##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939554_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 125631 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.241620300721955 2.0 2.0 31.0 2.0 33.0 2 22.36998033924748 16.0 16.0 31.0 16.0 34.0 3 26.722401318145998 28.0 25.0 31.0 16.0 34.0 4 31.708089563881526 35.0 32.0 35.0 19.0 37.0 5 32.493468968646276 35.0 33.0 35.0 26.0 37.0 6 33.06697391567368 35.0 33.0 37.0 28.0 37.0 7 33.3364376626788 35.0 33.0 37.0 28.0 37.0 8 33.31695998599072 35.0 33.0 37.0 27.0 37.0 9 34.42873972188393 37.0 34.0 39.0 27.0 39.0 10-11 34.291854717386634 37.0 33.5 39.0 26.0 39.0 12-13 34.515748501564104 37.0 34.0 39.0 27.0 39.0 14-15 34.72334853658731 38.0 33.0 40.0 25.0 41.0 16-17 34.632049414555325 38.0 33.0 40.0 25.5 41.0 18-19 34.93510359704213 38.0 34.0 40.0 27.0 41.0 20-21 34.83828434064841 38.0 33.5 40.0 26.5 41.0 22-23 34.726643901584794 38.0 33.5 40.0 25.5 41.0 24-25 34.285049072283115 37.5 33.0 40.0 23.5 41.0 26-27 34.15298771799954 37.5 33.0 40.0 23.5 41.0 28-29 34.07387507860321 37.0 33.0 40.0 24.0 41.0 30-31 34.139201311778145 37.0 33.0 40.0 24.0 41.0 32-33 33.724271875572114 37.0 33.0 40.0 22.0 41.0 34-35 33.29876782004442 36.0 32.5 40.0 20.5 41.0 36-37 32.87612929929715 35.5 31.5 39.5 17.5 41.0 38-39 32.62906448249238 35.0 31.0 39.0 17.5 40.5 40-41 32.71089937993011 36.0 31.0 39.0 16.5 41.0 42-43 32.30215074304909 35.0 31.0 39.0 15.0 41.0 44-45 31.94118091872229 35.0 30.5 39.0 14.5 40.0 46-47 31.656478098558477 35.0 30.0 39.0 11.0 40.0 48-49 31.116734723117702 35.0 29.0 39.0 8.0 40.0 50-51 30.33592425436397 34.5 28.0 38.0 7.5 39.5 52-53 30.68301215464336 35.0 28.0 38.0 5.5 40.0 54-55 30.935226974234066 35.0 28.5 39.0 2.0 40.0 56-57 30.36766801187605 35.0 28.0 38.5 2.0 40.0 58-59 29.939982965987696 35.0 27.5 38.0 2.0 40.0 60-61 28.94707914447867 34.0 26.0 38.0 2.0 40.0 62-63 28.849782298954874 34.0 26.0 38.0 2.0 39.0 64-65 28.32922606681472 33.5 26.0 36.5 2.0 39.0 66-67 27.309167323351723 32.5 23.0 36.0 2.0 39.0 68-69 26.928731762065095 32.5 23.0 36.0 2.0 37.5 70-71 25.991156641274845 31.5 21.5 35.0 2.0 37.0 72-73 25.21814679497895 31.0 20.0 34.5 2.0 36.0 74-75 24.883519991085002 31.0 19.0 34.0 2.0 36.0 76-77 24.386091012568553 31.0 16.0 34.0 2.0 35.0 78-79 23.59289904561772 30.5 9.0 34.0 2.0 35.0 80-81 22.550914981175026 29.0 2.0 34.0 2.0 35.0 82-83 21.698199488979633 28.5 2.0 33.0 2.0 35.0 84-85 20.954605153186712 27.0 2.0 33.0 2.0 34.0 86-87 20.674940898345156 27.0 2.0 33.0 2.0 34.5 88-89 19.720578519632895 25.5 2.0 33.0 2.0 34.0 90-91 18.504537096735678 24.5 2.0 32.0 2.0 34.0 92-93 17.599262920775924 22.0 2.0 32.0 2.0 34.0 94-95 16.550158798385752 18.5 2.0 31.0 2.0 34.0 96-97 15.442832581130453 4.5 2.0 31.0 2.0 34.0 98-99 14.753432671872389 2.0 2.0 31.0 2.0 34.0 100-101 13.066965955854844 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 15.040537740995262 1101 2 7.856581636948338 1101 3 4.015391213464557 1101 4 2.5433868726918973 1101 5 0.9352105012025547 1101 6 1.000039595036867 1101 7 0.6592131969916366 1101 8 0.7751241829057491 1101 9 0.7859898581284526 1101 10-11 0.6294135292273566 1101 12-13 0.892107788869744 1101 14-15 0.34973071124197475 1101 16-17 0.3888359435602311 1101 18-19 1.624269473119405 1101 20-21 1.2507243831806178 1101 22-23 1.4450911800298414 1101 24-25 1.275810271710938 1101 26-27 1.2067382534236444 1101 28-29 0.9806718666300327 1101 30-31 1.3417301353417201 1101 32-33 1.6668504926072458 1101 34-35 1.760227071875299 1101 36-37 1.296493655521381 1101 38-39 1.0242826175985371 1101 40-41 1.3655340582579356 1101 42-43 1.4045331293174854 1101 44-45 1.0484277414245255 1101 46-47 1.069118923981545 1101 48-49 1.0120031897724893 1101 50-51 0.6073446624090089 1101 52-53 0.554694329183171 1101 54-55 0.8266137952689689 1101 56-57 1.148762111965116 1101 58-59 1.0285450649101868 1101 60-61 1.047238663959849 1101 62-63 1.0398240676618613 1101 64-65 1.083175068550215 1101 66-67 0.2793173374743425 1101 68-69 0.6119026458519023 1101 70-71 0.22687602710831456 1101 72-73 0.06737365967319775 1101 74-75 0.12190399999947488 1101 76-77 -0.1728543891052432 1101 78-79 0.10525317567514136 1101 80-81 -0.050887341321601554 1101 82-83 0.195781254905377 1101 84-85 0.6561404469829917 1101 86-87 0.6348057395363611 1101 88-89 1.2013071164445037 1101 90-91 1.7499498274087237 1101 92-93 2.6638012295295113 1101 94-95 2.4831199557581733 1101 96-97 1.8359836176242386 1101 98-99 1.0310303859804542 1101 100-101 -0.18637219501478341 1102 1 14.731143801601323 1102 2 8.26530890967561 1102 3 4.254966971040314 1102 4 2.350295963600992 1102 5 2.2083014102934584 1102 6 1.322221413218685 1102 7 1.1051525909310271 1102 8 1.0202756980572616 1102 9 1.376898949037546 1102 10-11 1.3965650443788675 1102 12-13 1.5135926373545843 1102 14-15 1.1479125294237917 1102 16-17 0.9623813981056841 1102 18-19 0.791481594331529 1102 20-21 0.9569062013624361 1102 22-23 1.2407881497268107 1102 24-25 1.1373254232260805 1102 26-27 1.9309200716054633 1102 28-29 2.050217321175488 1102 30-31 1.8760937717053565 