100-101 -8.511500812047903 1102 1 0.22728562900902816 1102 2 0.31109316740251103 1102 3 0.3492347859252014 1102 4 0.26429810807046294 1102 5 0.26613867750539555 1102 6 0.28018347775984154 1102 7 0.27349153367346446 1102 8 0.3114410677086781 1102 9 0.451071466639398 1102 10-11 0.41177170640139593 1102 12-13 0.3200648248784219 1102 14-15 0.45751380209403436 1102 16-17 0.38802879309107396 1102 18-19 0.37545302444906525 1102 20-21 0.5170157784151002 1102 22-23 0.6048921156798102 1102 24-25 0.4357165924018105 1102 26-27 0.39943559534712847 1102 28-29 0.44183515775093696 1102 30-31 0.35637912255594983 1102 32-33 0.3084684197245835 1102 34-35 0.08297237872675112 1102 36-37 0.19675025769337395 1102 38-39 0.036110560029108285 1102 40-41 0.1259835825364064 1102 42-43 0.2645422310585772 1102 44-45 0.4377014248465443 1102 46-47 0.33829850924266935 1102 48-49 0.32366818046242685 1102 50-51 0.3720447731901757 1102 52-53 0.19199122237140642 1102 54-55 0.010766573249888722 1102 56-57 -2.681771567415101 1102 58-59 -10.097923448073658 1102 60-61 -10.167087792475368 1102 62-63 -4.15872444570952 1102 64-65 -5.116694477372089 1102 66-67 -6.649368913165347 1102 68-69 -4.217888285165234 1102 70-71 -4.201940282177375 1102 72-73 -4.472726014342651 1102 74-75 -5.298371455672328 1102 76-77 -7.464312188065197 1102 78-79 -9.761957004251704 1102 80-81 -5.899162146276886 1102 82-83 -3.597914998042551 1102 84-85 -3.194325782460357 1102 86-87 -3.1258749847177647 1102 88-89 -3.3261517564887164 1102 90-91 -8.972267765663585 1102 92-93 -12.847928970422132 1102 94-95 -9.949553382163833 1102 96-97 -8.673842469975412 1102 98-99 -8.765745232452545 1102 100-101 -8.640699548820592 1103 1 0.29693079643884346 1103 2 0.2363051716059381 1103 3 0.14661349493420417 1103 4 0.1968885102471205 1103 5 0.2538344356439808 1103 6 0.168582547022325 1103 7 0.22571366209125188 1103 8 0.35467096683681376 1103 9 0.35771296903104144 1103 10-11 0.44500356431432664 1103 12-13 0.4640751275680728 1103 14-15 0.6491948726791676 1103 16-17 0.5090789165056222 1103 18-19 0.5237788558958201 1103 20-21 0.2796300377759877 1103 22-23 0.476736768863816 1103 24-25 0.4770474144915724 1103 26-27 0.5452675864130043 1103 28-29 0.5176185605859587 1103 30-31 0.5549788876498951 1103 32-33 0.47508593163096435 1103 34-35 0.5354542590416642 1103 36-37 0.6191422968068636 1103 38-39 0.487889555042905 1103 40-41 0.4817044560729613 1103 42-43 0.43007870851921126 1103 44-45 0.6097356802528324 1103 46-47 0.48785746792452045 1103 48-49 0.5722875889464447 1103 50-51 0.6725198618168591 1103 52-53 0.774749417238283 1103 54-55 0.6156143249426336 1103 56-57 -2.4557531659464225 1103 58-59 -9.891202779672597 1103 60-61 -9.911457489593179 1103 62-63 -3.9017615026594292 1103 64-65 -5.024025534960366 1103 66-67 -6.360301154022558 1103 68-69 -4.005397810635731 1103 70-71 -3.8353121432154804 1103 72-73 -4.133370343457006 1103 74-75 -4.840715180562668 1103 76-77 -6.255710493808916 1103 78-79 -8.857128766350808 1103 80-81 -4.708357622631869 1103 82-83 -2.59968827221984 1103 84-85 -2.1402827617698144 1103 86-87 -1.8888453615227903 1103 88-89 -2.018168023659559 1103 90-91 -5.231657343151113 1103 92-93 -10.066828355534033 1103 94-95 -8.324334453711687 1103 96-97 -7.629942177675842 1103 