##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939558_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297943 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.332775878252853 31.0 31.0 34.0 30.0 34.0 2 31.535736961273624 31.0 31.0 34.0 30.0 34.0 3 31.571615135797536 31.0 31.0 34.0 30.0 34.0 4 35.3991726513669 37.0 35.0 37.0 33.0 37.0 5 35.160322514527124 37.0 35.0 37.0 32.0 37.0 6 35.18187570361841 37.0 35.0 37.0 32.0 37.0 7 35.08865929224528 37.0 35.0 37.0 32.0 37.0 8 35.05517630332867 37.0 35.0 37.0 32.0 37.0 9 36.60733099123868 39.0 35.0 39.0 32.0 39.0 10-11 36.48109961822378 38.0 35.0 39.0 32.0 39.0 12-13 36.419730167371426 38.0 35.0 39.0 32.0 39.0 14-15 37.37143762921883 39.0 36.0 41.0 32.0 41.0 16-17 37.344627782325325 39.0 36.0 41.0 32.0 41.0 18-19 37.378994387589245 39.0 36.0 41.0 32.0 41.0 20-21 37.34048603468406 39.0 36.0 41.0 31.5 41.0 22-23 37.19423175422541 39.0 36.0 40.0 31.0 41.0 24-25 37.044442573205686 39.0 36.0 40.0 31.0 41.0 26-27 36.728041339580656 39.0 35.5 40.0 30.0 41.0 28-29 36.52404824662753 38.0 35.0 40.0 30.0 41.0 30-31 36.24338484461973 38.0 35.0 40.0 30.0 41.0 32-33 36.00446225167465 38.0 35.0 40.0 30.0 41.0 34-35 35.92220738286372 38.0 35.0 40.0 29.5 41.0 36-37 35.786759071047456 38.0 34.5 40.0 29.0 41.0 38-39 35.776656122453865 38.0 35.0 40.0 29.0 41.0 40-41 35.65342417109563 38.0 34.5 40.0 28.5 41.0 42-43 35.42766117349299 38.0 34.0 40.0 27.0 41.0 44-45 35.13691701665359 38.0 34.0 40.0 26.5 41.0 46-47 34.74263678428925 38.0 33.5 40.0 24.5 41.0 48-49 34.35176090094488 38.0 33.0 40.0 24.0 41.0 50-51 34.0795822611788 38.0 33.0 40.0 23.0 41.0 52-53 33.85546181954905 38.0 33.0 40.0 23.0 41.0 54-55 33.58742427466652 37.5 33.0 40.0 22.0 41.0 56-57 32.773556176110546 37.0 31.5 40.0 15.0 41.0 58-59 29.67284741179394 34.5 25.5 39.0 7.5 40.0 60-61 29.3757475707622 34.0 24.5 38.0 7.5 40.0 62-63 31.175627506861574 35.0 29.5 38.5 7.5 40.0 64-65 31.227589413662564 35.0 30.0 39.0 2.0 40.0 66-67 26.366917717280195 31.5 18.0 36.5 2.0 39.5 68-69 19.02241113030219 18.5 7.5 31.5 2.0 36.5 70-71 17.09890367167506 13.5 7.5 28.5 2.0 35.0 72-73 18.716746673002767 17.5 7.5 30.5 2.0 35.0 74-75 20.11832234306943 22.0 7.5 31.5 2.0 36.0 76-77 21.997959044240872 27.0 10.0 33.0 2.0 35.5 78-79 24.11833169926321 30.0 11.5 34.5 2.0 36.5 80-81 25.62814025413163 31.5 19.5 35.0 2.0 36.5 82-83 26.66710183847032 32.5 25.0 35.0 2.0 36.0 84-85 26.674332870745708 33.0 25.0 35.0 2.0 36.0 86-87 26.38574586053701 33.0 25.0 35.0 2.0 36.0 88-89 25.90196558400274 33.0 23.0 35.0 2.0 35.0 90-91 24.063330552585505 31.5 10.0 34.5 2.0 35.0 92-93 22.438262828973564 31.0 2.0 34.0 2.0 35.0 94-95 18.75544062668221 25.0 2.0 34.0 2.0 35.0 96-97 16.164203594257994 8.5 2.0 32.0 2.0 35.0 98-99 16.345630418160937 6.0 2.0 32.0 2.0 35.0 100-101 15.56614872518596 2.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2676334497129531 1101 2 0.26053717861379155 1101 3 0.1528796785209252 1101 4 0.1538263571500451 1101 5 0.35406042195761245 1101 6 0.26213456594469164 1101 7 0.3058542784766658 1101 8 0.3532167629682803 1101 9 0.37531399563886936 1101 10-11 0.372970424683551 1101 12-13 0.3733188907047591 1101 14-15 0.47596117465850796 1101 16-17 0.36938904638143555 1101 18-19 0.3800418284961822 1101 20-21 0.42776355064395233 1101 22-23 0.5335450470038126 1101 24-25 0.3183096092319886 1101 26-27 0.4457321053718033 1101 28-29 0.4404992052779022 1101 30-31 0.3053713137238958 1101 32-33 0.4308637982072909 1101 34-35 0.30290769076209045 1101 36-37 0.19921378269817325 1101 38-39 0.20931377909052173 1101 40-41 0.4958418388256334 1101 42-43 0.6712924904464401 1101 44-45 0.5134455519170373 1101 46-47 0.3754499469832524 1101 48-49 0.08828134725043668 1101 50-51 0.185884325404011 1101 52-53 0.12038529647296059 1101 54-55 0.08115274982896636 1101 56-57 -3.192723957939773 1101 58-59 -10.10455630881588 1101 60-61 -11.585096126448743 1101 62-63 -4.070885117615177 1101 64-65 -1.1774623808340579 1101 66-67 -1.740791328464109 1101 68-69 -4.118069117992146 1101 70-71 -4.125703104269437 1101 72-73 -3.248172652107204 1101 74-75 -5.855886495789207 1101 76-77 -4.513259779377716 1101 78-79 1.2815701802903448 1101 80-81 1.9703713811238295 1101 82-83 0.7426983606277311 1101 84-85 0.47371828751679956 1101 86-87 0.39551040034463725 1101 88-89 0.2423819533713747 