##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 125631 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.252955082742318 31.0 31.0 33.0 30.0 34.0 2 31.368531652219595 31.0 31.0 34.0 30.0 34.0 3 31.40451003335164 31.0 31.0 34.0 28.0 34.0 4 35.08087175935876 35.0 35.0 37.0 32.0 37.0 5 34.89600496692695 35.0 35.0 37.0 32.0 37.0 6 35.01925480176071 36.0 35.0 37.0 32.0 37.0 7 35.00369335593922 36.0 35.0 37.0 32.0 37.0 8 35.067109232593864 37.0 35.0 37.0 32.0 37.0 9 36.437121411116685 38.0 35.0 39.0 32.0 39.0 10-11 36.28565799842396 38.0 35.0 39.0 31.5 39.0 12-13 36.374505496254905 38.0 35.0 39.0 32.0 39.0 14-15 36.960479499486596 38.0 36.0 40.0 31.0 41.0 16-17 36.98820752839666 38.0 36.0 40.0 31.5 41.0 18-19 36.92259474174368 38.5 35.5 40.0 31.5 41.0 20-21 36.70744481855593 38.0 35.0 40.0 31.0 41.0 22-23 36.55329098709713 38.0 35.0 40.0 31.0 41.0 24-25 36.16553637239217 38.0 34.5 40.0 30.0 41.0 26-27 35.692766912625075 37.5 34.5 40.0 28.5 41.0 28-29 35.676899013778446 37.5 34.0 40.0 30.0 41.0 30-31 35.54553414364289 37.0 34.0 40.0 29.0 41.0 32-33 35.25145863680142 37.0 34.0 40.0 28.0 41.0 34-35 35.072633346865025 37.0 34.0 40.0 27.0 41.0 36-37 34.90510701976423 37.0 33.5 40.0 27.0 41.0 38-39 34.75318989739794 37.0 33.0 40.0 25.5 41.0 40-41 35.061179167562145 37.5 33.0 40.0 27.0 41.0 42-43 34.78638632184732 37.0 33.0 40.0 26.0 41.0 44-45 34.57430092891086 37.0 33.0 40.0 24.5 41.0 46-47 34.291472646082575 37.0 33.0 40.0 24.0 41.0 48-49 33.88331701570472 37.0 33.0 40.0 23.0 41.0 50-51 33.552658181499794 37.0 33.0 40.0 23.0 41.0 52-53 33.62720586479452 37.0 33.0 40.0 23.0 41.0 54-55 33.53757034489895 37.0 33.0 40.0 22.5 41.0 56-57 32.50838566914217 35.5 31.5 39.5 17.5 40.5 58-59 29.32099959404924 34.5 24.5 38.5 7.0 40.0 60-61 28.8437009973653 33.5 24.0 38.0 7.5 40.0 62-63 30.6899610764859 34.5 29.0 38.0 8.0 40.0 64-65 30.840513089922077 35.0 30.0 38.0 2.0 39.5 66-67 25.725286752473515 30.0 18.0 36.0 2.0 38.5 68-69 18.713565919239677 19.0 7.0 31.0 2.0 36.0 70-71 16.532201447095062 13.0 7.0 27.0 2.0 34.0 72-73 18.237891125597983 17.0 7.0 29.5 2.0 34.0 74-75 19.740677062190066 22.0 7.5 31.5 2.0 35.0 76-77 21.412270856715303 26.5 9.0 32.0 2.0 34.0 78-79 23.373080688683526 29.5 10.5 34.0 2.0 35.0 80-81 24.610120113666213 30.5 16.5 34.0 2.0 35.0 82-83 25.551866179525753 32.0 22.5 34.0 2.0 35.0 84-85 25.536897740207433 32.0 23.0 34.0 2.0 35.0 86-87 25.68389569453399 33.0 23.5 34.5 2.0 35.0 88-89 25.122008103095574 32.0 19.0 34.0 2.0 35.0 90-91 23.368404295118243 31.0 6.0 34.0 2.0 35.0 92-93 21.96170133167769 30.5 2.0 34.0 2.0 35.0 94-95 18.60220009392586 25.0 2.0 33.0 2.0 35.0 96-97 16.14090471300873 10.0 2.0 32.0 2.0 34.0 98-99 16.42684926491073 8.5 2.0 32.5 2.0 34.5 100-101 15.653907077074926 2.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4055577271105264 1101 2 0.6497016961683748 1101 3 0.6530640286721088 1101 4 0.4980148472121826 1101 5 0.26242529371995005 1101 6 0.4675023018742337 1101 7 0.810350616123408 1101 8 0.8252500580751985 1101 9 0.7366167715472329 1101 10-11 0.49837031408517163 1101 12-13 0.4395284159217496 1101 14-15 0.8473676628189466 1101 16-17 1.263501399539102 1101 18-19 0.6213897275568243 1101 20-21 0.6258389184426036 1101 22-23 0.8967998485623738 1101 24-25 1.2467431694244127 1101 26-27 1.1663954358343105 1101 28-29 1.0072415063230054 1101 30-31 1.0683953552761238 1101 32-33 1.272268137971544 1101 34-35 1.059201187635928 1101 36-37 0.8620278992508617 1101 38-39 1.117623927626667 1101 40-41 1.0361303024759465 1101 42-43 1.7437739647804094 1101 44-45 0.6286292270566065 1101 46-47 0.9777329088221549 1101 48-49 1.639919064328467 1101 50-51 1.8334309814779957 1101 52-53 1.1927188404139315 1101 54-55 1.7772141232419116 1101 56-57 -0.8812067287276264 1101 58-59 -9.241107969249178 1101 60-61 -11.31460219399888 1101 62-63 -2.615497462822418 1101 64-65 0.40106500566155745 1101 66-67 -0.4829874707044688 1101 68-69 -3.1002333291986552 1101 70-71 -2.087771967462329 1101 72-73 -0.7035234485562079 1101 74-75 -3.7537247489984065 1101 76-77 -2.554769614602794 1101 78-79 3.2283120714083324 1101 80-81 4.220207511667219 1101 82-83 2.588600497773193 1101 84-85 1.9939745221403449 1101 86-87 1.278432268766931 1101 88-89 1.0247336806987484 1101 90-91 2.728575212568945 1101 92-93 2.04075703640142 1101 94-95 -8.061460734334485 1101 96-97 -9.001997342860546 1101 98-99 -8.681583059910334 1101 100-101 -9.72348058565672 1102 1 0.4828910604438583 1102 2 0.38782290828958565 1102 3 0.5608822104902877 1102 4 0.5542572714546026 1102 5 0.4578798391744954 1102 6 0.5447750291469617 1102 7 0.39174455551734866 1102 8 0.7001591489842909 1102 9 0.7627985897290515 1102 10-11 0.7837642534791129 1102 12-13 0.5101950825884174 1102 14-15 0.5062464506977307 1102 16-17 