##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939553_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1523567 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.649506716803398 31.0 31.0 33.0 27.0 34.0 2 30.81012453013225 31.0 31.0 34.0 27.0 34.0 3 30.85026520002074 31.0 31.0 34.0 27.0 34.0 4 34.47199762137143 37.0 35.0 37.0 30.0 37.0 5 34.47967959400538 37.0 35.0 37.0 30.0 37.0 6 34.600352987430156 37.0 35.0 37.0 30.0 37.0 7 34.436954200241935 37.0 35.0 37.0 30.0 37.0 8 34.44804987243751 37.0 35.0 37.0 30.0 37.0 9 35.77857028932761 38.0 35.0 39.0 30.0 39.0 10-11 35.687597591704204 38.0 35.0 39.0 30.0 39.0 12-13 35.54528583252328 38.0 35.0 39.0 30.0 39.0 14-15 36.28558540582725 38.0 35.5 40.0 29.5 41.0 16-17 36.05312106392433 38.0 35.0 40.0 27.5 41.0 18-19 36.0735786480017 38.5 35.0 40.0 28.5 41.0 20-21 36.21471356363061 39.0 35.0 40.0 29.0 41.0 22-23 36.074431252449024 39.0 35.0 40.0 27.0 41.0 24-25 35.958302129148244 39.0 35.0 40.0 27.0 41.0 26-27 36.07534030338016 39.0 35.0 40.0 29.0 41.0 28-29 35.96271676926581 39.0 35.0 40.0 27.0 41.0 30-31 35.72488377603348 38.0 35.0 40.0 27.0 41.0 32-33 35.45992857550735 38.0 34.0 40.0 27.0 41.0 34-35 35.234136404897185 38.0 34.0 40.0 26.0 41.0 36-37 34.94468835305569 38.0 34.0 40.0 25.0 41.0 38-39 34.774105109916405 38.0 34.0 40.0 24.5 41.0 40-41 34.49926652388769 38.0 33.0 40.0 24.0 41.0 42-43 34.17123172134865 38.0 33.0 40.0 23.5 41.0 44-45 33.87148710887017 38.0 33.0 40.0 22.5 41.0 46-47 33.35150866355073 38.0 32.5 40.0 19.0 41.0 48-49 32.95776326213419 37.0 31.5 40.0 16.0 41.0 50-51 32.3320310166865 36.5 30.5 39.5 14.5 40.5 52-53 32.37451782560268 37.0 31.0 39.0 13.0 40.0 54-55 32.43729648909434 37.0 31.0 40.0 9.5 41.0 56-57 32.319709930708655 37.0 31.0 39.5 9.0 41.0 58-59 31.942259513365677 36.0 31.0 39.0 8.0 40.5 60-61 31.544284235612874 36.0 30.0 39.0 7.0 40.0 62-63 31.148488711031415 35.5 29.5 39.0 3.5 40.0 64-65 30.541877055620134 35.0 28.5 38.0 2.0 40.0 66-67 30.028992161158648 34.5 28.0 38.0 2.0 40.0 68-69 29.52364024686804 34.0 28.0 37.5 2.0 39.5 70-71 28.98361050088378 34.0 26.5 37.0 2.0 39.0 72-73 28.257513453625606 33.5 26.0 36.0 2.0 39.0 74-75 27.79930583952002 33.0 26.0 36.0 2.0 38.0 76-77 27.432166750789428 33.0 25.5 35.0 2.0 37.5 78-79 26.868797696458376 32.0 24.5 35.0 2.0 37.0 80-81 26.226104923511734 32.0 23.0 35.0 2.0 36.5 82-83 25.581193016125972 31.0 20.5 34.5 2.0 36.0 84-85 24.9441970717402 31.0 19.0 34.0 2.0 35.5 86-87 24.268573354502955 31.0 15.5 34.0 2.0 35.0 88-89 23.477547426532603 30.0 7.0 34.0 2.0 35.0 90-91 22.210927054734057 29.5 2.0 34.0 2.0 35.0 92-93 20.4669929185917 26.5 2.0 33.0 2.0 35.0 94-95 20.320314433169003 27.0 2.0 33.0 2.0 34.0 96-97 19.75658504023781 26.5 2.0 33.0 2.0 34.0 98-99 18.77893456605453 25.5 2.0 32.5 2.0 34.0 100-101 17.09662095595402 21.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9719845483563851 1101 2 0.8228593081650502 1101 3 0.8228472447659456 1101 4 0.8951573972925146 1101 5 0.619008253239798 1101 6 0.5781600159118341 1101 7 0.60581754692587 1101 8 0.760669314167636 1101 9 1.1272125274791733 1101 10-11 0.7862665681855887 1101 12-13 0.6991582931721823 1101 14-15 1.031923216994855 1101 16-17 0.7305320301603544 1101 18-19 0.927512442912402 1101 20-21 0.8870476260034508 1101 22-23 1.133742407269068 1101 24-25 0.893851436240304 1101 26-27 1.0328404163904992 1101 28-29 1.0437793483701157 1101 30-31 1.2489980349350986 1101 32-33 1.073048861827182 1101 34-35 1.112556917980207 1101 36-37 1.1075056011745517 1101 38-39 1.0539667763597222 1101 40-41 1.135512578216705 1101 42-43 1.1378696519087512 1101 44-45 1.2541211334794298 1101 46-47 1.1498517186170076 1101 48-49 0.7927133077345019 1101 50-51 0.9498279246876891 1101 52-53 1.198230398828116 1101 54-55 1.286193303924719 1101 56-57 0.9786986535230753 1101 58-59 0.9298423251096999 1101 60-61 0.6429550993178168 1101 62-63 0.9261288519886222 1101 64-65 0.8742421900245532 1101 66-67 0.7152805920228182 1101 68-69 0.6740612642958261 1101 70-71 0.734220916432335 1101 72-73 0.9486322722681599 1101 74-75 0.4494318264305939 1101 76-77 0.010095451941346312 1101 78-79 0.02761884992524699 1101 80-81 0.02421506681765706 1101 82-83 0.07560210887621466 1101 84-85 -0.20495302040700736 1101 86-87 -0.62713942755029 1101 88-89 -1.394327733464788 1101 90-91 -4.774211997004439 1101 92-93 -10.223462976664525 1101 94-95 -8.627250070504292 1101 96-97 -5.267723904915517 1101 98-99 -3.8495995834147383 1101 100-101 -3.314977640560624 1102 1 0.804446436017507 1102 2 0.7205759753680887 1102 