1101 90-91 1.4172374953188545 1101 92-93 0.6145586221593824 1101 94-95 -8.539947444004232 1101 96-97 -9.390272709325188 1101 98-99 -8.684779413791611 1101 100-101 -9.379328790750225 1102 1 0.2998974265948142 1102 2 0.3469125062327585 1102 3 0.3078604032232448 1102 4 0.25561459158165434 1102 5 0.3612634571296809 1102 6 0.339665861574602 1102 7 0.32477156270635277 1102 8 0.3135127044642445 1102 9 0.39136350657782515 1102 10-11 0.39435714641667374 1102 12-13 0.3810088486106835 1102 14-15 0.5026771649078299 1102 16-17 0.4516979276810318 1102 18-19 0.5086150899009851 1102 20-21 0.4983459639967549 1102 22-23 0.45199007116666934 1102 24-25 0.308549160554378 1102 26-27 0.24631005883004775 1102 28-29 0.3839507970936893 1102 30-31 0.45590252993103064 1102 32-33 0.24405897663361742 1102 34-35 0.30517123998283324 1102 36-37 0.20170399231322023 1102 38-39 0.30472192053638025 1102 40-41 0.2589805048895002 1102 42-43 0.3852751762804729 1102 44-45 0.2577903445319407 1102 46-47 0.30515773224849596 1102 48-49 0.4640042472816006 1102 50-51 0.5356536938707208 1102 52-53 0.4124815080063726 1102 54-55 0.3264200685770149 1102 56-57 -3.096040775407893 1102 58-59 -9.327743483869796 1102 60-61 -7.783551047911832 1102 62-63 -2.134968303812723 1102 64-65 -0.4886585489904114 1102 66-67 -1.7559739092156939 1102 68-69 -4.670361076130231 1102 70-71 -4.239032079532297 1102 72-73 -3.056171136964945 1102 74-75 -3.7371952610093615 1102 76-77 -3.857970527723584 1102 78-79 1.8955840242917859 1102 80-81 2.2908612363910876 1102 82-83 0.9145258787880586 1102 84-85 0.7173262811141008 1102 86-87 0.6412121006030667 1102 88-89 0.5038674037290853 1102 90-91 1.6951054213457013 1102 92-93 0.8951568589736745 1102 94-95 -8.407041975440379 1102 96-97 -9.13293862656665 1102 98-99 -8.246633263658119 1102 100-101 -8.91914465209027 1103 1 0.134064610608025 1103 2 0.13775143228451014 1103 3 0.17383201328438602 1103 4 0.25412739345627244 1103 5 0.15488541386011434 1103 6 0.18405323071529978 1103 7 0.31052588389393065 1103 8 0.37842171188044915 1103 9 0.32342507076111104 1103 10-11 0.37573898873343836 1103 12-13 0.35586389459328416 1103 14-15 0.40213600435128427 1103 16-17 0.42293531642381055 1103 18-19 0.3888542420001997 1103 20-21 0.30883602279636335 1103 22-23 0.3304832410284746 1103 24-25 0.45788072776027633 1103 26-27 0.508568844272169 1103 28-29 0.35752871480350024 1103 30-31 0.3550239441541905 1103 32-33 0.2835610869752472 1103 34-35 0.30318742242754837 1103 36-37 0.39827949625640713 1103 38-39 0.323449061827354 1103 40-41 0.3107143833791852 1103 42-43 0.2268072746546821 1103 44-45 0.2291293183003944 1103 46-47 0.36830379887962295 1103 48-49 0.164209961115084 1103 50-51 -0.011093706566811079 1103 52-53 0.24491551182303795 1103 54-55 0.2062584502926441 1103 56-57 0.5178317987300574 1103 58-59 -8.243933260658732 1103 60-61 -7.315202181989768 1103 62-63 -2.3273298580536377 1103 64-65 -0.6344826063861149 1103 66-67 -1.6825433147321398 1103 68-69 -3.3464203120046747 1103 70-71 -3.814888660762243 1103 72-73 -2.8361590864204924 1103 74-75 -3.768894278045387 1103 76-77 -0.46381854987477666 1103 78-79 3.0838845051497437 1103 80-81 2.707806666112372 1103 82-83 1.1913262031950964 1103 84-85 0.8863731575325104 1103 86-87 0.8399460785163875 1103 88-89 0.7221919956515634 1103 90-91 1.8900477506454472 1103 92-93 1.14456854700423 1103 94-95 -6.853741592675984 1103 96-97 -8.41354664370343 1103 98-99 -7.836366176508143 1103 100-101 -9.03037202531432 1104 1 0.26505151350065503 1104 2 0.2677450124858147 1104 3 0.23791087334745953 1104 4 0.15169570295388013 1104 5 0.25219986878591527 1104 6 0.1120656486360403 1104 7 0.22250365513695414 1104 8 0.25589233077580786 1104 9 0.2432557538740383 1104 10-11 0.27050602053771655 1104 12-13 0.27200890501934083 1104 14-15 0.4509551138759278 1104 16-17 0.3381684347520988 1104 18-19 0.4597970157122475 1104 20-21 0.30102766113095214 1104 22-23 0.29846420944221563 1104 24-25 0.33334454760903753 1104 26-27 0.2528299456772487 1104 28-29 0.2852353192326049 1104 30-31 0.3279140840026713 1104 32-33 0.27591901343574676 1104 34-35 0.23593668571729864 1104 36-37 0.12352989941128101 1104 38-39 0.2345793184659044 1104 40-41 0.07469523299237579 1104 42-43 0.06491174994302185 1104 44-45 0.14255872450328155 1104 46-47 0.064296222407485 1104 48-49 -7.417402621214819E-4 