##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939552_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1474976 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.381108573970018 31.0 31.0 34.0 30.0 34.0 2 31.58841703187035 31.0 31.0 34.0 30.0 34.0 3 31.6256128913284 31.0 31.0 34.0 30.0 34.0 4 35.45520808474172 37.0 35.0 37.0 33.0 37.0 5 35.20478977285054 37.0 35.0 37.0 32.0 37.0 6 35.22107817347536 37.0 35.0 37.0 32.0 37.0 7 35.12634307270084 37.0 35.0 37.0 32.0 37.0 8 35.09042452216172 37.0 35.0 37.0 32.0 37.0 9 36.66154635736446 39.0 35.0 39.0 32.0 39.0 10-11 36.536266352808454 38.0 35.0 39.0 32.0 39.0 12-13 36.467177093051006 38.0 35.0 39.0 32.0 39.0 14-15 37.47672029917774 39.0 36.0 41.0 32.0 41.0 16-17 37.43525013288352 39.0 36.0 41.0 32.0 41.0 18-19 37.44426383886925 39.0 36.0 41.0 32.0 41.0 20-21 37.403140457878635 39.0 36.0 41.0 32.0 41.0 22-23 37.25796690929208 39.0 36.0 40.5 31.0 41.0 24-25 37.119390078211445 39.0 36.0 40.0 31.0 41.0 26-27 36.80589107890569 39.0 35.5 40.0 30.5 41.0 28-29 36.58844957477274 38.5 35.0 40.0 30.0 41.0 30-31 36.30781687295251 38.0 35.0 40.0 30.0 41.0 32-33 36.0813640357538 38.0 35.0 40.0 30.0 41.0 34-35 36.014144297941115 38.0 35.0 40.0 30.0 41.0 36-37 35.883411323302894 38.0 35.0 40.0 29.5 41.0 38-39 35.895038631137055 38.0 35.0 40.0 29.0 41.0 40-41 35.75380480767144 38.0 35.0 40.0 29.0 41.0 42-43 35.53930674126223 38.0 34.0 40.0 27.5 41.0 44-45 35.26423311294557 38.0 34.0 40.0 27.0 41.0 46-47 34.89039584372898 38.0 34.0 40.0 25.5 41.0 48-49 34.529458106436984 38.0 33.0 40.0 24.0 41.0 50-51 34.25938625442041 38.0 33.0 40.0 24.0 41.0 52-53 34.004362782852056 38.0 33.0 40.0 23.0 41.0 54-55 33.706484715683516 37.0 33.0 40.0 22.5 41.0 56-57 32.84063706799297 36.5 31.5 40.0 17.5 41.0 58-59 29.615420861085198 34.5 25.0 38.5 7.5 40.0 60-61 29.325095120191786 34.0 24.5 38.0 7.5 40.0 62-63 31.216622507756057 35.0 29.5 38.5 8.0 40.0 64-65 31.27666721356822 35.0 30.0 39.0 4.5 40.0 66-67 26.28351410463628 31.0 18.0 36.5 2.0 39.5 68-69 18.83827092779815 17.0 7.5 31.0 2.0 36.5 70-71 16.957677616449352 13.5 7.0 28.0 2.0 34.5 72-73 18.57045131581802 17.0 7.5 30.5 2.0 35.0 74-75 19.993498199292734 22.0 7.5 31.5 2.0 35.5 76-77 21.854533904280476 26.5 10.0 33.0 2.0 35.5 78-79 24.029655397782744 30.0 11.5 34.5 2.0 36.0 80-81 25.615810358948213 31.5 19.5 35.0 2.0 36.0 82-83 26.76545211583104 32.5 25.5 35.0 2.0 36.0 84-85 26.820488604560346 33.0 26.0 35.0 2.0 36.0 86-87 26.537026704163324 33.0 25.0 35.0 2.0 36.0 88-89 26.082730159677176 33.0 24.5 35.0 2.0 35.0 90-91 24.205884027943505 31.5 11.0 34.5 2.0 35.0 92-93 22.5054177152713 31.0 2.0 34.0 2.0 35.0 94-95 18.726317241772072 25.0 2.0 34.0 2.0 35.0 96-97 16.11057603649144 8.5 2.0 32.0 2.0 35.0 98-99 16.320052665263706 6.0 2.0 32.0 2.0 35.0 100-101 15.55514666001345 2.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17545581128385734 1101 2 0.26797127701309975 1101 3 0.14192802003486804 1101 4 0.19135140160192066 1101 5 0.3320990533631374 1101 6 0.23909867649894778 1101 7 0.20613632261292025 1101 8 0.3133381521109371 1101 9 0.31828252102226884 1101 10-11 0.14300096533694528 1101 12-13 0.1859559062186591 1101 14-15 0.27299883278497106 1101 16-17 0.3670354758667074 1101 18-19 0.3716822307812535 1101 20-21 0.4971046616826982 1101 22-23 0.3230110619986135 1101 24-25 0.3898887250121206 1101 26-27 0.4559619819599021 1101 28-29 0.2842726824209123 1101 30-31 0.10751067766039313 1101 32-33 0.20962035394897072 1101 34-35 0.32397179074352067 1101 36-37 0.21114016928658685 1101 38-39 0.12918059730069587 1101 40-41 0.23097141226394768 1101 42-43 0.2962457389670803 1101 44-45 0.1599919300068109 1101 46-47 -0.0037194693296100922 1101 48-49 -0.24463238141198218 1101 50-51 -0.11582201162199368 1101 52-53 -0.2244479383335829 1101 54-55 -0.2942787938321558 1101 56-57 -3.619532471214228 1101 58-59 -10.268103393126825 1101 60-61 -11.784862442918158 1101 62-63 -4.374347739718974 1101 64-65 -1.5225377277405059 1101 66-67 -2.3071758435555054 1101 68-69 -5.023258207373667 1101 70-71 -4.433816185657161 1101 72-73 -3.374756964032537 1101 74-75 -6.221518030685111 