3 0.6889400887519308 1102 4 0.6577397224054877 1102 5 0.6643843221174492 1102 6 0.5737472573414095 1102 7 0.5346786160212247 1102 8 0.5010463390483224 1102 9 0.6147362145841129 1102 10-11 0.3799929639026942 1102 12-13 0.44941551478125774 1102 14-15 0.6306960782456841 1102 16-17 0.8299890015048774 1102 18-19 0.6337693881324782 1102 20-21 0.6349004053219858 1102 22-23 0.6473649772702572 1102 24-25 0.7136344519471507 1102 26-27 0.6429227462361595 1102 28-29 0.7322515608640288 1102 30-31 0.8014726629931275 1102 32-33 0.8099516202272312 1102 34-35 0.6924174835175236 1102 36-37 0.6742438418639409 1102 38-39 0.7618515297204098 1102 40-41 0.6002713246979496 1102 42-43 0.425921175739731 1102 44-45 0.40409884251753425 1102 46-47 0.4522988970828905 1102 48-49 0.4445554593281855 1102 50-51 0.43496302400789233 1102 52-53 0.6394054496098605 1102 54-55 0.4485119709725325 1102 56-57 0.5869716766318405 1102 58-59 0.6100590727684398 1102 60-61 0.3758670183093358 1102 62-63 0.15224250087236868 1102 64-65 0.1409731897008406 1102 66-67 0.19269407374801872 1102 68-69 0.010127330731410922 1102 70-71 0.2605748912279324 1102 72-73 0.24076644966067207 1102 74-75 0.009930964134785114 1102 76-77 -0.08135949002643272 1102 78-79 -0.2802437071273083 1102 80-81 -0.6109781972417387 1102 82-83 -0.7483265051465473 1102 84-85 -0.9851515945426748 1102 86-87 -1.4295664920909061 1102 88-89 -1.8464502021864497 1102 90-91 -5.920691539485997 1102 92-93 -11.367709636806392 1102 94-95 -9.463668248949357 1102 96-97 -5.909776727396165 1102 98-99 -4.55605743125313 1102 100-101 -3.7221793250190434 1103 1 0.8229886355060003 1103 2 0.7175566516965901 1103 3 0.7027224132759429 1103 4 0.8176217911750214 1103 5 0.8091697725295006 1103 6 0.6329615227577179 1103 7 0.7158380363110837 1103 8 0.6338255376842952 1103 9 0.7853812842061672 1103 10-11 0.6857794089154794 1103 12-13 0.5562640512973118 1103 14-15 0.7314526852363059 1103 16-17 0.821179680675094 1103 18-19 0.8693831988741678 1103 20-21 0.8035207520113801 1103 22-23 0.8311529852270709 1103 24-25 0.8056883024728663 1103 26-27 0.7248707428260985 1103 28-29 0.919556192861755 1103 30-31 0.7865891733653925 1103 32-33 0.993970517641273 1103 34-35 1.0731897199483313 1103 36-37 0.9608131797440222 1103 38-39 0.7674816803313789 1103 40-41 0.6981506938369151 1103 42-43 0.8990491950634052 1103 44-45 0.6224314654217693 1103 46-47 0.7781016819649622 1103 48-49 0.655925877060497 1103 50-51 0.2974772325898769 1103 52-53 0.47730328990540016 1103 54-55 0.6177894645786637 1103 56-57 0.49786610686770416 1103 58-59 0.5481629375027346 1103 60-61 0.0513962879882186 1103 62-63 0.24417202630572987 1103 64-65 0.2104531556002449 1103 66-67 0.16211010102849244 1103 68-69 0.1805059894412686 1103 70-71 0.5200413589744528 1103 72-73 0.5504780154464086 1103 74-75 0.2612097365132975 1103 76-77 -0.14490737294771705 1103 78-79 -0.07475209019068174 1103 80-81 -0.107585898293177 1103 82-83 0.01920333287561604 1103 84-85 -0.35857088979701146 1103 86-87 -0.7001156537845716 1103 88-89 -1.0734500601006296 1103 90-91 -2.848511943009722 1103 92-93 -9.836557320807529 1103 94-95 -6.108098484811819 1103 96-97 -3.0988219106868726 1103 98-99 -2.172372435515708 1103 100-101 -1.800877108480254 1104 1 0.6044809974331429 1104 2 0.5773914865314254 1104 3 0.5287636719258977 1104 4 0.5352513772828686 1104 5 0.5495275215829025 1104 6 0.4474967101189975 1104 7 0.6395356469652143 1104 8 0.5855228872077376 1104 9 0.6761894837100186 1104 10-11 0.31154158413417576 1104 12-13 0.3325827613116772 1104 14-15 0.5183912542400932 1104 16-17 0.4584103896449321 1104 18-19 0.4123718396889018 1104 20-21 0.3527471523247385 1104 22-23 0.5218502041851565 1104 24-25 0.42267648630888033 1104 26-27 0.394517563559873 1104 28-29 0.4121433452315202 1104 30-31 0.4317288782877071 1104 32-33 0.602383822250232 1104 34-35 0.544000204345771 1104 36-37 0.5829032045514353 1104 38-39 0.2820903107008732 1104 40-41 0.22279327119687764 1104 42-43 0.1718964988889624 1104 44-45 0.2562625791441917 1104 46-47 0.023786497214992153 1104 48-49 0.028009373600518472 1104 50-51 0.22568097981535828 1104 52-53 0.2689258907671288 1104 54-55 0.18630199885207332 1104 56-57 0.1502179151977785 1104 58-59 0.17230936654047113 1104 60-61 -0.031331548543963805 1104 62-63 -0.25591936943784077 1104 64-65 -0.06930268719908028 1104 66-67 0.04018817910657191 1104 68-69 -0.09073536060442677 1104 70-71 0.18233898268511695 1104 72-73 0.288012288850247 1104 74-75 -0.0179161284821312 1104 76-77 -0.14195986021759666 1104 78-79 -0.36614130549293833 1104 