##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939552_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1474976 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.535582273881067 2.0 2.0 31.0 2.0 33.0 2 22.870639929056473 16.0 16.0 31.0 16.0 34.0 3 27.587926854403054 28.0 25.0 31.0 16.0 34.0 4 32.8666405419478 35.0 33.0 35.0 28.0 37.0 5 33.46266786713818 35.0 33.0 37.0 28.0 37.0 6 33.89038804699195 35.0 35.0 37.0 29.0 37.0 7 34.060161656867635 35.0 35.0 37.0 30.0 37.0 8 34.01168832577615 36.0 35.0 37.0 29.0 37.0 9 35.41126974269412 38.0 35.0 39.0 29.0 39.0 10-11 35.285467017768426 37.5 35.0 39.0 29.0 39.0 12-13 35.29286340930293 37.0 35.0 39.0 29.0 39.0 14-15 36.03825926659145 38.0 35.0 40.0 28.0 41.0 16-17 35.69407400527195 38.0 34.0 40.0 27.0 41.0 18-19 35.97112495389756 38.5 35.0 40.0 27.0 41.0 20-21 35.98480348154818 39.0 35.0 40.0 27.0 41.0 22-23 35.80588090924869 38.5 34.5 40.0 27.0 41.0 24-25 35.630444834356624 38.0 34.0 40.0 27.0 41.0 26-27 35.715772663419614 38.0 34.0 40.0 27.0 41.0 28-29 35.47873558620614 38.0 34.0 40.0 26.5 41.0 30-31 35.31675261156792 38.0 34.0 40.0 26.5 41.0 32-33 34.98756928926301 38.0 34.0 40.0 25.0 41.0 34-35 34.727985404508274 38.0 34.0 40.0 25.0 41.0 36-37 34.45515113466253 38.0 33.0 40.0 24.0 41.0 38-39 34.25428549345888 38.0 33.0 40.0 24.0 41.0 40-41 33.87474067374656 38.0 33.0 40.0 22.0 41.0 42-43 33.61198724589417 38.0 33.0 40.0 21.0 41.0 44-45 33.22210734276354 37.0 31.5 40.0 18.5 41.0 46-47 32.81083115928666 37.0 31.0 40.0 15.5 41.0 48-49 32.40482726498601 37.0 31.0 40.0 12.5 41.0 50-51 31.73383973705335 36.0 30.0 38.5 9.0 40.0 52-53 31.767714186535915 36.0 30.5 39.0 9.0 40.0 54-55 31.90836257674701 36.5 31.0 39.0 8.0 40.0 56-57 31.654417427808994 36.0 30.0 39.0 7.5 40.0 58-59 31.26619822966611 36.0 29.5 39.0 6.5 40.0 60-61 30.722174801488293 35.0 28.5 39.0 2.0 40.0 62-63 30.334373237259456 35.0 28.0 38.0 2.0 40.0 64-65 29.77257121471807 34.0 28.0 38.0 2.0 40.0 66-67 29.19629031252034 34.0 26.5 37.5 2.0 39.5 68-69 28.879942453300934 34.0 26.0 37.0 2.0 39.0 70-71 28.37903531989673 34.0 26.0 36.5 2.0 39.0 72-73 27.793572912372813 33.0 26.0 36.0 2.0 39.0 74-75 27.232526834334934 32.5 25.0 35.5 2.0 38.0 76-77 26.60526713655002 32.0 23.5 35.0 2.0 37.0 78-79 25.95920238702189 31.5 21.5 35.0 2.0 36.5 80-81 25.242635473390756 31.0 19.5 34.0 2.0 36.0 82-83 24.570796745167378 31.0 18.0 34.0 2.0 35.5 84-85 23.89439082398629 30.0 12.0 34.0 2.0 35.0 86-87 23.21969340518083 29.5 7.0 34.0 2.0 35.0 88-89 22.40802833402035 29.0 2.0 34.0 2.0 35.0 90-91 21.41717797442128 29.0 2.0 33.5 2.0 34.5 92-93 20.339679086303775 26.5 2.0 33.0 2.0 34.0 94-95 19.331704041286095 25.5 2.0 32.0 2.0 34.0 96-97 18.44119328043304 25.0 2.0 32.0 2.0 34.0 98-99 17.373929813095266 22.0 2.0 32.0 2.0 34.0 100-101 15.74908845974443 10.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.846284316985436 1101 2 8.011193902961246 1101 3 3.5771530230516575 1101 4 2.2441933935077287 1101 5 1.4707200435473808 1101 6 1.2181320401247149 1101 7 0.8334375010835018 1101 8 0.8611368388106229 1101 9 0.8982338466491271 1101 10-11 0.8488326499734313 1101 12-13 0.623310873995969 1101 14-15 0.6448009966458841 1101 16-17 0.6675429582849901 1101 18-19 0.8237642701811936 1101 20-21 0.6625842002153703 1101 22-23 0.5636616672519352 1101 24-25 0.7603939607024017 1101 26-27 0.8574383047363341 1101 28-29 0.9245036976523622 1101 30-31 0.8922733428165799 1101 32-33 0.7625194010742291 1101 34-35 0.8698490034070403 1101 36-37 0.8879014783147241 1101 38-39 0.7172886554410951 1101 40-41 0.8296526516461995 1101 42-43 0.7256683299220725 1101 44-45 0.6121283514025109 1101 46-47 0.4058324968980216 1101 48-49 0.39444209789377993 1101 50-51 0.35392950765699993 1101 52-53 0.4027059109185913 1101 54-55 0.5874419691041837 1101 56-57 0.3244911561108701 1101 58-59 0.36364444575754007 1101 60-61 0.33974218978665505 1101 62-63 0.19327328063232585 1101 64-65 0.2103965217556727 1101 66-67 -0.08084337570945266 1101 68-69 -0.1204330959522828 1101 70-71 -0.45763268602815543 1101 72-73 -0.1974103261418243 1101 74-75 -0.3124027768478328 1101 76-77 -0.3981331189856938 1101 78-79 -0.5852845494411092 1101 80-81 -0.522031283857963 1101 82-83 -0.49109207573499347 1101 84-85 -0.4382157741346546 1101 86-87 -0.7881683778214743 1101 88-89 -0.5818797586991664 1101 90-91 -0.5848518651964092 1101 92-93 -0.8616016605327879 1101 94-95 -0.9073740861121387 1101 96-97 -1.497875434872558 1101 98-99 -1.4882191848594033 1101 100-101 -1.5552492573483345 