1101 76-77 -4.984250350934392 1101 78-79 0.7915185032558654 1101 80-81 1.4042940238792774 1101 82-83 0.09976826583952914 1101 84-85 -0.10977766630476893 1101 86-87 -0.12123899916844394 1101 88-89 -0.39773030200178994 1101 90-91 1.086701036007149 1101 92-93 0.6428696101081748 1101 94-95 -8.61424074472826 1101 96-97 -9.188484340882976 1101 98-99 -8.426883844671053 1101 100-101 -9.199262778693013 1102 1 0.26788905008806196 1102 2 0.263853351927434 1102 3 0.15040462407598554 1102 4 0.09797582556505091 1102 5 0.2239695484594435 1102 6 0.2602229823006681 1102 7 0.3090933118602379 1102 8 0.21847078829195965 1102 9 0.2288107025181958 1102 10-11 0.15186233246443948 1102 12-13 0.24670564035548637 1102 14-15 0.24915903011451945 1102 16-17 0.36134600404820105 1102 18-19 0.23887693124208198 1102 20-21 0.33996328155744493 1102 22-23 0.36972543633395816 1102 24-25 0.3886746165399515 1102 26-27 0.22516690338256495 1102 28-29 0.28164949915257154 1102 30-31 0.28263917819212026 1102 32-33 0.15381272072198016 1102 34-35 0.16972684214373857 1102 36-37 -0.13059591720756458 1102 38-39 0.06448574913810745 1102 40-41 -0.11085107976015962 1102 42-43 0.009635642074727002 1102 44-45 -0.30462611324026057 1102 46-47 -0.12931470742485374 1102 48-49 -0.06018980785650285 1102 50-51 -0.09094091153928474 1102 52-53 -0.20007419379854952 1102 54-55 -0.518287715018495 1102 56-57 -3.9897506267146383 1102 58-59 -9.967992616806374 1102 60-61 -8.173338751555757 1102 62-63 -2.7813458491364393 1102 64-65 -1.3353316542441114 1102 66-67 -2.490006252393975 1102 68-69 -5.116616059199259 1102 70-71 -4.800933342693675 1102 72-73 -3.671065158526215 1102 74-75 -4.374744575645526 1102 76-77 -4.55677856197067 1102 78-79 0.8617300121770519 1102 80-81 1.225560123371185 1102 82-83 0.0578194888101109 1102 84-85 -0.30577716411878697 1102 86-87 -0.372010740867605 1102 88-89 -0.4105862632448485 1102 90-91 0.8531564891561487 1102 92-93 0.46853205250448937 1102 94-95 -8.579544124144544 1102 96-97 -9.361323464125327 1102 98-99 -8.516786952316096 1102 100-101 -9.31208403002233 1103 1 0.2636790671258069 1103 2 0.2706915511246386 1103 3 0.24128329513203894 1103 4 0.19203607853221882 1103 5 0.27452110928995666 1103 6 0.3111752188654009 1103 7 0.3349827397963594 1103 8 0.42801589499443793 1103 9 0.34673498563350336 1103 10-11 0.39748070868090224 1103 12-13 0.3811161055724952 1103 14-15 0.5105770251475903 1103 16-17 0.5026242679310826 1103 18-19 0.5736301366479566 1103 20-21 0.4978991878787369 1103 22-23 0.40322246916770865 1103 24-25 0.552813055420657 1103 26-27 0.5821193493093872 1103 28-29 0.45839731167964004 1103 30-31 0.39805653070013847 1103 32-33 0.4640303129134935 1103 34-35 0.4189904651187035 1103 36-37 0.6518144180605603 1103 38-39 0.609218126625187 1103 40-41 0.598710451037725 1103 42-43 0.46165183514989394 1103 44-45 0.32249097070671695 1103 46-47 0.10399732872981815 1103 48-49 0.05877298344558568 1103 50-51 -0.05735868999087046 1103 52-53 0.24908835782003536 1103 54-55 0.12544622185522059 1103 56-57 0.5518212855554196 1103 58-59 -8.22951150271125 1103 60-61 -7.506526975298865 1103 62-63 -2.298455063956389 1103 64-65 -0.45755868938806543 1103 66-67 -1.9207360958777642 1103 68-69 -3.6347796648477058 1103 70-71 -3.747098767626534 1103 72-73 -2.8428260020388763 1103 74-75 -3.9579185411080005 1103 76-77 -0.6424821990956211 1103 78-79 3.202398960168619 1103 80-81 2.6912897141765804 1103 82-83 1.1442690686420391 1103 84-85 0.7094948983144356 1103 86-87 0.6369837074271736 1103 88-89 0.5814226021569837 1103 90-91 1.743474936122567 1103 92-93 1.505354693932194 1103 94-95 -6.685092580640545 1103 96-97 -8.71446425652476 1103 98-99 -8.06577262906102 1103 100-101 -9.21485573148642 1104 1 0.1555957794864078 1104 2 0.17491693903892624 1104 3 0.21070188669360945 1104 4 0.1940943028961044 1104 5 0.33553213756694333 1104 6 0.14036983145949478 1104 7 0.2835943098642275 1104 8 0.25205005832332716 1104 9 0.3418988120134898 1104 10-11 0.17009194466860578 1104 12-13 0.2342163332553966 1104 14-15 0.28635784676693277 1104 16-17 0.294814780780456 1104 18-19 0.42706662328656364 1104 20-21 0.3156511914519413 