80-81 -0.5952491049781194 1104 82-83 -0.672957850046437 1104 84-85 -0.6266166529514692 1104 86-87 -0.6771181998088522 1104 88-89 -0.6366518488458972 1104 90-91 -1.4182730084229647 1104 92-93 -4.989909586116315 1104 94-95 -2.5251666399234516 1104 96-97 -1.643811465459038 1104 98-99 -1.59726308703679 1104 100-101 -1.390528172587839 1105 1 0.9271654796681865 1105 2 0.8338175755417829 1105 3 0.8418284220692982 1105 4 0.7769728740605473 1105 5 0.6673633349243318 1105 6 0.6166028949555127 1105 7 0.6985227239298197 1105 8 0.6647515540768509 1105 9 0.7628008141415705 1105 10-11 0.655901674561683 1105 12-13 0.6031280256771581 1105 14-15 0.8247633566796182 1105 16-17 0.8171629624424455 1105 18-19 0.7108585286250246 1105 20-21 0.6476083056128559 1105 22-23 0.645406906955337 1105 24-25 0.6888269063918884 1105 26-27 0.6051630404974873 1105 28-29 0.6755202052375893 1105 30-31 0.6595943842430998 1105 32-33 0.5947514707976538 1105 34-35 0.405026961439944 1105 36-37 0.5715002285417228 1105 38-39 0.59679490293356 1105 40-41 0.6708851833337306 1105 42-43 0.8205569570999884 1105 44-45 0.5356818860915809 1105 46-47 0.5909036143321131 1105 48-49 0.41490582060932724 1105 50-51 0.36566984508512235 1105 52-53 0.4093820772907364 1105 54-55 0.6037818382420213 1105 56-57 0.4544862284548472 1105 58-59 0.3972747813935307 1105 60-61 0.3609787392719426 1105 62-63 0.3517956489513381 1105 64-65 0.3910154961528143 1105 66-67 0.3191013619523382 1105 68-69 0.1903069176288632 1105 70-71 0.45396566554775575 1105 72-73 0.20218376369588142 1105 74-75 0.1984552991252535 1105 76-77 -0.24006695607749862 1105 78-79 -0.05042109089631808 1105 80-81 -0.037825951487551635 1105 82-83 -0.058705395344542666 1105 84-85 -0.11588850907725856 1105 86-87 0.029444542708944255 1105 88-89 -0.05292205163295449 1105 90-91 -1.1397647248584981 1105 92-93 -7.585627776216526 1105 94-95 -3.1502265312754787 1105 96-97 -1.2480443500290015 1105 98-99 -0.9066685599254107 1105 100-101 -0.859170410331199 1106 1 0.6638374967896432 1106 2 0.6117630009029398 1106 3 0.5273393905016164 1106 4 0.6032222052537008 1106 5 0.31821049026586934 1106 6 0.4241934068156894 1106 7 0.55672140415097 1106 8 0.4495812312660803 1106 9 0.5518823194028499 1106 10-11 0.5152738878664849 1106 12-13 0.4650666737955902 1106 14-15 0.3687387445875814 1106 16-17 0.4555275067620457 1106 18-19 0.4899007972178566 1106 20-21 0.4681825877601682 1106 22-23 0.5149003972353441 1106 24-25 0.20532770896010533 1106 26-27 0.14508813413044663 1106 28-29 0.3665920796802524 1106 30-31 0.5002917268505556 1106 32-33 0.44027314013954566 1106 34-35 0.5105896509352235 1106 36-37 0.6284201500683793 1106 38-39 0.48309417170474234 1106 40-41 0.3230592514628583 1106 42-43 0.620879767872232 1106 44-45 0.4580129008945093 1106 46-47 0.49518932861781906 1106 48-49 0.5405790861702329 1106 50-51 0.2599323740667572 1106 52-53 0.4258971477383895 1106 54-55 0.26181465436472706 1106 56-57 0.16961731459716844 1106 58-59 0.15461738884849652 1106 60-61 0.12103224381982614 1106 62-63 0.3094860359675664 1106 64-65 0.38197144407505235 1106 66-67 0.2738561327745259 1106 68-69 0.15387210400303886 1106 70-71 0.33589539624153275 1106 72-73 0.4920963729343306 1106 74-75 0.3775278869618859 1106 76-77 0.0908586726009375 1106 78-79 0.12046530111366494 1106 80-81 0.17440340467438986 1106 82-83 -0.2131551902437785 1106 84-85 -0.3051323114671298 1106 86-87 -0.38431682700748127 1106 88-89 -0.34990796210201225 1106 90-91 -0.7698646000145537 1106 92-93 -4.489583545790275 1106 94-95 -2.1060246407814525 1106 96-97 -1.175857797505369 1106 98-99 -1.0390477288214335 1106 100-101 -0.8298160318756977 1107 1 0.6079009493925618 1107 2 0.5651932099453312 1107 3 0.5425222409361758 1107 4 0.6518111937805386 1107 5 0.4145357243163943 1107 6 0.5188498531937 1107 7 0.502319873080701 1107 8 0.4512362077377077 1107 9 0.40694086902945514 1107 10-11 0.36055884253615034 1107 12-13 0.36157303188056034 1107 14-15 0.45282015175165213 1107 16-17 0.5298331486376142 1107 18-19 0.37987445728474256 1107 20-21 0.4533576447313763 1107 22-23 0.3647825087706593 1107 24-25 0.4619682329965258 1107 26-27 0.3402486893407186 1107 28-29 0.3445677522364079 1107 30-31 0.3608998142951876 1107 32-33 0.4490576558103285 1107 34-35 0.4115797167357229 1107 36-37 0.3642967173930458 1107 38-39 0.26767514973506223 1107 40-41 0.31969582944189057 1107 42-43 0.2548488110519642 1107 44-45 0.38325918420170524 1107 46-47 0.4128254429351088 1107 48-49 0.3995627790880434 1107 50-51 0.36107199544255053 1107 52-53 0.7037084670040272 1107 