1102 32-33 1.8308807956375475 1102 34-35 2.049802829451046 1102 36-37 2.4692815343092533 1102 38-39 2.401676556992477 1102 40-41 2.626473452197331 1102 42-43 1.899411917196268 1102 44-45 2.065973195969981 1102 46-47 1.8103310451936743 1102 48-49 2.03951834128765 1102 50-51 1.7711931472574953 1102 52-53 1.1026640261528655 1102 54-55 0.9017653104204868 1102 56-57 0.9642166574196622 1102 58-59 0.16566627703139858 1102 60-61 1.1359659366871213 1102 62-63 0.5044907343285274 1102 64-65 0.4200538564290035 1102 66-67 -0.37613720798020367 1102 68-69 0.07326628221553833 1102 70-71 0.7634517846840723 1102 72-73 0.029494871794408795 1102 74-75 -0.44724751515204275 1102 76-77 0.28753955028869527 1102 78-79 -0.4154437940218294 1102 80-81 -0.26179643223069604 1102 82-83 -1.5812793511552279 1102 84-85 -1.2208292499867035 1102 86-87 -1.3564366847060647 1102 88-89 -1.4817231865857998 1102 90-91 -1.0068986574397591 1102 92-93 0.5827103204386042 1102 94-95 0.5421502587884781 1102 96-97 -0.798955776315152 1102 98-99 -1.6177877958377263 1102 100-101 -1.4492206798632665 1103 1 13.494000944458467 1103 2 7.09756287792958 1103 3 2.8086812567546 1103 4 1.1025499318549592 1103 5 0.5109680769601255 1103 6 -0.06273096773369247 1103 7 0.0214065591849959 1103 8 -0.3168671590855894 1103 9 -0.48024390810530804 1103 10-11 -0.4744825746687482 1103 12-13 -0.8566295848676333 1103 14-15 -0.4947858832746235 1103 16-17 -1.0395551098308218 1103 18-19 -0.42515332630339486 1103 20-21 -0.17712554466931607 1103 22-23 -0.22806899313032858 1103 24-25 0.2725635184641817 1103 26-27 0.20745975414514106 1103 28-29 -0.06279855184038752 1103 30-31 0.29158583519742365 1103 32-33 0.03535698611373306 1103 34-35 -0.6369590753108554 1103 36-37 0.3816307406584585 1103 38-39 0.3327241760400952 1103 40-41 0.7297115474354214 1103 42-43 0.6428087425930968 1103 44-45 0.22717954517632677 1103 46-47 -0.051732446869824145 1103 48-49 -0.050767372998070215 1103 50-51 -0.5328068527425067 1103 52-53 0.19412434361318276 1103 54-55 -0.7217267530715787 1103 56-57 -1.4684817552787486 1103 58-59 -1.0351273737622542 1103 60-61 -1.398288031566846 1103 62-63 -0.9482711704333795 1103 64-65 -0.753946143570996 1103 66-67 -0.18280387464686854 1103 68-69 -0.37276546381621145 1103 70-71 -1.0730878978556113 1103 72-73 -1.137171794872259 1103 74-75 -1.611501483406009 1103 76-77 -2.3425874338382897 1103 78-79 -2.600554905132938 1103 80-81 -2.491002781437043 1103 82-83 -2.515438081313956 1103 84-85 -1.6162260753835298 1103 86-87 -2.2160239862933686 1103 88-89 -2.3279136627762753 1103 90-91 -1.9293431018842035 1103 92-93 -1.5429087271804462 1103 94-95 -2.2773418047035854 1103 96-97 -1.7623208556802314 1103 98-99 -1.410708430758362 1103 100-101 -1.5521730608156474 1104 1 14.588370078973586 1104 2 8.078214019164662 1104 3 4.044864781259292 1104 4 2.3678726059367605 1104 5 1.716564184016086 1104 6 1.4459586394960624 1104 7 1.5823496712229996 1104 8 1.6497647491521548 1104 9 1.1141252264098114 1104 10-11 1.126316869196394 1104 12-13 1.0918700096173595 1104 14-15 1.1204672739493304 1104 16-17 0.9629069455509409 1104 18-19 1.2363575067402905 1104 20-21 1.5652565663259352 1104 22-23 1.5151531132304612 1104 24-25 1.2576173940290047 1104 26-27 1.1909784657660438 1104 28-29 1.1569326496426413 1104 30-31 1.6259039906834616 1104 32-33 1.737216562060901 1104 34-35 1.7769269170422959 1104 36-37 1.9033837240902827 1104 38-39 1.8085670679413823 1104 40-41 1.9264442551170333 1104 42-43 1.881280530334955 1104 44-45 1.7538856047290992 1104 46-47 2.905659512346954 1104 48-49 2.1197227208496834 1104 50-51 1.7007697895932559 1104 52-53 1.480678624693013 1104 54-55 2.6045390330482263 1104 56-57 2.608450234061994 1104 58-59 3.1065421894401517 1104 60-61 2.728725060774714 1104 62-63 2.372549128489112 1104 64-65 2.1310903527793705 1104 66-67 2.58970220807819 1104 68-69 2.7017334355002056 1104 70-71 2.2499627335891823 1104 72-73 2.175480273254262 1104 74-75 2.1158765724391984 1104 76-77 2.0630723970040243 1104 78-79 1.7386583957591917 1104 80-81 1.0881305750685755 1104 82-83 1.7694651743922165 1104 84-85 1.7000466624220572 1104 86-87 1.5435925123742287 1104 88-89 0.8982768134141992 1104 90-91 1.3891889338011154 1104 92-93 1.63792199927072 1104 94-95 1.1803108427300835 1104 96-97 0.6236135667505405 1104 98-99 0.0691027151111765 1104 100-101 0.3816990281659294 1105 1 14.487507437964961 1105 2 7.985369515736217 1105 3 4.409330607403952 1105 4 2.7479323272373577 1105 5 1.4049074708995235 1105 6 1.7424638374611092 1105 7 1.6364859242643632 1105 8 1.388760546542116 1105 9 1.596595918734515 1105 10-11 1.4665347413485676 1105 12-13 1.5019562737182284 1105 14-15 1.0883670748783345 1105 16-17 1.1047450344693246 1105 18-19 1.5257846246345608 1105 20-21 1.3416334740897042 1105 22-23 1.5019093618480213 1105 24-25 2.0902042111048758 1105 26-27 2.1120412837266684 1105 28-29 2.1700658060239704 1105 30-31 1.618245286856876 1105 32-33 1.9206383713951212 1105 34-35 1.741287677935901 1105 36-37 2.1298269888547097 1105 38-39 2.2704947388106618 