1104 22-23 0.39529750647938044 1104 24-25 0.23910233253652535 1104 26-27 0.2329638095701867 1104 28-29 0.27228251882750243 1104 30-31 0.35893301908176767 1104 32-33 0.30367727732306093 1104 34-35 0.2597182878238087 1104 36-37 -0.01593595142484361 1104 38-39 -0.08365725057674211 1104 40-41 0.11278557550074453 1104 42-43 -8.772606911762182E-4 1104 44-45 0.08462752234570559 1104 46-47 -0.03770150525775762 1104 48-49 -0.007356189379173372 1104 50-51 -0.04250029277680767 1104 52-53 -0.1140747640865456 1104 54-55 -0.07051540250066779 1104 56-57 0.17171394414364016 1104 58-59 -8.932353181391488 1104 60-61 -7.6435198179943065 1104 62-63 -2.6100812355065557 1104 64-65 -0.8365007446347583 1104 66-67 -1.866483654732157 1104 68-69 -3.2671186254952396 1104 70-71 -3.649489801205222 1104 72-73 -2.9088734704737504 1104 74-75 -3.5274302756740425 1104 76-77 -0.535554937790458 1104 78-79 3.110824679984294 1104 80-81 2.4538464079448907 1104 82-83 0.7778900619495417 1104 84-85 0.5008934232778479 1104 86-87 0.45960638202945603 1104 88-89 0.4374961433704918 1104 90-91 2.007152069424187 1104 92-93 1.7814971936507682 1104 94-95 -6.023188977295385 1104 96-97 -7.580210689674228 1104 98-99 -5.63846502474267 1104 100-101 -5.326246775959028 1105 1 0.23970760509627453 1105 2 0.2856924323872043 1105 3 0.25145142210554283 1105 4 0.28510636743697404 1105 5 0.31210583745779985 1105 6 0.3224972023335084 1105 7 0.23380595553839356 1105 8 0.24940264379278432 1105 9 0.234106269020657 1105 10-11 0.35622399092092394 1105 12-13 0.3818657388776501 1105 14-15 0.4809221664035235 1105 16-17 0.5163336887772658 1105 18-19 0.5060875223750898 1105 20-21 0.4393680434622098 1105 22-23 0.6294237121960293 1105 24-25 0.5843026953576853 1105 26-27 0.491741205517215 1105 28-29 0.699506642009716 1105 30-31 0.5453239072561615 1105 32-33 0.5224908816415237 1105 34-35 0.49792881258708377 1105 36-37 0.6599829005264297 1105 38-39 0.7167435488097027 1105 40-41 0.6571600039836838 1105 42-43 0.716829812846477 1105 44-45 0.760336940946928 1105 46-47 0.7358125503748241 1105 48-49 0.5087757093848779 1105 50-51 0.7376407797579247 1105 52-53 0.7315555270553062 1105 54-55 0.6180833851457166 1105 56-57 1.072082296109663 1105 58-59 -7.551859611880264 1105 60-61 -7.002731198189583 1105 62-63 -1.4768097243416953 1105 64-65 0.685337475476743 1105 66-67 -0.903686465858673 1105 68-69 -2.783364827672166 1105 70-71 -3.406967825452295 1105 72-73 -2.3272125312684153 1105 74-75 -3.079557794036333 1105 76-77 0.6752308797370468 1105 78-79 4.640814577037478 1105 80-81 4.08906587049762 1105 82-83 2.54741308486922 1105 84-85 2.2907642972933644 1105 86-87 2.1806883560125243 1105 88-89 2.0657478911065468 1105 90-91 3.1884807912907966 1105 92-93 2.4576638259035057 1105 94-95 -5.850952170121555 1105 96-97 -7.166393250660629 1105 98-99 -4.861635063974552 1105 100-101 -4.2822502861799645 1106 1 -0.01578266437633502 1106 2 0.019111102307135752 1106 3 0.026790534913118336 1106 4 -0.05230895063436236 1106 5 0.020659443929822885 1106 6 0.15343525783001866 1106 7 0.23265491286085904 1106 8 0.2647039250738814 1106 9 0.11295257690397165 1106 10-11 0.14669783233042466 1106 12-13 0.08776701800223208 1106 14-15 0.21081531082568716 1106 16-17 0.33878949551608173 1106 18-19 0.23755858495422189 1106 20-21 0.13103917720651026 1106 22-23 0.026863268014466257 1106 24-25 0.056426783072602404 1106 26-27 0.04300226923622574 1106 28-29 0.06260188010494971 1106 30-31 0.07837200227860563 1106 32-33 0.060578109804446 1106 34-35 0.11467011025774099 1106 36-37 0.06834426862286591 1106 38-39 0.3226281149024288 1106 40-41 -0.014582764741845722 1106 42-43 0.2879920925348358 1106 44-45 0.24001674390259353 1106 46-47 0.27637746625512705 1106 48-49 0.0810424355098931 1106 50-51 -0.04600266888763116 1106 52-53 0.15562579726729098 1106 54-55 -0.031160716001245703 1106 56-57 0.7174296422034345 1106 58-59 -8.78828990082317 1106 60-61 -7.5654208564427385 1106 62-63 -2.212023504814095 1106 64-65 -0.3936895565044516 1106 66-67 0.22208891869509628 1106 68-69 -2.5494792326112155 1106 70-71 -2.228183633053721 1106 72-73 -0.8723991395858057 1106 