54-55 0.6271846465712656 1107 56-57 0.5175615173456052 1107 58-59 0.44830251319862313 1107 60-61 0.2790302116002721 1107 62-63 0.31375645928087437 1107 64-65 0.32192981316952896 1107 66-67 0.3876124834606465 1107 68-69 0.4430563552879718 1107 70-71 0.46306321219741164 1107 72-73 0.4296476470176138 1107 74-75 0.48325894342010045 1107 76-77 0.26679364157506313 1107 78-79 0.29553746463828645 1107 80-81 0.07445154657461117 1107 82-83 0.1468238956520267 1107 84-85 0.01404986813767195 1107 86-87 0.047922067307236915 1107 88-89 0.24250574255532342 1107 90-91 0.013110332655841717 1107 92-93 -3.940895313919942 1107 94-95 -1.6197227871209918 1107 96-97 -0.513285165386435 1107 98-99 -0.34253900310201857 1107 100-101 -0.045601642577405954 1108 1 0.45986586558397136 1108 2 0.38098961353380645 1108 3 0.14203712775254473 1108 4 -0.0780476360161444 1108 5 -0.8152579325642577 1108 6 -0.2289237833227773 1108 7 0.19746439638332447 1108 8 0.182468769259998 1108 9 0.36767428157779136 1108 10-11 0.3358074913788087 1108 12-13 0.36509369169494477 1108 14-15 0.46804303367910194 1108 16-17 0.5030519917333436 1108 18-19 0.48803656216212943 1108 20-21 0.41442372251194115 1108 22-23 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0.3562287581945256 2311 46-47 0.5438395329239754 2311 48-49 0.5870135510452883 2311 50-51 0.48089516677459443 2311 52-53 0.5521062865094066 2311 54-55 0.3815109454224057 2311 56-57 0.31585768863457275 2311 58-59 0.5623163908915565 2311 60-61 0.49605896077724054 2311 62-63 0.41620770813478103 2311 64-65 0.5433560111031817 2311 66-67 0.5226711571341056 2311 68-69 0.6546309834624111 2311 70-71 0.7347675870320387 2311 72-73 0.6607102343204723 2311 74-75 0.7696175066381379 2311 76-77 0.8045353974162737 2311 78-79 0.8102191514987034 2311 80-81 0.7352612940347534 2311 82-83 0.8143721160582835 2311 84-85 0.7675531356490133 2311 86-87 0.8737890192914364 2311 88-89 0.8800523553583055 2311 90-91 1.8834109775432033 2311 92-93 2.8801822879359555 2311 94-95 2.077905305784629 2311 96-97 1.7073733827555166 2311 98-99 2.0486958669667175 2311 100-101 2.2680352175349547 2312 1 0.12533946379099348 2312 2 -0.06818454572771415 2312 3 0.13091835435184862 2312 4 -0.0041353584047314484 2312 5 0.05559721643187743 2312 6 -0.15693003475164602 2312 7 0.12887257945874353 2312 8 -0.6469380570904448 2312 9 0.0431801488511141 2312 10-11 -0.10415049512140229 2312 12-13 0.00424307760824405 2312 14-15 -0.36932087218966814 2312 16-17 -0.1671838235571741 2312 18-19 -0.1963166885434049 2312 20-21 0.05891830498170947 2312 22-23 0.00981653056085463 2312 24-25 0.06430584686548713 2312 26-27 0.008285832445281471 2312 28-29 -0.05162881163558808 2312 30-31 0.0365988530713679 2312 32-33 0.012584219935213525 2312 34-35 -0.06926688518518631 2312 36-37 -0.0790906635460118 2312 38-39 0.024126798483358414 2312 40-41 0.18807970436247246 2312 42-43 0.13943240717703276 2312 44-45 -0.26328434138916634 2312 46-47 0.012468212222231045 2312 48-49 -0.2288971630224239 2312 50-51 -0.12974664262829094 2312 52-53 -0.25620509718753226 2312 54-55 -0.07997698049719304 2312 56-57 -0.09996481994181039 2312 58-59 -0.2614294277408611 2312 60-61 -0.021406642133236176 2312 62-63 0.11156303281644853 2312 64-65 0.14619628896574355 2312 66-67 -0.052953606328678404 2312 68-69 -0.14556793670704238 2312 70-71 -0.3843063926532295 2312 72-73 -0.0941181770040167 2312 74-75 -0.03661769071020515 2312 76-77 0.2261805366527625 2312 78-79 0.3347247887147091 2312 80-81 0.3786871617448284 2312 82-83 0.39571001634029557 2312 84-85 0.28022008514297525 2312 86-87 0.29174036282282856 2312 88-89 0.6997964983915956 2312 90-91 1.2137687744202985 2312 92-93 2.0822857905180285 2312 94-95 1.5910197684331173 2312 96-97 1.2483050036611338 2312 98-99 1.4270630887073885 2312 100-101 1.3906660302463116 2313 1 -0.2283500224612638 2313 2 -1.0686838255206013 2313 3 -0.42811390422722795 2313 4 -0.1880457879289139 2313 5 -0.9242298062494569 2313 6 -0.36316608721909205 2313 7 -1.068926761109097 2313 8 -1.0840391716169364 2313 9 -0.9800004707981813 2313 10-11 -1.9529903475387584 2313 12-13 -0.5014671359335594 2313 14-15 -0.2993291828852591 2313 16-17 -1.5502307223597 2313 18-19 -1.1381537829583337 2313 20-21 -1.3349955062458676 2313 22-23 -1.0908820623634128 2313 24-25 -0.14809104707185128 2313 26-27 -0.8476328663007777 2313 28-29 -2.040184856191125 2313 30-31 -0.5546381485062355 2313 32-33 -0.27984698553038356 2313 34-35 -0.040216936469043674 2313 36-37 0.0854870302917945 2313 38-39 -0.46469962282903055 2313 40-41 -0.26636619875171874 