##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939547_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2340648 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.04792689887587 31.0 26.0 31.0 10.0 33.0 2 27.14520594296964 31.0 26.0 31.0 16.0 34.0 3 27.60007955062017 31.0 26.0 31.0 16.0 34.0 4 31.3419766662907 35.0 30.0 37.0 19.0 37.0 5 31.629541904634955 35.0 32.0 37.0 19.0 37.0 6 31.69530574439215 35.0 32.0 37.0 17.0 37.0 7 31.149811505190016 35.0 30.0 37.0 17.0 37.0 8 31.068518632447084 35.0 30.0 37.0 17.0 37.0 9 32.05239232896189 35.0 30.0 39.0 17.0 39.0 10-11 31.739566991704862 35.0 30.0 39.0 16.0 39.0 12-13 31.540065827924575 35.0 29.5 39.0 16.0 39.0 14-15 32.214218028511766 36.0 30.0 39.0 16.5 40.5 16-17 32.18708537123053 36.0 30.0 39.0 16.5 40.0 18-19 32.37473404800722 36.0 30.0 39.0 17.0 40.5 20-21 32.20440728379492 36.0 30.0 39.0 16.0 41.0 22-23 32.024178988040916 36.0 30.0 39.0 13.5 41.0 24-25 31.8720668379013 36.0 29.5 39.0 12.5 41.0 26-27 31.834290119659173 36.0 29.5 39.0 13.5 41.0 28-29 31.813103892597262 36.0 30.0 39.0 10.5 41.0 30-31 31.41143585024318 36.0 29.0 39.0 10.0 40.5 32-33 30.77997332362662 35.0 27.0 39.0 9.0 40.0 34-35 30.319764441300016 35.0 26.0 39.0 9.0 40.0 36-37 29.995328857649675 35.0 25.0 38.0 8.0 40.0 38-39 29.547907887046662 35.0 25.0 38.0 8.0 40.0 40-41 29.40178937627529 34.5 25.0 38.0 4.5 40.0 42-43 28.858637864386274 34.0 24.0 38.0 2.0 40.0 44-45 28.577575739709687 34.0 23.5 38.0 2.0 40.0 46-47 27.84081544939692 33.0 22.5 38.0 2.0 40.0 48-49 27.193855077739155 33.0 19.5 38.0 2.0 40.0 50-51 26.685246777815372 32.5 19.0 37.5 2.0 39.5 52-53 27.032854149791 33.0 20.5 37.5 2.0 39.0 54-55 26.894828056162225 33.0 19.0 38.0 2.0 40.0 56-57 26.404363877011836 33.0 16.5 38.0 2.0 40.0 58-59 25.888027161709065 32.0 13.5 37.0 2.0 39.5 60-61 25.311053178436055 31.0 11.0 37.0 2.0 39.0 62-63 24.762898137609756 31.0 8.0 36.0 2.0 39.0 64-65 24.23101636811686 31.0 7.5 36.0 2.0 39.0 66-67 23.778605540004307 30.0 4.5 35.0 2.0 38.5 68-69 23.328276186765375 30.0 2.0 35.0 2.0 38.0 70-71 22.812416262505085 29.0 2.0 34.5 2.0 38.0 72-73 22.14281045249008 29.0 2.0 34.0 2.0 37.0 74-75 21.494299869096082 28.0 2.0 34.0 2.0 36.0 76-77 20.80844535359439 27.0 2.0 34.0 2.0 36.0 78-79 20.045875543866487 26.0 2.0 33.0 2.0 35.0 80-81 19.31570488172506 25.5 2.0 33.0 2.0 35.0 82-83 18.477523959177116 24.5 2.0 32.0 2.0 35.0 84-85 17.67057626776858 21.5 2.0 32.0 2.0 34.0 86-87 17.052576679620344 19.5 2.0 31.0 2.0 34.0 88-89 16.174551449000447 15.5 2.0 31.0 2.0 34.0 90-91 15.320907073596713 7.0 2.0 30.5 2.0 34.0 92-93 14.44475546942556 2.0 2.0 30.0 2.0 34.0 94-95 13.546124833806706 2.0 2.0 29.0 2.0 34.0 96-97 12.520087172441137 2.0 2.0 29.0 2.0 33.5 98-99 11.506990371897015 2.0 2.0 28.0 2.0 33.0 100-101 10.24030482157078 2.0 2.0 24.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.694934014271677 1101 2 3.6736728143700113 1101 3 2.995571988531399 1101 4 3.0136149430090278 1101 5 2.440501457922011 1101 6 2.43566366674494 1101 7 2.9621439533170175 1101 8 2.8593907497980595 1101 9 3.1607602096593332 1101 10-11 2.9969561839637926 1101 12-13 2.9208840056114553 1101 14-15 2.9686924725485113 1101 16-17 2.6518657288693817 1101 18-19 2.789300236600411 1101 20-21 2.7311018489568006 1101 22-23 2.89775729574869 1101 24-25 3.23900185335685 1101 26-27 3.2916234088408274 1101 28-29 3.0991490345023074 1101 30-31 