1102 1 14.87463419291596 1102 2 7.969228878738157 1102 3 3.6699377920461025 1102 4 2.124670174790957 1102 5 1.5453227258113529 1102 6 1.308061734086671 1102 7 1.0233095913588173 1102 8 1.035145759996965 1102 9 0.987185754955874 1102 10-11 0.9354619480946695 1102 12-13 0.5201803351799512 1102 14-15 0.8059362323778956 1102 16-17 0.9549343974909519 1102 18-19 0.9178960792323565 1102 20-21 0.8746359253719405 1102 22-23 0.7591154070605128 1102 24-25 0.7963379226544234 1102 26-27 0.7138906561483651 1102 28-29 0.9019296990703012 1102 30-31 0.8217944937086941 1102 32-33 0.766385494658067 1102 34-35 0.6674259560243172 1102 36-37 0.7330834468837821 1102 38-39 0.5528338226394851 1102 40-41 0.6904452192936077 1102 42-43 0.2551481243212237 1102 44-45 0.19721268910780765 1102 46-47 0.11310902413422497 1102 48-49 -0.12223195386091845 1102 50-51 -0.1270125341721311 1102 52-53 -0.013437153006734093 1102 54-55 0.21701112306849524 1102 56-57 -0.020016553921035296 1102 58-59 -0.16178167382180675 1102 60-61 0.13674975211679552 1102 62-63 -0.14509225168481876 1102 64-65 -0.2169799995724624 1102 66-67 -0.22259349387086047 1102 68-69 -0.6031955619808791 1102 70-71 -0.3064134079943628 1102 72-73 -0.43866195086118864 1102 74-75 -0.4989094825077629 1102 76-77 -0.7127349150391034 1102 78-79 -0.6929591329221445 1102 80-81 -0.4491220022793243 1102 82-83 -0.6195342652658944 1102 84-85 -0.8280679246722897 1102 86-87 -1.0418875081535113 1102 88-89 -0.7824676117063731 1102 90-91 -0.5982284751455964 1102 92-93 -0.29036879393701653 1102 94-95 -0.2509150881208875 1102 96-97 -0.8130392361250678 1102 98-99 -1.3648645824725438 1102 100-101 -1.3426709754152135 1103 1 14.754546260835909 1103 2 7.750942182616409 1103 3 3.4207257876567176 1103 4 1.8088532294561261 1103 5 1.3414376150660274 1103 6 0.8604823930719121 1103 7 0.6902531213481353 1103 8 0.6668514835234873 1103 9 0.8818000955374714 1103 10-11 0.7854776502518206 1103 12-13 0.4551555366007207 1103 14-15 0.6180572147914845 1103 16-17 0.7449851858475327 1103 18-19 0.6740098133858083 1103 20-21 0.6442320370124719 1103 22-23 0.6985375676773344 1103 24-25 0.8074570352479142 1103 26-27 0.7630591637575961 1103 28-29 0.8567112766497829 1103 30-31 0.6436782472100901 1103 32-33 0.6502572134486755 1103 34-35 0.8017986999672502 1103 36-37 0.7192164567887787 1103 38-39 0.6855179529926616 1103 40-41 0.7103768517403921 1103 42-43 0.6578283201108803 1103 44-45 0.40599488755420055 1103 46-47 0.17467465554143047 1103 48-49 0.25053617400878636 1103 50-51 0.32962460521970627 1103 52-53 0.4494503473542366 1103 54-55 0.14279207978108133 1103 56-57 0.19062571845183385 1103 58-59 0.1273739110925689 1103 60-61 0.33532320211535094 1103 62-63 0.17666516262110932 1103 64-65 0.03524826286653493 1103 66-67 0.14291355946593498 1103 68-69 0.004874444372180875 1103 70-71 0.17725111451567344 1103 72-73 0.30177633785636004 1103 74-75 -0.1979595922423769 1103 76-77 -0.17405923858295225 1103 78-79 -0.20075606612841668 1103 80-81 -0.04305970622688449 1103 82-83 -0.22220778312664535 1103 84-85 -0.21449025133491162 1103 86-87 -0.3148617493963961 1103 88-89 -0.5161272611331569 1103 90-91 -0.5698203851054586 1103 92-93 -0.3268672850860561 1103 94-95 -0.34846591257437964 1103 96-97 -0.9087446059018482 1103 98-99 -0.7789459882307241 1103 100-101 -0.7541187504014975 1104 1 14.968795869562666 1104 2 7.97736973987303 1104 3 3.616576657172992 1104 4 2.1099353587088387 1104 5 1.5053562302310866 1104 6 1.179269318917008 1104 7 0.9044936518093465 1104 8 0.9568345253234583 1104 9 1.0288738074223716 1104 10-11 0.7493359857336799 1104 12-13 0.7284619861637012 1104 14-15 0.7338553940545438 1104 16-17 0.8076664334190937 1104 18-19 0.7930422155311874 1104 20-21 0.9569160080637005 1104 22-23 0.8745987261366537 1104 24-25 0.9463254476045222 1104 26-27 0.867624260368494 1104 28-29 0.9380770472311184 1104 30-31 0.9229315053996032 1104 32-33 0.8362047846495102 1104 34-35 0.9794465576781519 1104 36-37 1.0925644848079301 1104 38-39 0.8336810567427051 1104 40-41 1.0618702264222009 1104 42-43 1.0807362338165163 1104 44-45 0.7039795126036736 1104 46-47 0.5687245787392996 1104 48-49 0.8451226227002451 1104 50-51 0.79932407831717 1104 52-53 0.803648318906582 1104 54-55 0.5045449839182297 1104 56-57 0.5291333605357629 1104 58-59 0.5457550384678989 1104 60-61 0.4080357515465103 1104 62-63 0.2814439616028892 1104 64-65 0.4807067697460461 1104 66-67 0.4113974528072113 1104 68-69 0.4457048514779167 1104 70-71 0.39219829716674326 1104 72-73 0.16356573662099194 