74-75 -1.235644803466485 1106 76-77 1.8040044050622974 1106 78-79 4.0929392500936075 1106 80-81 3.3113503494053127 1106 82-83 1.7004990653311474 1106 84-85 1.217289149648746 1106 86-87 0.8864850824148043 1106 88-89 0.9714091803359608 1106 90-91 2.2918670671959944 1106 92-93 1.6164241502441499 1106 94-95 -6.311241390293031 1106 96-97 -7.546462613593745 1106 98-99 -5.273378125370682 1106 100-101 -4.882083694991506 1107 1 0.05368257802555476 1107 2 -0.041970030056941 1107 3 0.05382678241300454 1107 4 0.021731906944971513 1107 5 0.09146917989095726 1107 6 0.07167440497500621 1107 7 0.06618825510069115 1107 8 0.23457170234178903 1107 9 0.03349889358409541 1107 10-11 0.08816724916795948 1107 12-13 0.09341063814407136 1107 14-15 0.12426315071012795 1107 16-17 0.1510076581835449 1107 18-19 0.10547080563394928 1107 20-21 0.2130854251517249 1107 22-23 0.10429808118136918 1107 24-25 0.052385363996826584 1107 26-27 -0.010507761220125644 1107 28-29 -0.21582949244406535 1107 30-31 -0.08521243613781593 1107 32-33 -0.08347535240201154 1107 34-35 0.1762947324577553 1107 36-37 0.114997846613754 1107 38-39 0.3241809430051248 1107 40-41 0.09136309538357779 1107 42-43 0.1721015494903284 1107 44-45 -0.06091204866707045 1107 46-47 -0.11587117211598752 1107 48-49 -0.14578799449957813 1107 50-51 -0.1641155761419668 1107 52-53 -0.2636817420810189 1107 54-55 -0.1173165370736271 1107 56-57 0.5839013340696724 1107 58-59 -8.6152891670054 1107 60-61 -7.642883938051408 1107 62-63 -2.146468213871806 1107 64-65 -0.5023226611731992 1107 66-67 0.7688716408685892 1107 68-69 -2.6554429057273445 1107 70-71 -2.5011378982000867 1107 72-73 -1.0388108094181518 1107 74-75 -0.9257682745987914 1107 76-77 2.0400526705223285 1107 78-79 4.137843088986724 1107 80-81 2.9514410020384396 1107 82-83 1.4804919788587583 1107 84-85 1.1327115208288774 1107 86-87 0.9502517167470153 1107 88-89 0.7912307793616691 1107 90-91 1.8222722933725706 1107 92-93 1.2863256172988287 1107 94-95 -6.939150861576358 1107 96-97 -8.533627385693954 1107 98-99 -6.657075172867474 1107 100-101 -5.875233631968371 1108 1 -0.09598729970836217 1108 2 0.02713888919066676 1108 3 -0.055421628358072184 1108 4 -0.007713234445567707 1108 5 0.019163388077075183 1108 6 0.08177104390449585 1108 7 -0.08424621725996673 1108 8 0.021017699249654243 1108 9 -0.11291349645507154 1108 10-11 -0.067885631777024 1108 12-13 -0.02582047046058733 1108 14-15 -0.09098634959870111 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0.014658254671843451 2211 44-45 -0.020669589631417296 2211 46-47 0.24379708696177715 2211 48-49 0.13005165597767387 2211 50-51 0.2399453517559209 2211 52-53 0.39380069510944793 2211 54-55 0.13395885188039358 2211 56-57 0.7189652572909822 2211 58-59 3.9966876532546927 2211 60-61 3.9036723719785478 2211 62-63 1.3958200864732078 2211 64-65 1.0043977113130644 2211 66-67 5.871635651507859 2211 68-69 12.326343216624256 2211 70-71 7.599897697127432 2211 72-73 10.344006621239274 2211 74-75 9.94477876800861 2211 76-77 7.608508951288702 2211 78-79 5.939199838156814 2211 80-81 4.220865590530956 2211 82-83 2.4648236601354725 2211 84-85 1.8856832656280673 2211 86-87 1.819543221174765 2211 88-89 1.9691039692599546 2211 90-91 3.614883145502013 2211 92-93 5.087459441340819 2211 94-95 8.263390783111557 2211 96-97 10.170657772077766 2211 98-99 9.182048373505983 2211 100-101 8.384732109136523 2212 1 -0.15289318984653733 2212 2 -0.10982332904538339 2212 3 -0.13425969229433932 2212 4 -0.1147540947292498 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0.2630333569964378 2212 66-67 5.223688055801404 2212 68-69 12.002781549629677 2212 70-71 13.609252126343726 2212 72-73 11.470942556576993 2212 74-75 9.7529518784726 2212 76-77 7.192377119615269 2212 78-79 5.4387635294466214 2212 80-81 3.7009841985806666 2212 82-83 1.9631459756280094 2212 84-85 1.511779805020801 2212 86-87 1.5139934998844886 2212 88-89 1.4490448424163027 2212 90-91 2.937917882984067 2212 92-93 4.651851335153172 2212 94-95 7.736184978655373 2212 96-97 9.018502307384994 2212 98-99 8.310410063829421 2212 100-101 7.435283382607958 2213 1 -0.14884301517752263 