FastQCFastQC Report
Fri 27 May 2016
SRR939548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4992787
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107150.21460959580290526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79090.15840852013114118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54630.10941784618490634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98750.041.8393941
GTATCAA163950.041.611741
TATCAAC214400.031.4169182
ATCAACG218350.031.2864973
TCAACGC223300.030.6910424
CAACGCA224700.030.1740155
AACGCAG230150.029.2412386
ACGCAGA253100.025.9187977
CGCAGAG258650.025.0434728
GCAGAGT263550.022.6077169
AGAGTAC215900.022.439810-11
TACATGG136550.021.2226662
GTACATG144800.020.8781191
GAGTACT157550.020.5883412-13
AGTACTT166550.020.28427512-13
ACATGGG141900.019.8133623
GTACTTT168250.019.62925314-15
TAAGGTG27450.019.2856775
CAGAGTA250950.018.83195110-11
GTAAGGT24900.018.1956624
Produced by FastQC (version 0.11.5)