1104 50-51 0.1559569948296513 1104 52-53 0.08716201381604805 1104 54-55 0.061749336430487745 1104 56-57 0.41999391969838484 1104 58-59 -8.435561559209724 1104 60-61 -7.919222829785642 1104 62-63 -2.300630496214918 1104 64-65 -0.27088700996984727 1104 66-67 -1.313032087591047 1104 68-69 -3.0040199241404775 1104 70-71 -3.5722648201690763 1104 72-73 -2.495614059545133 1104 74-75 -3.1663356276195813 1104 76-77 0.24596044847344345 1104 78-79 3.603317124842107 1104 80-81 2.969878466028753 1104 82-83 1.550200538654547 1104 84-85 1.0575579710368679 1104 86-87 1.0789702462679784 1104 88-89 0.952434714304129 1104 90-91 2.3816019783619424 1104 92-93 1.9199686094724306 1104 94-95 -5.999430063050422 1104 96-97 -7.4100615285305445 1104 98-99 -5.517962519670698 1104 100-101 -5.019897913226005 1105 1 0.20641296940323528 1105 2 0.19250081476093683 1105 3 0.20846323433011804 1105 4 0.10098786569371043 1105 5 0.16898970737847208 1105 6 0.21119251286176421 1105 7 0.19186657836533527 1105 8 0.23803105267990787 1105 9 0.16922919937969283 1105 10-11 0.19403119865290108 1105 12-13 0.20514253503367996 1105 14-15 0.28214581927405646 1105 16-17 0.33778389475055803 1105 18-19 0.26471952837787427 1105 20-21 0.33706696802624236 1105 22-23 0.28301922718842576 1105 24-25 0.2821258066085477 1105 26-27 0.1196351129845965 1105 28-29 0.2894937051133297 1105 30-31 0.1849824324422329 1105 32-33 0.058111598183991475 1105 34-35 0.23917526734722827 1105 36-37 0.10293861967915063 1105 38-39 0.10253642306479094 1105 40-41 0.08052447159035836 1105 42-43 0.18874188374434908 1105 44-45 0.1768262947922139 1105 46-47 0.338869642624374 1105 48-49 0.013455550313416609 1105 50-51 0.1716540635274555 1105 52-53 0.2387670897120202 1105 54-55 0.15042599157510494 1105 56-57 0.7380694792846185 1105 58-59 -7.789979554431778 1105 60-61 -7.32770543445881 1105 62-63 -1.9617170993669113 1105 64-65 -0.27901828017942876 1105 66-67 -1.8478049525767197 1105 68-69 -3.1324630155055466 1105 70-71 -3.3653535329624873 1105 72-73 -2.4974962943098884 1105 74-75 -3.4226181971320564 1105 76-77 0.18751765999672543 1105 78-79 3.761299603151535 1105 80-81 3.128227295012703 1105 82-83 1.6904873659118707 1105 84-85 1.3252635981736134 1105 86-87 1.16419229147591 1105 88-89 1.1596831050294618 1105 90-91 2.5438693653152775 1105 92-93 2.3459850843552097 1105 94-95 -6.039367575099851 1105 96-97 -7.396744056504046 1105 98-99 -5.58845585748913 1105 100-101 -5.014968445346353 1106 1 0.16717330774559613 1106 2 0.1973545141223525 1106 3 0.20055267361973605 1106 4 0.10405218280207151 1106 5 0.26635446922288253 1106 6 0.23093536372048362 1106 7 0.24852289395271754 1106 8 0.2770172185183597 1106 9 0.28439458676231055 1106 10-11 0.2789765292971893 1106 12-13 0.2365163705372666 1106 14-15 0.21615302899338218 1106 16-17 0.3104920875117685 1106 18-19 0.2691035148978571 1106 20-21 0.1994019627512884 1106 22-23 0.265690178431484 1106 24-25 0.30754994612071584 1106 26-27 0.2750373698429627 1106 28-29 0.3662339725514201 1106 30-31 0.39510064350732677 1106 32-33 0.34850037875725803 1106 34-35 0.41320573903585256 1106 36-37 0.2717854746534414 1106 38-39 0.20017332012684363 1106 40-41 0.1666589958569844 1106 42-43 0.22118562072007109 1106 44-45 0.27905593963711084 1106 46-47 0.15035076650553947 1106 48-49 0.045567505996388036 1106 50-51 0.026721556789311762 1106 52-53 0.05946166825658139 1106 54-55 -0.02504344167534356 1106 56-57 0.6671465681088051 1106 58-59 -8.392540980569432 1106 60-61 -7.71081963059747 1106 62-63 -2.1808139086746827 1106 64-65 -0.21172445988560185 1106 66-67 0.46600796005296274 1106 68-69 -2.0880826077500636 1106 70-71 -2.298061576608502 1106 72-73 -0.8949069090745283 1106 74-75 -1.0066893399881351 1106 76-77 2.0745181707162423 1106 78-79 4.285994942183553 1106 80-81 3.3803382068893555 1106 82-83 1.9050342980532768 1106 84-85 1.6422601200618594 1106 86-87 1.4760480672663085 1106 88-89 1.3235122339358156 1106 90-91 2.5694090690979543 1106 92-93 2.1651568240448285 1106 94-95 -5.934383027161182 1106 96-97 -7.182756306316719 1106 98-99 -5.155658344730462 1106 100-101 -4.5243682939944385 1107 1 0.06450344705622157 1107 2 0.010078211468687925 1107 3 0.07443069795592905 1107 4 0.09760739490967296 1107 5 0.07714399995988686 1107 6 0.12142102956452305 1107 7 0.13870202059673176 1107 8 