2.9461465460326544 1101 32-33 3.2231735567397095 1101 34-35 2.931647527425664 1101 36-37 2.971205421434565 1101 38-39 2.9604244701127875 1101 40-41 2.867749434589175 1101 42-43 2.6856836839912788 1101 44-45 2.4107349184211806 1101 46-47 2.530940043948089 1101 48-49 2.4356737107719297 1101 50-51 2.5024085433005077 1101 52-53 2.272972674578522 1101 54-55 2.398682771754796 1101 56-57 2.5942044627417147 1101 58-59 2.514086312516003 1101 60-61 2.2865123175964825 1101 62-63 2.4089896189108586 1101 64-65 2.43282686292606 1101 66-67 2.3936468098680734 1101 68-69 2.2120837321539213 1101 70-71 2.152421793557192 1101 72-73 1.857902232738386 1101 74-75 1.7135366582825746 1101 76-77 1.534998519429358 1101 78-79 1.5977990984057975 1101 80-81 1.5386203863662544 1101 82-83 1.6802521854529537 1101 84-85 1.453373551606827 1101 86-87 1.1719555366211232 1101 88-89 0.9854219485038822 1101 90-91 0.926741182234851 1101 92-93 0.7049743477544421 1101 94-95 0.622638276209118 1101 96-97 0.6937078142758608 1101 98-99 0.3575335946844884 1101 100-101 0.24382204741702118 1102 1 3.716995181871866 1102 2 3.559821169555537 1102 3 3.1215476761927263 1102 4 2.933554032420748 1102 5 2.613216554035823 1102 6 2.466657079578976 1102 7 2.937163290992366 1102 8 2.8970463933719586 1102 9 3.2679873105772863 1102 10-11 3.3591929320958265 1102 12-13 3.4456490497668426 1102 14-15 3.1686455802303186 1102 16-17 2.853403460195551 1102 18-19 2.9951581312527154 1102 20-21 3.3131756076695673 1102 22-23 3.2608514967937907 1102 24-25 3.446563224218867 1102 26-27 3.323789670219476 1102 28-29 3.035538971620877 1102 30-31 3.1096908422833742 1102 32-33 3.204958686746963 1102 34-35 3.58863686143118 1102 36-37 3.6042018282102894 1102 38-39 3.435535013038109 1102 40-41 3.5499248551063864 1102 42-43 3.54345284219076 1102 44-45 3.3412630068579965 1102 46-47 3.190400414687776 1102 48-49 3.135956578332948 1102 50-51 3.058106376986615 1102 52-53 2.877316809208285 1102 54-55 3.1175793874762654 1102 56-57 3.236454765781005 1102 58-59 2.87035950382554 1102 60-61 3.0214046815006412 1102 62-63 2.6953171873927957 1102 64-65 2.657207146034569 1102 66-67 2.639746530491177 1102 68-69 2.6139486594183126 1102 70-71 2.2723058045741062 1102 72-73 2.3244913015768027 1102 74-75 2.107431204316743 1102 76-77 2.2279263515956345 1102 78-79 2.1094987009022717 1102 80-81 1.9052535982367438 1102 82-83 1.806232506742564 1102 84-85 1.7979659838415571 1102 86-87 1.300364188037591 1102 88-89 1.2036112408685309 1102 90-91 1.1805479395476421 1102 92-93 0.9424105735838904 1102 94-95 0.8097693621709521 1102 96-97 0.5310626735927286 1102 98-99 0.3054967134746551 1102 100-101 -0.038152381750863995 1103 1 3.783106054681191 1103 2 3.7495083894508134 1103 3 3.052869602279923 1103 4 3.1260861551009107 1103 5 2.7314117364147847 1103 6 2.830514018005804 1103 7 3.109634808922671 1103 8 3.0836037996712946 1103 9 3.281611364078639 1103 10-11 3.248476620959398 1103 12-13 3.321703009501448 1103 14-15 3.1512096243357774 1103 16-17 3.094978766163024 1103 18-19 3.118896383375194 1103 20-21 3.0638555938040106 1103 22-23 3.324645685754689 1103 24-25 3.31804824785279 1103 26-27 3.295540620623065 1103 28-29 3.1821053833246644 1103 30-31 3.2150377035484787 1103 32-33 3.2786435011959796 1103 34-35 3.324661137574484 1103 36-37 3.3983835048856257 1103 38-39 3.4596557845831875 1103 40-41 3.1633389577259443 1103 42-43 3.1187560311492604 1103 44-45 