2213 2 -0.16477128228379456 2213 3 -0.08478758129778186 2213 4 0.016084586760406694 2213 5 -0.025728952915038406 2213 6 0.07178855825171837 2213 7 -0.17105876759352157 2213 8 -0.10664781594675787 2213 9 -0.1528688221780925 2213 10-11 -0.2584978032099414 2213 12-13 -0.18669863383090757 2213 14-15 -0.18309300943500517 2213 16-17 -0.13557471954850087 2213 18-19 -0.41335160914986346 2213 20-21 -0.23635466647837688 2213 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52-53 -0.1657764240577393 2313 54-55 -0.056074032076956826 2313 56-57 0.7323749007115197 2313 58-59 3.5941457195408226 2313 60-61 3.472532255074743 2313 62-63 0.7411978518882734 2313 64-65 -0.09167583748702768 2313 66-67 -2.1977104416646256 2313 68-69 -4.347244449796005 2313 70-71 -3.040106037087888 2313 72-73 -2.3210011139210316 2313 74-75 -3.4366757088583064 2313 76-77 0.3893825290480173 2313 78-79 3.059755931405501 2313 80-81 2.4406746501945698 2313 82-83 0.86647681249908 2313 84-85 0.43403220899755013 2313 86-87 0.5684993555760336 2313 88-89 0.5539556294616013 2313 90-91 2.119755343887917 2313 92-93 3.448606645632882 2313 94-95 5.69901976976745 2313 96-97 1.1536026962122268 2313 98-99 3.1407929150739005 2313 100-101 4.259423655330293 2314 1 -0.44704421349699786 2314 2 -0.4106631657315596 2314 3 -0.44080947141573645 2314 4 -0.26872333745568255 2314 5 -0.23228243302556706 2314 6 -0.30286274466654817 2314 7 -0.2890314107318659 2314 8 -0.5646334853748982 2314 9 -0.4351991890549485 2314 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32-33 -0.7159862644458599 2315 34-35 -0.6820003241326091 2315 36-37 -0.5758730444519031 2315 38-39 -0.8327917684262971 2315 40-41 -0.7192380821020805 2315 42-43 -0.573644022250015 2315 44-45 -0.5892182287750032 2315 46-47 -0.3172440594935466 2315 48-49 -0.28613633400794214 2315 50-51 -0.2818903650911935 2315 52-53 -0.2930581906759926 2315 54-55 -0.30213822243457145 2315 56-57 0.39600757000667386 2315 58-59 3.400235330110096 2315 60-61 3.2302326770156675 2315 62-63 0.5487478276785467 2315 64-65 -0.2825115917929004 2315 66-67 -2.226203559185546 2315 68-69 -4.444982569737903 2315 70-71 -3.424655044489146 2315 72-73 -3.0076918173864797 2315 74-75 -0.7062873156923644 2315 76-77 1.8662942327209784 2315 78-79 3.4140133845423897 2315 80-81 2.453116719780237 2315 82-83 0.88390262698341 2315 84-85 0.5647975821731741 2315 86-87 0.5510718118256044 2315 88-89 0.7364960942883307 2315 90-91 2.1337770980554467 2315 92-93 3.52302561222346 2315 94-95 6.215225587018612 2315 96-97 3.8797284484429806 2315 98-99 6.023669722156972 2315 100-101 6.360061754520993 2316 1 -0.641193794712926 2316 2 -0.5146512248492812 2316 3 -0.6653145469617598 2316 4 -0.3328595647465775 2316 5 -0.6641462594187857 2316 6 -0.6634656940337962 2316 7 -0.5998159753967869 2316 8 -0.5377914250166853 2316 9 -0.9643189899635516 2316 10-11 -0.8416189747452663 2316 12-13 -0.7058810098553536 2316 14-15 -0.9079677568853768 2316 16-17 -0.7943903941827983 2316 18-19 -0.6065482204600698 2316 20-21 -0.6893345174757783 2316 22-23 -1.0585856559215188 2316 24-25 -0.9246905999570174 2316 26-27 -1.0011424918658207 2316 28-29 -0.665979833357973 2316 30-31 -0.7328382978955048 2316 32-33 -0.8179886780232621 2316 34-35 -0.7723435065140833 2316 36-37 -0.8179750697325048 2316 38-39 -0.9190314266306885 2316 40-41 -1.074947166358946 2316 42-43 -1.0930249307937316 2316 44-45 -1.0137629537216029 2316 46-47 -0.971952010726298 2316 48-49 -0.9531305154570404 2316 50-51 -0.8417193492642667 2316 52-53 -0.8715901868048235 2316 54-55 -0.7074230127660996 2316 56-57 -0.20296606947921703 2316 58-59 3.03360539820957 2316 60-61 3.0075904883907647 2316 62-63 0.2845983039027473 2316 64-65 -0.5968970147295565 2316 66-67 -2.3452419049703437 2316 68-69 -3.052377912536709 2316 70-71 -3.292688716549481 2316 72-73 -0.9693880784069115 2316 74-75 5.127583023860801 2316 76-77 5.329977711813143 2316 78-79 4.304461165113388 2316 80-81 2.6522545592448843 2316 82-83 1.0398350704207218 2316 84-85 0.6764550430189296 2316 86-87 0.6180161284114227 2316 88-89 0.3889534878607783 2316 90-91 1.925599408008356 2316 92-93 3.5093290030341606 