0.20165303891397457 1107 9 0.19334076049101157 1107 10-11 0.1083912779707461 1107 12-13 0.1310231523722294 1107 14-15 -0.03433671971263408 1107 16-17 0.1285491450210472 1107 18-19 -0.01682680642142742 1107 20-21 0.18696034962486152 1107 22-23 0.16420549987410737 1107 24-25 0.17552859190875125 1107 26-27 0.07377661581820405 1107 28-29 -0.09586299899057593 1107 30-31 -0.04769482522990387 1107 32-33 0.02207742995725681 1107 34-35 -0.024773137183977667 1107 36-37 0.08884461643455666 1107 38-39 0.16730977015300397 1107 40-41 -0.17796254750378893 1107 42-43 -0.1531586223147272 1107 44-45 -0.08078099903427471 1107 46-47 -0.1216876818511281 1107 48-49 -0.2830258831425496 1107 50-51 -0.013354589560194086 1107 52-53 -0.2561307426831547 1107 54-55 -0.2694499532235497 1107 56-57 0.17139681768915693 1107 58-59 -8.499368106740906 1107 60-61 -7.800102053000376 1107 62-63 -2.486947094358573 1107 64-65 -0.7068178853743419 1107 66-67 0.41767359248114033 1107 68-69 -2.6784098436200523 1107 70-71 -2.7123651273353033 1107 72-73 -1.2731429341974376 1107 74-75 -1.2808858145763757 1107 76-77 1.8372756539077066 1107 78-79 3.941297048005623 1107 80-81 2.8235394465475103 1107 82-83 1.2523101885261205 1107 84-85 0.8710024622615187 1107 86-87 0.7216258863559304 1107 88-89 0.6480456420026179 1107 90-91 1.9836401636295165 1107 92-93 1.3042947884298108 1107 94-95 -6.689700078594857 1107 96-97 -8.03353500351376 1107 98-99 -6.265408871478076 1107 100-101 -5.439540426546596 1108 1 -0.050552969007192416 1108 2 -0.07549626177917901 1108 3 -0.13080800872098308 1108 4 -0.02905450301567214 1108 5 0.004777507866222663 1108 6 -0.011705208960172797 1108 7 -0.12266333913149907 1108 8 -0.04257265567088808 1108 9 -0.09288608668009601 1108 10-11 -0.0088578323135593 1108 12-13 -0.19214206176616244 1108 14-15 -0.24206069047333756 1108 16-17 -0.3329175715236161 1108 18-19 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2312 28-29 -0.4539986825484803 2312 30-31 -0.3459830680658911 2312 32-33 -0.41072514150524597 2312 34-35 -0.5653431810356295 2312 36-37 -0.33097971675958604 2312 38-39 -0.4522905902362524 2312 40-41 -0.2818554220092153 2312 42-43 -0.3758263478212953 2312 44-45 -0.3777330891818025 2312 46-47 -0.31865876805187554 2312 48-49 -0.37261446934636666 2312 50-51 -0.280474457258272 2312 52-53 -0.34838436341355816 2312 54-55 -0.2537294563443524 2312 56-57 0.6137857374642834 2312 58-59 3.5078371528850845 2312 60-61 3.4340647902076427 2312 62-63 0.7835802764896975 2312 64-65 0.0974275564913043 2312 66-67 -1.8720599974392265 2312 68-69 -4.680669547373466 2312 70-71 -4.492536707157914 2312 72-73 -6.88540264407367 2312 74-75 -7.3436715207274865 2312 76-77 -5.114244152807071 2312 78-79 -0.7144621695957731 2312 80-81 1.0252777614491109 2312 82-83 0.2856012692898098 2312 84-85 0.08454917387785699 2312 86-87 0.027294424973174358 2312 88-89 0.12739757503854676 2312 90-91 1.757235909860615 2312 92-93 3.2320889685656624 2312 94-95 5.211957843662457 2312 96-97 0.3308657804522852 2312 98-99 -1.0546989395271122 2312 100-101 -0.7530442631064531 2313 1 -0.3310485101354743 2313 2 -0.4392382803744539 2313 3 -0.4064133413464823 2313 4 -0.3634532919851381 2313 5 -0.4167083910432936 2313 6 -0.27098305925312616 2313 7 -0.3544444586954967 2313 8 -0.376393690781768 2313 9 -0.4297873595649975 2313 10-11 -0.47030158989316106 2313 12-13 -0.5083711311258483 2313 14-15 -0.5109220861659409 2313 16-17 -0.601681178266233 2313 18-19 -0.6439291756049599 2313 20-21 -0.4937959962048808 2313 22-23 -0.4542668143730779 2313 24-25 -0.4699471501744057 2313 26-27 -0.5488488840361327 2313 28-29 -0.6882729729668071 2313 30-31 -0.7516092700827386 2313 32-33 -0.7804007442483467 2313 34-35 -0.5717435405217159 2313 36-37 -0.8143619652317682 2313 38-39 -0.845546996457891 2313 40-41 -0.7951793100334896 2313 42-43 -0.7189872710823124 2313 44-45 -0.7105051064877443 2313 46-47 -0.8491296231482792 2313 48-49 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2314 8 -0.6287779876437654 2314 9 -0.661970442018557 2314 10-11 -0.5533382874010542 2314 12-13 -0.673671283711748 2314 14-15 -0.8498120931317104 2314 16-17 -0.9019724579388466 2314 18-19 -0.7025961108122871 2314 20-21 -0.7253414967862781 2314 22-23 -0.6546647570644168 2314 24-25 -0.7174034578443553 2314 26-27 -0.8977883126966475 2314 28-29 -0.8518811980156116 