FastQCFastQC Report
Fri 27 May 2016
SRR939554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125631
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52224.156617395388081No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44673.5556510733815694No Hit
NCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35032.788324537733521No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29982.3863536865900934No Hit
NAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26972.146763139670941No Hit
NTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24481.9485636506913102No Hit
NCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23001.830758331940365No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21221.68907355668585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20391.6230070603593063No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18611.481322285104791No Hit
NTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17241.3722727670718213No Hit
NTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15291.2170562997986165No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14781.1764612237425476No Hit
NCGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14641.165317477374215No Hit
NATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14631.1645214954907626No Hit
NGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13551.0785554520779106No Hit
NGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13161.0475121586232696No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13101.0427362673225558No Hit
NTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12741.0140809195182718No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12150.9671179883945842No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12110.9639340608607747No Hit
NCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12000.955178260142799No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11620.9249309485716105No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10980.8739881080306612No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10310.820657321839355No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG10160.80871759358757No Hit
NCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC9760.7768783182494766No Hit
NTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA9200.732303332776146No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC8900.7084238762725761No Hit
NATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA8330.663052908915793No Hit
NAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC8100.6447453255963894No Hit
NTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG7850.6248457785100812No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7690.6121100683748438No Hit
NGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT7560.6017623038899635No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA7540.6001703401230588No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC7080.5635551734842514No Hit
NACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC6980.5555953546497282No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT6800.5412676807475862No Hit
NTACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC6790.5404716988641338No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG6390.5086324235260405No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC5970.4752011844210426No Hit
NCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG5760.4584855648685436No Hit
NTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.4322181627146166No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC4980.39639897795926166No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG4880.3884391591247383No Hit
NGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.38207130405711964No Hit
NGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.37888737652331034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.3669476482715253No Hit
NCTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG4340.3454561374183123No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG4120.327944535982361No Hit
NAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT4110.3271485540989087No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3830.3048610613622434No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT3820.3040650794787911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.3032690975953387No Hit
NATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.28655347804283976No Hit
NCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3300.2626740215392698No Hit
NTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT3240.2578981302385558No Hit
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2930.23322269185153346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.2316307280846288No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT2840.22605885490046249No Hit
NTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2740.21809903606593914No Hit
NACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT2660.21173118099832047No Hit
NACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG2620.20854725346451114No Hit
NGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG2530.20138341651344016No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2470.19660752521272618No Hit
NAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2300.1830758331940365No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG2150.1711361049422515No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT2120.16874815929189452No Hit
NTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.16078834045737117No Hit
NGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA1960.15601244915665718No Hit
NTCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA1930.1536245035063002No Hit
NTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.1512365578559432No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA1770.14088879337106286No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA1750.1392968296041582No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1750.1392968296041582No Hit
NCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.13850084772070587No Hit
NTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA1690.1345209383034442No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG1660.13213299265308723No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.12815308323582555No Hit
NAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA1610.12815308323582555No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.1241731738185639No Hit
NAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC1420.11302942745023124No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA1410.11223344556677892No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA1370.10904951803296957No Hit
NAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.10904951803296957No Hit
NGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT1310.10427362673225557No Hit
NCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1270.10108969919844625No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.1002937173149939No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA201.7870987E-594.4657064
GAGTATA400.00164726975.420211
ACCCATG352.856827E-453.9804043
GCTTTGC1759.458745E-1151.716711
CGCAGAA2650.044.523799
CTTTGCG2450.038.5574342
TTTGCGT2450.038.5574343
GCTTGAT1552.6422297E-432.4387971
TTGCGTT2950.032.0222744
CGCCGTA452.4796576E-531.52628194-95
CAGAAAA2350.030.12460710-11
TGCTTTG1652.7848728E-928.603167
GTTGATC853.787136E-927.81730794-95
TGCATAC350.00857952727.01174578-79
AGTACTT5200.026.3204112-13
GCAGAAA2400.023.59760910-11
TACTTTT5950.023.3993114-15
TGGTCGC653.0139112E-421.83460290-91
GAGAAAA1059.097912E-720.22652212-13
GAGTACG858.172195E-519.4798992-93
Produced by FastQC (version 0.11.5)