2316 94-95 5.8156258336874025 2316 96-97 2.819805045593897 2316 98-99 4.89319196365634 2316 100-101 4.905614339822369 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 34.0 5 80.0 6 194.0 7 518.0 8 1101.0 9 1780.0 10 2990.0 11 4486.0 12 6352.0 13 8545.0 14 10723.0 15 14361.0 16 18890.0 17 24553.0 18 25630.0 19 23585.0 20 24580.0 21 25088.0 22 25495.0 23 25545.0 24 26689.0 25 27958.0 26 34613.0 27 48225.0 28 67704.0 29 89547.0 30 106681.0 31 116339.0 32 119597.0 33 117577.0 34 118133.0 35 123872.0 36 119465.0 37 84030.0 38 28667.0 39 1338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.77770242777702 20.77851620778516 15.005357385053575 22.43842397938424 2 15.401707918073093 22.3331918431113 39.56120853443705 22.703891704378556 3 18.207451933018433 27.190328229737087 31.4255921972809 23.17662763996358 4 12.448066951597857 18.19514351419955 38.22475755537717 31.13203197882542 5 12.397539732399085 39.00355905687029 34.83621961131245 13.762681599418174 6 29.496547740437812 38.450252749875254 17.98788590458421 14.065313605102729 7 26.103475581975573 34.66693695355043 22.115885275421434 17.113702189052567 8 25.45112720783852 35.30195827446216 20.64127319027073 18.605641327428586 9 26.461040722018524 16.21260278133339 20.94752728179984 36.37882921484824 10-11 23.930084286117197 27.41505624498297 28.298663842665917 20.35619562623392 12-13 25.353429479530515 25.306716855053914 28.624635248302344 20.715218417113228 14-15 22.414542974365858 26.013675564834866 27.574151684122995 23.997629776676284 16-17 20.827973074807005 29.345165394272648 28.09809383274502 21.728767698175325 18-19 20.7547809414558 27.607593640824124 31.06250902560118 20.575116392118893 20-21 21.86594900527195 26.857657344933074 30.814162399930574 20.462231249864406 22-23 21.44790830494869 26.948845269346755 30.560598952118546 21.04264747358601 24-25 21.170426617400295 27.121103747521147 30.846488923541077 20.861980711537484 26-27 21.202238141146896 27.15624139815346 30.803322634839663 20.83819782585998 28-29 20.91752903380868 27.083791678630252 31.058675090841355 20.940004196719713 30-31 21.160969206663243 27.21149341276035 30.854391866008267 20.773145514568135 32-33 20.95572194775833 27.32254590240461 30.63722141670376 21.084510733133296 34-35 21.24531383531752 27.21031544384281 30.56177703229288 20.982593688546793 36-37 21.494127798300145 27.02400353421275 30.488794245096436 20.993074422390677 38-39 21.260786632819865 27.16290775796143 30.712682847718565 20.863622761500146 40-41 21.288868395006684 27.042966916970258 30.721550911859 20.946613776164057 42-43 21.07684472016811 27.336988259198193 30.365993648613387 21.220173372020305 44-45 21.2351904227866 27.466147719965594 30.04945715890254 21.249204698345263 46-47 21.26328104667864 27.440568874821818 29.56723289712675 21.72891718137279 48-49 21.38891980066878 27.61910610370435 29.396164013983583 21.59581008164329 50-51 21.49250905898202 27.886669514154942 29.151890656450085 21.468930770412946 52-53 21.506455637548058 27.949372550429686 29.091312450686335 21.452859361335918 54-55 21.3774474323117 27.908512516051655 29.086074862276284 21.627965189360353 56-57 21.314100389453543 28.219844489978698 28.90539128546809 21.560663835099668 58-59 20.689796473092663 27.467493131197372 30.378854673688338 21.463855722021624 60-61 20.951565503641405 27.7743590162267 29.880588271912984 21.393487208218907 62-63 21.575054434436126 27.72171333129257 29.135873030724312 21.567359203546992 64-65 21.439915658014947 27.719291703603012 29.128833399154885 21.71195923922716 66-67 21.66155069609764 27.401618257759218 29.52979345875602 21.40703758738712 68-69 21.696690675457738 26.56622207871342 30.75822991151641 20.978857334312433 70-71 22.62922148506316 26.59037556241349 29.690504252894144 21.0898986996292 72-73 21.543574417874886 26.909195351905595 29.894706390629246 21.652523839590273 74-75 21.504686241261588 27.237442404937674 29.511541676195733 21.746329677605004 76-77 20.090947618037763 27.78731125005507 29.968626623351124 22.153114508556047 78-79 21.095630771748134 27.604816139248058 29.2248088441334 22.07474424487041 