1105 40-41 2.592806785530655 1105 42-43 2.4424119171962744 1105 44-45 2.5560034990002833 1105 46-47 2.932755287617912 1105 48-49 2.894578947348254 1105 50-51 2.4103749654393063 1105 52-53 2.884239783728624 1105 54-55 3.0349471286023046 1105 56-57 3.0123984756014757 1105 58-59 2.895969307334429 1105 60-61 2.9070871488083334 1105 62-63 2.2064907343285256 1105 64-65 2.9240841594593086 1105 66-67 3.605074913231917 1105 68-69 2.634629918579172 1105 70-71 2.067785118017408 1105 72-73 2.11661608391562 1105 74-75 2.057510060605537 1105 76-77 2.240024398773542 1105 78-79 2.241616812038778 1105 80-81 1.9983550829208205 1105 82-83 1.5821448912690137 1105 84-85 1.8720495378920852 1105 86-87 1.7370784668090877 1105 88-89 1.5005495406869258 1105 90-91 2.571919524378419 1105 92-93 2.004710320438601 1105 94-95 0.31645328909150905 1105 96-97 0.01022604186666598 1105 98-99 -0.5409393109892431 1105 100-101 -7.661344087228628E-4 1106 1 14.93085394652886 1106 2 7.832207460400248 1106 3 3.979488710170749 1106 4 2.7792234998328738 1106 5 1.7863303958007108 1106 6 1.3616996740882499 1106 7 1.4806888228150825 1106 8 1.1779568574775539 1106 9 0.7319714128056631 1106 10-11 1.0757244646687312 1106 12-13 0.7315346663400959 1106 14-15 0.9070429642063971 1106 16-17 1.1893958908593092 1106 18-19 0.980988840708342 1106 20-21 1.1486163462899626 1106 22-23 1.2517446714659428 1106 24-25 1.738919626124634 1106 26-27 1.1517316658083558 1106 28-29 1.9587680458131658 1106 30-31 1.673745945618407 1106 32-33 2.4064749985360905 1106 34-35 2.4598318149582994 1106 36-37 1.9107887806860688 1106 38-39 1.7535316294562406 1106 40-41 2.1091111333567483 1106 42-43 1.5166872795151178 1106 44-45 1.1171036307525881 1106 46-47 1.6577223495414977 1106 48-49 1.6272864572296761 1106 50-51 1.6773380747937274 1106 52-53 2.2715915623847494 1106 54-55 2.149736324913235 1106 56-57 2.0492891211877726 1106 58-59 1.644651784277773 1106 60-61 1.6061978207450913 1106 62-63 1.3043168212850489 1106 64-65 1.7206915375884257 1106 66-67 1.4113990239038543 1106 68-69 1.083411209751766 1106 70-71 1.620321349901463 1106 72-73 1.3519586399103503 1106 74-75 0.9908104558624515 1106 76-77 1.3879163618828976 1106 78-79 1.6808460610506337 1106 80-81 1.195160089508434 1106 82-83 1.1821119531925994 1106 84-85 1.0497504601582257 1106 86-87 1.1870125906562556 1106 88-89 0.6845086974721681 1106 90-91 -0.1370725704832374 1106 92-93 1.2547103204386012 1106 94-95 1.474556055889927 1106 96-97 1.6141456729602073 1106 98-99 0.6698643780753155 1106 100-101 1.281677870861369 1107 1 14.423607569717266 1107 2 7.520613257501697 1107 3 3.2331118985765457 1107 4 1.945890166499538 1107 5 0.9964753233369379 1107 6 1.1153228624940468 1107 7 0.44445693875711356 1107 8 0.4098409154485694 1107 9 0.48559460121146003 1107 10-11 0.6010867835093094 1107 12-13 0.3148679996734316 1107 14-15 0.3164632540614676 1107 16-17 0.37055531114916107 1107 18-19 1.0353366667952955 1107 20-21 0.9276018535363448 1107 22-23 0.37855626566884126 1107 24-25 0.8693544087333365 1107 26-27 0.32564470928661393 1107 28-29 0.9986231182769387 1107 30-31 0.9527314528647821 1107 32-33 0.852127172449137 1107 34-35 0.6192521048133699 1107 36-37 0.6100641430049123 1107 38-39 0.8006330787316074 1107 40-41 0.9243285246610924 1107 42-43 0.8174119171962744 1107 44-45 1.0265239206076586 1107 46-47 -0.019813882342564426 1107 48-49 0.7432284862151768 1107 50-51 0.6229902487067669 1107 52-53 0.5976785189064913 1107 54-55 1.2294464698407737 1107 56-57 0.2992891211877797 1107 58-59 0.07581120456763202 1107 60-61 -0.16554130968969005 1107 62-63 0.7028675459227287 1107 64-65 0.9815611028058164 1107 66-67 0.7882106181067527 1107 68-69 0.3261648329401723 1107 70-71 -0.8543163312579587 1107 72-73 -0.41978049052443467 1107 74-75 -0.0997692542824744 1107 76-77 -0.25701117434898535 1107 78-79 -0.507559736050812 1107 80-81 -0.33744860614373806 1107 82-83 -0.07875761202479481 1107 84-85 -0.6857567862185867 1107 86-87 -0.1173352354307049 1107 88-89 -0.18143333151333607 1107 90-91 -0.6769276429470068 1107 92-93 -0.056883882459949575 1107 94-95 0.007164751542102721 1107 96-97 -0.12136157341660159 1107 98-99 -0.9968022885913506 1107 100-101 -0.7944090856603694 1108 1 14.376691246856797 1108 2 7.589162924274152 1108 3 3.1616531024271772 1108 4 -0.14307629917273346 1108 5 0.3370021402204699 1108 6 0.43865936942306405 1108 7 0.45826207998212 1108 8 0.10231949367770454 1108 9 0.20901573735871182 1108 10-11 -0.11090940817587125 1108 12-13 0.037125484069918 1108 14-15 0.4562336973069989 1108 16-17 0.3278704492005815 1108 18-19 0.11591955053590652 1108 20-21 -0.05883102491494441 1108 22-23 -1.094335937864436 1108 24-25 0.3087122845399577 1108 26-27 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5 -0.6556985897065388 2312 6 -0.25122303122575573 2312 7 -0.09962518684675103 2312 8 -0.3763909686093996 2312 9 0.8421767268153246 2312 10-11 0.5091483777122008 2312 12-13 -0.7420462515342976 2312 14-15 0.6505514183126735 2312 16-17 0.07602028699457009 2312 18-19 0.05103714988708674 2312 