98-99 -7.7230233584175885 1103 100-101 -7.72721350062634 1104 1 0.16250499534794116 1104 2 0.1789774178768937 1104 3 0.040936671958206716 1104 4 0.11663049263719216 1104 5 0.15344461160924538 1104 6 0.1045696545179382 1104 7 0.14740476465500763 1104 8 0.09004494327650292 1104 9 0.025978746266794417 1104 10-11 0.16949588844958186 1104 12-13 0.22747957963662913 1104 14-15 0.26807637756410685 1104 16-17 0.2713660796185806 1104 18-19 0.23380396746556897 1104 20-21 0.2132570095116506 1104 22-23 0.24587667665962698 1104 24-25 0.2360432699936723 1104 26-27 0.27658074226219753 1104 28-29 0.22054684770551347 1104 30-31 0.35278099708875743 1104 32-33 0.46564957664482876 1104 34-35 0.4217172755124423 1104 36-37 0.3482039550080742 1104 38-39 0.2997508768596475 1104 40-41 0.12140326800589918 1104 42-43 0.250393030322563 1104 44-45 0.4272667891154214 1104 46-47 0.40515672613646103 1104 48-49 0.43912373218459066 1104 50-51 0.48849904551494916 1104 52-53 0.5263865274142532 1104 54-55 0.2923418133165896 1104 56-57 -2.7483688734929608 1104 58-59 -10.095628798542293 1104 60-61 -10.01966347817487 1104 62-63 -4.304316634197818 1104 64-65 -5.413809364382615 1104 66-67 -6.191195504505693 1104 68-69 -3.9636231480253343 1104 70-71 -4.007204858682465 1104 72-73 -4.4036540087075515 1104 74-75 -4.9843660074519835 1104 76-77 -6.767649043335165 1104 78-79 -8.877058206022735 1104 80-81 -4.933876941285799 1104 82-83 -2.740522884305559 1104 84-85 -2.190331259431204 1104 86-87 -2.098284564385409 1104 88-89 -2.0542729829851822 1104 90-91 -4.563075870430421 1104 92-93 -6.931017565097537 1104 94-95 -7.565002465022783 1104 96-97 -7.439355163322545 1104 98-99 -7.611145870713714 1104 100-101 -7.7107221179655525 1105 1 0.3937234572115642 1105 2 0.35636705091168963 1105 3 0.37599684209902406 1105 4 0.30744018062429745 1105 5 0.27316040511536244 1105 6 0.3920613984612942 1105 7 0.34528659076264034 1105 8 0.29472381569731 1105 9 0.37235103497778965 1105 10-11 0.4749612328262103 1105 12-13 0.4646305135617652 1105 14-15 0.6395066237927409 1105 16-17 0.584601033780956 1105 18-19 0.5959388846408089 1105 20-21 0.5965010123121033 1105 22-23 0.5582993999694494 1105 24-25 0.6679213907830857 1105 26-27 0.5788268850325409 1105 28-29 0.6195402692795824 1105 30-31 0.7434232522471405 1105 32-33 0.636941946727525 1105 34-35 0.6920440172101578 1105 36-37 0.729853548149606 1105 38-39 0.535201228237419 1105 40-41 0.5764581212462332 1105 42-43 0.5033472125106115 1105 44-45 0.6117029664951517 1105 46-47 0.7178933447198546 1105 48-49 0.622338508544523 1105 50-51 0.6226168693505159 1105 52-53 0.6668129692519855 1105 54-55 0.7139389970105512 1105 56-57 1.8419674122062268 1105 58-59 -7.914828588508211 1105 60-61 -9.320084371942535 1105 62-63 -3.2603648561734566 1105 64-65 -4.374312389401766 1105 66-67 -5.9875078542454645 1105 68-69 -3.639438846421676 1105 70-71 -3.5703641873659873 1105 72-73 -1.9124511673030984 1105 74-75 -3.805893014664475 1105 76-77 -5.897250473944659 1105 78-79 -8.530733802440267 1105 80-81 -4.102202084610941 1105 82-83 -1.6638084315830426 1105 84-85 -1.0758717669475182 1105 86-87 -0.8844744933172741 1105 88-89 -0.9668874119302551 1105 90-91 -3.803674038246328 1105 92-93 -6.197268952115053 1105 94-95 -7.723894568269725 1105 