0.49289533893303883 1102 18-19 0.7700563942234879 1102 20-21 1.1198692214729036 1102 22-23 1.126678636441163 1102 24-25 0.912197714878964 1102 26-27 1.2019408903797668 1102 28-29 1.193211203292698 1102 30-31 1.3048802037609661 1102 32-33 1.037359047062445 1102 34-35 0.8567466421813847 1102 36-37 1.25690668712965 1102 38-39 1.1102299882327316 1102 40-41 1.250706060051705 1102 42-43 1.5932588132652512 1102 44-45 1.354386802814183 1102 46-47 1.1502783633676117 1102 48-49 0.9723736097830198 1102 50-51 1.8083703754173897 1102 52-53 1.1606885373836278 1102 54-55 1.2447595777873701 1102 56-57 -1.3694794560003558 1102 58-59 -10.34922918137039 1102 60-61 -7.334056739453427 1102 62-63 -1.519588371913322 1102 64-65 -0.15681378221723108 1102 66-67 -0.9635632282802256 1102 68-69 -3.7705060564713833 1102 70-71 -2.5149537856441473 1102 72-73 -1.2597355697683277 1102 74-75 -2.550027779301434 1102 76-77 -3.7216787055118843 1102 78-79 2.67158479868106 1102 80-81 3.1128438753035823 1102 82-83 2.469509588682282 1102 84-85 1.601398764564589 1102 86-87 1.8414322687669298 1102 88-89 2.1591882261532938 1102 90-91 3.9131812731750095 1102 92-93 1.8757267333711134 1102 94-95 -7.439763764637515 1102 96-97 -9.314936736799941 1102 98-99 -7.678946696273972 1102 100-101 -8.68008664626278 1103 1 0.2706370921898902 1103 2 0.12769592416260167 1103 3 0.11662824223632029 1103 4 -0.03806018886285756 1103 5 0.2801020613967182 1103 6 0.07175915613108685 1103 7 0.07117312694591504 1103 8 0.3764766093017542 1103 9 0.1897192246496857 1103 10-11 0.5528436185584766 1103 12-13 0.3720680984614333 1103 14-15 0.5777067681580519 1103 16-17 -0.15424751820982152 1103 18-19 0.04996115612824781 1103 20-21 0.4229168405205215 1103 22-23 0.8592818110443403 1103 24-25 0.9191818418630859 1103 26-27 1.1189567633956372 1103 28-29 0.3292112032927008 1103 30-31 0.7224357593165252 1103 32-33 0.6932638089672096 1103 34-35 0.7022387056734445 1103 36-37 0.23125589347885267 1103 38-39 0.6464839564866978 1103 40-41 -0.2717701304244855 1103 42-43 -0.5644872184807781 1103 44-45 0.2877201361475201 1103 46-47 0.1425958236850704 1103 48-49 0.22704027644968505 1103 50-51 -0.020645497598486884 1103 52-53 0.2607202834153739 1103 54-55 -0.4448277237999392 1103 56-57 -0.011257233778131592 1103 58-59 -8.774657752798959 1103 60-61 -6.284659914056604 1103 62-63 -1.2487947211196726 1103 64-65 -0.5653852107886621 1103 66-67 -2.675087037804037 1103 68-69 -2.3691727231380497 1103 70-71 -2.4555569602473213 1103 72-73 -1.92989429992706 1103 74-75 -2.946726191999847 1103 76-77 0.17971811988494224 1103 78-79 3.1778070209032805 1103 80-81 2.7088438753035824 1103 82-83 1.683477842650536 1103 84-85 1.5479067010725238 1103 86-87 1.05025766559233 1103 88-89 1.0245533055183742 1103 90-91 1.8606098446035766 1103 92-93 1.7178854635298428 1103 94-95 -5.9052558281295795 1103 96-97 -7.977285943149147 1103 98-99 -7.188978442305718 1103 100-101 -7.625356487532622 1104 1 0.41772317723217967 1104 2 0.18846524405600817 1104 3 0.31480921778955917 1104 4 0.03904559262248597 1104 5 -0.29978439440214544 1104 6 0.18565094155572126 1104 7 0.4595985701158867 1104 8 0.047546010298162855 1104 9 0.5620978598020443 1104 10-11 0.16221680822363282 1104 12-13 0.32882281981469674 1104 14-15 0.18542893244956105 1104 16-17 0.4236982586410747 1104 18-19 0.574552744588452 1104 20-21 0.4535042579692501 1104 22-23 0.5295691473900632 1104 24-25 0.4199349411563347 1104 26-27 0.5123642480440012 1104 28-29 0.4459995244605821 1104 30-31 0.7489969920821409 1104 32-33 0.6412130616609915 1104 34-35 0.2573597808675103 1104 36-37 0.0541037674216156 1104 38-39 0.9410037108604712 1104 40-41 0.612136716986015 1104 42-43 -0.0820112597274445 1104 44-45 0.41424081741271834 1104 46-47 0.7698696042435174 1104 48-49 0.4764320039436001 1104 50-51 1.0640492075341754 1104 52-53 0.7281921870186636 1104 54-55 0.6925260011450263 1104 56-57 2.1935278432697203 1104 58-59 -7.526221882100316 1104 60-61 -5.697647980329339 1104 62-63 -0.34119421132938044 1104 64-65 1.297492787125833 1104 66-67 0.21290392500444355 1104 68-69 -1.4141848885881707 1104 70-71 -1.6258442965930513 1104 72-73 0.04843961271342323 1104 74-75 -1.4026409179875685 1104 76-77 2.0550512214954146 1104 78-79 4.444635893571572 1104 80-81 4.701544605230591 1104 82-83 2.5900935302881223 1104 84-85 2.3249024141996237 1104 86-87 2.8578118308107285 1104 88-89 2.3630714378321294 1104 90-91 3.6176484264596773 1104 92-93 3.5169238136630874 1104 94-95 -3.482274713542626 1104 96-97 -5.588454984975124 1104 98-99 -3.0268883021133863 1104 100-101 -2.484816573270079 1105 1 0.10252742408022186 1105 2 0.09667139313807027 1105 3 0.19851857412665197 1105 4 0.17225727145460468 1105 5 0.42909196038662145 1105 6 0.41447199884392916 1105 7 0.16641122218400994 1105 