##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939557_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 675992 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.851776352382867 31.0 31.0 31.0 28.0 34.0 2 31.06098592882756 31.0 31.0 34.0 28.0 34.0 3 31.043833358974663 31.0 31.0 34.0 27.0 34.0 4 34.86504278157138 35.0 35.0 37.0 32.0 37.0 5 34.623622764766445 35.0 35.0 37.0 32.0 37.0 6 34.73755606575226 35.0 35.0 37.0 32.0 37.0 7 34.67320175386691 35.0 35.0 37.0 31.0 37.0 8 34.65581397412987 35.0 35.0 37.0 30.0 37.0 9 36.09806920791962 37.0 35.0 39.0 31.0 39.0 10-11 35.94557036177943 37.0 35.0 39.0 30.0 39.0 12-13 35.923885933561344 37.0 35.0 39.0 30.0 39.0 14-15 36.56914800766873 38.0 35.0 40.0 30.5 41.0 16-17 36.53789467922697 38.0 35.0 40.0 30.5 41.0 18-19 36.61503760399532 38.0 35.0 40.0 30.5 41.0 20-21 36.547241683333525 38.0 35.0 40.0 30.0 41.0 22-23 36.34019189576208 38.0 34.5 40.0 30.0 41.0 24-25 36.105850365093076 38.0 34.0 40.0 29.5 41.0 26-27 35.62715387164345 38.0 34.0 40.0 28.0 41.0 28-29 35.41340947821868 38.0 34.0 40.0 27.0 41.0 30-31 35.134614019100816 37.0 34.0 40.0 27.0 40.5 32-33 34.82566361732091 37.0 33.0 39.5 27.0 40.0 34-35 34.68038748979278 37.0 33.0 39.5 26.0 40.0 36-37 34.41485845986343 37.0 33.0 39.0 25.0 40.0 38-39 34.30020991372679 37.0 33.0 39.0 25.0 40.0 40-41 34.21246405282903 37.0 33.0 40.0 24.5 41.0 42-43 33.94920797879264 37.0 33.0 40.0 24.0 41.0 44-45 33.62557840921194 37.0 33.0 39.0 23.5 40.0 46-47 33.14675913324418 37.0 31.5 39.0 21.5 40.0 48-49 32.65511130309234 36.0 31.0 39.0 19.0 40.0 50-51 32.27813346903514 36.0 31.0 39.0 17.0 40.0 52-53 32.022430738825314 36.0 31.0 39.0 14.5 40.0 54-55 31.606488686256643 35.5 30.0 39.0 8.5 40.0 56-57 29.153620900839062 34.0 24.0 38.5 3.5 40.0 58-59 23.855291186877952 28.5 10.0 36.0 2.0 39.0 60-61 24.27067701984639 29.0 11.5 35.5 2.0 39.0 62-63 27.047729854791182 32.0 22.5 36.5 2.0 39.0 64-65 27.14817335116392 33.0 22.5 37.0 2.0 39.0 66-67 20.5726687593936 22.0 5.0 32.5 2.0 37.0 68-69 14.064407566953456 9.5 2.0 23.5 2.0 32.5 70-71 12.946514159930887 9.5 2.0 21.0 2.0 30.5 72-73 13.581056432620503 9.5 2.0 23.0 2.0 31.0 74-75 14.247331329364844 10.5 2.0 24.0 2.0 31.5 76-77 15.667121060604268 13.0 2.0 27.5 2.0 32.0 78-79 18.101837003988212 20.0 2.0 31.0 2.0 34.5 80-81 20.935591693392823 27.5 2.0 33.5 2.0 35.0 82-83 22.015614829761297 29.5 2.0 34.0 2.0 35.0 84-85 22.02993896377472 30.0 2.0 34.0 2.0 35.0 86-87 21.82466656410135 30.0 2.0 34.0 2.0 35.0 88-89 21.190903886436523 29.0 2.0 34.0 2.0 35.0 90-91 19.593707469910886 27.5 2.0 34.0 2.0 35.0 92-93 16.847334731771973 13.5 2.0 32.5 2.0 34.5 94-95 11.067216032142392 2.0 2.0 25.5 2.0 33.5 96-97 9.151146759133244 2.0 2.0 13.5 2.0 32.0 98-99 8.734364459934437 2.0 2.0 6.0 2.0 32.0 100-101 8.42500281068415 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42441218183931895 1101 2 0.4353969537060607 1101 3 0.4658460180662374 1101 4 0.3985978520549551 1101 5 0.37185122852605446 1101 6 0.37968599947515713 1101 7 0.17034664716543801 1101 8 0.4555836369423787 1101 9 0.7094651349588545 1101 10-11 0.5956030675271364 1101 12-13 0.427321951137273 1101 14-15 0.47752157775322246 1101 16-17 0.4425364328984003 1101 18-19 0.3605939857236322 1101 20-21 0.4562403976494238 1101 22-23 0.5445047937689864 1101 24-25 0.5354639762360733 1101 26-27 0.47518174844800143 1101 28-29 0.5421432279132361 1101 30-31 0.7619735627898834 1101 32-33 0.7277481628282843 1101 34-35 0.9546379427072864 1101 36-37 0.5623654035403476 1101 38-39 