FastQCFastQC Report
Fri 27 May 2016
SRR939549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5047905
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159420.3158141843002196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145410.28806009621813405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90960.18019356544942902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118550.046.0590321
GTATCAA186300.035.0590551
TATCAAC238450.027.3253632
ATCAACG236900.027.2787113
TCAACGC239000.026.9993784
CAACGCA244100.026.357645
AACGCAG252850.025.5204726
ACGCAGA286050.022.5087837
CGCAGAG291200.021.9968198
GTGGTAT65100.021.8425881
TGGTATC64350.021.0609552
GAGTACT189550.021.02151112-13
TAAGGTG29800.020.9861225
GTACTTT192950.020.20909114-15
GTAAGGT28750.020.104654
AGAGTAC263150.019.68766810-11
GCAGAGT316750.019.5494029
TACATGG153900.019.490622
CAGAGTA299350.018.81043210-11
AGTACTT196400.018.79263512-13
Produced by FastQC (version 0.11.5)