8 0.27221975504490104 1105 9 0.4941925291229907 1105 10-11 0.2635218292366872 1105 12-13 0.5342253856187256 1105 14-15 0.5443373597886421 1105 16-17 0.5665317025693994 1105 18-19 0.6251776063446997 1105 20-21 0.7925055851092679 1105 22-23 0.3513453031078342 1105 24-25 0.5687128663941152 1105 26-27 1.0679105873494592 1105 28-29 0.9504233245048255 1105 30-31 0.9396074764882414 1105 32-33 0.5374196531230595 1105 34-35 0.8129890664238033 1105 36-37 0.7559672931902597 1105 38-39 0.3714118064145495 1105 40-41 0.8884030297486731 1105 42-43 1.2172588132652535 1105 44-45 1.295295893723278 1105 46-47 1.5345510906403348 1105 48-49 1.3482523976617955 1105 50-51 1.4849461329931444 1105 52-53 1.5222642949593848 1105 54-55 0.8756989717267558 1105 56-57 2.054399331878429 1105 58-59 -7.84716857530978 1105 60-61 -6.625208254604942 1105 62-63 -0.36928534161029347 1105 64-65 1.2684892480857997 1105 66-67 -1.0435935313105311 1105 68-69 -2.0585666625319874 1105 70-71 -2.299893179583542 1105 72-73 -2.0444325394652996 1105 74-75 -2.2537247489984047 1105 76-77 1.827806142972964 1105 78-79 4.993463586559848 1105 80-81 4.822480238939949 1105 82-83 3.391630800803494 1105 84-85 2.4712472494130715 1105 86-87 1.899644389979052 1105 88-89 2.195188226153295 1105 90-91 3.8156964246901595 1105 92-93 3.9422721879165685 1105 94-95 -4.182672855546606 1105 96-97 -5.251997342860546 1105 98-99 -2.935370938698213 1105 100-101 -3.136359373535507 1106 1 -2.3937433875076408E-4 1106 2 0.37993885031857033 1106 3 0.12836047135985496 1106 4 0.27732973522272175 1106 5 0.3912131725078325 1106 6 0.26361560885710844 1106 7 0.42728078740140063 1106 8 0.49586929391183077 1106 9 0.1779869955261546 1106 10-11 0.2819671520298357 1106 12-13 0.41416609708117136 1106 14-15 0.5573478999730952 1106 16-17 0.5362286722663754 1106 18-19 0.6866071188611684 1106 20-21 0.7132895113279787 1106 22-23 0.9129395060063814 1106 24-25 1.234226700386209 1106 26-27 1.129998861394256 1106 28-29 1.1951532322782086 1106 30-31 1.1089091892682177 1106 32-33 1.0944025253233178 1106 34-35 1.214833598703116 1106 36-37 1.329254513216604 1106 38-39 1.873882162145776 1106 40-41 1.5962422919357664 1106 42-43 1.3150849002217768 1106 44-45 1.505111440495341 1106 46-47 2.124307348874858 1106 48-49 1.7234170880438882 1106 50-51 1.8922254478811524 1106 52-53 1.9277320156444944 1106 54-55 1.6502378386569276 1106 56-57 2.415071268637327 1106 58-59 -7.6959828045587955 1106 60-61 -5.3636799278592235 1106 62-63 -0.0010376472756448152 1106 64-65 1.4718818699566754 1106 66-67 2.315596192009629 1106 68-69 -0.21699881009456945 1106 70-71 0.10310418537034494 1106 72-73 0.7567861693621083 1106 74-75 0.23046497432175173 1106 76-77 3.0898285408649286 1106 78-79 5.7780485667970005 1106 80-81 4.66536561443402 1106 82-83 3.346176255348947 1106 84-85 2.223717605144298 1106 86-87 2.3490844426799775 1106 88-89 2.158956342095326 1106 90-91 3.6080218528851518 1106 92-93 2.549320936269666 1106 94-95 -4.591763764637516 1106 96-97 -6.65861789622023 1106 98-99 -3.2617872759841156 1106 100-101 -1.9507533129294483 1107 1 0.3765722198641477 1107 2 0.35819971988378896 1107 3 0.18633148585260884 1107 4 0.2483442279763466 1107 5 0.050633462362903 1107 6 0.2926011161034836 1107 7 0.42003441058980684 1107 8 0.5973185692741438 1107 9 0.12001598103340427 1107 10-11 0.40877874623273414 1107 12-13 0.3634414594000148 1107 14-15 0.6696667405528061 1107 16-17 0.4528953389330397 1107 18-19 0.16124480002059016 1107 20-21 0.14444893161782346 1107 22-23 -0.01821991428347758 1107 24-25 -0.16070083584568096 1107 26-27 -0.19246490672168903 1107 28-29 0.12631265256806046 1107 30-31 0.014706290717484194 1107 32-33 0.5944025253233249 1107 34-35 0.11338432334080295 1107 36-37 -0.3192962114210758 1107 38-39 -0.27829175089770786 1107 40-41 0.012908958602430687 1107 42-43 -0.30085712876373094 1107 44-45 -0.2593813131278466 1107 46-47 -0.16192453518311822 1107 48-49 -0.2983220423909003 1107 50-51 -0.6838615086405824 1107 52-53 -0.032412911891732676 1107 54-55 -0.933095494676401 1107 56-57 0.08898431211558488 1107 58-59 -8.960475558181983 1107 60-61 -7.8093321017722666 1107 62-63 -2.8995883719133246 1107 64-65 -1.7527558112027393 1107 66-67 -0.8728096050918204 1107 68-69 -2.756853882558339 1107 70-71 -2.8280552349195105 1107 72-73 -0.9388660045509383 1107 74-75 -1.2622886488666545 1107 76-77 1.2238865118794209 1107 78-79 3.3034108856375823 1107 80-81 2.3755105419702467 1107 82-83 0.6903791538996735 1107 84-85 -0.04077514847888963 1107 86-87 0.4795192252886693 1107 88-89 0.5393911247040215 1107 90-91 1.7783117079576165 1107 92-93 0.8790310811972013 1107 94-95 -6.714952170434618 1107 96-97 -7.2854294904231285 1107 98-99 -4.0154104643899124 1107 100-101 -3.957999689741042 1108 1 0.11845310423947808 