20-21 -0.063038243082012 2312 22-23 0.6022881497268102 2312 24-25 0.35788097878164393 2312 26-27 -0.204246595061214 2312 28-29 -0.4432549010467355 2312 30-31 0.16559377170536038 2312 32-33 0.45946412897087185 2312 34-35 -1.089836059437836 2312 36-37 0.02597597875369928 2312 38-39 0.10950989032581049 2312 40-41 0.16919567441954797 2312 42-43 -0.4082825272481685 2312 44-45 -0.1220268040300212 2312 46-47 0.7458866007492269 2312 48-49 0.8926850079543165 2312 50-51 1.3491375917019361 2312 52-53 1.2308306928195378 2312 54-55 0.532737532642706 2312 56-57 0.07268887964188409 2312 58-59 -0.36350038963526643 2312 60-61 0.16024371446490093 2312 62-63 0.5606574009951899 2312 64-65 0.7564705230956719 2312 66-67 0.30677945868646006 2312 68-69 0.9079885044377569 2312 70-71 1.5573684513507402 2312 72-73 1.0260226495721874 2312 74-75 1.3011969292924022 2312 76-77 0.8984839947331373 2312 78-79 0.6251395393115082 2312 80-81 0.858203567769305 2312 82-83 1.3779150932892144 2312 84-85 0.7855596389021855 2312 86-87 1.7800077597383783 2312 88-89 2.804526813414199 2312 90-91 1.7442680092269036 2312 92-93 2.2685992093274905 2312 94-95 3.216705814344035 2312 96-97 2.4663497792404048 2312 98-99 2.321434426384494 2312 100-101 1.8982237645811768 2313 1 -14.148856198398676 2313 2 -7.081958695958191 2313 3 -2.977033028959685 2313 4 -1.168520937807461 2313 5 0.09782253705402155 2313 6 -0.174736333260185 2313 7 -0.6017769865337641 2313 8 0.5191489374938811 2313 9 -2.5878897833568217 2313 10-11 -0.08782932181831171 2313 12-13 -0.7118721513778112 2313 14-15 0.10805337449420449 2313 16-17 0.23598703190850046 2313 18-19 0.74900272109209 2313 20-21 -0.20926281272207348 2313 22-23 -1.2859160756253019 2313 24-25 -0.31211119649221786 2313 26-27 -0.09127711149313456 2313 28-29 0.944020138076894 2313 30-31 -1.4937372142101282 2313 32-33 -0.8135699085878088 2313 34-35 0.2748450829721776 2313 36-37 0.06612660473179233 2313 38-39 1.1291695147389547 2313 40-41 1.3774311986761987 2313 42-43 1.514595015787826 2313 44-45 0.4836069987868754 2313 46-47 0.14455639730634573 2313 48-49 1.2938000314284928 2313 50-51 0.020010048665941582 2313 52-53 1.0879316317866703 2313 54-55 -0.09133328112881145 2313 56-57 0.3597096151661425 2313 58-59 0.5593282488623821 2313 60-61 -0.6307382886649897 2313 62-63 0.1536738329200773 2313 64-65 0.5383073775557641 2313 66-67 -0.48092594037457204 2313 68-69 0.9472099441873674 2313 70-71 1.53198699595168 2313 72-73 -0.2592375225717909 2313 74-75 0.5116820623127509 2313 76-77 0.8739339164858784 2313 78-79 1.2429505721753564 2313 80-81 0.8969359621355046 2313 82-83 1.3766924798306874 2313 84-85 1.515508778182312 2313 86-87 1.6806337378291438 2313 88-89 2.046164137357863 2313 90-91 3.0291858496024915 2313 92-93 2.663161024663953 2313 94-95 2.588967160196926 2313 96-97 1.4543681673468196 2313 98-99 1.4601276971200203 2313 100-101 2.061173686333916 2314 1 -14.148856198398676 2314 2 -7.0188392384725375 2314 3 -3.473329325255982 2314 4 -0.9323707030656756 2314 5 -1.1001430341509817 2314 6 -4.752148957151682 2314 7 -0.9431437053652658 2314 8 -2.9263909686094003 2314 9 -1.3638417917031944 2314 10-11 0.6260465258603531 2314 12-13 -2.86033328857134 2314 14-15 -2.6061615446502913 2314 16-17 -2.4927297130054242 2314 18-19 -1.07488877603884 2314 20-21 -1.8644271319709063 2314 22-23 -1.8537303687917088 2314 24-25 -3.2745264286257623 2314 26-27 -6.965820669135283 2314 28-29 -3.3157086047504407 2314 30-31 -2.2570914134798272 2314 32-33 -5.053267352510602 2314 34-35 -4.278493466845248 2314 36-37 -1.6821258730981512 2314 38-39 -2.1344715911556733 2314 40-41 -3.9025635848397116 2314 42-43 -1.965921416137057 2314 44-45 -3.4775823595855755 2314 46-47 -3.1022615473989212 2314 48-49 -2.4735186957493944 2314 50-51 -2.6476216675573205 2314 52-53 -1.802965603476764 2314 54-55 -1.6878643192091474 2314 56-57 -2.4520796388766364 2314 58-59 -2.8676670563019364 2314 60-61 -1.0467007299795448 2314 62-63 -3.162027784189995 2314 64-65 -1.640983180608032 2314 66-67 -3.245766837609832 2314 68-69 -2.060067051117798 2314 70-71 -3.8109185856862986 2314 72-73 -3.114949572650037 2314 74-75 -3.169173441077966 2314 76-77 -2.008460449711304 2314 78-79 -1.328332682910716 2314 80-81 -1.3010556914899531 2314 82-83 -1.4000941659700423 2314 84-85 -1.2704588796163314 2314 86-87 -1.2385107587801407 2314 88-89 -0.22024170510432128 2314 90-91 0.546582824041721 2314 92-93 1.5732288389571174 2314 94-95 -0.676812704174484 2314 96-97 -0.2422150355744126 2314 98-99 0.5631010930511611 2314 100-101 1.230631171988584 2315 1 -14.148856198398676 2315 2 -6.97141051478482 2315 3 -4.682069000182707 2315 4 -2.923257993233541 2315 5 -3.554979165246106 2315 6 -3.5846562846230405 2315 7 -3.684257481011418 2315 8 -3.8410192659715108 2315 9 -2.5871298279408705 2315 10-11 -4.6971615743261665 2315 12-13 -4.343040456170591 2315 14-15 -3.6414399885618245 2315 16-17 -5.5549998968583445 2315 18-19 -1.698763009985015 2315 20-21 -3.8375686187814537 2315 22-23 -5.198521202791174 2315 24-25 -6.6032501163422594 2315 26-27 -5.063209424797421 2315 28-29 -3.193379801126671 2315 30-31 -4.106438602395357 2315 32-33 -5.822385391412816 2315 34-35 -4.611693573426649 2315 36-37 -6.089106954899378 2315 38-39 -3.3258917883312655 2315 40-41 -5.674879065788282 2315 42-43 -4.785106068415239 2315 44-45 -3.510516012663114 2315 46-47 -2.644201328907048 2315 48-49 -2.059503241446169 2315 50-51 -1.331368003821641 2315 52-53 -1.0028323767248324 2315 54-55 -2.2301771356226823 2315 56-57 -5.4838408965371706 2315 58-59 -4.4976430754865895 2315 60-61 -5.4246383798596405 2315 62-63 -3.090271855599532 2315 64-65 -4.165816647168118 2315 66-67 -2.228367423807544 2315 68-69 -2.443280480374394 2315 70-71 -2.8527784311432676 2315 72-73 -2.2834547684933604 2315 74-75 -2.636053270547727 2315 76-77 -2.259539586401953 2315 78-79 -1.6890840817916093 2315 80-81 -1.2281273674824966 2315 82-83 -1.0011066892847253 2315 84-85 -0.09118896221692196 2315 86-87 -1.668033807008225 2315 88-89 -2.184456999535442 2315 90-91 -3.2348986574397607 2315 92-93 -2.3783832047412545 2315 94-95 -2.4692454246647593 2315 96-97 -3.5786392295525626 2315 98-99 -3.2236295224564326 2315 100-101 -3.489566003604274 2316 1 -14.148856198398676 2316 2 -6.989209608842907 2316 3 -4.25110710303376 2316 4 -1.9916299623249358 2316 5 -1.0927356267435755 2316 6 -0.7965934015961267 2316 7 -3.2394400016615634 2316 8 -2.18565022786866 2316 9 -0.27495290281430584 2316 10-11 -2.5665460667322364 2316 12-13 -2.5269999552380042 2316 14-15 -0.5283837668725084 2316 16-17 -2.7853223055980223 2316 18-19 -1.5489628501129147 2316 20-21 -2.564427131970902 2316 22-23 -2.305582220643565 2316 24-25 -2.800452354551691 2316 26-27 -3.6880428913575045 2316 28-29 -3.6749678640097017 2316 30-31 -2.542276598665012 2316 32-33 -2.0940080932513467 2316 34-35 -3.0044193927711724 2316 36-37 -1.7821258730981526 2316 38-39 -2.549286405970488 2316 40-41 -2.2507117329878596 2316 42-43 -6.136291786507428 2316 44-45 -2.6738786558818717 2316 46-47 -4.117076362213737 2316 48-49 -3.529074251304948 2316 50-51 -2.777251297186954 2316 52-53 -4.195558196069356 2316 54-55 -3.924901356246181 2316 56-57 -3.4965240833210807 2316 58-59 -3.071370760005639 2316 60-61 -1.45040443368325 2316 62-63 -0.2768425990048087 2316 64-65 -0.27431651394136836 2316 66-67 -1.2346557264987226 2316 68-69 -1.4748818659326126 2316 70-71 -1.3109185856862986 2316 72-73 -2.914949572650034 2316 74-75 -3.8876919595964843 2316 76-77 -2.501053042303898 2316 78-79 -2.9283326829107175 2316 80-81 -3.3714260618603227 2316 82-83 -2.9445386104144866 2316 84-85 -2.3815699907274457 2316 86-87 -2.2977700180393974 2316 88-89 -2.223945408808021 2316 90-91 -2.3311949537360555 2316 92-93 -1.1786230128947324 2316 94-95 -0.9805164078781878 2316 96-97 -1.7125854059447807 2316 98-99 -1.5368989069488386 2316 100-101 -0.9397391983817869 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2090.0 3 361.0 4 276.0 5 305.0 6 354.0 7 437.0 8 540.0 9 645.0 10 832.0 11 978.0 12 1140.0 13 1337.0 14 1413.0 15 1683.0 16 1801.0 17 1961.0 18 1879.0 19 2035.0 20 2166.0 21 2462.0 22 2674.0 23 2896.0 24 3395.0 25 3858.0 26 4330.0 27 4667.0 28 5478.0 29 5994.0 30 6781.0 31 7803.0 32 8916.0 33 10348.0 34 12027.0 35 10726.0 36 8095.0 37 2870.0 38 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.172436017028204 18.558029884160717 16.46342497583148 11.806109122979597 2 13.524818739542665 31.062863516851248 28.666241733726395 26.746076009879694 3 34.09817251520247 24.62362420600448 32.391829317861216 8.886373960931834 4 10.912861415752742 17.84247258225324 44.94596211365902 26.298703888334995 5 8.042239489938234 44.250249053596335 35.17354054592548 12.53397091053995 6 22.257563075451394 38.76838455099845 14.558571485511578 24.415480888038584 7 18.408737593175747 38.289153744967514 24.48200263084466 18.82010603101208 8 20.690204956912016 20.086734002439393 21.809456238390958 37.41360480225763 9 14.684054874889396 30.21626318264793 31.685386093153394 23.41429584930928 10-11 25.256643234023564 23.05764111392728 25.55513047359444 26.13058517845472 12-13 35.59215980104579 24.402180844280068 23.58954533860477 16.41611401606938 14-15 23.665728657637334 29.072566823178654 35.37662524014891 11.885079279035098 16-17 15.256311128470424 31.898400197683596 33.29733047436091 19.547958199485066 18-19 14.431539172858008 40.0094863046459 28.353289117056217 17.205685405439876 20-21 21.095942842561875 28.220687675427403 24.175491196733862 26.50787828527686 22-23 15.882200466572288 29.099754750463582 30.49029968297011 24.527745099994018 24-25 26.844845124359257 28.916369148533022 25.519277180525364 18.719508546582365 26-27 22.279040454404033 22.096752319486882 39.28010147505804 16.34410575105105 28-29 14.850403060145908 39.68696526223839 28.898275676387176 16.564356001228525 30-31 18.20307108006431 28.220584773857922 27.75262206725418 25.82372207882359 32-33 19.95349078391563 29.771083712600166 31.79858238632964 18.476843117154562 34-35 25.644277476011577 26.605010648225697 28.62498305057708 19.125728825185647 36-37 20.296593442086923 