2314 30-31 -0.6968707279400732 2314 32-33 -0.8570261344146388 2314 34-35 -0.7272149378315262 2314 36-37 -0.8760074421085875 2314 38-39 -0.6812336246643937 2314 40-41 -0.7105614731801353 2314 42-43 -0.793643346165311 2314 44-45 -0.7547911126121107 2314 46-47 -0.6466031973967645 2314 48-49 -0.5774411411577915 2314 50-51 -0.7800145713504634 2314 52-53 -0.7737509539714225 2314 54-55 -0.5798878997302666 2314 56-57 0.07704600524055394 2314 58-59 3.1347698936645934 2314 60-61 3.217746409562004 2314 62-63 0.5549400053201801 2314 64-65 -0.2762188658788496 2314 66-67 -2.2181568517682457 2314 68-69 -4.760366611884248 2314 70-71 -3.4433313322942425 2314 72-73 -2.750475457579812 2314 74-75 -4.423595279983559 2314 76-77 0.1571904359009757 2314 78-79 2.8465830012018323 2314 80-81 2.168386430041636 2314 82-83 0.6914556440904782 2314 84-85 0.27567701687244295 2314 86-87 0.4089155016470656 2314 88-89 0.6123445453890461 2314 90-91 2.022442171862931 2314 92-93 3.6059082258499195 2314 94-95 5.991900019505966 2314 96-97 3.213522129196882 2314 98-99 5.407170264025732 2314 100-101 5.759679725427823 2315 1 -0.3822827823709751 2315 2 -0.3589649883604409 2315 3 -0.4765709052783329 2315 4 -0.3926559485414529 2315 5 -0.43908649520275134 2315 6 -0.5005684027284261 2315 7 -0.49802162993937316 2315 8 -0.6833239097683759 2315 9 -0.6382606144818084 2315 10-11 -0.42861837488914745 2315 12-13 -0.6175556016857158 2315 14-15 -0.7771688085879092 2315 16-17 -0.6325707192573233 2315 18-19 -0.5036800603050224 2315 20-21 -0.58960873870614 2315 22-23 -0.6225694097670456 2315 24-25 -0.5325561929348908 2315 26-27 -0.348603878250735 2315 28-29 -0.4370328742702796 2315 30-31 -0.4976558380724967 2315 32-33 -0.6740013530515441 2315 34-35 -0.5286114582142716 2315 36-37 -0.48136708833358455 2315 38-39 -0.6471455387269245 2315 40-41 -0.424715949873125 2315 42-43 -0.49730360049863975 2315 44-45 -0.5696051630942875 2315 46-47 -0.34171204239097364 2315 48-49 -0.31723960675062557 2315 50-51 -0.3819477643629128 2315 52-53 -0.39586576511685934 2315 54-55 -0.5160882759265562 2315 56-57 0.3179781554504757 2315 58-59 3.3983122378129202 2315 60-61 3.432242587037507 2315 62-63 0.7455292720898257 2315 64-65 0.1870785014297276 2315 66-67 -1.6546651158473118 2315 68-69 -4.644709032738646 2315 70-71 -3.581155489658528 2315 72-73 -3.0112914977844607 2315 74-75 -0.5522262972708383 2315 76-77 1.8588636664505032 2315 78-79 3.629486802232126 2315 80-81 2.6447367672419695 2315 82-83 0.8588058446105009 2315 84-85 0.751860829510246 2315 86-87 0.7856219220182439 2315 88-89 0.911748485877709 2315 90-91 2.737380695090092 2315 92-93 4.1288525801354545 2315 94-95 6.493672815551793 2315 96-97 3.7944777515915646 2315 98-99 5.979718618456678 2315 100-101 6.339229731293489 2316 1 -0.471946762259563 2316 2 -0.4727278528495269 2316 3 -0.5178820403305622 2316 4 -0.2759294708816711 2316 5 -0.5518570719237985 2316 6 -0.51050403979648 2316 7 -0.4482068537177639 2316 8 -0.5013508307678052 2316 9 -0.816298731026059 2316 10-11 -0.6687370470098628 2316 12-13 -0.49872586982588274 2316 14-15 -0.6510727690037115 2316 16-17 -0.6782019703349178 2316 18-19 -0.6137858879754319 2316 20-21 -0.5817001266756066 2316 22-23 -0.8744314624646208 2316 24-25 -0.7976026191121832 2316 26-27 -0.6435432597083377 2316 28-29 -0.7497011795913053 2316 30-31 -0.6281198413860167 2316 32-33 -0.5850416750068845 2316 34-35 -0.7435225694489773 2316 36-37 -0.7077318766111773 2316 38-39 -0.740726405272568 2316 40-41 -0.7808778180830487 2316 42-43 -0.6458572257514916 2316 44-45 -0.39373826902698283 2316 46-47 -0.6666271515671696 2316 48-49 -0.6846933695104198 2316 50-51 -0.5582098722857367 2316 52-53 -0.5627611737984068 2316 54-55 -0.47570743110458835 2316 56-57 0.28550036087465 2316 58-59 3.286732864442815 2316 60-61 3.390524869188134 2316 62-63 0.8753508893062332 2316 64-65 0.42542803536855445 2316 66-67 -1.3970031035217438 2316 68-69 -2.880945088609062 2316 70-71 -2.935319205035192 2316 72-73 -0.5957041717169993 2316 74-75 5.303404665902622 2316 76-77 5.661689167428232 2316 78-79 4.7894084694064425 2316 80-81 3.2654833517338204 2316 82-83 1.7033083315460296 2316 84-85 1.28178339311334 2316 86-87 1.1399054611321446 2316 88-89 1.0775941077555409 2316 90-91 2.5001208619370807 2316 92-93 4.195543364226001 2316 94-95 6.630214540549037 