1104 74-75 0.14322232530518164 1104 76-77 0.10940651638376409 1104 78-79 0.24485096117537708 1104 80-81 -0.12988369318323123 1104 82-83 -0.05659407421941509 1104 84-85 0.07209425097641287 1104 86-87 0.21915469315815272 1104 88-89 0.1442361236799954 1104 90-91 0.017812656876074584 1104 92-93 0.2233484145032456 1104 94-95 -0.05100419562016967 1104 96-97 -0.5020807958627387 1104 98-99 -0.7192629286373595 1104 100-101 -0.931115514907658 1105 1 14.98584108946768 1105 2 8.148128714534543 1105 3 3.8218877099442956 1105 4 2.3199903718352957 1105 5 1.5529787192432138 1105 6 1.1909024565825632 1105 7 1.0160641069581615 1105 8 1.019895349487932 1105 9 0.9073006974846081 1105 10-11 0.7577218003114154 1105 12-13 0.7494598918301918 1105 14-15 0.800086478683312 1105 16-17 1.0512706043875042 1105 18-19 1.0546366375246308 1105 20-21 0.8796133473752192 1105 22-23 0.8964306779964772 1105 24-25 1.0569803692882473 1105 26-27 1.160575795721435 1105 28-29 1.3501131966072464 1105 30-31 1.1327756102932582 1105 32-33 0.7997906670718606 1105 34-35 0.8921899133313786 1105 36-37 1.0061540544371468 1105 38-39 1.047343264347198 1105 40-41 1.128909915516921 1105 42-43 1.0929560060946244 1105 44-45 1.0794684362342437 1105 46-47 1.0442769223279882 1105 48-49 0.6898985880110047 1105 50-51 0.8989726875027415 1105 52-53 0.9948038157945831 1105 54-55 1.1254573463854172 1105 56-57 1.1493492096257647 1105 58-59 1.0773090486740884 1105 60-61 1.213145482822874 1105 62-63 1.1162172721247074 1105 64-65 1.1551053863060297 1105 66-67 0.9620637310880902 1105 68-69 0.837762976606971 1105 70-71 0.9592988251747698 1105 72-73 0.9483659637529342 1105 74-75 0.7168643270160473 1105 76-77 0.5578726383270727 1105 78-79 0.5635063843192363 1105 80-81 0.5809990978848774 1105 82-83 0.6318306772628439 1105 84-85 0.6188019439648187 1105 86-87 0.622808715163405 1105 88-89 0.7639814851737583 1105 90-91 0.797902477235354 1105 92-93 1.2139928645194686 1105 94-95 1.041736800220658 1105 96-97 0.7581943435136829 1105 98-99 0.2925040546374724 1105 100-101 0.2630966764731273 1106 1 14.852886225436283 1106 2 8.022400503861 1106 3 3.5564192983446823 1106 4 2.0866352422438297 1106 5 1.4690708273033621 1106 6 1.0832493513474546 1106 7 0.7404307831751353 1106 8 0.8548980605419487 1106 9 0.9917600424569954 1106 10-11 0.8960773997162192 1106 12-13 0.5629732637960458 1106 14-15 0.7001279146793351 1106 16-17 1.0372397445828838 1106 18-19 0.817709578740974 1106 20-21 0.6873955742596323 1106 22-23 0.7493548424219014 1106 24-25 0.8205600927607435 1106 26-27 0.7756507964146024 1106 28-29 0.9008447575890131 1106 30-31 0.5749404331351258 1106 32-33 0.5518736345669382 1106 34-35 0.7108610270508535 1106 36-37 1.0584743161770902 1106 38-39 0.9592569218979534 1106 40-41 0.8435196854778297 1106 42-43 0.7055423439227582 1106 44-45 0.7965152666116069 1106 46-47 0.6596453638108102 1106 48-49 0.7846506960095354 1106 50-51 0.7947544183874982 1106 52-53 0.7476417579197374 1106 54-55 0.6003885687935053 1106 56-57 0.5927107086537937 1106 58-59 0.5969732399635959 1106 60-61 0.6787670843780305 1106 62-63 0.5529411173851351 1106 64-65 0.5541950652001653 1106 66-67 0.5203993364695272 1106 68-69 0.5340939269854381 1106 70-71 0.5697407731903077 1106 72-73 0.4478820945243207 1106 74-75 0.27161394731444943 1106 76-77 0.3201963813068254 1106 78-79 0.1268389551686262 1106 80-81 0.3874862573472271 1106 82-83 0.41720242284274534 1106 84-85 0.18452979265103764 1106 86-87 0.3562162173892425 1106 88-89 0.35929063005186634 1106 90-91 0.7049537592866351 1106 92-93 0.7583195603915804 1106 94-95 0.4507075778310501 1106 96-97 0.17519392299223213 1106 98-99 0.12572551848341007 1106 100-101 0.09948706979161237 1107 1 14.808767319850944 1107 2 8.115897019678744 1107 3 3.619327811211665 1107 4 2.1677374016817197 1107 5 1.3178699025767955 1107 6 1.1479740147884243 1107 7 0.7816705673281561 1107 8 0.8490352631666553 1107 9 0.6680054512554463 1107 10-11 0.5923366716683063 1107 12-13 0.46098363018216304 1107 14-15 0.5751699785079296 1107 16-17 0.8635450786055046 1107 18-19 0.6177173235195426 1107 20-21 0.6564627350431707 1107 22-23 0.4899645888384896 1107 24-25 0.719473887566707 1107 26-27 0.6521434572688136 1107 28-29 0.8247652806713575 1107 30-31 0.7381054917921404 1107 32-33 0.5940253663962309 1107 34-35 0.4041031268926645 1107 36-37 0.22606590302412854 1107 38-39 0.26636120727754786 1107 40-41 0.6271749111703571 1107 42-43 0.4368873989784561 1107 44-45 0.4580200383633013 1107 46-47 0.3887788973466684 1107 48-49 