96-97 -7.633434414861474 1105 98-99 -8.07296689261538 1105 100-101 -7.987058608414946 1106 1 0.26153725341245604 1106 2 0.26806343938227073 1106 3 0.2297269945388507 1106 4 0.14999070769095368 1106 5 0.06503063311462398 1106 6 0.1403761061308444 1106 7 0.19380261411737365 1106 8 0.17391591101843318 1106 9 0.24299487529905406 1106 10-11 0.3301544906001155 1106 12-13 0.2181247409269531 1106 14-15 0.3172564850909865 1106 16-17 0.27538489682287803 1106 18-19 0.33919375241180916 1106 20-21 0.3210123858557381 1106 22-23 0.24692506375640022 1106 24-25 0.35186316246679183 1106 26-27 0.3311103121546779 1106 28-29 0.35258985845820234 1106 30-31 0.34718959923930015 1106 32-33 0.3934183938491316 1106 34-35 0.4389484583081469 1106 36-37 0.3324109442553933 1106 38-39 0.1550062532037373 1106 40-41 0.22601348305965985 1106 42-43 0.0872881916128776 1106 44-45 0.180667326749834 1106 46-47 0.30565403796441615 1106 48-49 0.2844731945501806 1106 50-51 0.13759850788053285 1106 52-53 0.14562039838198615 1106 54-55 -2.925952855576952E-4 1106 56-57 1.453902631883384 1106 58-59 -8.35346482004767 1106 60-61 -9.631155413658739 1106 62-63 -3.8774214729074927 1106 64-65 -5.468002912769709 1106 66-67 -6.95873582708634 1106 68-69 -4.232010244799525 1106 70-71 -4.213078514596443 1106 72-73 -4.795253471073144 1106 74-75 -5.270078373043383 1106 76-77 -6.803845279894304 1106 78-79 -9.137111969463593 1106 80-81 -5.171780167092248 1106 82-83 -2.810832024090505 1106 84-85 -2.2567425497537847 1106 86-87 -2.135220048256379 1106 88-89 -2.0372971765335706 1106 90-91 -4.733600063978805 1106 92-93 -7.003799823162053 1106 94-95 -7.347099239216331 1106 96-97 -7.058024518161254 1106 98-99 -6.4936458707137135 1106 100-101 -6.9123081394709285 1107 1 0.029594243524826425 1107 2 0.03667406750189173 1107 3 0.045707582606222985 1107 4 0.11523590877401091 1107 5 -0.08714559678356437 1107 6 -0.10181059098591305 1107 7 0.0931285598435494 1107 8 0.06816499621813676 1107 9 0.15454030389272333 1107 10-11 0.1109715391905084 1107 12-13 0.08467211590424739 1107 14-15 0.20857267294729098 1107 16-17 0.14834538956070986 1107 18-19 0.21473930211379155 1107 20-21 0.09016462765713129 1107 22-23 0.21374285629786272 1107 24-25 0.25298151124143686 1107 26-27 0.25430324634960755 1107 28-29 0.22446297154741757 1107 30-31 0.21524563778633876 1107 32-33 0.3436635516792137 1107 34-35 0.3106867951449672 1107 36-37 0.3044967754476957 1107 38-39 0.17498038792808046 1107 40-41 0.163042090573569 1107 42-43 0.18126608069496797 1107 44-45 0.07180929164571381 1107 46-47 0.29023720909911077 1107 48-49 0.20457380095680122 1107 50-51 0.37277632083739576 1107 52-53 0.23291648225815464 1107 54-55 0.18838870818592568 1107 56-57 1.380049138341061 1107 58-59 -8.480363149617993 1107 60-61 -9.758032376262221 1107 62-63 -3.808508333520475 1107 64-65 -5.543289290679553 1107 66-67 -6.928603213482415 1107 68-69 -3.902977727217195 1107 70-71 -3.8384872984094525 1107 72-73 -2.1723280997464016 1107 74-75 -4.794619069762213 1107 76-77 -6.529950350007367 1107 78-79 -7.403187108521891 1107 80-81 -2.5618536106341487 1107 82-83 -1.2871314730476762 1107 84-85 -1.0485801954081602 1107 86-87 -1.070833418614253 1107 88-89 -0.867134199345184 1107 90-91 -0.194567546396474 1107 92-93 -3.0213432275167627 1107 94-95 -5.850749097779122 1107 96-97 -6.364624575255174 1107 98-99 -6.165655317162212 1107 100-101 -6.504018448383636 1108 1 -0.04197414167769509 1108 2 -0.14585055635804167 1108 3 -0.08442775104409606 1108 4 -0.07947596932312706 1108 5 -0.06384365442278295 1108 6 -0.1251054366686546 1108 7 -0.1374808964154326 1108 8 -0.0030791998282424515 1108 9 -0.2094892802225914 1108 10-11 -0.07869185891829744 1108 12-13 0.02698031688991165 1108 14-15 -0.03738786565186558 1108 16-17 -0.13825782420219923 1108 18-19 -0.15302619912780813 1108 20-21 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4.945106956955346 2316 94-95 11.35595989159308 2316 96-97 13.055668464767281 2316 98-99 12.637663300485599 2316 100-101 11.571575663282763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 18.0 4 52.0 5 111.0 6 293.0 7 759.0 8 1478.0 9 2718.0 10 4586.0 11 7218.0 12 10488.0 13 14433.0 14 18152.0 15 25089.0 16 33847.0 17 44553.0 18 46996.0 19 44140.0 20 45254.0 21 44964.0 22 44154.0 23 38564.0 24 38551.0 25 43568.0 26 61003.0 27 89704.0 28 126095.0 29 168721.0 30 204036.0 31 217146.0 32 212574.0 33 191531.0 34 168514.0 35 150206.0 36 118074.0 37 73509.0 38 26244.0 39 1466.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02326724985765 21.193643563331435 15.519781907276084 21.26330727953483 2 14.90371738986985 23.223928823885352 39.65194296836555 22.22041081787925 3 18.961940790268102 27.677798248065383 31.765070674242974 21.595190287423538 4 11.887547637519713 18.81203874568583 39.714414228314226 29.58599938848023 5 11.666636440653814 39.92739711712925 35.05971364739025 13.346252794826688 6 27.85468254663054 39.52177256208241 18.413041500112342 14.210503391174708 7 25.082962705487677 35.26929510917871 22.467615974207494 17.180126211126122 8 24.99788681250248 34.83783657901682 21.01232893962839 19.151947668852305 9 25.76356955045534 16.92965323206313 21.829875889086356 35.47690132839518 10-11 23.518692537949374 27.758189211462934 28.610996229536408 20.112122021051288 12-13 25.777822532476748 25.79653900508579 28.54885236541282 19.87678609702464 14-15 22.23438611502059 26.57082228322617 28.700421145738076 22.494370456015165 16-17 20.023693156800483 29.87129621922941 28.988948224802947 21.11606239916716 18-19 19.777109762574284 28.686703241469385 31.785081956926604 19.751105039029724 20-21 21.51812155615826 27.046251681355937 31.33527800646279 20.100348756023017 22-23 20.578689318525157 27.512615312429784 31.234353497851696 20.67434187119336 24-25 20.90632146706095 27.44442954218387 31.58616067789856 20.06308831285662 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28.467693997251875 30.373617142149172 20.724056967246554 52-53 20.953227493497494 28.808867015696865 29.429262040883618 20.80864344992202 54-55 20.495221696454195 28.109133906859224 29.896730816120005 21.49891358056658 56-57 20.712190044562025 28.20353243486463 30.13025989092604 20.954017629647296 58-59 21.290263057490808 27.269776757137127 31.130905929613927 20.30905425575814 60-61 21.34402183774264 28.09495741427739 30.217208971909614 20.343811776070357 62-63 20.911872822470585 28.367004692045562 29.85298084733698 20.868141638146874 64-65 20.33505600890512 28.74688413628668 30.00441283591202 20.913647018896178 66-67 