2316 96-97 3.5639107080431174 2316 98-99 5.987963636651889 2316 100-101 6.114632036424105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 21.0 4 48.0 5 162.0 6 335.0 7 745.0 8 1611.0 9 2768.0 10 4606.0 11 7236.0 12 10366.0 13 13688.0 14 17580.0 15 23394.0 16 30726.0 17 40371.0 18 40999.0 19 38086.0 20 38405.0 21 38627.0 22 39415.0 23 40030.0 24 41837.0 25 44026.0 26 53912.0 27 74921.0 28 104692.0 29 136567.0 30 163002.0 31 179853.0 32 184925.0 33 181926.0 34 184564.0 35 192017.0 36 185482.0 37 132299.0 38 46403.0 39 2295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.89732868635929 21.246499861365564 15.552824943229526 21.303346509045625 2 14.876311816346362 23.35441118035508 39.57804265445953 22.19123434883902 3 19.013891131234438 27.72360125869202 31.680795982142275 21.581711627931266 4 11.90090441755953 18.870877127935724 39.71678148674706 29.51143696775769 5 11.650103697265815 40.03324060160525 35.009659978341794 13.306995722787143 6 27.844990062851867 39.55711695198706 18.38879380384979 14.209099181311286 7 25.067244922959357 35.221456755019595 22.522447249561893 17.18885107245915 8 24.975260472196425 34.86097560338773 20.916367776036125 19.247396148379725 9 25.72561634470481 16.938670802539487 21.84031544733703 35.49539740541867 10-11 23.485852347077365 27.751732745329193 28.69760041915748 20.06481448843596 12-13 25.78364650472183 25.775291206091712 28.578080483284396 19.86298180590206 14-15 22.135494635185662 26.61734269361723 28.74076997792802 22.50639269326909 16-17 19.942274447685264 29.87555171637236 29.021787969020114 21.160385866922258 18-19 19.72742889532221 28.690246558591255 31.876458512162564 19.70586603392397 20-21 21.535999805043033 27.035135336255077 31.35708762140749 20.0717772372944 22-23 20.5311228346395 27.512431770500832 31.313222303599346 20.643223091260314 24-25 20.862284975985023 27.424550934945675 31.6992472826761 20.0139168063932 26-27 20.651861960742675 27.274574776849096 32.11606218698874 19.95750107541949 28-29 19.993615975466547 28.18677384168794 31.787807557562296 20.03180262528322 30-31 20.393367555543275 27.598222754512907 31.59870059758672 20.4097090923571 32-33 20.36900232503234 27.660073091096226 31.544563482522005 20.426361101349432 34-35 20.954486181226258 27.471069862938286 31.43596138485182 20.138482570983633 36-37 20.822593110302535 27.19797933913902 31.893655296493307 20.085772254065134 38-39 20.48364450978821 27.748089043267406 31.758855376982815 20.009411069961573 40-41 20.614014075919375 27.767189868125357 31.496372140828875 20.122423915126387 42-43 20.234559658404205 28.02673464436921 30.854268227087683 20.884437470138895 44-45 20.722514110599928 27.692882612962112 30.65644614539656 20.928157131041402 46-47 21.09349991311267 28.025867599388 30.259851912631852 20.62078057486748 48-49 20.996703812107274 27.962965462970907 30.18182815838947 20.85850256653235 50-51 20.439230970214247 28.49301079951228 30.382794809266677 20.684963421006795 52-53 20.929487779591565 28.823169290027167 29.483164091057727 20.76417883932354 54-55 20.483353709083058 28.130899389075815 29.929081071102658 21.456665830738473 56-57 21.074431630383707 28.402586018066067 29.479447592678635 21.043534758871584 58-59 21.098019103226683 27.6919418399239 30.665303402899234 20.544735653950177 60-61 20.59689455191863 28.20899540267578 30.77993385983761 20.414176185567978 62-63 20.875402925915594 28.340112773562055 29.903554538772493 20.880929761749858 64-65 20.608454487219657 28.776811389857887 29.70391713491947 20.91081698800298 66-67 21.596069469815358 27.592473259982036 29.820534916962227 20.990922353240386 68-69 21.7483060013564 26.812706143192504 31.142585174650744 20.296402680800355 70-71 22.79643432376677 26.76451800562225 30.177439382100367 20.26160828851061 72-73 21.925784932965357 26.793250113838685 30.634560649151943 20.646404304044015 74-75 21.233574254553535 27.95758205267701 30.0292782327075 20.779565460061953 76-77 19.799129134277393 28.091141433907463 30.362749641670018 21.746979790145122 78-79 20.88966533108979 27.923513878028984 29.806758242203134 21.38006254867809 80-81 20.623590982199005 28.512632265645117 29.274244683743323 21.589532068412556 82-83 20.59630838684325 28.504352923283193 29.324958000399054 21.574380689474502 84-85 20.473568121383 28.453601338429834 