80-81 20.80363386515984 28.227941341049263 28.689804951731368 22.278619842059534 82-83 21.106655669873838 28.17992570046079 28.349928524236507 22.36349010542887 84-85 20.989994626963828 27.90107511572367 28.28481498619452 22.824115271117982 86-87 20.96945712746689 27.860324300429944 28.556851900001323 22.613366672101844 88-89 21.150155087374362 27.6268750317802 28.45583823454635 22.76713164629909 90-91 20.907237658351836 27.733847840755736 28.788098308737105 22.570816192155327 92-93 20.45246023301383 27.900029746590928 28.769261087219245 22.878248933175996 94-95 19.994277306267822 27.61921907820136 29.316928734322918 23.0695748812079 96-97 20.16728746267813 27.36469507920061 29.211709708753258 23.256307749368002 98-99 20.208521330619917 27.325335239320193 28.898252030370696 23.5678913996892 100-101 19.560976990194572 27.549133692946427 29.339232251846 23.550657065013006 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1200.0 1 912.5 2 614.5 3 655.0 4 753.0 5 879.0 6 1092.5 7 1592.5 8 2145.5 9 2507.5 10 2704.0 11 2705.0 12 2710.0 13 2836.0 14 2996.0 15 3175.0 16 3387.0 17 3557.0 18 3748.5 19 3967.0 20 4266.5 21 4733.5 22 5364.0 23 6096.5 24 7109.5 25 8457.0 26 10177.0 27 12276.0 28 14382.0 29 16902.5 30 19855.5 31 22988.5 32 26678.0 33 30390.0 34 33927.0 35 37785.5 36 41506.0 37 44426.5 38 46423.5 39 47530.0 40 48105.0 41 49055.0 42 50377.0 43 51820.5 44 53257.5 45 54255.5 46 55072.0 47 56102.0 48 57470.0 49 56532.0 50 53402.5 51 50518.0 52 47630.0 53 45171.5 54 42482.5 55 38806.0 56 34556.0 57 30335.5 58 26574.5 59 22513.5 60 18169.5 61 14029.0 62 10437.0 63 7627.0 64 5395.0 65 3743.0 66 2537.0 67 1706.5 68 1074.0 69 614.0 70 342.5 71 182.5 72 106.5 73 70.0 74 42.0 75 25.0 76 12.0 77 5.0 78 3.5 79 3.0 80 1.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02549194020784067 2 0.013423947237107587 3 2.0339313995617554E-4 4 0.0 5 0.06711973618553793 6 0.0 7 0.0 8 2.7119085327490075E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 6.440782765278893E-4 16-17 3.3898856659362594E-5 18-19 3.3898856659362594E-4 20-21 0.0 22-23 0.0 24-25 6.779771331872519E-5 26-27 1.3559542663745038E-4 28-29 9.830668431215152E-4 30-31 0.00535601935217929 32-33 0.0054577159221573775 34-35 0.0015254485496713167 36-37 0.09359474323650012 38-39 0.08657767990801206 40-41 0.09257777753671925 42-43 0.09759480832230491 44-45 0.10023891914173519 46-47 0.09495069750287463 48-49 0.08461154622176903 50-51 0.07881484173301803 52-53 0.06732312932549411 54-55 0.056204304341223185 56-57 0.07566224806369731 58-59 0.7286220250363395 60-61 0.3488531338815004 62-63 0.002474616536133469 64-65 0.001932234829583668 66-67 1.1056790076584297 68-69 2.3646825439871564 70-71 2.326139543965461 72-73 1.8032496799947932 74-75 1.2817157702904998 76-77 0.7346899203783654 78-79 0.5587209554596142 80-81 0.04223797539756579 82-83 3.3898856659362594E-5 84-85 0.001254257696396416 86-87 0.0011186622697589657 88-89 6.779771331872519E-5 90-91 0.32759855075608013 92-93 0.05813653917080685 94-95 0.9576765994836527 96-97 1.6555523615299506 98-99 0.6385527628924131 100-101 0.5757720803592736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1474976.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.90968106695044 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0247199615532 39.166243242361006 2 13.764125779511271 14.56511010318664 3 5.094702679234044 8.086772816676337 4 2.3580407801131074 4.990527424745901 5 1.2939361191094847 3.423087369154522 6 0.8002474662022782 2.540450292683864 7 0.5244198207542451 1.94228198229061 8 0.3745259706581684 1.5852839727050987 9 0.27579748465326226 1.313312125686414 >10 1.3644902351264194 12.83216084176601 >50 0.0808770902959094 2.9125134440816445 >100 0.041295264906083116 3.6935682349458148 >500 0.0015389170268097756 0.5713223236903173 >1k 8.977015989723691E-4 0.7589358896052494 >5k 2.564861711349626E-4 0.9299933216745236 >10k+ 1.282430855674813E-4 0.688436614746076 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10146 0.6878755993317857 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7561 0.5126185104028812 