0.667304593617402 1101 40-41 0.7677281175550448 1101 42-43 0.6930609971819237 1101 44-45 0.9576272922114768 1101 46-47 1.1170222901010902 1101 48-49 0.9256124751126009 1101 50-51 1.0479597914766288 1101 52-53 1.1359093863594794 1101 54-55 1.2914603631716624 1101 56-57 -2.54601586784511 1101 58-59 -9.476582165307542 1101 60-61 -9.6986628938389 1101 62-63 -5.370606066982127 1101 64-65 -6.920554374802027 1101 66-67 -9.106293892965558 1101 68-69 -4.827555696533427 1101 70-71 -4.435689933913041 1101 72-73 -5.245745257158019 1101 74-75 -6.2745797521859945 1101 76-77 -7.454484074096841 1101 78-79 -10.035318154427436 1101 80-81 -8.525606543558515 1101 82-83 -6.233037214308236 1101 84-85 -5.462869255583055 1101 86-87 -5.100418494482096 1101 88-89 -4.892511814951444 1101 90-91 -9.274901156540091 1101 92-93 -12.934879719428125 1101 94-95 -8.032650602393028 1101 96-97 -6.3497512030047965 1101 98-99 -6.19359273821096 1101 100-101 -5.993283292533208 1102 1 0.4159283578064539 1102 2 0.25399503655440014 1102 3 0.29624014678928035 1102 4 0.24266452442743258 1102 5 0.14954936272667396 1102 6 0.18585897106022742 1102 7 0.4086383280966288 1102 8 0.4453345784007823 1102 9 0.30015111578733666 1102 10-11 0.3478422407554831 1102 12-13 0.44383705726531275 1102 14-15 0.5875459700846193 1102 16-17 0.38174924529141663 1102 18-19 0.3470048895236957 1102 20-21 0.29738106009379095 1102 22-23 0.4692469625467979 1102 24-25 0.3873693596664012 1102 26-27 0.5665256803206447 1102 28-29 0.22312469830009007 1102 30-31 0.5463528421462627 1102 32-33 0.6103151347557016 1102 34-35 0.25336911353918623 1102 36-37 0.3558415247317157 1102 38-39 0.2767234570203527 1102 40-41 0.4634862055386151 1102 42-43 0.7635002731148219 1102 44-45 0.9822250327113125 1102 46-47 0.9069583137231376 1102 48-49 0.8504231135925764 1102 50-51 0.98452796162384 1102 52-53 0.6442049187114094 1102 54-55 0.20941012352770016 1102 56-57 -3.3243740927680783 1102 58-59 -9.159017547026131 1102 60-61 -9.080283058166184 1102 62-63 -4.9829391720831175 1102 64-65 -6.187221398081725 1102 66-67 -7.173531140483533 1102 68-69 -4.060293842716241 1102 70-71 -4.165965310496405 1102 72-73 -4.739397901800263 1102 74-75 -5.355478844962439 1102 76-77 -7.000620885120464 1102 78-79 -9.024212154769783 1102 80-81 -6.446479104325377 1102 82-83 -4.515096012664962 1102 84-85 -4.052973243943207 1102 86-87 -3.942168965211298 1102 88-89 -3.652167112794647 1102 90-91 -8.634992948734546 1102 92-93 -11.502909717716381 1102 94-95 -7.967720997805558 1102 96-97 -6.712353907558032 1102 98-99 -6.45023964851565 1102 100-101 -6.179297286028587 1103 1 0.18295201509545578 1103 2 0.1487094440565322 1103 3 0.24463635309788856 1103 4 0.29053856534815026 1103 5 0.17733602086818934 1103 6 0.1369192864907447 1103 7 0.11318862647684824 1103 8 0.2998209381620782 1103 9 0.38196590692118093 1103 10-11 0.275231946637831 1103 12-13 0.25531137525337755 1103 14-15 0.415124827714628 1103 16-17 0.3871973484457172 1103 18-19 0.25770395175728567 1103 20-21 0.16903760314579586 1103 22-23 0.2468071714129536 1103 24-25 0.2464779487542117 1103 26-27 0.31057139643318266 1103 28-29 0.45683367101960215 1103 30-31 0.13601343464413418 1103 32-33 0.18015475538656034 1103 34-35 0.5361909379211127 1103 36-37 0.18741654604459512 1103 38-39 0.3595772507117374 1103 40-41 0.3194245047713551 1103 42-43 0.3069119312563444 1103 44-45 0.4438352202645959 1103 46-47 0.15420096078196366 1103 48-49 0.27885182203247183 1103 50-51 0.5104897050168518 1103 52-53 0.28812009347696943 1103 54-55 -0.13953488926002322 