3.0013261107727907 1103 46-47 3.1289386795463976 1103 48-49 3.238186241978987 1103 50-51 2.882035776177748 1103 52-53 2.63064187006535 1103 54-55 2.8291726742145897 1103 56-57 2.940399015779203 1103 58-59 3.0790496139810415 1103 60-61 3.0307940471933534 1103 62-63 3.038953718696039 1103 64-65 2.8060717681920657 1103 66-67 2.7903913570995975 1103 68-69 2.5805248064509136 1103 70-71 2.47644965734926 1103 72-73 2.4052686229766245 1103 74-75 2.3627747463784132 1103 76-77 2.267151225976548 1103 78-79 2.1523050108131443 1103 80-81 1.9166213443504212 1103 82-83 1.7036913486199161 1103 84-85 1.670107188463092 1103 86-87 1.3901769519217737 1103 88-89 1.5340208966566191 1103 90-91 1.3424246652330485 1103 92-93 1.4161814319989876 1103 94-95 1.0055029391728691 1103 96-97 0.8309263459473986 1103 98-99 0.6804492533711812 1103 100-101 0.501278478490546 1104 1 3.8425553715777987 1104 2 3.8411848970307148 1104 3 3.2861988552345984 1104 4 3.227047924353098 1104 5 2.7013141433581467 1104 6 2.562551351070308 1104 7 3.1674621753022763 1104 8 2.996730737851159 1104 9 3.5527333241965415 1104 10-11 3.536478147501061 1104 12-13 3.5359765940901013 1104 14-15 3.2865398829837815 1104 16-17 3.1603467323102876 1104 18-19 3.054491806630473 1104 20-21 3.3286850699794037 1104 22-23 3.2952669594088917 1104 24-25 3.274088468385262 1104 26-27 3.1492803476697304 1104 28-29 3.049141444271118 1104 30-31 3.2827149840000978 1104 32-33 3.6266865995955158 1104 34-35 3.648009844334478 1104 36-37 3.4986333080673653 1104 38-39 3.5243258859685973 1104 40-41 3.394396699884414 1104 42-43 3.257111770561874 1104 44-45 3.164469250456154 1104 46-47 3.2249367881708757 1104 48-49 3.3323169773112298 1104 50-51 3.0326932571919905 1104 52-53 2.819801624090527 1104 54-55 2.962543150994833 1104 56-57 3.1525286014047467 1104 58-59 3.121211626615814 1104 60-61 3.2387148422431338 1104 62-63 2.9234051957931158 1104 64-65 2.799711177929293 1104 66-67 2.786329580166697 1104 68-69 2.6763828020729115 1104 70-71 2.5556087033087245 1104 72-73 2.6558755793021724 1104 74-75 2.7889705973533836 1104 76-77 2.4631582154251674 1104 78-79 2.4975938536177544 1104 80-81 2.414927387151195 1104 82-83 2.410726161450544 1104 84-85 2.221690075354992 1104 86-87 1.986337975664778 1104 88-89 2.065423910856337 1104 90-91 1.7652152001946941 1104 92-93 1.7932555244747714 1104 94-95 1.3824747221543294 1104 96-97 1.0211689004564573 1104 98-99 0.6878635414171104 1104 100-101 0.5440357463650045 1105 1 3.8120073077813075 1105 2 3.3691444445587386 1105 3 2.1020956112272486 1105 4 2.6243925119733866 1105 5 2.5218790976659875 1105 6 2.5874029537013534 1105 7 3.0976541156830066 1105 8 3.154091126272384 1105 9 3.591384595676697 1105 10-11 3.6366929112609263 1105 12-13 3.684987606219181 1105 14-15 3.626003518439326 1105 16-17 3.4842872916344163 1105 18-19 3.0333941050113467 1105 20-21 3.3817829501899546 1105 22-23 3.5090667796066697 1105 24-25 3.462287888417169 1105 26-27 3.4610602685446494 1105 28-29 3.420074554213823 1105 30-31 3.6417979472859408 1105 32-33 3.8981849211387782 1105 34-35 4.19827387208576 1105 36-37 4.21759184761326 1105 38-39 4.152218840082707 1105 40-41 4.007312492512529 1105 42-43 3.890402045199167 1105 44-45 3.7269090515234673 1105 46-47 3.9233854643240775 1105 48-49 3.9997489385175378 1105 50-51 3.7561605492313497 1105 52-53 3.450845884494992 1105 54-55 3.6704841129064256 1105 56-57 3.930524548287419 