1108 2 -0.06700920849873526 1108 3 -0.10124917637102371 1108 4 -0.3551149913191196 1108 5 0.13817180997741474 1108 6 0.010468459803895769 1108 7 -0.6571897510519946 1108 8 0.0694438205171366 1108 9 -0.07293863654832222 1108 10-11 0.3373992899754583 1108 12-13 -0.39015528237508335 1108 14-15 0.2365238229605069 1108 16-17 -0.04710466106696032 1108 18-19 -0.18092170796629148 1108 20-21 -0.10124026757819848 1108 22-23 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-0.028618344889238756 2312 58-59 3.3280208186296107 2312 60-61 3.080915482768795 2312 62-63 0.5969394058644539 2312 64-65 0.1548528844494328 2312 66-67 -1.0843132282802266 2312 68-69 -2.9490338342491604 2312 70-71 -3.5031760078663687 2312 72-73 -5.6962633475461075 2312 74-75 -6.513194445968104 2312 76-77 -4.110956483289662 2312 78-79 0.06669590979216977 2312 80-81 1.944954986414693 2312 82-83 0.9156206997933936 2312 84-85 0.99334320900903 2312 86-87 0.9450989354335952 2312 88-89 0.6743548928199594 2312 90-91 2.007931273175007 2312 92-93 2.6161989555933367 2312 94-95 4.522819568695816 2312 96-97 0.44086881875561446 2312 98-99 -1.2658633629406388 2312 100-101 -0.15908664626278046 2313 1 -0.4442075311054374 2313 2 -0.6487404719921059 2313 3 -0.14046990218576738 2313 4 -0.9721089257284916 2313 5 -0.16043001998043138 2313 6 -0.6538165201488155 2313 7 -0.46504417687702215 2313 8 -0.9007422594664121 2313 9 -0.4733704243554513 2313 10-11 -1.0868836338448347 2313 12-13 -0.23887533994679444 2313 14-15 -0.6904577746543765 2313 16-17 -0.6562595906444244 2313 18-19 -0.5851830423962312 2313 20-21 -1.0940181024707627 2313 22-23 -0.7625044621503889 2313 24-25 -0.6092107358252647 2313 26-27 -1.4160309406061558 2313 28-29 -1.1144507685382834 2313 30-31 -1.4774859934221283 2313 32-33 -1.15666912195163 2313 34-35 -1.1288589916214349 2313 36-37 -1.0666426086449974 2313 38-39 -1.4731784624714948 2313 40-41 -0.81720943290604 2313 42-43 -0.6595017501150338 2313 44-45 -0.5737822112703199 2313 46-47 -0.6874399464915442 2313 48-49 -0.7706123057099461 2313 50-51 -0.8307282161319094 2313 52-53 -0.8198185048698932 2313 54-55 -0.23831084474785058 2313 56-57 0.4953937834362705 2313 58-59 3.5256440580662307 2313 60-61 3.1857179084338973 2313 62-63 0.6215383886500554 2313 64-65 -0.3339968808087832 2313 66-67 -1.1366899888436066 2313 68-69 -4.353914507175608 2313 70-71 -1.9429256166300632 2313 72-73 -1.1222707810359331 2313 74-75 -2.0903658074704516 2313 76-77 0.9810818578683964 2313 78-79 3.043612967695143 2313 80-81 1.9694072555852706 2313 82-83 1.0527490253020027 2313 84-85 0.2874551025927552 2313 86-87 0.6604745222880588 2313 88-89 0.9134980853082233 2313 90-91 2.542702399935571 2313 92-93 3.6708253249204112 2313 94-95 4.749785531137132 2313 96-97 2.940330868833863 2313 98-99 2.7964899234443408 2313 100-101 3.1630964523287712 2314 1 -0.5870348654820674 2314 2 -0.573362276895601 2314 3 -0.5040066783985999 2314 4 -0.33700198780465485 2314 5 -0.47545349415883464 2314 6 -0.9589286745567449 2314 7 -0.9928480370752482 2314 8 -0.7431001102749661 2314 9 -0.3637199287894646 2314 10-11 -0.3789024131875536 2314 12-13 -0.3826938063004732 2314 14-15 -1.3379016974504125 2314 16-17 -0.7915491055114003 2314 18-19 -0.587869531702438 2314 20-21 -0.4813159637122837 2314 22-23 -0.5356917339292124 2314 24-25 -0.8078022851210349 2314 26-27 -0.9762813318424577 2314 28-29 -1.3781962041147082 2314 30-31 -1.1571938703131082 2314 32-33 -1.2001224344190362 2314 34-35 -1.2927348393001026 2314 36-37 -1.838056275833317 2314 38-39 -1.573621863619124 2314 40-41 -1.2146272732816286 2314 42-43 -1.3975560015495674 2314 44-45 -1.0567243082969213 2314 46-47 -1.5547586736694328 2314 48-49 -1.6137004642910568 2314 50-51 -2.1735555505085387 2314 52-53 -1.8464225737274838 2314 54-55 -1.9024996814718946 2314 56-57 -1.7853313078522035 2314 58-59 1.3949189667777588 2314 60-61 1.5380914086947186 2314 62-63 -1.810699483024436 2314 64-65 -1.925702671106123 2314 66-67 -3.663711376428374 2314 68-69 -6.558061612026936 2314 70-71 -3.301324156014518 2314 72-73 -3.9997355697683297 2314 74-75 -3.9747685200421756 2314 76-77 -0.6827157425489219 2314 78-79 2.2333625764588376 2314 80-81 1.0199549864146924 2314 82-83 -0.7445644853917912 2314 84-85 -0.7756382724724524 2314 86-87 -0.4428640275293638 2314 88-89 -0.4974043664392944 2314 90-91 0.048440532434266714 2314 92-93 1.1951341407785243 2314 94-95 3.0563843835106326 2314 96-97 1.9809151150519124 2314 98-99 3.386682933355658 2314 100-101 3.8825800204038874 2315 1 -0.6927636158151351 2315 2 -0.9924936384730039 2315 3 -1.0050458470636698 2315 4 -0.7180304983295684 2315 5 -0.4888827507535609 2315 6 -0.73867820826311 2315 7 -1.0506151567128725 2315 8 -0.968732937346644 2315 9 -1.143043136889652 2315 10-11 -0.9813004947223263 2315 