FastQCFastQC Report
Fri 27 May 2016
SRR939548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4992787
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155560.3115694701175916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137460.27531717255312516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90460.1811813722476044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111600.045.2118721
GTATCAA181800.037.0747221
TATCAAC233100.028.9890212
ATCAACG231650.028.8799253
TCAACGC235300.028.4191784
CAACGCA240550.027.7733145
AACGCAG248450.026.9283686
ACGCAGA281150.023.7120577
TAAGGTG27900.023.4529195
CGCAGAG286600.023.1618848
GTAAGGT26850.022.978494
GTGGTAT64100.021.6966671
TGGTATC63500.020.9242632
GCAGAGT307950.020.8324139
GAGTACT184450.020.73997312-13
AGAGTAC260050.020.33679810-11
GTACTTT189200.020.15664314-15
AGTACTT190150.019.96868512-13
GGTAAGG32200.019.8923993
AGGTAAG29400.019.6914792
Produced by FastQC (version 0.11.5)