FastQCFastQC Report
Fri 27 May 2016
SRR939553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523567
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106130.6965889914916771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79190.5197670991823792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62890.4127813217272362No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19680.1291705583016697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18450.12109739840781533No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18000.11814380332469789No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.11610910448966144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.10429472415719164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64250.035.3992841
GTATCAA99550.031.9761331
ATCAACG122050.025.9218713
TATCAAC123200.025.7580262
TCAACGC123150.025.728564
CAACGCA125000.025.2347855
GTACATG73100.025.1871991
AACGCAG127450.024.863956
TACATGG73850.024.4818442
ACATGGG72400.023.5399133
ACGCAGA141500.022.096087
CGCAGAG140600.022.0034438
GAGTACT73450.021.92323112-13
AGTACTT75450.021.18631712-13
CATGGGA53600.020.8170134
GCAGAGT145600.020.6336149
CATGGGG23200.020.09014
GTACTTT81750.019.58236714-15
AGTACAT55900.018.6984062
AGAGTAC134500.017.98452210-11
Produced by FastQC (version 0.11.5)