12-13 -0.9585531188504248 2315 14-15 -1.2523434773598154 2315 16-17 -1.503939193441063 2315 18-19 -0.8396414475031264 2315 20-21 -1.6745480447141503 2315 22-23 -1.367479636940132 2315 24-25 -1.0567231484303932 2315 26-27 -1.3295986779655564 2315 28-29 -1.7283427535418383 2315 30-31 -1.7629759113469419 2315 32-33 -1.7395042622900618 2315 34-35 -1.6903900484661065 2315 36-37 -1.856388276899132 2315 38-39 -1.8289642563715915 2315 40-41 -2.1100709183655653 2315 42-43 -1.8295037766628042 2315 44-45 -2.2434549238045207 2315 46-47 -2.486786384833831 2315 48-49 -2.5475400592817365 2315 50-51 -1.9677735094747 2315 52-53 -1.710131606501264 2315 54-55 -1.6216576883996865 2315 56-57 -0.7226880890938787 2315 58-59 2.3489290920109056 2315 60-61 2.051813764143695 2315 62-63 -0.8060631920572092 2315 64-65 -0.5864540699870169 2315 66-67 -1.770239487273031 2315 68-69 -4.364376560068504 2315 70-71 -3.6424645770110544 2315 72-73 -2.7393039150920693 2315 74-75 -1.3182004411719426 2315 76-77 1.0089975534809241 2315 78-79 2.282922928177463 2315 80-81 1.2915776882532235 2315 82-83 -0.7719292602385792 2315 84-85 -1.2573062714066374 2315 86-87 -0.5767835585711971 2315 88-89 -0.21128659399058947 2315 90-91 1.303569762383642 2315 92-93 2.307870618263202 2315 94-95 4.785934077089102 2315 96-97 3.2218690185957453 2315 98-99 4.603815893654087 2315 100-101 5.251884576758803 2316 1 -0.0907385691857705 2316 2 0.21182290828958728 2316 3 0.31821554382362294 2316 4 -0.27033532113798486 2316 5 -0.6828609015662437 2316 6 -0.2552249708530425 2316 7 -0.10395914818635532 2316 8 -0.13569270286755852 2316 9 -0.5044606695302036 2316 10-11 -0.1307542650394069 2316 12-13 -0.2752863988930656 2316 14-15 0.021357561808848402 2316 16-17 -0.11747503143732985 2316 18-19 -0.1063880502209571 2316 20-21 -0.6776122600085799 2316 22-23 -0.24309914133661437 2316 24-25 -0.13743191475067107 2316 26-27 -0.9762813318424577 2316 28-29 -0.5670850930035982 2316 30-31 -0.686823499942733 2316 32-33 -0.11864095293754673 2316 34-35 0.22207997551471692 2316 36-37 0.5174992797222444 2316 38-39 0.5078596178623584 2316 40-41 0.0853727267183686 2316 42-43 -0.5827411867347436 2316 44-45 -0.15672430829692985 2316 46-47 -0.1399438588546147 2316 48-49 -0.46925601984661114 2316 50-51 -0.736518513471502 2316 52-53 -0.10197812928304018 2316 54-55 -0.16916634813856035 2316 56-57 0.285039062518166 2316 58-59 3.998622670481467 2316 60-61 3.4640173346206495 2316 62-63 1.4596708873459363 2316 64-65 0.45948251407906326 2316 66-67 -1.2970447097617068 2316 68-69 -1.376580130545456 2316 70-71 -1.149472304162666 2316 72-73 -0.15899482902758777 2316 74-75 5.165972220698567 2316 76-77 5.558024998191819 2316 78-79 4.977807020903281 2316 80-81 3.7162512827109886 2316 82-83 2.259139218311912 2316 84-85 1.7984358016016238 2316 86-87 1.946024861359522 2316 88-89 1.4285215594866294 2316 90-91 3.3262183102120453 2316 92-93 4.96180080744519 2316 94-95 7.563791790918042 2316 96-97 4.7698040039408 2316 98-99 7.045942192614916 2316 100-101 6.427024464848332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 13.0 5 42.0 6 91.0 7 143.0 8 117.0 9 214.0 10 304.0 11 424.0 12 581.0 13 774.0 14 982.0 15 1184.0 16 1534.0 17 1918.0 18 2260.0 19 2218.0 20 2393.0 21 2499.0 22 2560.0 23 2618.0 24 2627.0 25 2630.0 26 3293.0 27 4549.0 28 6419.0 29 8215.0 30 9236.0 31 9868.0 32 10051.0 33 10442.0 34 11026.0 35 10955.0 36 8105.0 37 4448.0 38 892.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.518319771978156 20.54903584337829 17.77917549083613 12.153468893807423 2 13.78338574214863 31.69048282450344 28.34693308920113 26.179198344146798 3 33.383480191990834 24.278243427179596 32.930566500306455 9.40770988052312 4 10.311149318241517 18.836115290016 43.482102347350576 27.370633044391912 5 7.469914062935559 44.739839276185315 35.55357327747557 12.236673383403554 6 21.78761611385725 38.8526717131918 14.35314532241246 25.00656685053848 7 17.312605965088235 38.37269463747005 23.73299583701475 20.581703560426963 8 21.200181483869425 20.92397577031147 21.857662519601053 36.01818022621805 9 13.443338029626444 29.732311292594982 31.497799110092256 25.326551567686316 10-11 25.063081564263594 23.361272297442508 25.988410503776933 25.587235634516958 12-13 34.764110768838904 24.253169997850847 23.792694478273674 17.190024755036575 14-15 22.697604075459683 29.69155456499244 34.99164212369657 12.619199235851308 16-17 15.400657481035731 31.71311220956611 33.10647849654942 19.77975181284874 18-19 14.537074993731618 39.690202617994835 28.2809508837424 17.491771504531144 20-21 20.843979591024507 28.169400864436327 23.983730130302234 27.00288941423693 22-23 15.96222269981135 29.499088600743445 30.035182399248594 24.503506300196605 24-25 26.428986476267802 29.99896522355151 