FastQCFastQC Report
Fri 27 May 2016
SRR939552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474976
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101460.6878755993317857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75610.5126185104028812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61450.4166169483435663No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18850.127798689605797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18750.12712071247260973No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17020.11539170806847025No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.11274759724903997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.10895092530319138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57200.036.5480041
GTATCAA91450.034.5221941
TATCAAC115500.027.1648452
ATCAACG115450.027.132983
TCAACGC116150.027.0506924
CAACGCA117700.026.6142965
AACGCAG121050.025.9381056
GTACATG72950.025.6168581
TACATGG73100.024.9136562
ACATGGG72250.024.2247563
ACGCAGA133800.023.3254857
CGCAGAG133550.023.1573458
AGTACTT74300.022.24040812-13
GAGTACT70700.022.17255812-13
CATGGGA53000.021.9859094
GCAGAGT137850.021.580739
GTACTTT78350.020.24834814-15
ATGGGAT21150.019.6288875
AGAGTAC127450.019.01370810-11
TACTTTT88400.018.58634814-15
Produced by FastQC (version 0.11.5)