1105 58-59 3.7850206034080074 1105 60-61 3.8438073465490064 1105 62-63 3.8001881587762583 1105 64-65 3.746270947031764 1105 66-67 3.77860156583095 1105 68-69 3.8083539973944163 1105 70-71 3.657658569667852 1105 72-73 3.5352692311336433 1105 74-75 3.4254157349747274 1105 76-77 3.4610126967078827 1105 78-79 3.589392799020235 1105 80-81 3.5936144677262014 1105 82-83 3.433085505565959 1105 84-85 3.4535096193111876 1105 86-87 3.4569944959167422 1105 88-89 3.5027578435303948 1105 90-91 3.379465358354052 1105 92-93 3.1820379395757374 1105 94-95 2.8965155651058705 1105 96-97 2.9022771813474755 1105 98-99 2.52046094185239 1105 100-101 2.2845853841876416 1106 1 3.273854096787044 1106 2 2.438325076192438 1106 3 2.743415117753262 1106 4 1.3635355966734117 1106 5 1.7827522670497942 1106 6 2.05767748517739 1106 7 2.6892458781133186 1106 8 2.536154744756523 1106 9 2.7385376928923755 1106 10-11 2.703115224867222 1106 12-13 2.940813069866927 1106 14-15 2.7603134513099548 1106 16-17 1.5506348364550604 1106 18-19 2.5536247504610188 1106 20-21 1.966695096866804 1106 22-23 2.7907451763038438 1106 24-25 2.9010506275553745 1106 26-27 2.934118311878038 1106 28-29 2.2597450839344155 1106 30-31 1.6052002521150293 1106 32-33 1.8658799295096387 1106 34-35 2.364703500029787 1106 36-37 2.5325626715327694 1106 38-39 1.1248290834063432 1106 40-41 1.3980980523844444 1106 42-43 2.6609463944669223 1106 44-45 2.8529749130711757 1106 46-47 2.8012831611203985 1106 48-49 2.965319538130693 1106 50-51 2.868440509571389 1106 52-53 2.6026704054058705 1106 54-55 2.2452569620847846 1106 56-57 2.5179682685221287 1106 58-59 2.854644158826307 1106 60-61 2.8643395052538807 1106 62-63 2.43653716646444 1106 64-65 2.6684268887121085 1106 66-67 2.6623856790894074 1106 68-69 3.0016248066852533 1106 70-71 2.785858592423569 1106 72-73 2.862329842612276 1106 74-75 2.6554264626700608 1106 76-77 2.4942774919876953 1106 78-79 2.44323038821452 1106 80-81 2.2755366756809465 1106 82-83 2.4219156991520983 1106 84-85 2.3080908328410885 1106 86-87 2.140978534406411 1106 88-89 2.188148672976295 1106 90-91 2.144530455959826 1106 92-93 2.2037907799768863 1106 94-95 2.1743311787788056 1106 96-97 2.114873283618172 1106 98-99 1.8913669444855525 1106 100-101 1.7011824291951996 1107 1 2.9313576791775056 1107 2 3.25962624862483 1107 3 -0.24890221800298562 1107 4 2.007312483436724 1107 5 1.0601338598160055 1107 6 1.8656178103122834 1107 7 2.679228896603121 1107 8 1.9452676529644535 1107 9 -1.1430047465686144 1107 10-11 -0.4442966392867227 1107 12-13 2.1451507382317416 1107 14-15 1.886780875288693 1107 16-17 -0.37491291895695156 1107 18-19 0.9492247010383394 1107 20-21 1.1613597836129408 1107 22-23 1.223456808562986 1107 24-25 0.43061563268598846 1107 26-27 1.0554563252974987 1107 28-29 1.5976267347119908 1107 30-31 2.059790156931175 1107 32-33 0.1934717946580662 1107 34-35 1.4408877230465862 1107 36-37 2.5363512660453473 1107 38-39 0.5628735427177709 1107 40-41 1.2649859156523995 1107 42-43 0.9283943311456255 1107 44-45 1.294836595883087 1107 46-47 1.4211105433912188 1107 48-49 1.1616437267625699 1107 50-51 1.899422197566647 1107 52-53 1.9680674517983903 1107 54-55 1.5249307573694395 1107 56-57 1.6717966422600021 1107 58-59 1.6460722631503408 1107 60-61 2.1256774131981224 1107 62-63 2.3010537961394384 1107 64-65 2.507920426848184 1107 66-67 1.6789979506790509 1107 68-69 0.9275620670116531 