25.04198804435211 18.530060255828577 26-27 22.003414775870507 22.711045486565762 38.44607798265549 16.839461754908243 28-29 14.660569372639387 39.99498525425955 28.506839555996006 16.837605817105057 30-31 18.461618063211688 28.813815666405308 27.109145834245435 25.61542043613757 32-33 19.119601990049752 30.152039800995023 31.8244776119403 18.903880597014926 34-35 25.38933928734891 27.095944821877026 28.50484957752757 19.00986631324649 36-37 19.972994933562756 25.248940509192874 37.84931332249945 16.928751234744926 38-39 18.607939270593953 30.41727569987375 33.704262767792066 17.270522261740233 40-41 17.85789677023631 33.291379729239935 30.357149969530532 18.493573530993224 42-43 16.29651167422112 33.44738037010189 24.366102937076306 25.890005018600686 44-45 20.338969548505307 25.160223855330504 28.15996494791978 26.340841648244407 46-47 25.653413843371865 31.898783586785946 29.19152732747564 13.25627524236655 48-49 21.768238770779732 28.445233903921363 30.846801494308725 18.939725830990177 50-51 10.481944024085255 31.447026777322908 39.77372286028322 18.297306338308616 52-53 18.6240234452771 35.624636653951214 25.23074962769473 20.52059027307695 54-55 14.383147132129862 26.669639515531806 31.394080314697288 27.553133037641043 56-57 22.581043409000397 24.77937076861808 28.930704898446834 23.70888092393469 58-59 26.88705896495762 25.345915729999398 29.502113762497245 18.264911542545732 60-61 19.846258286079387 29.63301653222586 35.89130261161249 14.629422570082262 62-63 15.52306051295114 30.217868912486267 34.150573925779696 20.108496648782896 64-65 14.45651265437381 38.37823423825008 28.652336054318884 18.51291705305723 66-67 19.58857473984113 28.01868797836637 25.00945666433268 27.38328061745982 68-69 17.67317287936601 28.771646609920754 29.542526823859372 24.012653686853863 70-71 21.103205457346146 29.434705005012347 28.03450777117615 21.42758176646536 72-73 22.60478010464838 23.705979386457315 33.77686630378834 19.912374205105966 74-75 15.793420416061926 36.973068859861314 27.88219641993227 19.35131430414449 76-77 17.183033083002208 27.77753274820271 27.89501329174068 27.144420877054404 78-79 18.86877031202472 28.68778316763524 31.04597921969806 21.39746730064198 80-81 23.617454180586027 26.989954170631776 27.88066048441363 21.511931164368562 82-83 18.68050083180107 25.454306659980418 35.33005388797351 20.535138620245004 84-85 17.47997086671522 29.935644096330876 31.530014845239375 21.054370191714526 86-87 17.673069116207245 32.06783465601095 28.713911596844678 21.545184630937126 88-89 16.384491088982813 31.5730193980785 24.160040117486925 27.882449395451758 90-91 19.96973254430309 24.793758036049418 27.78177084581167 27.45473857383582 92-93 22.63422832155857 31.013691647219062 28.796664303749075 17.555415727473296 94-95 19.684979271780698 28.42095960407494 29.789986181187132 22.104074942957226 96-97 14.485136135759461 30.580675964927188 32.153044998239885 22.781142901073466 98-99 17.835440959106585 32.98471053358916 25.24624572900352 23.933602778300735 100-101 14.789690639132349 27.81406331292272 29.318445591707686 28.077800456237245 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4597.0 1 3044.5 2 1243.5 3 929.5 4 755.5 5 679.0 6 822.0 7 1234.5 8 1381.5 9 1127.0 10 899.0 11 704.5 12 566.5 13 469.5 14 391.0 15 316.0 16 273.5 17 249.0 18 237.0 19 228.0 20 221.5 21 196.0 22 169.0 23 176.0 24 183.5 25 204.5 26 239.0 27 275.5 28 314.0 29 338.0 30 391.0 31 442.5 32 466.0 33 483.5 34 476.5 35 487.0 36 518.5 37 514.5 38 499.0 39 523.0 40 586.5 41 708.5 42 1028.5 43 1607.0 44 2823.5 45 5108.0 46 9303.5 47 15752.5 48 20494.0 49 18442.0 50 11917.5 51 6421.0 52 3181.5 53 1476.0 54 661.0 55 326.5 56 192.5 57 128.5 58 93.5 59 74.5 60 70.0 61 59.0 62 57.0 63 49.5 64 34.0 65 23.5 66 14.5 67 7.0 68 4.0 69 4.0 70 2.5 71 3.0 72 3.0 73 0.5 74 0.5 75 1.5 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023083474620117648 2 0.012735710135237322 3 0.0 4 0.0 5 0.058902659375472614 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.959818834523326E-4 16-17 0.0 18-19 3.979909417261663E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.979909417261663E-4 28-29 0.0011939728251784988 30-31 0.00437790035898783 32-33 0.004775891300713995 34-35 0.0011939728251784988 36-37 0.07880220646178093 38-39 0.07203636045243611 40-41 0.07601626986969777 42-43 0.07800622457832859 44-45 0.08238412493731642 46-47 0.07800622457832859 48-49 0.07004640574380527 50-51 0.06129060502582961 52-53 0.04935087677404462 54-55 0.03979909417261663 56-57 0.06447453255963895 58-59 0.6793705375265658 60-61 0.33510837293343204 62-63 0.003979909417261664 64-65 0.001989954708630832 66-67 1.098454999164219 68-69 2.3712300308044987 70-71 2.335410846049144 72-73 1.8032969569612596 74-75 1.282326814241708 76-77 0.7398651606689431 78-79 0.5611672278338945 80-81 0.04497297641505679 82-83 0.0 84-85 0.0011939728251784988 86-87 0.0015919637669046653 88-89 0.0 90-91 0.3295364997492657 92-93 0.06367855067618662 94-95 0.9257269304550628 96-97 1.639324688970079 98-99 0.6419593890043063 100-101 0.5540033908828235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 125631.