1103 56-57 -3.9640932956666326 1103 58-59 -10.245308041484785 1103 60-61 -10.465673083741633 1103 62-63 -6.44665400584271 1103 64-65 -7.683638171312758 1103 66-67 -7.657280074839882 1103 68-69 -4.807856182869694 1103 70-71 -4.326571128910727 1103 72-73 -5.280118809728652 1103 74-75 -5.497182809156813 1103 76-77 -6.986221375316541 1103 78-79 -9.408310087421105 1103 80-81 -7.32254474797414 1103 82-83 -5.830739661428815 1103 84-85 -5.390089612229648 1103 86-87 -5.119105772542927 1103 88-89 -5.177356264542301 1103 90-91 -7.002253711735397 1103 92-93 -10.027885740734284 1103 94-95 -7.186617523807263 1103 96-97 -6.1227865588794295 1103 98-99 -6.196176882104737 1103 100-101 -5.944549310751519 1104 1 0.3229237768674942 1104 2 0.20321166888661324 1104 3 0.3489649922842375 1104 4 0.26816802112048066 1104 5 0.3019570662938449 1104 6 0.18689239294030813 1104 7 0.1704566084826169 1104 8 0.3436390888637533 1104 9 0.588028568893705 1104 10-11 0.28857634299987467 1104 12-13 0.2717011605939419 1104 14-15 0.4831364775116995 1104 16-17 0.4018772540738098 1104 18-19 0.3513317083462013 1104 20-21 0.27498291144367926 1104 22-23 0.6573280505875587 1104 24-25 0.5817763449206623 1104 26-27 0.7816959746934131 1104 28-29 0.4867099606875982 1104 30-31 0.6085529803876213 1104 32-33 0.49314372903088355 1104 34-35 0.5925313980453168 1104 36-37 0.6777914054646672 1104 38-39 0.7162835242435932 1104 40-41 0.830949002350927 1104 42-43 0.8444070415199008 1104 44-45 0.5913814893956868 1104 46-47 0.5514018922767576 1104 48-49 0.5100156404276603 1104 50-51 0.23306592378366275 1104 52-53 0.06383857912559421 1104 54-55 0.10903944695825274 1104 56-57 -3.109718815201127 1104 58-59 -9.8825721117014 1104 60-61 -9.809390994846225 1104 62-63 -5.084058655982066 1104 64-65 -6.5759218615968535 1104 66-67 -6.9028762559557215 1104 68-69 -4.369718989089439 1104 70-71 -4.133056753085585 1104 72-73 -4.265458768654867 1104 74-75 -4.791850551565497 1104 76-77 -6.6255819200331825 1104 78-79 -8.670190618935457 1104 80-81 -5.958673482510349 1104 82-83 -4.085175569208388 1104 84-85 -3.577704215427538 1104 86-87 -3.2253689137096195 1104 88-89 -3.33068311897485 1104 90-91 -5.816666821118801 1104 92-93 -7.36201365623964 1104 94-95 -6.573938131601503 1104 96-97 -6.034490143056242 1104 98-99 -5.699401052071976 1104 100-101 -5.460257589075318 1105 1 0.3943985130100245 1105 2 0.31571768989765303 1105 3 0.34790712350874387 1105 4 0.46255064646421573 1105 5 0.35635347136917517 1105 6 0.3206289561776927 1105 7 0.4364633520149823 1105 8 0.39532527681685536 1105 9 0.5410189535677148 1105 10-11 0.3658358226625751 1105 12-13 0.41263854817633216 1105 14-15 0.41948295849750394 1105 16-17 0.5610057165362363 1105 18-19 0.2819635639151059 1105 20-21 0.37328696792838656 1105 22-23 0.4563360318568854 1105 24-25 0.638128435354723 1105 26-27 0.6396245030058836 1105 28-29 0.7604556087922774 1105 30-31 0.5865938196098597 1105 32-33 0.6709830549215354 1105 34-35 0.5088271501076989 1105 36-37 0.6616078423675233 1105 38-39 0.511502079322895 1105 40-41 0.4942860753164453 1105 42-43 0.6871824379920426 1105 44-45 0.7865994347523468 1105 46-47 0.6870240227695916 1105 48-49 0.7717101206086241 1105 50-51 0.5525773530344864 1105 52-53 0.4451011927626425 1105 54-55 0.2212131691320387 1105 56-57 1.3611410156618113 1105 58-59 -7.443337069721995 1105 60-61 -9.439623177226458 1105 62-63 -4.569218006993292 1105 64-65 -5.980684709977126 1105 66-67 -6.3914805398669685 1105 68-69 -3.9356742110589646 1105 70-71 -3.9375034602784815 1105 