21.824647529961382 27.249247392587993 30.118433978542665 20.80767109890796 68-69 22.534241920718912 26.5363170675049 30.729028558585807 20.200412453190385 70-71 22.51383801907548 26.601700232608295 30.583153692756483 20.301308055559748 72-73 21.890743224757397 26.457312676324673 31.12405835564333 20.527885743274602 74-75 21.628905335185745 27.576072927176714 30.2785965709295 20.516425166708046 76-77 19.526005321107462 27.919952796556792 30.83603869999757 21.718003182338176 78-79 20.04890688837325 28.035529774550216 30.437157565785032 21.478405771291502 80-81 20.515200352096723 28.582422347786128 29.267392866157937 21.63498443395921 82-83 20.62225438430609 28.49197896938969 29.320313151937167 21.56545349436705 84-85 20.490760135258977 28.42374820543804 29.057521290005955 22.027970369297023 86-87 20.38114418726005 28.637586345567712 29.049522222090452 21.93174724508179 88-89 20.56616072500934 28.246560792026433 28.842124510767693 22.34515397219653 90-91 20.14856049592226 28.003696717811806 29.513168206064993 22.334574580200943 92-93 19.45544574819486 28.614818646096673 30.013940557669937 21.915795048038525 94-95 19.72807472618513 27.545067942800255 30.6046894915972 22.12216783941741 96-97 19.80020992371475 27.4982602152116 30.41643417954225 22.2850956815314 98-99 19.494094339602 28.026928542439038 29.672812083275446 22.806165034683517 100-101 19.46658364240638 27.8165526715995 29.721589319480472 22.995274366513645 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1866.0 1 1395.5 2 938.0 3 955.0 4 1004.0 5 1183.5 6 1514.0 7 2080.5 8 2747.0 9 3320.5 10 3590.0 11 3761.5 12 4126.0 13 4467.0 14 4810.0 15 5159.5 16 5524.0 17 5928.0 18 6329.5 19 6831.0 20 7379.0 21 8150.0 22 9349.5 23 10862.0 24 12691.5 25 14993.0 26 17887.0 27 21679.0 28 25710.5 29 30234.0 30 35613.5 31 41415.0 32 47978.0 33 54982.0 34 62062.0 35 68150.5 36 73205.5 37 77411.0 38 80217.0 39 81401.0 40 82112.5 41 83278.5 42 84799.0 43 86410.0 44 87802.5 45 90122.5 46 93543.5 47 97715.0 48 100169.5 49 95141.0 50 84878.5 51 74977.0 52 66158.0 53 58857.0 54 52046.0 55 45445.5 56 39228.0 57 33519.0 58 28228.0 59 23142.0 60 18610.0 61 14167.5 62 10159.5 63 7111.0 64 4834.0 65 3183.0 66 2037.5 67 1258.0 68 733.5 69 417.0 70 265.0 71 185.0 72 124.5 73 83.5 74 45.0 75 23.5 76 16.0 77 5.5 78 3.0 79 3.0 80 2.5 81 1.0 82 0.5 83 0.5 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02298589838852896 2 0.007331337197091788 3 2.156275646203467E-4 4 0.0 5 0.12622837632875097 6 0.0 7 0.0 8 0.001768146029886843 9 0.0 10-11 0.0 12-13 0.0 14-15 8.409475020193521E-4 16-17 0.0 18-19 1.509392952342427E-4 20-21 0.0 22-23 1.7250205169627735E-4 24-25 0.0 26-27 1.509392952342427E-4 28-29 0.0026306562883682298 30-31 0.011104819577947855 32-33 0.0022425266720516057 34-35 0.003342227251615374 36-37 0.09696771580976991 38-39 0.09668739997576346 40-41 0.10936630077543984 42-43 0.11227727289781454 44-45 0.1080294098747937 46-47 0.1017546477443416 48-49 0.10123714158925279 50-51 0.10022369203553715 52-53 0.0785531217911923 54-55 0.06762080426494073 56-57 0.5622704375040161 58-59 1.60597253853588 60-61 0.5324922708299462 62-63 0.004377239561793038 64-65 0.12256270773020506 66-67 1.9285298124514567 68-69 2.906918324159818 70-71 2.81346533765336 72-73 2.6647470063347067 74-75 2.0345323232188193 76-77 1.439809937239441 78-79 0.9575588889660357 80-81 0.05468315038771992 82-83 1.0781378231017335E-4 84-85 8.625102584813868E-5 86-87 2.3719032108238138E-4 88-89 6.468826938610401E-5 90-91 0.2566183646546746 92-93 0.4038488657774474 94-95 2.033777626742648 96-97 2.459836131675991 98-99 1.4449418732774053 100-101 0.961504873398588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2318813.