FastQCFastQC Report
Fri 27 May 2016
SRR939558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297943
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74560.3244640968030974No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51530.22424403042199045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50060.21784700490830278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37290.16227556558191392No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29470.1282451305363101No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG105950.031.0141261
TACATGG122350.025.7117252
GTACCGG2000.001171107425.1989771
ACATGGG120650.024.5863083
GAGTACT108950.023.84737812-13
CATGGGA94700.022.5923714
AGTACTT117000.021.7830412-13
GAACCGT1954.495978E-721.782096
GTACTTT122300.021.11102514-15
GTGTAGC10850.020.4378781
GTATCAA296600.020.3562871
ATGGGAT38550.019.8335825
CTTTGCG4300.019.7726572
TTTGCGT4550.019.7243733
TTGCGTT4800.019.678974
CATGGGG31800.019.3076674
ACTTTTT146350.018.3388216-17
GTATAAT13750.018.3265271
TACACCG1300.00785012518.1525335
AGAGTAC220900.018.03137610-11
Produced by FastQC (version 0.11.5)