2313 42-43 0.06783720026304252 2313 44-45 -0.3126460448743984 2313 46-47 -0.6896770957440737 2313 48-49 -0.21705043394312185 2313 50-51 -0.07276254780023095 2313 52-53 0.08430491791847317 2313 54-55 0.31708006022522284 2313 56-57 -0.11660375770410525 2313 58-59 -0.1650337333511409 2313 60-61 -0.25425965129095474 2313 62-63 -0.06920017935513201 2313 64-65 0.11305360181956203 2313 66-67 0.04233864781590313 2313 68-69 -0.08937662800378021 2313 70-71 -0.4506938561045146 2313 72-73 -0.016390043624529227 2313 74-75 0.09173310678159297 2313 76-77 0.41206136670143323 2313 78-79 0.4788942216253851 2313 80-81 0.32651042945929376 2313 82-83 0.3762408959181549 2313 84-85 0.5936413799844402 2313 86-87 0.5563897583433466 2313 88-89 0.4453013851125114 2313 90-91 1.1624294922666003 2313 92-93 2.2898597692675295 2313 94-95 1.3849324630605153 2313 96-97 1.3769102264786959 2313 98-99 1.2660509385023317 2313 100-101 1.0918275337005987 2314 1 -0.6300317818403087 2314 2 -0.7834315616137459 2314 3 -1.8116844087550419 2314 4 -0.5677353092743189 2314 5 -0.2528197156931853 2314 6 -2.7525580665205176 2314 7 -3.722872427344541 2314 8 -1.54199518714087 2314 9 -1.6604661887800702 2314 10-11 -1.059717385054931 2314 12-13 -2.0826443674988937 2314 14-15 -2.830700463609709 2314 16-17 -2.1349655218422328 2314 18-19 -2.516873058508139 2314 20-21 -2.214038197060873 2314 22-23 -3.2519428644332677 2314 24-25 -1.7719590936436518 2314 26-27 -1.825640628267145 2314 28-29 -1.1879856323877576 2314 30-31 -1.4873677733445234 2314 32-33 -2.0204679428395664 2314 34-35 -0.96712508653372 2314 36-37 -0.5703991275348557 2314 38-39 -0.5397319643555534 2314 40-41 -0.7016831457116197 2314 42-43 -0.7316453353126349 2314 44-45 -1.138001801666121 2314 46-47 -1.746274767154162 2314 48-49 -1.8716429307089726 2314 50-51 -1.1403563382271358 2314 52-53 -0.6886575659107628 2314 54-55 -0.2925577146777627 2314 56-57 -0.36692717296930866 2314 58-59 -0.7484748717728742 2314 60-61 -0.3752169604759459 2314 62-63 -0.4595624044521571 2314 64-65 -0.8610731769643927 2314 66-67 -0.6180753237235557 2314 68-69 -0.5311618802676854 2314 70-71 -0.8866576335948082 2314 72-73 -0.5856009103774404 2314 74-75 -0.37042730718994576 2314 76-77 -0.3828933551614391 2314 78-79 -0.31217951705508185 2314 80-81 -0.30026835449931255 2314 82-83 -0.11049907820862259 2314 84-85 -0.29769109090818446 2314 86-87 -0.15997505407580093 2314 88-89 -0.09544893830275569 2314 90-91 0.3467761934308946 2314 92-93 1.3904419167448907 2314 94-95 0.7199906778082514 2314 96-97 0.6275570570090956 2314 98-99 0.9280819863677898 2314 100-101 0.8486192542698063 2315 1 -7.155810619484978 2315 2 -2.7156762800303156 2315 3 -1.104142728450384 2315 4 -0.7080533732507988 2315 5 -0.9328126499138989 2315 6 -1.0856593374948815 2315 7 -2.805057138591426 2315 8 -2.304842176651306 2315 9 -2.8488367473403144 2315 10-11 -1.8691686441664075 2315 12-13 -2.3836768718154318 2315 14-15 -4.174537216399223 2315 16-17 -3.62547650930221 2315 18-19 -2.4377584110437667 2315 20-21 -3.6110141993884213 2315 22-23 -4.939392966696573 2315 24-25 -3.1873745285614135 2315 26-27 -3.94870035315202 2315 28-29 -2.227255692360295 2315 30-31 -1.8682548403707173 2315 32-33 -0.9887247562077519 2315 34-35 -1.6909938258289827 2315 36-37 -2.310631293802345 2315 38-39 -1.533268421525328 2315 40-41 -2.72791225360503 2315 42-43 -6.334708298679686 2315 44-45 -3.159307810522588 2315 46-47 -1.7588286480507591 2315 48-49 -4.815856178698773 2315 50-51 -5.724789370052584 2315 52-53 -6.103974720687695 2315 54-55 -3.354647384265988 2315 56-57 -2.836460329311798 2315 58-59 -3.055698159057414 2315 60-61 -1.7452213419518294 2315 62-63 -2.015373401783439 2315 64-65 -1.0386883379100311 2315 66-67 -1.8740344772847557 2315 68-69 -1.0326109711547353 2315 70-71 -1.0017523375507835 2315 72-73 -1.2945033593280506 2315 74-75 -1.6150270948702037 2315 76-77 -1.8914515205526286 2315 78-79 -0.8425333601982459 2315 80-81 -0.5796298712865955 2315 82-83 -1.0915258156023562 2315 84-85 -1.080905881939497 2315 86-87 -1.4834046066976718 2315 88-89 -1.0790339900232233 2315 90-91 0.028154141618646378 2315 92-93 0.9780125528901635 2315 94-95 0.6978135692013332 2315 96-97 0.5908279932768075 2315 98-99 0.40793066092804153 2315 100-101 0.2187630463419339 2316 1 -4.17886035103276 2316 2 -2.995600722759715 2316 3 -4.3553268242642424 2316 4 -4.218057803784319 2316 5 -3.827651792962964 2316 6 -3.9882700174626784 2316 7 -2.4314207186530723 2316 8 -1.1712830290287854 2316 9 -1.6288621871779512 2316 10-11 -2.821606866807869 2316 12-13 -3.6433985580860124 2316 14-15 -2.6899337967895818 2316 16-17 -2.7421870049026396 2316 18-19 -2.8964608364035698 2316 20-21 -5.251556439523338 2316 22-23 -6.239050910452612 2316 24-25 -4.151734935789378 2316 26-27 -3.7371657211483225 2316 28-29 -4.2304287642445075 2316 30-31 -5.935360987378672 2316 32-33 -4.0669663108009715 2316 34-35 -5.63137837708263 2316 36-37 -8.915798343972178 2316 38-39 -5.7456986882625 2316 40-41 -4.896471901449075 2316 42-43 -4.150494575459405 2316 44-45 -3.1071873476509353 2316 46-47 -5.3826630127932376 2316 48-49 -2.944491240892983 2316 50-51 -3.980756774665103 2316 52-53 -4.970538484640304 2316 54-55 -6.4708555145331985 2316 56-57 -4.446191305410736 2316 58-59 -5.266875013569173 2316 60-61 -5.775831564574229 2316 62-63 -6.082226103217884 2316 64-65 -4.6044805697079205 2316 66-67 -6.101709590549202 2316 68-69 -4.4343750857388144 2316 70-71 -5.5185993221677805 2316 72-73 -7.861711456247161 2316 74-75 -7.683401898950109 2316 76-77 -7.016185331240219 2316 78-79 -5.7606418558653765 2316 80-81 -5.8062993010816975 2316 82-83 -4.699654653611571 2316 84-85 -6.322798243794981 2316 86-87 -4.937599321499938 2316 88-89 -5.007892484499347 2316 90-91 -5.6275395703585644 2316 92-93 -4.500043919738193 2316 94-95 -4.280258386474275 2316 96-97 -5.1775637230547105 2316 98-99 -6.1678112152361635 2316 100-101 -5.235157499423572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6707.0 3 2263.0 4 2112.0 5 2562.0 6 2867.0 7 3375.0 8 4193.0 9 5258.0 10 6667.0 11 8179.0 12 10376.0 13 12396.0 14 13812.0 15 16283.0 16 19303.0 17 22646.0 18 19296.0 19 16696.0 20 16955.0 21 18586.0 22 20984.0 23 23592.0 24 26897.0 25 31253.0 26 35537.0 27 40893.0 28 47753.0 29 55873.0 30 64605.0 31 75780.0 32 89288.0 33 106275.0 34 127851.0 35 155586.0 36 184027.0 37 169446.0 38 56350.0 39 1045.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05080270988076 19.683356752137087 14.082616809905662 22.183223728076484 2 15.085686129588568 21.466173934466617 40.95248419150858 22.49565574443623 3 18.375349661605085 27.070804750536396 30.71733677750288 23.83650881035563 4 12.017096892023641 17.308632274916764 38.87059602614201 31.80367480691759 5 12.625994869217605 38.67243469092128 34.647496854850104 14.05407358501102 6 29.468539378242763 37.79931648611066 18.55386169583172 14.178282439814849 7 26.42122737601521 34.45303349115077 22.2295944812291 16.89614465160492 8 25.539329451943658 34.74627653948908 20.936775564981307 18.777618443585954 9 27.132552849501675 16.251562511289823 21.03391445944499 35.581970179763516 10-11 23.994250749941454 27.20997473864134 28.332037584277064 20.463736927140143 12-13 25.710187569200777 25.168832644733286 28.554747891583183 20.56623189448276 14-15 22.824988363915626 25.721149819225996 27.68044635264184 23.773415464216534 16-17 21.099838331523515 29.04741070953476 28.1162896263494 21.736461332592324 18-19 20.99769776204339 27.43361727272996 31.0932197794726 20.475465185754043 20-21 22.145598451070523 26.657604526952696 30.80175956181116 20.39503746016562 22-23 21.7345296482838 26.768592823095872 30.53605847492512 20.960819053695207 24-25 21.359658447352345 26.867894793740565 30.897758634683182 20.874688124223905 26-27 21.27697819267551 26.919272611601436 30.929615991030218 20.87413320469284 28-29 21.077339705767823 26.934412756436103 31.07912406629146 20.909123471504618 30-31 21.2695570963206 27.08761559434743 30.86921559986816 20.77361170946381 32-33 21.023617493349036 27.111319519658462 30.786371307795037 21.078691679197465 34-35 21.402709683736607 27.03765976064121 30.538010193268605 21.02162036235358 36-37 21.598676395444937 26.875580186020986 30.503827379358274 21.021916039175803 38-39 21.344301185500616 27.055042977683147 30.678048524038587 20.922607312777647 40-41 21.329230688353952 26.949153377700164 30.72764997626139 20.99396595768449 42-43 21.132443339883814 27.246731851661043 30.343320268516006 21.277504539939144 44-45 21.28295256565216 27.39483810898654 30.060644449533104 21.2615648758282 46-47 21.413219026276057 27.348292048178518 29.516851145069523 21.721637780475902 48-49 21.491459561498587 27.544006904126373 29.390223141837602 21.574310392537445 50-51 21.626218594116832 27.752837018244275 29.17224177275391 21.448702614884983 52-53 21.511609943247 27.897390992227606 29.131093321973527 21.459905742551864 54-55 21.478137625739713 27.821328978870604 29.10893256499154 21.591600830398146 56-57 21.39631720728672 28.249980119833047 28.765024312951287 21.588678359928945 58-59 21.364581906095907 27.819128645061703 