72-73 -2.774662531862983 1105 74-75 -4.268432629378301 1105 76-77 -6.063541117394303 1105 78-79 -8.370495175392723 1105 80-81 -5.404857838246922 1105 82-83 -3.4504466823790025 1105 84-85 -2.9442344058439183 1105 86-87 -2.6973064293337607 1105 88-89 -2.742852573090172 1105 90-91 -5.094087572419035 1105 92-93 -7.050070409222707 1105 94-95 -6.893894658377473 1105 96-97 -6.152617806782014 1105 98-99 -6.136259700073 1105 100-101 -5.978587375855311 1106 1 0.2482516705224782 1106 2 0.11268310883625077 1106 3 0.01027545392650353 1106 4 0.10582283805304371 1106 5 0.179294754152302 1106 6 0.3188045929145744 1106 7 0.2753686658566181 1106 8 -0.13402310154585706 1106 9 0.035080395394395225 1106 10-11 0.21401640393540333 1106 12-13 0.05400794593185054 1106 14-15 0.2028304434337187 1106 16-17 0.16101236608457725 1106 18-19 0.3252828138460515 1106 20-21 0.36497759001920826 1106 22-23 0.28895070246554155 1106 24-25 0.18466504367586367 1106 26-27 0.34935893027516585 1106 28-29 0.6214698183655756 1106 30-31 0.5126516945506125 1106 32-33 0.2667953739770965 1106 34-35 0.44551748234641764 1106 36-37 0.7299831019556606 1106 38-39 0.7552003535093448 1106 40-41 0.25203279913512233 1106 42-43 0.27932045533543004 1106 44-45 0.35371554268645156 1106 46-47 0.3162810124679112 1106 48-49 0.3823031921202542 1106 50-51 0.26687609848637095 1106 52-53 0.12867561484869583 1106 54-55 0.2467278271236033 1106 56-57 1.6337972203724185 1106 58-59 -7.148873756473709 1106 60-61 -8.337063271615946 1106 62-63 -4.305455999443499 1106 64-65 -5.824208049891805 1106 66-67 -7.023722153763503 1106 68-69 -4.112975040035991 1106 70-71 -3.8722321757562437 1106 72-73 -4.640202696839982 1106 74-75 -4.759837661892886 1106 76-77 -6.160553765176251 1106 78-79 -8.751983173810473 1106 80-81 -5.75777579646048 1106 82-83 -3.433519319317078 1106 84-85 -2.856091232543047 1106 86-87 -2.502407314917196 1106 88-89 -2.294844368570523 1106 90-91 -4.92701681781465 1106 92-93 -6.523660619723538 1106 94-95 -6.376145479340941 1106 96-97 -5.842595402919137 1106 98-99 -5.505600337679436 1106 100-101 -5.3560063621913425 1107 1 0.006192698803491936 1107 2 -0.10905413257831142 1107 3 0.11065370244600459 1107 4 0.12515513448889237 1107 5 -0.18567855669058275 1107 6 -0.24545931940187415 1107 7 0.10480268047540875 1107 8 0.027325814458144748 1107 9 0.12052585969810536 1107 10-11 0.03902352347799365 1107 12-13 -0.12038798826166186 1107 14-15 -0.024258320832750258 1107 16-17 -0.06248376745613626 1107 18-19 -0.04197331443715058 1107 20-21 0.019855024355116768 1107 22-23 -0.0526166557095209 1107 24-25 -0.09334214158558751 1107 26-27 -0.07202215145718327 1107 28-29 -0.07972571713356302 1107 30-31 -0.01705414055348342 1107 32-33 -0.13782466151108963 1107 34-35 0.11599858087358683 1107 36-37 0.15848920285601054 1107 38-39 0.10023529403378006 1107 40-41 0.04395869017326248 1107 42-43 0.13597850553003354 1107 44-45 -0.06128168251746047 1107 46-47 0.0847233660791602 1107 48-49 -0.07142343204822055 1107 50-51 -0.1310287479140655 1107 52-53 -0.5530762672662846 1107 54-55 -0.8848093733992286 1107 56-57 0.44155679159340266 1107 58-59 -8.633814098357796 1107 60-61 -10.201968125318473 1107 62-63 -5.573367125645607 1107 64-65 -6.324887144724112 1107 66-67 -7.446579171441874 1107 68-69 -4.464356413852139 1107 70-71 -4.322240208060711 1107 72-73 -3.2022969741881546 1107 74-75 -4.916762421106304 1107 76-77 -6.712090125958161 1107 78-79 -7.846461329593367 1107 80-81 -4.462420659071565 1107 82-83 -3.5062253661819227 1107 84-85 -3.155729048632484 