FastQCFastQC Report
Fri 27 May 2016
SRR939552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474976
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45230.306649057340594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29620.200816826850064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23380.1585110537391795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55650.044.8330461
GTATCAA83950.038.074491
GTACATG60700.033.509771
TATCAAC112000.027.4587632
TACATGG71450.026.6453112
ATCAACG120600.025.5798873
ACATGGG70650.025.2093683
TCAACGC122950.025.2045274
CAACGCA125650.024.717125
GTGTACG2050.001399488324.438821
AACGCAG128500.024.1689196
ACGCAGA132400.023.1361527
GAGTACT66100.022.95576912-13
CATGGGA52600.022.8113634
CGCAGAG139300.021.7198128
AGTACTT70500.021.25528512-13
GCAGAGT138800.020.6064369
ACGCATA1150.003863367520.5213817
CGGGTCG1405.065014E-420.228226
GTACTTT75350.020.20411914-15
Produced by FastQC (version 0.11.5)