0.7564887449449245 1107 50-51 0.747373799308626 1107 52-53 0.7483045173456162 1107 54-55 0.5863176614733376 1107 56-57 0.4069322519170946 1107 58-59 0.5272653923730921 1107 60-61 0.6428237606675857 1107 62-63 0.3614406246235973 1107 64-65 0.24239453971693692 1107 66-67 0.13172336445141397 1107 68-69 0.4460700653226759 1107 70-71 0.5757414276240951 1107 72-73 0.31392724271097805 1107 74-75 0.2804036901297131 1107 76-77 0.17989371536042498 1107 78-79 0.42061892988192184 1107 80-81 0.28978316505076407 1107 82-83 0.19499562563783712 1107 84-85 0.4224029215609377 1107 86-87 0.5301639440063006 1107 88-89 0.3538290122063472 1107 90-91 0.6968962852848897 1107 92-93 0.7631298423596071 1107 94-95 0.6819225206068147 1107 96-97 -0.18381083423799893 1107 98-99 -0.17910238285585578 1107 100-101 -0.007107802777737149 1108 1 14.58024138002872 1108 2 7.661377931667879 1108 3 3.2886822600935446 1108 4 -0.4152351181812506 1108 5 0.3158860998598598 1108 6 0.6232104329714261 1108 7 0.4513211863313771 1108 8 0.4623164981807122 1108 9 0.7318485271279442 1108 10-11 0.7564158779937671 1108 12-13 0.40814833836635245 1108 14-15 0.5575626264299416 1108 16-17 0.8706794408614584 1108 18-19 0.6062856172036746 1108 20-21 0.4604420414986876 1108 22-23 -0.014826883610396635 1108 24-25 0.5049887900645871 1108 26-27 0.4317666076442066 1108 28-29 0.3695356912813068 1108 30-31 0.445358398824645 1108 32-33 0.6996867426665574 1108 34-35 0.5212618119280137 1108 36-37 0.2784985646037299 1108 38-39 0.28864436432827745 1108 40-41 0.356087368346536 1108 42-43 0.4164443714446122 1108 44-45 0.3276860029686688 1108 46-47 0.21291983135674286 1108 48-49 0.4532836241174891 1108 50-51 0.4561238616501342 1108 52-53 0.654106881335629 1108 54-55 0.7055478109778619 1108 56-57 0.771266195238109 1108 58-59 0.625931859546931 1108 60-61 0.6581960257935542 1108 62-63 0.6914518269131626 1108 64-65 0.4771981735555251 1108 66-67 0.6021575682640297 1108 68-69 0.5497042875500107 1108 70-71 0.34364744348377485 1108 72-73 0.362920187570392 1108 74-75 0.42846151235858443 1108 76-77 0.4740326544422224 1108 78-79 0.687860481168844 1108 80-81 0.6432228817709493 1108 82-83 0.5956620084108657 1108 84-85 0.36213498734753813 1108 86-87 0.39299919745810286 1108 88-89 0.804291106650858 1108 90-91 1.3250090447694305 1108 92-93 1.7446318698972867 1108 94-95 1.4663202632687415 1108 96-97 0.6519446105734623 1108 98-99 0.44815984662995234 1108 100-101 0.5709376278774041 1109 1 14.429496606924712 1109 2 6.639035900984204 1109 3 2.9391585103521862 1109 4 -3.7538900860863755 1109 5 -0.6284901483063976 1109 6 0.019459482679536677 1109 7 0.001218775670444927 1109 8 0.09442276348128331 1109 9 0.43015904183160103 1109 10-11 0.35949203386255846 1109 12-13 0.2643491237084916 1109 14-15 0.41057194219653326 1109 16-17 0.5309858358899433 1109 18-19 0.459673306070556 1109 20-21 0.5354255930213654 1109 22-23 0.41070568669957197 1109 24-25 0.6732805430421678 1109 26-27 0.6540122723370487 1109 28-29 0.6857102985521166 1109 30-31 0.5214695138105441 1109 32-33 0.6187904575813263 1109 34-35 0.61204926970251 1109 36-37 0.653221032875031 1109 38-39 0.6862738074514212 1109 40-41 0.8031819744084032 1109 42-43 0.7149232069621547 1109 44-45 0.5432319645478216 1109 46-47 0.4113651887013603 1109 48-49 0.16426295367057975 1109 50-51 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58-59 0.24646237379724312 2312 60-61 0.28511284735489184 2312 62-63 0.2867976292675607 2312 64-65 0.3083920242370617 2312 66-67 0.2568559108910442 2312 68-69 0.6529353904000743 2312 70-71 0.6807443301008789 2312 72-73 0.22900173961500414 2312 74-75 0.46476611273033086 2312 76-77 0.6802710373418499 2312 78-79 0.7574426527921396 2312 80-81 0.6546428786730552 2312 82-83 0.4716413299722042 2312 84-85 0.5995362419943788 2312 86-87 0.6506273727988692 2312 88-89 1.057071078769816 2312 90-91 1.4757149772353557 2312 92-93 1.848544896539174 2312 94-95 1.8920908642600658 2312 96-97 1.6134637400654057 2312 98-99 1.1112217270512659 2312 100-101 1.1169421198228822 2313 1 -14.350365807084042 2313 2 -7.504851892810969 2313 3 -3.4624235702203237 2313 4 -0.8949952863302002 2313 5 -0.4207788413978051 2313 6 -0.7582884768837985 2313 7 -0.22430192160079798 2313 8 0.0884384061693595 2313 9 -2.6032000196071152 2313 10-11 -0.456502337619618 2313 12-13 -0.6669693031563995 2313 14-15 -0.653715666356284 2313 16-17 -0.5652396531419654 2313 18-19 0.11121283631493384 2313 20-21 -0.9164543398480731 2313 22-23 -1.8199533090335223 2313 24-25 -1.0857559592021175 