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.00101885681082 #Duplication Level Percentage of deduplicated Percentage of total 1 79.88989852092591 9.587601786183345 2 9.265769052198713 2.223973382365817 3 3.2367181800092855 1.165317477374215 4 1.6846852822179479 0.80871759358757 5 1.0214233600848976 0.6129060502582961 6 0.6433640644690588 0.4632614561692576 7 0.45765072627180475 0.3844592497074767 8 0.3846919148371692 0.36933559392188237 9 0.3647940571731777 0.3940110323089046 >10 2.162233866153744 5.154778677237306 >50 0.31173310340253363 2.6219643240919837 >100 0.3316309610665252 7.959022852639874 >500 0.07959143065596604 6.315320263310807 >1k 0.14591762286927107 43.17326137657107 >5k 0.013265238442661007 9.632176771656678 >10k+ 0.006632619221330504 9.133892112615518 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11475 9.133892112615518 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6830 5.4365562639794325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5271 4.195620507677245 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4959 3.9472741600401173 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4859 3.8676759716948848 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3720 2.9610526064426774 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3536 2.814591939887448 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3223 2.565449610366868 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3143 2.5017710596906815 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3087 2.4571960742173506 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3067 2.4412764365483044 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2868 2.28287604174129 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2660 2.1173118099832045 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2128 1.6938494479865638 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2107 1.6771338284340649 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2083 1.658030263231209 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1960 1.560124491566572 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1916 1.5251012886946693 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1685 1.3412294736171804 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1568 1.2480995932532575 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1362 1.084127325262077 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1242 0.9886094992477971 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1054 0.8389649051587585 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.8023497385199512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1004 0.7991658109861419 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 988 0.7864301008509047 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 962 0.7657345718811439 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 768 0.6113140864913914 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 676 0.5380837532137768 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 654 0.5205721517778256 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 631 0.5022645684584219 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 611 0.4863449307893753 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 565 0.4497297641505679 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 547 0.4354020902484259 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 514 0.409134688094499 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 512 0.4075427243275943 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 506 0.4027668330268803 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 479 0.38127532217366733 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 429 0.3414762280010507 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 369 0.29371731499391074 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 363 0.28894142369319675 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 333 0.26506196718962677 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 325 0.2586941121220081 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 0.25073429328748476 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 307 0.24436643821986612 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 296 0.23561063750189046 RNA PCR Primer, Index 17 (95% over 