29.01592164462437 21.80036780421802 60-61 21.55816232051293 27.842567226125947 29.083887349377402 21.515383103983723 62-63 21.695608447650987 27.618715622976314 29.06300648664313 21.622669442729574 64-65 21.470116434425275 27.640421533157845 29.15965704685662 21.729804985560268 66-67 21.313629320643283 27.94600814024242 28.841842212821213 21.898520326293088 68-69 21.303141630630552 27.901479315019255 28.976024622614666 21.819354431735523 70-71 21.33380253090691 27.800514803052017 28.805215321600762 22.060467344440305 72-73 21.377486882603204 27.764973058600624 28.584767222694207 22.272772836101968 74-75 21.362574383435927 27.993547080803488 28.450628857223247 22.193249678537338 76-77 21.434589059219636 27.792315624187484 28.71413305476767 22.058962261825208 78-79 21.196732061591863 27.86297141786519 28.714666771850762 22.22562974869218 80-81 21.192452011498503 28.07935873983239 28.4991625946909 22.22902665397821 82-83 21.333976737520018 28.02447083511935 28.33467887206162 22.306873555299013 84-85 21.313873458889816 27.714085896619334 28.28409208176486 22.68794856272599 86-87 21.26530484808947 27.69985654400878 28.560132380465664 22.47470622743608 88-89 21.53952406498677 27.425005653996454 28.410740489452007 22.624729791564764 90-91 20.893243750123386 27.645150464257746 28.86794811895001 22.59365766666886 92-93 20.609905948003473 27.687859822137987 29.06109024293026 22.641143986928277 94-95 21.15016333606601 27.57809002318721 28.342702667877195 22.929043972869582 96-97 21.275650761715028 27.359902952837444 28.147950897817754 23.216495387629774 98-99 21.19411681151491 27.264952599903204 28.130918436059847 23.410012152522043 100-101 21.294393943180488 27.29458861163249 28.32863323016782 23.082384215019196 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2889.0 1 1885.5 2 938.0 3 996.0 4 874.5 5 897.0 6 1301.0 7 2355.5 8 3696.0 9 4095.5 10 3418.0 11 2773.0 12 2736.5 13 2814.0 14 2909.5 15 2989.0 16 3077.0 17 3253.0 18 3492.5 19 3716.5 20 3987.5 21 4582.5 22 5323.0 23 6062.0 24 6987.5 25 8382.5 26 10337.5 27 12557.0 28 14644.5 29 17046.5 30 19670.5 31 22842.0 32 26755.5 33 30357.0 34 34234.0 35 37434.5 36 40751.0 37 44870.0 38 46710.0 39 46780.5 40 46807.5 41 47384.0 42 48541.0 43 50312.5 44 52414.0 45 53412.5 46 53444.5 47 54695.5 48 59484.5 49 60086.0 50 55042.5 51 51309.5 52 48453.0 53 47025.0 54 45244.5 55 41742.0 56 37699.5 57 33674.0 58 30562.5 59 27183.5 60 22812.0 61 18069.5 62 13765.0 63 10344.5 64 7536.5 65 5347.5 66 3623.0 67 2624.0 68 1869.5 69 1096.5 70 670.5 71 419.0 72 262.0 73 172.0 74 125.0 75 95.0 76 66.0 77 46.5 78 30.0 79 24.0 80 18.5 81 12.0 82 7.0 83 7.5 84 7.5 85 4.0 86 4.0 87 3.5 88 1.5 89 0.5 90 1.5 91 1.5 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08440718393086749 2 0.09464631355234132 3 0.09064255132855989 4 0.09313669828763683 5 0.09070818677485139 6 0.07482440877230867 7 0.08821403981577443 8 0.08270066232728852 9 0.07731855573138562 10-11 0.08411182442255576 12-13 0.08371801174480677 14-15 0.08942829557216715 16-17 0.08637624731961246 18-19 0.08877194110925217 20-21 0.09737018457343852 22-23 0.10603406348391635 24-25 0.126512322726864 26-27 0.13218978883107865 28-29 0.1321569711079329 30-31 0.13314150280230538 32-33 0.13015508999604217 34-35 0.12316491496599756 36-37 0.12894083423964944 38-39 0.12122866930039833 40-41 0.11712645390717967 42-43 0.11784844381638615 44-45 0.1091845649059083 46-47 0.1269717708509045 48-49 0.14164129309705448 50-51 0.15066616696213556 52-53 0.15844396734767818 54-55 0.15549037226456072 56-57 0.12798912026842274 58-59 0.12034259077546311 60-61 0.11814380332469789 62-63 0.11469794239439421 64-65 0.10183339492126044 66-67 0.11453385377866547 68-69 0.112499154943629 70-71 0.11945651225052788 72-73 0.14502151858106666 74-75 0.1571968938681397 76-77 0.1602489421206944 78-79 0.15913313953373892 80-81 0.16405579800560133 82-83 0.16349789671212359 84-85 0.14308527291546747 86-87 0.1440369868866942 88-89 0.16533568920828556 90-91 0.25840675204963093 92-93 0.5405079002104929 94-95 0.1621195523400021 96-97 0.1743933807965124 98-99 0.1900146170138891 100-101 0.19907230860211597 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1523567.