1107 86-87 -3.0874675937111853 1107 88-89 -2.692440701458427 1107 90-91 -2.4353393805579024 1107 92-93 -4.855996304331452 1107 94-95 -5.544117395483683 1107 96-97 -5.318531348942051 1107 98-99 -5.2240808447369105 1107 100-101 -5.437453016736531 1108 1 -0.06482594224132754 1108 2 -0.0531597651656206 1108 3 -0.13300316424751202 1108 4 -0.10239937906038676 1108 5 -0.23227397697710472 1108 6 -0.2903862511089059 1108 7 -0.0616793976558796 1108 8 -0.019399057528502794 1108 9 -0.06533801464744471 1108 10-11 -0.06063990210165571 1108 12-13 -0.04966061354214446 1108 14-15 -0.3491029787925939 1108 16-17 -0.2648779047324723 1108 18-19 -0.1545926976717169 1108 20-21 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12.138479826127613 2316 98-99 11.270816130569946 2316 100-101 9.946497163086633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 36.0 5 101.0 6 258.0 7 447.0 8 793.0 9 1190.0 10 2028.0 11 3083.0 12 4350.0 13 5878.0 14 7946.0 15 10593.0 16 14557.0 17 19137.0 18 21206.0 19 21848.0 20 23184.0 21 23238.0 22 22087.0 23 19902.0 24 17429.0 25 17244.0 26 23462.0 27 35058.0 28 48852.0 29 59399.0 30 60149.0 31 54758.0 32 45991.0 33 37101.0 34 30103.0 35 22890.0 36 14069.0 37 6284.0 38 1297.0 39 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.81355450699786 24.13290542709984 17.157086864077627 17.89645320182467 2 15.712155372898506 27.733938113582703 36.96911596750046 19.584790546018333 3 22.530296216523272 27.974739346027768 32.62420265328584 16.870761784163125 4 12.37766127409792 20.70231600373969 40.80787938318797 26.112143338974427 5 10.01574028966279 42.68461523654116 35.68766408474584 11.611980389050206 6 25.000147930744742 43.05879359519047 17.389998698209446 14.551059775855336 7 23.07616066462325 37.196446111788305 23.19775973680162 16.529633486786828 8 23.51051129681488 34.37018759236494 20.9851668745109 21.13413423630928 9 23.561817299613015 20.038550752079907 23.519952898850875 32.87967904945621 10-11 22.737621155279943 28.1782920507935 29.552124877217484 19.531961916709072 12-13 27.268148143765014 26.772358252760387 28.1504810707819 17.809012532692694 14-15 21.920754686278414 29.05688623861761 30.357498156406276 18.664860918697702 16-17 17.914265257577014 31.330622255884684 31.149628989692186 19.605483496846116 18-19 17.694957928496194 32.13617912638019 32.61007526716293 17.558787677960687 20-21 20.004822542278607 28.71091669723902 31.834474372477782 19.449786388004593 22-23 19.01246767694101 29.131064456765504 31.72659869701829 20.129869169275196 24-25 20.584858992414112 29.790959064604316 32.19180108640339 17.432380856578185 26-27 20.43332066054329 27.461689465361122 34.73037283151057 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19.593676885191663 31.869377954661694 30.349663186145815 18.187281974000825 54-55 18.366641577243968 29.846051598359047 31.142338437809684 20.644968386587298 56-57 21.04710927622703 29.375897153887426 31.0043733112671 18.572620258618443 58-59 24.06596019036642 26.85164148158402 31.77638716064838 17.306011167401177 60-61 20.75045137331307 29.647582893118816 32.21670977681663 17.38525595675148 62-63 19.609685088177383 29.703151189333205 31.97996351892918 18.707200203560234 64-65 19.34117455356151 31.668283794893824 30.84354839904305 18.146993252501613 66-67 23.718820314162357 25.93884261851261 32.159236159003754 18.183100908321272 68-69 25.84999317672585 24.55014039172455 33.185051006833184 16.414815424716416 70-71 25.992181336939602 25.217929622894946 33.29038703448192 15.499502005683533 