2313 26-27 -0.1909503625375919 2313 28-29 0.40804407520049324 2313 30-31 -0.8975168384190937 2313 32-33 -0.06780302252096249 2313 34-35 -0.8523562923180634 2313 36-37 -0.987275202905245 2313 38-39 -0.22935047513025353 2313 40-41 0.0434816870452579 2313 42-43 0.3968072563284579 2313 44-45 0.1304443045387913 2313 46-47 -0.43734682276149073 2313 48-49 0.3798702161210059 2313 50-51 -0.4828217565952784 2313 52-53 0.49088397417831686 2313 54-55 -0.46348541098815943 2313 56-57 0.08270722546413722 2313 58-59 0.4045873737972414 2313 60-61 -0.9550374691008052 2313 62-63 -0.0817031618716797 2313 64-65 0.5031902837307314 2313 66-67 -0.697815766324144 2313 68-69 0.09341007394437639 2313 70-71 0.5684698047844208 2313 72-73 -0.24201487430904578 2313 74-75 0.5302684861480529 2313 76-77 0.8924941386076704 2313 78-79 0.6829450262098611 2313 80-81 0.6845993660148295 2313 82-83 0.8245171211114446 2313 84-85 1.092633552120958 2313 86-87 1.0472927209001348 2313 88-89 1.5396541483900705 2313 90-91 1.758661970906239 2313 92-93 2.414743472488542 2313 94-95 2.3136494085638653 2313 96-97 2.007534151457808 2313 98-99 1.7933934042664568 2313 100-101 1.5291493983038915 2314 1 -14.350365807084042 2314 2 -7.382172427456757 2314 3 -3.2347313792176102 2314 4 -1.0451082159261773 2314 5 -1.065492219341138 2314 6 -5.7091708446971765 2314 7 -1.4525376916289403 2314 8 -3.735879381084768 2314 9 -1.3720558911033507 2314 10-11 -0.2671215120537056 2314 12-13 -2.6593267506104254 2314 14-15 -2.4815970565701733 2314 16-17 -2.183605882136213 2314 18-19 -1.018725726613816 2314 20-21 -1.6562177776272335 2314 22-23 -0.6441088653381897 2314 24-25 -2.9434562406833464 2314 26-27 -4.726864923713407 2314 28-29 -1.975622849912014 2314 30-31 -1.7075005731862376 2314 32-33 -3.8215983839799392 2314 34-35 -2.349468581417824 2314 36-37 -0.6572405655440932 2314 38-39 -1.0868034070859593 2314 40-41 -2.027610864657955 2314 42-43 -1.1879063744423277 2314 44-45 -2.1372409758468507 2314 46-47 -1.8096419903860976 2314 48-49 -0.6593943088162142 2314 50-51 -1.8020577120302264 2314 52-53 -0.5529293380298768 2314 54-55 0.1145687446524164 2314 56-57 -0.5924330937726587 2314 58-59 -0.9597601628206007 2314 60-61 -0.12640096755855623 2314 62-63 -2.2886238920373643 2314 64-65 -0.49764783927825107 2314 66-67 -1.7762453707334558 2314 68-69 -1.6131731315706297 2314 70-71 -1.9484030567166997 2314 72-73 -1.9887150546592984 2314 74-75 -1.6060265803455671 2314 76-77 -0.14856967504163876 2314 78-79 0.28749549899719895 2314 80-81 0.48388597121633126 2314 82-83 0.3147267993463707 2314 84-85 0.4851283691581614 2314 86-87 0.5788451657416154 2314 88-89 0.7281151034036384 2314 90-91 1.3373498806974062 2314 92-93 1.8838374231703305 2314 94-95 1.9525810440203877 2314 96-97 1.891255434472864 2314 98-99 1.8291132883029313 2314 100-101 1.5868214473861304 2315 1 -14.350365807084042 2315 2 -7.5143386466326465 2315 3 -4.536375634964045 2315 4 -2.6208996925002204 2315 5 -3.460463769114483 2315 6 -2.6848344407767186 2315 7 -2.9920787771033197 2315 8 -3.614610289964002 2315 9 -2.2039539796264833 2315 10-11 -4.493114359477541 2315 12-13 -4.078087619542924 2315 14-15 -3.1295145872942314 2315 16-17 -4.505482269175715 2315 18-19 -1.3956480269347011 2315 20-21 -3.004389250272091 2315 22-23 -4.2540578950472145 2315 24-25 -4.982384754941208 2315 26-27 -4.584143106537038 2315 28-29 -2.1621364601802853 2315 30-31 -3.1280591362678862 2315 32-33 -4.590976917519921 2315 34-35 -3.92362107754321 2315 36-37 -4.842872837269223 2315 38-39 -3.220853726150523 2315 40-41 -4.423596544953078 2315 42-43 -3.3867137023766674 2315 44-45 -2.054431534155267 2315 46-47 -1.2422432397221357 2315 48-49 -1.0787795729085374 2315 50-51 -0.9218115193399683 2315 52-53 -0.7010005800168813 2315 54-55 -2.0345452781805236 2315 56-57 -4.165279044163032 2315 58-59 -3.700262352978715 2315 60-61 -4.385225267399207 2315 62-63 -2.607924023737901 2315 64-65 -2.9536896014459977 2315 66-67 -1.6340542667045845 2315 68-69 -1.22052089375293 2315 70-71 -1.9222457655275385 2315 72-73 -2.166764995357674 2315 74-75 -2.825631769783328 2315 76-77 -2.5511326511827406 2315 78-79 -1.8218623335466582 2315 80-81 -1.3190234464635537 2315 82-83 -1.0638795033611324 2315 84-85 -0.5882796733061681 2315 86-87 -1.5067001545235321 2315 88-89 -2.00619907150341 2315 90-91 -1.6204718397045355 2315 92-93 -0.6803229313296804 2315 94-95 -0.4258565401115142 2315 96-97 -1.366664333705085 2315 98-99 -0.7801358549159474 2315 100-101 -0.4638946288109995 2316 1 -14.350365807084042 