FastQCFastQC Report
Fri 27 May 2016
SRR939550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134569
Sequences flagged as poor quality0
Sequence length101
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47623.53870505094041No Hit
NTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38002.823830154047366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36282.696014683916801No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28032.0829462952091493No Hit
NTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24721.836975826527655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19431.4438689445563242No Hit
NGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17571.30564988964769No Hit
NATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16111.1971553626763964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13601.0106339498695838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12400.9214603660575615No Hit
NTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.6145546151045188No Hit
NCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.5045738617363583No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.4689044282115495No Hit
NAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5480.40722603274156755No Hit
NAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.36412546723242356No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.34629075047001906No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4360.32399735451701356No Hit
NCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4100.30467641135774215No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.30244707176244157No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3590.26677763823763273No Hit
NCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3270.2429980158877602No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2970.22070461993475465No Hit
NTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.21624594074415357No Hit
NCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.20509924276765082No Hit
NTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.18503518640994582No Hit
NTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2450.18206273361621178No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.170916035639709No Hit
NGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.16422801685380733No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.1538244320757381No Hit
NTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1930.14342084729766885No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1880.13970528130550125No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1740.12930169652743204No Hit
NCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1680.12484301733683091No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.12335679093996388No Hit
NCGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1640.12187056454309685No Hit
NGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1610.1196412249477963No Hit
NAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1570.11666877215406223No Hit
NGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1540.11443943255876167No Hit
NTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1490.1107238665665941No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.1107238665665941No Hit
NGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.10998075336816059No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1460.10849452697129353No Hit
NATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1450.10775141377286002No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1370.10180650818539189No Hit
NTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1350.10032028178852484No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAGGG156.7318924E-494.546572
CTGTGCC200.002122237870.8044059
CCCCTAT1000.001126762740.830731
AATATGG250.001655009537.80454376-77
GAGCTAT500.0016581237.7764056
AATGGCG250.001661076137.77640540-41
TGGGAGC500.0016581237.7764056
AGAGTGC250.001664115937.76235210-11
GAGTGAA550.002647063734.3421866
CCTGTGG300.004053855334.025611
CATGGGG753.151283E-431.5155224
TGGCGTA300.004061250531.4803442-43
TTGGGCG300.004061250531.4803444-45
CTATAGA600.004061483331.4686289
GCTATAG600.004061483331.4686288
CGCAGAA902.5022822E-531.4686269
GTACATG10200.030.0225961
CATGGGC804.5998412E-429.5458034
ATACTAT804.6300565E-429.5128146
GATGGCA804.6300565E-429.5128147
Produced by FastQC (version 0.11.5)