72-73 26.044367464062447 23.854963283095017 33.573575993880766 16.52709325896177 74-75 23.538641212055282 28.016465120776452 31.872050087326482 16.572843579841784 76-77 21.280880460721562 27.71555385963977 31.7873541935983 19.21621148604037 78-79 20.696349667876497 28.4452255891652 32.08771139293099 18.770713350027314 80-81 20.09452625486712 30.107041436278042 30.547182085898108 19.251250222956738 82-83 19.5368130173427 29.972832458444316 31.058498603160846 19.431855921052147 84-85 18.338678564243363 30.386454277565417 31.006432028781404 20.268435129409816 86-87 19.16772499021806 30.67343402015265 29.694056351383637 20.464784638245657 88-89 19.277535294452665 30.141873085682292 29.68062468241095 20.899966937454096 90-91 20.034255085411473 28.019591713206754 31.068650365953797 20.87750283542798 92-93 19.447463976752534 30.727181809198083 30.871624016340398 18.95373019770899 94-95 22.434964192814082 26.769677331131287 32.85572790316232 17.93963057289231 96-97 22.272110319628606 25.900015249673576 33.46537072259934 18.362503708098487 98-99 20.80162449422367 28.40693790651796 31.057859459914265 19.7335781393441 100-101 19.92444990789764 28.0890908207759 31.028669429114807 20.957789842211653 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2682.0 1 2116.5 2 1393.0 3 1172.0 4 1131.0 5 1185.5 6 1414.5 7 1878.5 8 2299.0 9 2532.5 10 2497.5 11 2348.0 12 2389.5 13 2439.0 14 2409.0 15 2401.5 16 2341.0 17 2265.5 18 2313.0 19 2407.5 20 2521.5 21 2682.0 22 2897.0 23 3341.5 24 3975.0 25 4696.0 26 5811.0 27 7130.0 28 8433.0 29 9744.0 30 11281.0 31 13269.0 32 15734.5 33 18354.5 34 20810.5 35 22842.5 36 24367.5 37 25351.0 38 25546.5 39 24927.5 40 24062.0 41 23550.0 42 23201.5 43 23530.5 44 24753.5 45 27088.5 46 30540.0 47 34069.0 48 35583.0 49 32180.5 50 25151.5 51 18733.5 52 13977.0 53 10369.0 54 7842.5 55 6113.5 56 4934.0 57 3963.5 58 3149.0 59 2553.5 60 1982.0 61 1462.0 62 1059.5 63 753.0 64 505.0 65 331.0 66 223.5 67 131.0 68 74.5 69 54.5 70 33.0 71 15.5 72 10.0 73 6.5 74 5.0 75 3.0 76 1.5 77 2.0 78 1.5 79 1.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02115409649818341 2 0.006508952768671819 3 0.0 4 0.0 5 0.09689463780636458 6 0.0 7 0.0 8 7.396537237127067E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.6982686185635334E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.917229789701654E-4 24-25 0.0 26-27 4.43792234227624E-4 28-29 0.002736718777737015 30-31 0.012574113303116013 32-33 0.0025887880329944733 34-35 0.00443792234227624 36-37 0.08261932093870933 38-39 0.08276725168345188 40-41 0.0923087847193458 42-43 0.09452774589048392 44-45 0.09193895785748944 46-47 0.08838861998366845 48-49 0.08809275849418337 50-51 0.08624362418490158 52-53 0.0696014154013657 54-55 0.060429709227328134 56-57 0.6028917501982272 58-59 1.6833039444253777 60-61 0.5734535319944615 62-63 0.003698268618563533 64-65 0.13713180037633582 66-67 1.9735440656102439 68-69 2.980582610445094 70-71 2.863643356726115 72-73 2.720890188049563 74-75 2.087006946827773 76-77 1.5296778660102488 78-79 1.0190209351589958 80-81 0.061021432206298305 82-83 2.21896117113812E-4 84-85 0.0 86-87 2.21896117113812E-4 88-89 7.396537237127067E-5 90-91 0.2857282334702186 92-93 0.4524461827950627 94-95 2.098175718055835 96-97 2.509127326950615 98-99 1.5430655984094486 100-101 1.0206481733511639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 675992.