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.99460404803995 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26396329496669 42.703704278359126 2 12.890033785639401 14.435446759854717 3 4.514623336544209 7.583836384675021 4 2.103372995243296 4.711101521359528 5 1.1848923798545552 3.3173789824747786 6 0.7151892190884157 2.402804228536964 7 0.4699420573546897 1.8419953598967993 8 0.32098979905978586 1.4378957361450095 9 0.23398904516813807 1.1791911542191944 >10 1.1945762618380076 11.670205004319593 >50 0.07363996437731234 2.833928586677291 >100 0.03217588141948445 3.043468950582499 >500 0.0014247160614696147 0.5541801402404983 >1k 8.310843691906086E-4 0.7132011209812938 >5k 2.3745267691160243E-4 0.851214313296466 >10k+ 1.1872633845580121E-4 0.7204474783812715 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10837 0.7112913314609728 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7197 0.47237830695991706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5607 0.36801794735643395 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2174 0.14269146023771848 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1774 0.11643728172111892 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1547 0.1015380354129487 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.563544629149884E-5 0.0 0.0 0.0 0.0 7 1.969063388744965E-4 0.0 0.0 0.0 0.0 8 1.969063388744965E-4 0.0 0.0 0.0 0.0 9 1.969063388744965E-4 0.0 0.0 0.0 0.0 10-11 1.969063388744965E-4 0.0 0.0 0.0 0.0 12-13 1.969063388744965E-4 0.0 0.0 6.563544629149884E-5 0.0 14-15 2.6254178516599535E-4 0.0 0.0 6.563544629149884E-5 0.0 16-17 2.6254178516599535E-4 0.0 0.0 2.953595083117447E-4 0.0 18-19 3.2817723145749415E-4 0.0 0.0 4.266304008947424E-4 0.0 20-21 3.93812677748993E-4 0.0 0.0 6.23536739769239E-4 0.0 22-23 4.594481240404918E-4 0.0 0.0 0.0013783443721214754 0.0 24-25 4.594481240404918E-4 0.0 0.0 0.002001881111890714 0.0 26-27 4.594481240404918E-4 0.0 0.0 0.0028879596368259486 0.0 28-29 4.594481240404918E-4 0.0 0.0 0.004791387579279415 0.0 30-31 4.594481240404918E-4 0.0 0.0 0.013356813320320012 0.0 32-33 4.594481240404918E-4 0.0 0.0 0.03183319145137693 0.0 34-35 4.922658471862413E-4 0.0 0.0 0.05743101550506148 0.0 36-37 5.250835703319907E-4 0.0 0.0 0.09970024291678672 0.0 38-39 5.907190166234894E-4 0.0 0.0 0.15808297239307495 0.0 40-41 5.907190166234894E-4 0.0 0.0 0.22634383653623372 0.0 42-43 5.907190166234894E-4 0.0 0.0 0.2996586300438379 3.281772314574942E-5 44-45 5.907190166234894E-4 0.0 0.0 0.3806855884906932 6.563544629149884E-5 46-47 7.548076323522366E-4 0.0 0.0 0.47812140851042323 6.563544629149884E-5 48-49 8.532608017894848E-4 0.0 0.0 0.5896032140365339 6.563544629149884E-5 50-51 8.532608017894848E-4 0.0 0.0 0.7096176275805396 6.563544629149884E-5 52-53 8.532608017894848E-4 0.0 0.0 0.8301243069717315 6.563544629149884E-5 54-55 8.532608017894848E-4 0.0 0.0 0.9519765130118991 6.563544629149884E-5 56-57 9.845316943724826E-4 0.0 0.0 1.0921410085673946 6.563544629149884E-5 58-59 9.845316943724826E-4 0.0 0.0 1.2613491891068789 6.563544629149884E-5 60-61 9.845316943724826E-4 0.0 0.0 1.4541861303113024 6.563544629149884E-5 62-63 9.845316943724826E-4 0.0 0.0 1.6469246183462887 6.563544629149884E-5 64-65 9.845316943724826E-4 0.0 0.0 1.8429776964189957 6.563544629149884E-5 66-67 0.0010501671406639814 0.0 0.0 2.0640378795287635 6.563544629149884E-5 68-69 0.0010501671406639814 0.0 0.0 2.315191914763184 6.563544629149884E-5 70-71 0.0010501671406639814 0.0 0.0 2.6010014656395155 6.563544629149884E-5 72-73 0.0010501671406639814 0.0 0.0 2.902333799563787 6.563544629149884E-5 74-75 0.0010501671406639814 0.0 0.0 3.21360990360122 6.563544629149884E-5 76-77 0.0010501671406639814 0.0 0.0 3.5352892258758555 6.563544629149884E-5 78-79 0.0010501671406639814 0.0 0.0 3.899992583194569 6.563544629149884E-5 80-81 0.0012470734795384777 0.0 0.0 4.301779967667979 6.563544629149884E-5 82-83 0.0012470734795384777 0.0 0.0 4.71013089677054 6.563544629149884E-5 84-85 0.0012470734795384777 0.0 0.0 5.110310212809807 6.563544629149884E-5 86-87 0.0012470734795384777 0.0 0.0 5.5491488067147685 6.563544629149884E-5 88-89 0.0012470734795384777 0.0 0.0 6.001147307601175 6.563544629149884E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10055 0.0 33.790783 1 GGTATCA 6445 0.0 30.850231 1 GTACATG 7860 0.0 29.09992 1 TACATGG 7850 0.0 28.532486 2 TATCAAC 12545 0.0 27.310762 2 ACATGGG 7850 0.0 27.02123 3 ATCAACG 12715 0.0 26.908297 3 TCAACGC 12740 0.0 26.89274 4 CAACGCA 12895 0.0 26.532686 5 CATGGGA 5600 0.0 26.01463 4 AACGCAG 13185 0.0 25.945696 6 ACGCAGA 14270 0.0 23.673706 7 CGCAGAG 14365 0.0 23.254435 8 GAGTACT 6960 0.0 22.191916 12-13 GCAGAGT 14405 0.0 21.543562 9 AGTACTT 7310 0.0 21.518856 12-13 ATGGGAG 2365 0.0 21.268753 5 ATGGGAT 2190 0.0 21.01817 5 GTACTTT 7840 0.0 19.761187 14-15 CATGGGG 2695 0.0 18.664415 4 >>END_MODULE