FastQCFastQC Report
Fri 27 May 2016
SRR939547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340648
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138220.5905202320041287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114950.4911033183972985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75190.3212358287106818No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51430.21972547773095313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28140.12022311770073929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91900.047.9457441
GTATCAA156850.040.1905671
TATCAAC200850.031.1737652
ATCAACG208750.029.9091513
TCAACGC213550.029.324814
CAACGCA214400.028.993145
AACGCAG219300.028.3304126
GTACATG139700.027.3470881
TACATGG145450.025.8687292
ACGCAGA241400.025.0664187
CGCAGAG243300.025.0020128
GAGTACT133850.024.75186712-13
ACATGGG145400.023.9912643
GCAGAGT240350.023.7880789
GTACTTT147050.022.6585814-15
AGTACTT142250.022.17878212-13
CATGGGA86900.022.0928424
ATGGGAT37150.021.1919175
ACTTTTT173750.020.73300616-17
CAGAGTA236650.020.71792410-11
Produced by FastQC (version 0.11.5)