2316 2 -7.532195578733816 2316 3 -5.177355995879434 2316 4 -3.0744427533176477 2316 5 -1.9158376670715 2316 6 -1.0628705913272967 2316 7 -4.554226920005988 2316 8 -3.0955893526225964 2316 9 -1.1572964007573816 2316 10-11 -4.498600969729047 2316 12-13 -3.84348892842182 2316 14-15 -2.1026760786372236 2316 16-17 -3.3640585830706087 2316 18-19 -2.66333013695607 2316 20-21 -3.35706660985381 2316 22-23 -3.2834383314975426 2316 24-25 -3.4975470665464385 2316 26-27 -4.264012897281646 2316 28-29 -3.626542094614777 2316 30-31 -2.687452909356203 2316 32-33 -3.0694258547196966 2316 34-35 -4.271368011886821 2316 36-37 -2.5388387993365207 2316 38-39 -3.8120452013243096 2316 40-41 -3.185263152608073 2316 42-43 -7.038188551601845 2316 44-45 -3.8142363235426053 2316 46-47 -5.244091042717567 2316 48-49 -4.789812690259147 2316 50-51 -3.738941722694193 2316 52-53 -4.787985260586698 2316 54-55 -4.8115468065828395 2316 56-57 -4.971824766406893 2316 58-59 -3.4252362403136303 2316 60-61 -3.230287170643667 2316 62-63 -2.2676405906748442 2316 64-65 -2.3145437658997245 2316 66-67 -2.583566830289662 2316 68-69 -2.4081175253666665 2316 70-71 -2.6712204377177002 2316 72-73 -3.55802197348795 2316 74-75 -3.898024339033526 2316 76-77 -3.7180213492672216 2316 78-79 -3.8464097140185167 2316 80-81 -3.775670521254952 2316 82-83 -3.4085519295670323 2316 84-85 -3.3076710564217464 2316 86-87 -3.334315256790763 2316 88-89 -3.0400835738579737 2316 90-91 -2.5605388474586697 2316 92-93 -2.707996455152692 2316 94-95 -1.7140038981589392 2316 96-97 -1.9253521796610151 2316 98-99 -2.274725222192794 2316 100-101 -2.1016625418041883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8225.0 3 2234.0 4 2265.0 5 2779.0 6 3377.0 7 3991.0 8 5086.0 9 6187.0 10 7704.0 11 9727.0 12 11858.0 13 14028.0 14 15228.0 15 18085.0 16 21031.0 17 22356.0 18 19077.0 19 18026.0 20 19262.0 21 21047.0 22 24025.0 23 26619.0 24 30432.0 25 34485.0 26 39646.0 27 45383.0 28 52032.0 29 60372.0 30 70160.0 31 81056.0 32 94842.0 33 111645.0 34 131157.0 35 156308.0 36 167847.0 37 103627.0 38 13698.0 39 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83875045116685 19.577693448122645 14.199496993898642 22.384059106811865 2 15.09038503032285 21.419101292843155 41.03837275733713 22.45214091949687 3 18.30092685601968 27.11956551248568 30.832758686220956 23.746748945273684 4 12.023623544754324 17.332536372651568 38.94577044815454 31.69806963443957 5 12.615182663278382 38.57567168393389 34.77287906431224 14.03626658847549 6 29.423673580230155 37.735293119250485 18.689364880002714 14.15166842051665 7 26.368995928199983 34.50112132361678 22.293257867767327 16.83662488041591 8 25.59673179183844 34.61254868093011 21.025137555877834 18.765581971353615 9 27.173882789179515 16.224276503865187 21.070982694747734 35.53085801220757 10-11 24.016671078800712 27.19656396576184 28.396291092240755 20.390473863196693 12-13 25.65180481648721 25.172898714193632 28.593060155344936 20.58223631397422 14-15 22.844203685699853 25.691645801311125 27.712914885643368 23.751235627345654 16-17 21.103490319527882 29.039990712938142 28.110266208740292 21.746252758793684 18-19 20.968811241083603 27.38192914271903 31.155152168976088 20.494107447221282 20-21 22.170031395990847 26.628492999230552 30.872724333964243 20.32875127081436 22-23 21.7880984532119 26.71744966078255 30.57806891942193 20.916382966583615 24-25 21.37769940220698 26.812530383084194 30.961649456238337 20.84812075847049 26-27 21.28138026990157 26.890391210535185 30.980254683602094 20.84797383596115 28-29 21.094291105551623 26.916062454248852 31.140165392003503 20.849481048196026 30-31 21.232399222960048 27.056393830699598 30.953654146557234 20.757552799783113 32-33 20.93870774987949 27.16206442872677 30.825352297359593 21.07387552403415 34-35 21.363551140300586 27.08838857987539 30.608810190833573 20.939250088990455 36-37 21.49481959249544 26.90633154879717 30.65266885737612 20.94618000133127 38-39 21.29890867302029 27.04426842785886 30.803804294671867 20.853018604448984 40-41 21.38374241556216 26.93422479378921 30.832745550366912 20.849287240281715 42-43 21.112067958501196 27.211449388357188 30.424068273626965 21.252414379514654 44-45 21.341341851299006 27.378908525267757 30.07585676368249 21.203892859750745 46-47 21.39043591637287 27.334713031969283 29.653544355657864 21.621306695999984 48-49 21.546556468026687 27.506842967277755 29.384450590497877 21.562149974197673 50-51 21.604510421890524 27.76410797940221 29.199470785137947 