24.737121233267274 38.25967676977123 16.70660855487457 38-39 18.95298438129264 30.736994046788336 33.62826918246016 16.681752389458868 40-41 17.91208440662557 33.63744387645243 30.335026676130244 18.11544504079175 42-43 15.873224723491465 33.595288788944444 23.840338747876846 26.69114773968725 44-45 20.656335242227506 24.994018089297963 28.043197371149642 26.306449297324892 46-47 26.46808918035926 31.70577980956028 29.01991758515735 12.80621342492311 48-49 21.811073879048987 28.57268230413276 30.990505824158287 18.625737992659968 50-51 11.055210220154644 31.368645318821265 39.871609706274285 17.704534754749805 52-53 18.946217420974286 35.925820985461904 24.9993016286021 20.12865996496171 54-55 14.450908568555626 26.823720941368002 31.3394303590382 27.385940131038172 56-57 22.724244539742696 24.655430338087164 28.75196968185898 23.86835544031116 58-59 29.99425809847361 24.943378471059223 27.065489576853757 17.99687385361341 60-61 20.34878388240408 28.392826521293525 36.59479097293396 14.66359862336844 62-63 15.760843556080209 29.55517092858168 34.495589197122214 20.188396318215897 64-65 15.029927464280446 37.684678972616666 28.81570184988017 18.469691713222716 66-67 20.082632433230042 27.570598486933147 25.098006372906774 27.248762706930034 68-69 15.629784994258006 28.57797945642465 30.628349495980604 25.163886053336736 70-71 25.594499854440755 28.200209764832092 25.51314619332661 20.69214418740055 72-73 20.89913006712962 22.279615011826458 37.62409805790777 19.197156863136147 74-75 15.232406420953087 37.460746876708285 28.22913050591141 19.077716196427215 76-77 18.58834701971957 26.61024523769622 27.877931799500427 26.923475943083787 78-79 19.768671276217987 27.86557558879504 31.47435155741924 20.891401577567738 80-81 24.60199024826232 26.11263177215089 28.3518605708996 20.933517408687187 82-83 19.631272791107776 24.329931457114814 35.76677864398394 20.27201710779346 84-85 18.480437123599092 29.14768874885335 31.91680293542855 20.45507119211901 86-87 18.534207671197905 31.150280809241178 29.366105048184316 20.9494064713766 88-89 17.53091344236139 30.423214998005584 24.46150777822098 27.584363781412048 90-91 20.79186991221388 23.669946514679545 28.307734033176057 27.230449539930525 92-93 24.82021705574767 29.254663587522284 29.18247121279201 16.742648143938034 94-95 21.297556109725686 27.13137157107232 29.89925187032419 21.671820448877803 96-97 13.175036520822855 28.597201267651727 36.11769683326548 22.110065378259932 98-99 19.9849896005078 31.81845400869485 25.346017653187914 22.850538737609437 100-101 17.416333651377904 25.846244745278234 29.012451644583194 27.724969958760664 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6607.0 1 4119.5 2 1297.5 3 768.5 4 551.5 5 616.0 6 1015.5 7 1596.0 8 1629.0 9 1114.5 10 651.5 11 386.0 12 267.0 13 213.0 14 175.5 15 147.5 16 123.0 17 126.0 18 124.5 19 108.5 20 119.0 21 134.5 22 143.0 23 153.0 24 177.0 25 206.5 26 254.0 27 285.0 28 318.5 29 362.0 30 391.0 31 416.5 32 453.5 33 476.0 34 486.0 35 467.5 36 437.0 37 408.0 38 358.5 39 334.5 40 310.0 41 298.0 42 340.5 43 461.5 44 825.5 45 2433.5 46 7215.5 47 19245.0 48 26513.5 49 20422.0 50 11268.0 51 5056.0 52 2451.5 53 1331.0 54 754.5 55 443.0 56 297.5 57 229.5 58 185.0 59 147.0 60 118.5 61 102.5 62 99.5 63 87.5 64 77.5 65 63.5 66 42.0 67 28.0 68 23.0 69 15.0 70 7.0 71 4.0 72 2.5 73 4.5 74 4.0 75 3.5 76 2.5 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.06811216976701 2 0.09631380789773225 3 0.12576513758546856 4 0.20377136216379715 5 0.1241731738185639 6 0.14805263032213387 7 0.15601244915665718 8 0.1512365578559432 9 0.14486870278832453 10-11 0.1313370107696349 12-13 0.1392968296041582 14-15 0.14964459408903852 16-17 0.1416847752545152 18-19 0.1488486122055862 20-21 0.17750396000987018 22-23 0.19780149803790467 24-25 0.22964077337599798 26-27 0.22287492736665312 28-29 0.22088497265802232 30-31 0.23919255597742595 32-33 0.22247693642492697 34-35 0.2053633259307018 36-37 0.19143364297028598 38-39 0.18824971543647667 40-41 0.18944368826165517 42-43 0.18148386942713185 44-45 0.20138341651344016 46-47 0.2304367552594503 48-49 0.2316307280846288 50-51 0.24675438387022308 52-53 0.2702358494320669 54-55 0.2578981302385558 56-57 0.23362068279325962 58-59 0.18864770637820283 60-61 0.20098542557171398 62-63 0.2045673440472495 64-65 0.1946175705040953 66-67 0.2041693531055233 68-69 0.1902396701451075 70-71 0.20098542557171398 72-73 0.22008899077456995 74-75 0.25670415741337727 76-77 0.2578981302385558 78-79 0.2479483566954016 80-81 0.2547142027047465 82-83 0.24516242010331843 84-85 0.21173118099832047 86-87 0.22207894548320078 88-89 0.22367090925010544 90-91 0.21451711759040365 92-93 0.2161090813573083 94-95 0.25352022987956796 96-97 0.28655347804283976 98-99 0.3060550341874219 100-101 0.30764699795432654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 125631.