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.69512727231701 #Duplication Level Percentage of deduplicated Percentage of total 1 80.87376631502987 36.14663278441884 2 10.876011962523018 9.72209477760417 3 3.3228398420379417 4.455442489362347 4 1.4800897235577022 2.6461119427543998 5 0.762062679956201 1.7030244235062695 6 0.46071630912502054 1.235506444366496 7 0.3445513140696947 1.077983538393235 8 0.22416298603430718 0.8015194552436782 9 0.1798938311165112 0.7236339909531453 >10 1.2185001426303523 10.955909520033542 >50 0.14609467045860525 4.526976308931821 >100 0.0927585195506846 7.706105819355631 >500 0.006956888966301339 2.334856041222382 >1k 0.010600973662935375 11.77807461824311 >5k 6.625608539334609E-4 1.793674049297225 >10k+ 3.3128042696673046E-4 2.3924537963137325 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16158 2.390264973549983 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6529 0.9658398324240524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5585 0.8261932093870933 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4868 0.7201268654066912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4692 0.6940910543320039 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4601 0.6806293565604327 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4542 0.6719014426206227 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4394 0.6500076923987267 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4149 0.613764659936804 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3944 0.583438857264583 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3670 0.5429058332051268 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3414 0.5050355625510361 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3218 0.476041136581498 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3208 0.4745618291340726 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2947 0.4359519047562693 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2934 0.4340288050746163 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2886 0.42692812932697427 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2477 0.3664244547272749 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2436 0.3603592941928307 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1820 0.2692339554314252 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1774 0.2624291411732683 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1493 0.22086060190061424 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1473 0.21790198700576338 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1447 0.2140557876424573 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1443 0.21346406466348714 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1351 0.19985443614717333 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1285 0.19009100699416562 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1258 0.186096876886117 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1242 0.18372998497023632 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1232 0.1822506775228109 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1146 0.16952863347495237 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1108 0.1639072651747358 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1045 0.1545876282559557 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1036 0.15325625155327283 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1013 0.1498538444241944 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 995 0.14719109101882863 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 988 0.14615557580563082 