29.10815079660205 21.964679743585116 86-87 20.34523447653559 28.64244632039502 29.06722241648032 21.94509678658907 88-89 20.53042655975366 28.307077242559973 28.83091530120634 22.331580896480027 90-91 20.662703895119442 27.836650731743102 29.35980556715112 22.14083980598634 92-93 20.434924783171144 28.503692589175344 29.32785776706448 21.73352486058903 94-95 20.023981605607602 27.863633507637157 29.867446486264182 22.24493840049106 96-97 19.989582777862474 27.64902900559118 30.062514396145186 22.29887382040116 98-99 20.036005689950272 28.000797206273838 29.20532332299228 22.757873780783605 100-101 19.241154752898556 27.894733041243647 29.861710026761635 23.002402179096162 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2407.0 1 1760.5 2 1125.5 3 1136.5 4 1156.5 5 1205.0 6 1552.0 7 2384.0 8 3343.0 9 4060.5 10 4223.0 11 4241.0 12 4375.0 13 4454.0 14 4634.0 15 4925.5 16 5343.0 17 5775.5 18 6059.5 19 6420.5 20 7093.0 21 7905.5 22 8971.0 23 10423.5 24 12057.0 25 14274.0 26 17436.0 27 21187.5 28 25176.5 29 29791.5 30 34921.0 31 40501.0 32 46954.0 33 53746.5 34 60500.0 35 66495.5 36 71974.0 37 76298.5 38 78618.0 39 79660.5 40 80378.5 41 81214.0 42 82014.5 43 83286.5 44 84788.5 45 87069.0 46 90636.5 47 96613.0 48 102737.5 49 97778.5 50 84365.5 51 73614.5 52 65662.0 53 58986.5 54 52642.0 55 46133.0 56 40013.0 57 34390.5 58 29269.5 59 24512.5 60 19607.5 61 15027.0 62 11138.5 63 7913.0 64 5340.0 65 3492.0 66 2252.5 67 1460.5 68 892.5 69 491.5 70 296.5 71 205.0 72 134.5 73 83.0 74 51.5 75 33.5 76 23.0 77 13.5 78 6.0 79 2.5 80 2.0 81 2.5 82 1.5 83 0.5 84 1.5 85 1.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0232381743150287 2 0.011749638698610017 3 8.703436073044457E-5 4 0.0 5 0.058922262214510975 6 0.0 7 0.0 8 4.3517180365222287E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 3.0462026255655605E-4 16-17 2.1758590182611143E-5 18-19 4.5693039383483397E-4 20-21 0.0 22-23 0.0 24-25 8.703436073044457E-5 26-27 1.0879295091305572E-4 28-29 8.485850171218347E-4 30-31 0.005113268692913619 32-33 0.005461406135835397 34-35 0.0015883770833306134 36-37 0.08105074843022651 38-39 0.07445789560489532 40-41 0.07933181980580023 42-43 0.08250857397246146 44-45 0.0859029140409488 46-47 0.0812030585615048 48-49 0.07197741632407767 50-51 0.067147009303538 52-53 0.058051918607206536 54-55 0.047651312499918407 56-57 0.06936638550216433 58-59 0.7246916046220467 60-61 0.3397168685211078 62-63 0.0027850995433742263 64-65 0.0016971700342436693 66-67 1.0997879407800801 68-69 2.372056225937719 70-71 2.3422904745679074 72-73 1.8042440565322986 74-75 1.292677842748928 76-77 0.7495399146105887 78-79 0.5727948865572384 80-81 0.03953535836180445 82-83 1.958273116435003E-4 84-85 0.0011532052796783907 86-87 0.0011532052796783907 88-89 0.0 90-91 0.33921642094690774 92-93 0.05726860936063253 94-95 0.9587705178065774 96-97 1.663378943690074 98-99 0.6514739486575603 100-101 0.5719245429499339 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2297943.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.67278190181728 #Duplication Level Percentage of deduplicated Percentage of total 1 71.05701307819504 33.8748548707137 2 14.650136094091442 13.968254856911253 3 5.844493555098808 8.358697997364064 4 2.818040617912462 5.373753430728128 5 1.5683909628409103 3.738478015414796 6 0.9857901311226639 2.819721475318477 7 0.6532443313166435 2.179938217481977 8 0.4495783522296129 1.7146120586896578 9 0.3311351685972508 1.4207521205302407 >10 1.4953150177266665 12.462671590371935 >50 0.08998214266879324 2.9698496160273384 >100 0.05020945673888022 4.274595425832256 >500 0.0031984685086226005 0.9719503949418213 >1k 0.002924314065026076 3.0350033287143163 >5k 1.8276962906412975E-4 0.5157741248639747 >10k+ 3.655392581282595E-4 2.321092476096194 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16814 0.7316978706608476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13506 0.5877430380126922 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12771 0.5557579104442539 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10187 0.44330951638051946 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6563 0.28560325473695386 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5276 0.2295966436069128 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4318 