21.431910813569317 52-53 21.502177647800515 27.926128205084634 29.147854044069543 21.423840103045308 54-55 21.38873106575471 27.83833579809271 29.116839109206964 21.65609402694562 56-57 21.436026080410066 28.21795868336871 28.830390363620058 21.515624872601165 58-59 21.371778902772476 27.786569157064445 29.07239327303277 21.76925866713031 60-61 21.51537792751182 27.797268108460578 29.15183665706678 21.535517306960823 62-63 21.6156798867529 27.62007880835143 29.143534855476133 21.620706449419533 64-65 21.503440280126178 27.601434862207107 29.202495489312398 21.692629368354314 66-67 21.29939316052378 27.88503468915609 28.890694177085187 21.924877973234942 68-69 21.322337118913488 27.907772794556863 29.0107787668379 21.75911131969175 70-71 21.299550464411425 27.83252252093146 28.90310735192428 21.964819662732836 72-73 21.44743193560145 27.683841900568417 28.65588714967391 22.212839014156227 74-75 21.341196338979135 28.027911291432417 28.485997631583864 22.144894738004584 76-77 21.475055998956137 27.77938993758563 28.714959876476087 22.030594186982142 78-79 21.23649584193403 27.86221893767573 28.67772652752648 22.223558692863758 80-81 21.197045528451415 28.092522789370726 28.488047112247695 22.222384569930163 82-83 21.35270178166258 27.976125354460407 28.38703780843067 22.284135055446338 84-85 21.26225737871356 27.73380008376236 28.290075926200807 22.713866611323272 86-87 21.33754975836746 27.630900430271023 28.55085697513967 22.48069283622185 88-89 21.493754902470354 27.43052972328071 28.42833212565679 22.64738324859215 90-91 21.095454706024615 27.68187833383604 28.660460262984127 22.56220669715522 92-93 21.027928975994158 27.70296273022936 28.6203306641305 22.648777629645984 94-95 21.13209111531859 27.56718864470109 28.381757091964822 22.9189631480155 96-97 21.28003725128986 27.433161805192068 28.127714823702156 23.159086119815917 98-99 21.15844913258072 27.294888561106884 28.08457954139453 23.46208276491787 100-101 21.29848789393944 27.30102172452088 28.306429654587973 23.09406072695171 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3132.0 1 2239.0 2 1207.5 3 1002.0 4 918.0 5 938.0 6 1320.0 7 3031.5 8 4170.0 9 3549.5 10 2886.0 11 2640.5 12 2650.5 13 2653.0 14 2795.0 15 2955.0 16 3039.5 17 3217.5 18 3360.0 19 3533.0 20 3973.0 21 4556.0 22 5262.5 23 6141.5 24 7163.0 25 8623.0 26 10649.0 27 12895.0 28 14943.0 29 17397.5 30 20157.0 31 23395.0 32 27258.5 33 30790.0 34 34185.0 35 37328.5 36 41079.5 37 44410.5 38 45526.0 39 45722.5 40 46001.5 41 46724.5 42 47674.5 43 49391.0 44 51354.5 45 52089.5 46 52601.5 47 55404.0 48 58072.5 49 55843.0 50 51777.0 51 48861.5 52 46483.5 53 44725.5 54 42609.5 55 39016.5 56 34841.5 57 31323.5 58 28142.0 59 24399.5 60 19986.5 61 15670.5 62 11928.0 63 8765.0 64 6306.5 65 4511.0 66 3189.5 67 2213.5 68 1427.5 69 880.0 70 544.0 71 344.0 72 215.5 73 152.5 74 110.0 75 74.5 76 55.5 77 34.0 78 18.5 79 11.0 80 10.0 81 12.0 82 10.0 83 8.0 84 7.0 85 3.5 86 2.5 87 2.0 88 1.5 89 2.0 90 2.0 91 1.5 92 1.0 93 2.0 94 3.0 95 3.0 96 1.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 52.85218200160545 2 0.07932332458290847 3 0.1090187230165101 4 0.18413858937365762 5 0.1128153949623587 6 0.13735816718373722 7 0.14664645390840256 8 0.1437311522356974 9 0.13254452953810775 10-11 0.11973076172086869 12-13 0.13095128327511768 14-15 0.13830733517019939 16-17 0.13281572039138262 18-19 0.13949379515327706 20-21 0.16915529473021934 22-23 0.18739287961295642 24-25 0.21325770724405008 26-27 0.20556266678237478 28-29 0.20464739765257198 30-31 0.21946119801271344 32-33 0.21027460785802615 34-35 0.19715575033085286 36-37 0.18291823053392053 38-39 0.1789859631614345 40-41 0.17868087345150022 42-43 0.17274857353611175 44-45 0.1893929121558588 46-47 0.21403738094721544 48-49 0.21759676089644847 50-51 0.2279359121775541 52-53 0.2561736597748031 54-55 0.24190224112121145 56-57 0.2183086368862951 58-59 0.17451131408239864 60-61 0.1854945436400321 62-63 0.18963020415247434 64-65 0.17895206430477512 66-67 0.18698609333304406 68-69 0.17562997635215757 70-71 0.18915562015924325 72-73 0.20878305816501422 74-75 0.23922423144512184 76-77 0.23864795088191265 78-79 0.23312243724643655 80-81 0.2380716703187035 82-83 0.22868168702406005 84-85 0.20030834400017355 86-87 0.21105428156119152 88-89 0.21298651639077518 90-91 0.19986765886360183 92-93 0.19922358058707396 94-95 0.2387835463085501 96-97 0.26346191395656604 98-99 0.2811910159894127 100-101 0.2766485691970581 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1474976.