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6145 0.4166169483435663 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1885 0.127798689605797 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1875 0.12712071247260973 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1702 0.11539170806847025 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1663 0.11274759724903997 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 0.10895092530319138 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.779771331872519E-5 0.0 0.0 0.0 0.0 3 6.779771331872519E-5 0.0 0.0 0.0 0.0 4 6.779771331872519E-5 0.0 0.0 0.0 0.0 5 6.779771331872519E-5 0.0 0.0 0.0 0.0 6 1.3559542663745038E-4 0.0 0.0 0.0 0.0 7 1.3559542663745038E-4 0.0 0.0 0.0 0.0 8 1.3559542663745038E-4 0.0 0.0 0.0 0.0 9 1.3559542663745038E-4 0.0 0.0 0.0 0.0 10-11 1.3559542663745038E-4 0.0 0.0 1.0169656997808778E-4 0.0 12-13 1.3559542663745038E-4 0.0 0.0 1.3559542663745038E-4 0.0 14-15 1.3559542663745038E-4 0.0 0.0 1.3559542663745038E-4 0.0 16-17 1.3559542663745038E-4 0.0 0.0 2.3729199661553816E-4 0.0 18-19 1.3559542663745038E-4 0.0 0.0 2.7119085327490075E-4 0.0 20-21 1.3559542663745038E-4 0.0 0.0 5.084828498904389E-4 0.0 22-23 1.3559542663745038E-4 0.0 0.0 9.152691298027901E-4 0.0 24-25 2.7119085327490075E-4 0.0 0.0 0.001322055409715141 0.0 26-27 2.7119085327490075E-4 0.0 0.0 0.0020339313995617558 0.0 28-29 3.7288742325298856E-4 0.0 0.0 0.004474649079035863 0.0 30-31 4.0678627991235113E-4 0.0 0.0 0.01257647582062352 0.0 32-33 4.0678627991235113E-4 0.0 0.0 0.030814060703360596 0.0 34-35 4.0678627991235113E-4 0.0 0.0 0.05433986722495823 0.0 36-37 5.423817065498015E-4 0.0 0.0 0.09447611350964355 0.0 38-39 7.118759898466144E-4 0.0 0.0 0.15040922699759182 0.0 40-41 7.45774846505977E-4 0.0 0.0 0.21881711973618553 0.0 42-43 8.474714164840648E-4 0.0 0.0 0.2871911136181199 0.0 44-45 0.0010169656997808779 0.0 0.0 0.3692263467337774 0.0 46-47 0.0011186622697589655 0.0 0.0 0.4623804048337058 0.0 48-49 0.0011525611264183281 0.0 0.0 0.5633311999652876 0.0 50-51 0.0011525611264183281 0.0 0.0 0.6644175905235068 0.0 52-53 0.0011525611264183281 0.0 0.0 0.7603174560128436 0.0 54-55 0.0011525611264183281 0.0 0.0 0.8670649556331764 0.0 56-57 0.0011525611264183281 0.0 0.0 0.9674394702015491 0.0 58-59 0.0011525611264183281 0.0 0.0 1.0417457639988719 0.0 60-61 0.0011525611264183281 0.0 0.0 1.1054078168051547 0.0 62-63 0.0012881565530557786 6.779771331872519E-5 0.0 1.1618833119996528 0.0 64-65 0.0012881565530557786 6.779771331872519E-5 0.0 1.2241894105395614 0.0 66-67 0.0012881565530557786 6.779771331872519E-5 0.0 1.2783258846245633 0.0 68-69 0.0013559542663745038 6.779771331872519E-5 0.0 1.3449371379602109 0.0 70-71 0.0013559542663745038 6.779771331872519E-5 0.0 1.4520575250037966 0.0 72-73 0.0013898531230338664 6.779771331872519E-5 0.0 1.5977887097823964 0.0 74-75 0.001423751979693229 6.779771331872519E-5 0.0 1.795181752109865 0.0 76-77 0.001423751979693229 6.779771331872519E-5 0.0 2.054677499837285 0.0 78-79 0.001423751979693229 6.779771331872519E-5 0.0 2.3766827392445706 0.0 80-81 0.0015593474063306794 6.779771331872519E-5 0.0 2.7572652029592346 0.0 82-83 0.0015593474063306794 6.779771331872519E-5 0.0 3.1502546482112255 0.0 84-85 0.0015593474063306794 6.779771331872519E-5 0.0 3.5004976352157593 0.0 86-87 0.0015593474063306794 6.779771331872519E-5 0.0 3.819316382097065 0.0 88-89 0.0015593474063306794 6.779771331872519E-5 0.0 4.121524689215283 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5720 0.0 36.548004 1 GTATCAA 9145 0.0 34.522194 1 TATCAAC 11550 0.0 27.164845 2 ATCAACG 11545 0.0 27.13298 3 TCAACGC 11615 0.0 27.050692 4 CAACGCA 11770 0.0 26.614296 5 AACGCAG 12105 0.0 25.938105 6 GTACATG 7295 0.0 25.616858 1 TACATGG 7310 0.0 24.913656 2 ACATGGG 7225 0.0 24.224756 3 ACGCAGA 13380 0.0 23.325485 7 CGCAGAG 13355 0.0 23.157345 8 AGTACTT 7430 0.0 22.240408 12-13 GAGTACT 7070 0.0 22.172558 12-13 CATGGGA 5300 0.0 21.985909 4 GCAGAGT 13785 0.0 21.58073 9 GTACTTT 7835 0.0 20.248348 14-15 ATGGGAT 2115 0.0 19.628887 5 AGAGTAC 12745 0.0 19.013708 10-11 TACTTTT 8840 0.0 18.586348 14-15 >>END_MODULE