FastQCFastQC Report
Fri 27 May 2016
SRR939558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297943
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168140.7316978706608476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135060.5877430380126922No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT127710.5557579104442539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101870.44330951638051946No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65630.28560325473695386No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52760.2295966436069128No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43180.18790718481702984No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36870.16044784400657458No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35320.15370268104996512No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35080.15265826872119978No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33120.1441289013696162No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33050.14382428110705966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32960.14343262648377267No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.14064752694039842No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28860.12559058253403152No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26670.11606032003404784No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26540.11549459668929996No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.10970681170072538No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.10779205576465561No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23700.10313571746557683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120300.022.5551131
TACATGG119300.022.2236672
GAGTACT123600.022.10546112-13
TACGCGG1257.2868716E-921.38811164-65
AGTACTT128550.021.3642912-13
TTACGCG1157.31161E-821.07021362-63
ACATGGG119850.020.7041823
GTACTTT137050.019.88444314-15
CGCGTGA1001.0535392E-519.44439366-67
CATGGGA93100.018.7989944
GTATCAA315250.018.2469961
GCTTACG3800.018.24219760-61
TACTTTT153550.018.00871814-15
TACGCGC951.5649074E-417.90870764-65
AGAGTAC231900.017.90165710-11
ACTTTTT156800.017.8609716-17
ATGGGAG35450.016.6342855
CATGGGG30950.016.3092334
TCAACGC358250.016.0256184
AACGCAG359450.016.0155476
Produced by FastQC (version 0.11.5)