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.30857041175333 #Duplication Level Percentage of deduplicated Percentage of total 1 70.69911335557055 33.446739822305396 2 14.94419068974053 14.13976594984517 3 5.773065116238932 8.193463726296793 4 2.8380367822719665 5.370538517810367 5 1.6277494603718876 3.850324997934847 6 1.0096486276905574 2.865901991653731 7 0.6333643642599875 2.0974493836022305 8 0.45628465376055916 1.7268939736187123 9 0.3384475202282686 1.4410321507262114 >10 1.5320751063228308 12.701383506235345 >50 0.09046965613461727 2.95815350864648 >100 0.05151963613984074 4.4821135290902205 >500 0.002560316296209292 0.8332095612933466 >1k 0.0029260757670962604 3.0655847967713603 >5k 1.8287973544351628E-4 0.5272826614715959 >10k+ 3.6575947088703255E-4 2.3001619226981456 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17103 0.7375756475403579 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13320 0.5744318321486036 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12616 0.5440714710500588 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10133 0.43699082245959464 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6867 0.29614289724958415 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5322 0.22951397978189705 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4568 0.19699734303714875 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3867 0.16676635847737614 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3702 0.1596506488449047 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3526 0.15206055857026848 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3383 0.14589361022212657 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3330 0.1436079580371509 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3131 0.1350259809652611 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3113 0.13424972173262786 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2836 0.12230395465266065 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2810 0.12118269131663485 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2776 0.1197164238772165 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2509 0.10820191192648998 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.10815878641356591 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2387 0.10294059934975351 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.312551292406934E-5 0.0 0.0 0.0 6 0.0 4.312551292406934E-5 0.0 0.0 0.0 7 0.0 4.312551292406934E-5 0.0 0.0 0.0 8 0.0 4.312551292406934E-5 0.0 0.0 0.0 9 0.0 4.312551292406934E-5 0.0 0.0 0.0 10-11 0.0 4.312551292406934E-5 0.0 0.0 0.0 12-13 0.0 4.312551292406934E-5 0.0 0.0 0.0 14-15 0.0 4.312551292406934E-5 0.0 0.0 0.0 16-17 0.0 4.312551292406934E-5 0.0 1.509392952342427E-4 0.0 18-19 0.0 6.4688269386104E-5 