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.38888490897947 #Duplication Level Percentage of deduplicated Percentage of total 1 86.70365535248041 21.14605471579467 2 5.930156657963447 2.892598164465777 3 2.196475195822454 1.6070874226902596 4 1.2859007832898173 1.2544674483208762 5 0.7832898172323759 0.955178260142799 6 0.4765013054830287 0.6972801299042434 7 0.35574412532637073 0.6073341770741297 8 0.27415143603133163 0.5348998256799675 9 0.18603133159268928 0.4083387062110466 >10 1.3152741514360313 6.246069839450454 >50 0.1664490861618799 2.8822503999808964 >100 0.18603133159268928 10.529248354307457 >500 0.05548302872062663 10.039719495984272 >1k 0.08159268929503916 36.04285566460507 >5k 0.0032637075718015664 4.156617395388081 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5222 4.156617395388081 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4467 3.5556510733815694 No Hit NCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3503 2.788324537733521 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2998 2.3863536865900934 No Hit NAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2697 2.146763139670941 No Hit NTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2448 1.9485636506913102 No Hit NCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2300 1.830758331940365 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2122 1.68907355668585 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2039 1.6230070603593063 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1861 1.481322285104791 No Hit NTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1724 1.3722727670718213 No Hit NTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1529 1.2170562997986165 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1478 1.1764612237425476 No Hit NCGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1464 1.165317477374215 No Hit NATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1463 1.1645214954907626 No Hit NGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1355 1.0785554520779106 No Hit NGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1316 1.0475121586232696 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1310 1.0427362673225558 No Hit NTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1274 1.0140809195182718 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1215 0.9671179883945842 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1211 0.9639340608607747 No Hit NCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1200 0.955178260142799 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1162 0.9249309485716105 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1098 0.8739881080306612 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1031 0.820657321839355 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1016 0.80871759358757 No Hit NCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 976 0.7768783182494766 No Hit NTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 920 0.732303332776146 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 890 0.7084238762725761 No Hit NATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 833 0.663052908915793 No Hit NAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 810 0.6447453255963894 No Hit NTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 785 0.6248457785100812 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 769 0.6121100683748438 No Hit NGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 756 0.6017623038899635 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 754 0.6001703401230588 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 708 0.5635551734842514 No Hit NACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 698 0.5555953546497282 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 680 0.5412676807475862 No Hit NTACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 679 0.5404716988641338 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 639 0.5086324235260405 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 597 0.4752011844210426 No Hit NCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 576 0.4584855648685436 No Hit NTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 543 0.4322181627146166 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 498 0.39639897795926166 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 488 0.3884391591247383 No Hit NGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 480 0.38207130405711964 No Hit NGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 476 0.37888737652331034 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 461 0.3669476482715253 No Hit NCTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 434 0.3454561374183123 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 412 0.327944535982361 No Hit NAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 411 0.3271485540989087 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 383 0.3048610613622434 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 382 0.3040650794787911 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.3032690975953387 No Hit NATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 