0.18790718481702984 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3687 0.16044784400657458 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3532 0.15370268104996512 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3508 0.15265826872119978 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3312 0.1441289013696162 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3305 0.14382428110705966 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3296 0.14343262648377267 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3232 0.14064752694039842 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2886 0.12559058253403152 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2667 0.11606032003404784 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2654 0.11549459668929996 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2521 0.10970681170072538 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2477 0.10779205576465561 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2370 0.10313571746557683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.3517180365222287E-5 0.0 12-13 0.0 0.0 0.0 4.3517180365222287E-5 0.0 14-15 0.0 0.0 0.0 6.527577054783343E-5 0.0 16-17 0.0 0.0 0.0 1.3055154109566685E-4 0.0 18-19 0.0 0.0 0.0 2.1758590182611144E-4 0.0 20-21 0.0 0.0 0.0 3.6989603310438944E-4 0.0 22-23 0.0 0.0 0.0 0.0010008951484001126 0.0 24-25 0.0 0.0 0.0 0.0017624458047915026 0.0 26-27 0.0 0.0 0.0 0.002567513641548115 0.0 28-29 0.0 0.0 0.0 0.005483164726018008 0.0 30-31 0.0 0.0 0.0 0.011901948829888296 0.0 32-33 0.0 0.0 0.0 0.02567513641548115 0.0 34-35 0.0 0.0 0.0 0.04484445436636157 0.0 36-37 0.0 0.0 0.0 0.07430558547361706 0.0 38-39 0.0 0.0 0.0 0.11860607508541335 0.0 40-41 0.0 0.0 0.0 0.1696952448341843 0.0 42-43 0.0 0.0 0.0 0.22387413438888604 0.0 44-45 0.0 4.3517180365222287E-5 0.0 0.2904119031673109 0.0 46-47 0.0 4.3517180365222287E-5 0.0 0.3696784472025633 0.0 48-49 2.1758590182611143E-5 4.3517180365222287E-5 0.0 0.4489232326476331 0.0 50-51 4.3517180365222287E-5 4.3517180365222287E-5 0.0 0.5309531176360771 0.0 52-53 4.3517180365222287E-5 4.3517180365222287E-5 0.0 0.6130047612147038 0.0 54-55 4.3517180365222287E-5 6.527577054783343E-5 0.0 0.7055658038515316 0.0 56-57 6.527577054783343E-5 1.52310131278278E-4 0.0 0.7942755760260372 0.0 58-59 8.703436073044457E-5 1.7406872146088915E-4 0.0 0.8587245201469315 0.0 60-61 1.087929509130557E-4 1.7406872146088915E-4 0.0 0.9161236810486596 0.0 62-63 1.7406872146088915E-4 1.7406872146088915E-4 0.0 0.9674957124698045 0.0 64-65 1.7406872146088915E-4 2.3934449200872256E-4 0.0 1.0236546337311239 0.0 66-67 1.7406872146088915E-4 2.611030821913337E-4 0.0 1.0723503585598078 0.0 68-69 1.7406872146088915E-4 2.611030821913337E-4 0.0 1.1339706859569625 0.0 70-71 1.7406872146088915E-4 2.611030821913337E-4 0.0 1.2291862765960686 0.0 72-73 1.7406872146088915E-4 2.611030821913337E-4 0.0 1.359933645003379 0.0 74-75 1.7406872146088915E-4 2.611030821913337E-4 0.0 1.5393114624688253 0.0 76-77 1.7406872146088915E-4 2.611030821913337E-4 0.0 1.7649045254821378 0.0 78-79 1.7406872146088915E-4 2.611030821913337E-4 0.0 2.0519220885809615 0.0 80-81 1.7406872146088915E-4 2.611030821913337E-4 0.0 2.398014224025574 0.0 82-83 1.7406872146088915E-4 2.8286167237394485E-4 0.0 2.734097407986186 0.0 84-85 1.7406872146088915E-4 3.04620262556556E-4 0.0 3.05061961937263 2.1758590182611143E-5 86-87 1.7406872146088915E-4 3.04620262556556E-4 0.0 3.340313489063915 4.3517180365222287E-5 88-89 1.7406872146088915E-4 3.04620262556556E-4 4.3517180365222287E-5 3.623283954388773 4.3517180365222287E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12030 0.0 22.555113 1 TACATGG 11930 0.0 22.223667 2 GAGTACT 12360 0.0 22.105461 12-13 TACGCGG 125 7.2868716E-9 21.388111 64-65 AGTACTT 12855 0.0 21.36429 12-13 TTACGCG 115 7.31161E-8 21.070213 62-63 ACATGGG 11985 0.0 20.704182 3 GTACTTT 13705 0.0 19.884443 14-15 CGCGTGA 100 1.0535392E-5 19.444393 66-67 CATGGGA 9310 0.0 18.798994 4 GTATCAA 31525 0.0 18.246996 1 GCTTACG 380 0.0 18.242197 60-61 TACTTTT 15355 0.0 18.008718 14-15 TACGCGC 95 1.5649074E-4 17.908707 64-65 AGAGTAC 23190 0.0 17.901657 10-11 ACTTTTT 15680 0.0 17.86097 16-17 ATGGGAG 3545 0.0 16.634285 5 CATGGGG 3095 0.0 16.309233 4 TCAACGC 35825 0.0 16.025618 4 AACGCAG 35945 0.0 16.015547 6 >>END_MODULE