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.35231528119182 #Duplication Level Percentage of deduplicated Percentage of total 1 84.1879481322262 61.75380914271681 2 9.949655271068105 14.596605007651196 3 2.8910500359472255 6.361956411915055 4 1.1723813154636098 3.4398753552666044 5 0.576735859058797 2.1152455283824945 6 0.33075363499484456 1.4556926948725246 7 0.21440266061050883 1.1008852090759886 8 0.14189867349843688 0.8326876989152194 9 0.09879024885261956 0.6521844132490274 >10 0.41252366779029315 5.068807959827839 >50 0.016872099375679475 0.8348758724922628 >100 0.006049695995410479 0.7189560986106941 >500 6.258034121961086E-4 0.3168915052126216 >1k 3.129017060980543E-4 0.7515271018115842 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4523 0.306649057340594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2962 0.200816826850064 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2338 0.1585110537391795 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.3559542663745038E-4 0.0 0.0 0.0 0.0 3 1.3559542663745038E-4 0.0 0.0 0.0 0.0 4 1.3559542663745038E-4 0.0 0.0 0.0 0.0 5 1.3559542663745038E-4 0.0 0.0 0.0 0.0 6 1.3559542663745038E-4 0.0 0.0 0.0 0.0 7 1.3559542663745038E-4 0.0 0.0 0.0 0.0 8 1.3559542663745038E-4 0.0 0.0 0.0 0.0 9 1.3559542663745038E-4 0.0 0.0 0.0 0.0 10-11 1.3559542663745038E-4 0.0 0.0 3.3898856659362594E-5 0.0 12-13 1.6949428329681297E-4 0.0 0.0 2.0339313995617556E-4 0.0 14-15 2.0339313995617556E-4 0.0 0.0 2.0339313995617556E-4 0.0 16-17 2.0339313995617556E-4 0.0 0.0 4.406851365717137E-4 0.0 18-19 2.7119085327490075E-4 0.0 0.0 6.440782765278893E-4 0.0 20-21 3.3898856659362594E-4 0.0 0.0 0.001084763413099603 0.0 22-23 3.3898856659362594E-4 0.0 0.0 0.0015254485496713167 0.0 24-25 4.0678627991235113E-4 0.0 0.0 0.0018644371162649426 0.0 26-27 4.0678627991235113E-4 0.0 0.0 0.002644110819430282 0.0 28-29 4.0678627991235113E-4 0.0 0.0 0.0049831319289263005 0.0 30-31 4.745839932310763E-4 0.0 0.0 0.012847666673898424 0.0 32-33 4.745839932310763E-4 0.0 0.0 0.030373375566788884 0.0 34-35 5.084828498904389E-4 0.0 0.0 0.052814418675286925 0.0 36-37 5.423817065498015E-4 0.0 0.0 0.09135741869698219 0.0 38-39 6.101794198685266E-4 0.0 0.0 0.14481591564879698 0.0 40-41 6.101794198685266E-4 0.0 0.0 0.21102038270453213 0.0 42-43 6.101794198685266E-4 0.0 0.0 0.27746214175688283 0.0 44-45 6.779771331872519E-4 0.0 0.0 0.3572939491896817 0.0 46-47 7.118759898466144E-4 0.0 0.0 0.4495666370164667 0.0 48-49 7.45774846505977E-4 0.0 0.0 0.5562124400668214 0.0 50-51 7.45774846505977E-4 0.0 0.0 0.6704515870088734 0.0 52-53 7.45774846505977E-4 0.0 0.0 0.7817415334215607 0.0 54-55 8.135725598247023E-4 0.0 0.0 0.9024553619855509 0.0 56-57 8.813702731434274E-4 0.0 0.0 1.0447966610982145 0.0 58-59 8.813702731434274E-4 0.0 0.0 1.2068331959299676 0.0 60-61 8.813702731434274E-4 0.0 0.0 1.3916158635801532 0.0 62-63 9.830668431215152E-4 0.0 0.0 1.5825342242856832 0.0 64-65 0.0010169656997808779 0.0 0.0 1.7730118998546418 0.0 66-67 0.001084763413099603 0.0 0.0 1.9878628533616818 0.0 68-69 0.0011525611264183281 0.0 0.0 2.235900787538238 0.0 70-71 0.0011525611264183281 0.0 0.0 2.518990139500575 0.0 72-73 0.0011525611264183281 0.0 0.0 2.8163170112598443 0.0 74-75 0.0011525611264183281 0.0 0.0 3.111474356192914 0.0 76-77 0.0011525611264183281 6.779771331872519E-5 0.0 3.4311405744907035 0.0 78-79 0.0011525611264183281 6.779771331872519E-5 0.0 3.7911803310697936 0.0 80-81 0.0013559542663745038 6.779771331872519E-5 0.0 4.1829155186253875 0.0 82-83 0.001423751979693229 6.779771331872519E-5 0.0 4.6024477686416585 0.0 84-85 0.001423751979693229 6.779771331872519E-5 0.0 5.033064944785542 0.0 86-87 0.001423751979693229 6.779771331872519E-5 0.0 5.4876147137309355 0.0 88-89 0.001423751979693229 6.779771331872519E-5 0.0 5.962571594385265 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5565 0.0 44.833046 1 GTATCAA 8395 0.0 38.07449 1 GTACATG 6070 0.0 33.50977 1 TATCAAC 11200 0.0 27.458763 2 TACATGG 7145 0.0 26.645311 2 ATCAACG 12060 0.0 25.579887 3 ACATGGG 7065 0.0 25.209368 3 TCAACGC 12295 0.0 25.204527 4 CAACGCA 12565 0.0 24.71712 5 GTGTACG 205 0.0013994883 24.43882 1 AACGCAG 12850 0.0 24.168919 6 ACGCAGA 13240 0.0 23.136152 7 GAGTACT 6610 0.0 22.955769 12-13 CATGGGA 5260 0.0 22.811363 4 CGCAGAG 13930 0.0 21.719812 8 AGTACTT 7050 0.0 21.255285 12-13 GCAGAGT 13880 0.0 20.606436 9 ACGCATA 115 0.0038633675 20.521381 7 CGGGTCG 140 5.065014E-4 20.22822 6 GTACTTT 7535 0.0 20.204119 14-15 >>END_MODULE