FastQCFastQC Report
Fri 27 May 2016
SRR939549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5047905
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108660.21525761677369126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86810.17197233307679127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.10885704069311922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97450.045.8633541
GTATCAA166650.040.709531
TATCAAC213750.031.7353922
ATCAACG216350.031.4840453
TCAACGC220500.030.8448354
CAACGCA221550.030.4968435
AACGCAG230800.029.5674486
ACGCAGA253600.026.5410587
CGCAGAG256200.026.0888528
GCAGAGT266850.023.2724349
AGAGTAC224050.021.78032910-11
GAGTACT161000.021.40883812-13
TACATGG141050.021.155142
GTACATG152600.020.5542951
CAGAGTA255300.020.33539210-11
GTACTTT169000.020.31689814-15
AGTACTT167600.019.97980912-13
ACATGGG147050.019.591283
GTAAGGT23650.019.2395634
GTGGTAT55200.018.6079081
Produced by FastQC (version 0.11.5)