0.0 2.3719032108238138E-4 0.0 20-21 0.0 1.0781378231017335E-4 0.0 3.665668598545894E-4 0.0 22-23 0.0 1.509392952342427E-4 0.0 7.115709632471441E-4 0.0 24-25 0.0 1.7250205169627735E-4 0.0 0.0013584536571081842 0.0 26-27 0.0 1.7250205169627735E-4 0.0 0.0021347128897414324 0.0 28-29 0.0 1.7250205169627735E-4 0.0 0.004441927831179142 0.0 30-31 0.0 1.7250205169627735E-4 0.0 0.010608876179321058 0.0 32-33 0.0 1.7250205169627735E-4 0.0 0.02460310512318156 0.0 34-35 0.0 1.7250205169627735E-4 0.0 0.046208987098140294 0.0 36-37 0.0 1.7250205169627735E-4 0.0 0.07678497576130547 0.0 38-39 0.0 1.7250205169627735E-4 0.0 0.12433085376009191 0.0 40-41 0.0 1.7250205169627735E-4 0.0 0.17828087042810264 0.0 42-43 0.0 1.7250205169627735E-4 0.0 0.23499091992325383 0.0 44-45 0.0 1.7250205169627735E-4 0.0 0.30377611303714447 0.0 46-47 0.0 2.156275646203467E-4 0.0 0.3797848295658166 0.0 48-49 0.0 2.5875307754441606E-4 0.0 0.4483543951150869 0.0 50-51 0.0 3.018785904684854E-4 0.0 0.5187999204765541 0.0 52-53 0.0 3.018785904684854E-4 0.0 0.58838293557954 0.0 54-55 0.0 3.665668598545894E-4 0.0 0.6640682107612818 0.0 56-57 0.0 4.312551292406934E-4 0.0 0.7298993062398735 0.0 58-59 0.0 4.5281788570272806E-4 0.0 0.7722269971748477 0.0 60-61 0.0 4.7438064216476277E-4 0.0 0.8089483714296927 0.0 62-63 0.0 4.7438064216476277E-4 0.0 0.8420040770859918 0.0 64-65 2.156275646203467E-5 4.7438064216476277E-4 0.0 0.873162260173632 2.156275646203467E-5 66-67 4.312551292406934E-5 4.7438064216476277E-4 0.0 0.9025954227443093 4.312551292406934E-5 68-69 4.312551292406934E-5 4.7438064216476277E-4 0.0 0.9439743523949538 4.312551292406934E-5 70-71 4.312551292406934E-5 4.7438064216476277E-4 0.0 1.007498232932108 4.312551292406934E-5 72-73 4.312551292406934E-5 4.7438064216476277E-4 0.0 1.1004121505270152 4.312551292406934E-5 74-75 4.312551292406934E-5 4.7438064216476277E-4 0.0 1.225928955892519 4.312551292406934E-5 76-77 4.312551292406934E-5 4.7438064216476277E-4 0.0 1.4179021766740139 4.312551292406934E-5 78-79 4.312551292406934E-5 5.175061550888321E-4 0.0 1.723403310228121 4.312551292406934E-5 80-81 4.312551292406934E-5 5.175061550888321E-4 0.0 2.091501125791515 4.312551292406934E-5 82-83 4.312551292406934E-5 5.175061550888321E-4 0.0 2.4507797739619366 4.312551292406934E-5 84-85 4.312551292406934E-5 5.390689115508668E-4 0.0 2.72911183437388 4.312551292406934E-5 86-87 4.312551292406934E-5 6.037571809369708E-4 0.0 2.9439847025180557 4.312551292406934E-5 88-89 4.312551292406934E-5 6.037571809369708E-4 0.0 3.1510949783358986 4.312551292406934E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12610 0.0 26.768227 1 TACATGG 12710 0.0 26.067091 2 ACATGGG 12850 0.0 24.353716 3 GAGTACT 11680 0.0 22.092834 12-13 CATGGGA 9900 0.0 21.77093 4 AGTACTT 12185 0.0 20.830044 12-13 GTACTTT 12885 0.0 20.026506 14-15 CATGGGG 3255 0.0 19.80689 4 ATGGGAG 3780 0.0 18.549883 5 TACTTTT 14165 0.0 17.818659 14-15 ACTTTTT 14735 0.0 17.767145 16-17 AGAGTAC 22095 0.0 17.582102 10-11 GTATCAA 30850 0.0 17.564495 1 ATGGGAT 3945 0.0 17.058298 5 GGTATCA 24780 0.0 16.604494 1 CGTCGGG 105 3.341669E-4 16.215092 64-65 AGTACAT 9365 0.0 16.181307 2 GCTTACG 625 0.0 16.13915 60-61 AACGCAG 34255 0.0 15.683884 6 TACGCGG 140 1.0687898E-5 15.635981 64-65 >>END_MODULE