FastQCFastQC Report
Fri 27 May 2016
SRR939553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523567
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108370.7112913314609728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71970.47237830695991706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56070.36801794735643395No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21740.14269146023771848No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.11643728172111892No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15470.1015380354129487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100550.033.7907831
GGTATCA64450.030.8502311
GTACATG78600.029.099921
TACATGG78500.028.5324862
TATCAAC125450.027.3107622
ACATGGG78500.027.021233
ATCAACG127150.026.9082973
TCAACGC127400.026.892744
CAACGCA128950.026.5326865
CATGGGA56000.026.014634
AACGCAG131850.025.9456966
ACGCAGA142700.023.6737067
CGCAGAG143650.023.2544358
GAGTACT69600.022.19191612-13
GCAGAGT144050.021.5435629
AGTACTT73100.021.51885612-13
ATGGGAG23650.021.2687535
ATGGGAT21900.021.018175
GTACTTT78400.019.76118714-15
CATGGGG26950.018.6644154
Produced by FastQC (version 0.11.5)