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 967 0.14304903016603746 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 962 0.14230937644232478 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 882 0.13047491686292145 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 878 0.12988319388395128 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 873 0.12914354016023857 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 814 0.12041562622042863 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 787 0.11642149611238004 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 763 0.11287115823855903 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 740 0.10946875110948058 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 0.10754565142782756 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 724 0.10710185919359994 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.396537237127066E-5 0.0 16-17 0.0 0.0 0.0 1.4793074474254132E-4 0.0 18-19 0.0 0.0 0.0 2.21896117113812E-4 0.0 20-21 0.0 0.0 0.0 0.001331376702682872 0.0 22-23 0.0 0.0 0.0 0.0027367187777370145 0.0 24-25 0.0 0.0 0.0 0.0034763725014497213 0.0 26-27 0.0 0.0 0.0 0.004955679948875134 0.0 28-29 0.0 0.0 0.0 0.009097740801666292 0.0 30-31 0.0 0.0 0.0 0.017307897134877338 0.0 32-33 0.0 0.0 0.0 0.03750044379223423 0.0 34-35 0.0 0.0 0.0 0.06960141540136569 0.0 36-37 0.0 0.0 0.0 0.11864045728351814 0.0 38-39 0.0 0.0 0.0 0.18439567332157777 0.0 40-41 0.0 0.0 0.0 0.2540710540953147 0.0 42-43 0.0 0.0 0.0 0.32492988082699203 0.0 44-45 0.0 0.0 0.0 0.41686883868448144 0.0 46-47 0.0 0.0 0.0 0.521677771334572 0.0 48-49 0.0 0.0 0.0 0.6003029621652327 0.0 50-51 0.0 0.0 0.0 0.6775228109208393 0.0 52-53 0.0 0.0 0.0 0.744683369033953 0.0 54-55 0.0 0.0 0.0 0.8158380572551154 0.0 56-57 0.0 0.0 0.0 0.8801879312181209 0.0 58-59 0.0 0.0 0.0 0.9141380371365342 0.0 60-61 0.0 0.0 0.0 0.9452774589048392 0.0 62-63 1.4793074474254132E-4 0.0 0.0 0.9714612007242689 0.0 64-65 1.4793074474254132E-4 0.0 0.0 0.9915797820092546 0.0 66-67 1.4793074474254132E-4 0.0 0.0 1.0104409519639286 0.0 68-69 1.4793074474254132E-4 0.0 0.0 1.0401010662848083 0.0 70-71 1.4793074474254132E-4 0.0 0.0 1.0890661427945894 0.0 72-73 1.4793074474254132E-4 0.0 0.0 1.1625877229316324 0.0 74-75 1.4793074474254132E-4 0.0 0.0 1.282485591545462 0.0 76-77 1.4793074474254132E-4 0.0 0.0 1.4532716363507259 0.0 78-79 1.4793074474254132E-4 0.0 0.0 1.7850802968082462 0.0 80-81 1.4793074474254132E-4 0.0 0.0 2.187599853252701 0.0 82-83 1.4793074474254132E-4 0.0 0.0 2.511124392004639 0.0 84-85 1.4793074474254132E-4 0.0 0.0 2.778287317009669 0.0 86-87 1.4793074474254132E-4 0.0 0.0 2.976218653475189 0.0 88-89 1.4793074474254132E-4 0.0 0.0 3.1466348714185965 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGGG 65 2.4638575E-7 29.937714 66-67 ATACCGG 50 4.217591E-5 29.18033 62-63 GCCGCGG 50 5.2932475E-5 28.224146 40-41 CAACGGG 70 4.725316E-7 27.790792 62-63 ACGTTTG 75 8.5998727E-7 25.949001 64-65 GTACATG 2595 0.0 25.746433 1 CGGCCGC 55 1.01255806E-4 25.658318 38-39 TACATGG 2620 0.0 24.77876 2 ACGGCGG 60 1.4538466E-4 24.327187 64-65 CATACGG 50 0.0014415239 24.316942 62-63 ACGCAGT 520 0.0 23.647442 88-89 ACATGGG 2620 0.0 23.518383 3 TAGGACG 120 1.8272435E-4 23.518381 4 CGCAGTC 125 3.1650416E-10 23.146673 88-89 GCGCGGA 75 2.5230765E-5 22.701897 68-69 CAACGTG 75 2.5283074E-5 22.695814 62-63 GCTTACG 225 0.0 22.434734 60-61 TCAACTC 1435 0.0 22.029287 62-63 CATGGGA 2100 0.0 21.726505 4 CGCGGTA 65 3.141814E-4 21.710882 42-43 >>END_MODULE