21bp) CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 295 0.23481465561843812 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 264 0.21013921723141582 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 252 0.2005874346299878 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.1902396701451075 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 228 0.18148386942713185 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 221 0.1759119962429655 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 221 0.1759119962429655 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 218 0.17352405059260853 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 215 0.1711361049422515 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 207 0.16476824987463284 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 200 0.15919637669046652 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198 0.15760441292356187 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 190 0.1512365578559432 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 190 0.1512365578559432 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 187 0.1488486122055862 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 183 0.14566468467177687 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 167 0.13292897453653954 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 167 0.13292897453653954 RNA PCR Primer, Index 17 (95% over 23bp) TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 162 0.1289490651192779 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 161 0.12815308323582555 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 156 0.1241731738185639 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 153 0.12178522816820689 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 145 0.11541737310058822 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 144 0.1146213912171359 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 143 0.11382540933368357 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 142 0.11302942745023124 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 140 0.11143746368332656 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 131 0.10427362673225557 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 129 0.1026816629653509 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 127 0.10108969919844625 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 126 0.1002937173149939 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.979909417261663E-4 0.0 20-21 0.0 0.0 0.0 0.0023879456503569977 0.0 22-23 0.0 0.0 0.0 0.007561827892797159 0.0 24-25 0.0 0.0 0.0 0.015521646727320485 0.0 26-27 0.0 0.0 0.0 0.02905333874601014 0.0 28-29 0.0 0.0 0.0 0.0720363604524361 0.0 30-31 0.0 0.0 0.0 0.15840039480701418 0.0 32-33 0.0 0.0 0.0 0.34943604683557405 0.0 34-35 0.0 0.0 0.0 0.581464765861929 0.0 36-37 0.0 0.0 0.0 0.8325970500911399 0.0 38-39 0.0 0.0 0.0 1.1696953777332029 0.0 40-41 0.0 0.0 0.0 1.5274892343450261 0.0 42-43 0.0 0.0 0.0 1.8415040873669715 0.0 44-45 0.0 0.0 0.0 2.2056657990464137 0.0 46-47 0.0 0.0 0.0 2.636689988935852 0.0 48-49 0.0 0.0 0.0 2.9359791771139285 0.0 50-51 0.0 0.0 0.0 3.2324824286999227 0.0 52-53 0.0 0.0 0.0 3.5094841241413346 0.0 54-55 0.0 0.0 0.0 3.861706107568992 0.0 56-57 0.0 0.0 0.0 4.307853953244024 0.0 58-59 0.0 0.0 0.0 4.512819288233 0.0 60-61 0.0 0.0 0.0 4.70067101272775 0.0 62-63 0.0 0.0 0.0 4.873399081436906 0.0 64-65 0.0 0.0 0.0 5.0337894309525515 0.0 66-67 0.0 0.0 0.0 5.202537590244446 0.0 68-69 0.0 0.0 0.0 5.428596445144908 0.0 70-71 0.0 0.0 0.0 5.674554847131679 0.0 72-73 0.0 0.0 0.0 5.935238913962318 0.0 74-75 0.0 0.0 0.0 6.448647228789072 0.0 76-77 0.0 0.0 0.0 6.895193065405831 0.0 78-79 0.0 0.0 0.0 7.825297896219881 0.0 80-81 0.0 0.0 0.0 8.797589766856907 0.0 82-83 0.0 0.0 0.0 9.40969983523175 0.0 84-85 0.0 0.0 0.0 10.15712682379349 0.0 86-87 0.0 0.0 0.0 10.736203644005062 0.0 88-89 0.0 0.0 0.0 11.335975993186395 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGAG 25 0.0051537603 56.596577 5 CGCAGAA 300 0.0 48.73594 9 AACGCTA 20 4.788338E-4 48.635265 64-65 CTTAGTA 20 4.788338E-4 48.635265 68-69 ACGGTAA 20 4.788338E-4 48.635265 68-69 GCATTAG 20 4.788338E-4 48.635265 64-65 TCATAGG 40 3.4087861E-9 48.635265 66-67 TGATGAT 15 0.009396414 48.63526 66-67 CAGTTCT 15 0.009396414 48.63526 66-67 GGACCAC 15 0.009396414 48.63526 68-69 CCGCTTC 15 0.009396414 48.63526 64-65 GCAATGT 15 0.009396414 48.63526 64-65 ATAGTAT 15 0.009396414 48.63526 68-69 TAATCTT 15 0.009396414 48.63526 64-65 GTTGGTG 15 0.009396414 48.63526 66-67 AGTTCTT 15 0.009396414 48.63526 66-67 TGCAGAC 15 0.009396414 48.63526 64-65 TAGATAC 15 0.009396414 48.63526 68-69 GCTTAGA 15 0.009396414 48.63526 66-67 TTAGATA 15 0.009396414 48.63526 68-69 >>END_MODULE