360 0.28655347804283976 No Hit NCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 330 0.2626740215392698 No Hit NTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 324 0.2578981302385558 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 293 0.23322269185153346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 291 0.2316307280846288 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 284 0.22605885490046249 No Hit NTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 274 0.21809903606593914 No Hit NACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 266 0.21173118099832047 No Hit NACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 262 0.20854725346451114 No Hit NGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 253 0.20138341651344016 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 247 0.19660752521272618 No Hit NAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 230 0.1830758331940365 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 215 0.1711361049422515 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 212 0.16874815929189452 No Hit NTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 202 0.16078834045737117 No Hit NGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 196 0.15601244915665718 No Hit NTCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 193 0.1536245035063002 No Hit NTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 190 0.1512365578559432 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 177 0.14088879337106286 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 175 0.1392968296041582 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 175 0.1392968296041582 No Hit NCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 174 0.13850084772070587 No Hit NTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 169 0.1345209383034442 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 166 0.13213299265308723 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 161 0.12815308323582555 No Hit NAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 161 0.12815308323582555 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 156 0.1241731738185639 No Hit NAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 142 0.11302942745023124 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 141 0.11223344556677892 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 137 0.10904951803296957 No Hit NAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.10904951803296957 No Hit NGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 131 0.10427362673225557 No Hit NCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 127 0.10108969919844625 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.1002937173149939 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.979909417261663E-4 0.0 20-21 0.0 0.0 0.0 0.0019899547086308315 0.0 22-23 0.0 0.0 0.0 0.006765846009344827 0.0 24-25 0.0 0.0 0.0 0.014725664843868154 0.0 26-27 0.0 0.0 0.0 0.028257356862557807 0.0 28-29 0.0 0.0 0.0 0.06925042386035293 0.0 30-31 0.0 0.0 0.0 0.1504405759724909 0.0 32-33 0.0 0.0 0.0 0.33192444539962274 0.0 34-35 0.0 0.0 0.0 0.5579833003000851 0.0 36-37 0.0 0.0 0.0 0.7995638019278681 0.0 38-39 0.0 0.0 0.0 1.1187525371922535 0.0 40-41 0.0 0.0 0.0 1.4638106836688398 0.0 42-43 0.0 0.0 0.0 1.7642938446720953 0.0 44-45 0.0 0.0 0.0 2.114127882449395 0.0 46-47 0.0 0.0 0.0 2.5352022987956793 0.0 48-49 0.0 0.0 0.0 2.852003088409708 0.0 50-51 0.0 0.0 0.0 3.1922853435855796 0.0 52-53 0.0 0.0 0.0 3.5043102418988945 0.0 54-55 0.0 0.0 0.0 3.8871775278394667 0.0 56-57 0.0 0.0 0.0 4.491327777379787 0.0 58-59 0.0 0.0 0.0 4.88653278251387 0.0 60-61 0.0 0.0 0.0 5.382429495904673 0.0 62-63 0.0 0.0 0.0 5.874346299878215 0.0 64-65 0.0 0.0 0.0 6.31293231766045 0.0 66-67 0.0 0.0 0.0 6.903948866123807 0.0 68-69 0.0 0.0 0.0 7.60640287827049 0.0 70-71 0.0 0.0 0.0 8.239606466556822 0.0 72-73 0.0 0.0 0.0 8.746248935374231 0.0 74-75 0.0 0.0 0.0 9.497655833353232 0.0 76-77 0.0 0.0 0.0 10.0325556590332 0.0 78-79 0.0 0.0 0.0 11.042258678192486 0.0 80-81 0.0 0.0 0.0 12.015744521654687 0.0 82-83 0.0 0.0 0.0 12.67680747586185 0.0 84-85 0.0 0.0 0.0 13.550397592950784 0.0 86-87 0.0 0.0 0.0 14.348369431111749 0.0 88-89 0.0 0.0 0.0 15.27927024380925 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGA 20 1.7870987E-5 94.465706 4 GAGTATA 40 0.001647269 75.42021 1 ACCCATG 35 2.856827E-4 53.980404 3 GCTTTGC 175 9.458745E-11 51.71671 1 CGCAGAA 265 0.0 44.52379 9 CTTTGCG 245 0.0 38.557434 2 TTTGCGT 245 0.0 38.557434 3 GCTTGAT 155 2.6422297E-4 32.438797 1 TTGCGTT 295 0.0 32.022274 4 CGCCGTA 45 2.4796576E-5 31.526281 94-95 CAGAAAA 235 0.0 30.124607 10-11 TGCTTTG 165 2.7848728E-9 28.60316 7 GTTGATC 85 3.787136E-9 27.817307 94-95 TGCATAC 35 0.008579527 27.011745 78-79 AGTACTT 520 0.0 26.32041 12-13 GCAGAAA 240 0.0 23.597609 10-11 TACTTTT 595 0.0 23.39931 14-15 TGGTCGC 65 3.0139112E-4 21.834602 90-91 GAGAAAA 105 9.097912E-7 20.226522 12-13 GAGTACG 85 8.172195E-5 19.47989 92-93 >>END_MODULE