1107 70-71 0.9021940669038422 1107 72-73 1.4084718604737496 1107 74-75 1.1920118230110752 1107 76-77 1.410168614439904 1107 78-79 2.002860727709116 1107 80-81 2.2039292484174133 1107 82-83 2.251788842599904 1107 84-85 2.508722328042719 1107 86-87 2.1403665521202235 1107 88-89 2.6238246109146726 1107 90-91 2.401274918134151 1107 92-93 2.3954448272870383 1107 94-95 2.2269254171196504 1107 96-97 2.123461452613581 1107 98-99 2.0640854024981277 1107 100-101 1.9926416487087177 1108 1 3.0073566492994424 1108 2 3.188812235169923 1108 3 2.6189086029845683 1108 4 2.6712594060574766 1108 5 2.1674626174598686 1108 6 2.1063785592779602 1108 7 1.9785473369100117 1108 8 2.24180186586932 1108 9 2.6200555685647906 1108 10-11 -0.410084739556023 1108 12-13 1.9062848257265301 1108 14-15 1.6036154383675694 1108 16-17 1.6574549423527998 1108 18-19 0.22790166339289897 1108 20-21 -0.10290754551582637 1108 22-23 0.9694060708316385 1108 24-25 -0.20498637047323243 1108 26-27 0.8368030841583547 1108 28-29 0.431522789706861 1108 30-31 0.3529288649599742 1108 32-33 1.7082419625235445 1108 34-35 -0.31290693689649274 1108 36-37 1.1863482119775206 1108 38-39 1.9680191444348516 1108 40-41 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-4.153655963565491 2312 64-65 -1.9929841133035104 2312 66-67 1.2111919102855246 2312 68-69 -0.514025989654332 2312 70-71 -1.2813506705080577 2312 72-73 -1.5739522468085667 2312 74-75 -1.147821876037849 2312 76-77 -1.6014763301210593 2312 78-79 0.30425313999931447 2312 80-81 0.723418603885424 2312 82-83 -0.4934096187015733 2312 84-85 -0.8216403605647287 2312 86-87 0.11294768550461498 2312 88-89 -1.9510727571180109 2312 90-91 -1.9007686725789323 2312 92-93 -1.4066699675513608 2312 94-95 -1.1739914033199188 2312 96-97 -0.9291984685124994 2312 98-99 -0.12350019595584882 2312 100-101 0.1619929586599529 2313 1 -2.1985743522419874 2313 2 -0.9965351561978579 2313 3 -2.5033993287686904 2313 4 -1.8544516484506524 2313 5 -1.8409472378651799 2313 6 -3.3936201609528887 2313 7 0.4692411509587693 2313 8 -3.031426682590908 2313 9 -3.8819667620287177 2313 10-11 -5.2259816775803465 2313 12-13 -3.695484090885877 2313 14-15 -3.439903702352108 2313 16-17 -1.9318662503684223 2313 18-19 -4.565475275285095 2313 20-21 -6.679442564626719 2313 22-23 -5.500130993350361 2313 24-25 -1.9846007701207249 2313 26-27 -5.362087513495808 2313 28-29 -6.114146461634089 2313 30-31 -5.634128829592992 2313 32-33 -5.070001705917008 2313 34-35 -0.020626071276385716 2313 36-37 0.20921158323205447 2313 38-39 -2.551339726659119 2313 40-41 -6.070979181723661 2313 42-43 -3.0365853555510185 2313 44-45 -1.6943504460026375 2313 46-47 -3.405871724942518 2313 48-49 -3.0919381213369164 2313 50-51 -2.5585460560242126 2313 52-53 -4.3013282608612435 2313 54-55 -4.014897978846886 2313 56-57 -3.231553740180903 2313 58-59 -0.9178625836999359 2313 60-61 0.8093736716075135 2313 62-63 0.32037367444784337 2313 64-65 1.146985866296113 2313 66-67 0.1955063608126224 2313 68-69 -2.5845107022646836 2313 70-71 -0.8959817479315539 2313 72-73 -2.290147270784562 2313 74-75 -2.6768070334405856 2313 76-77 -1.6226488868008886 2313 78-79 -0.9996290760131572 2313 80-81 -1.2493800715302434 2313 82-83 -1.1473434459926501 2313 84-85 -1.3778741608912135 2313 86-87 -1.489546855056254 2313 88-89 -2.0993805024306837 2313 90-91 -3.541141200950049 2313 92-93 -3.9463301423247312 2313 94-95 -3.2435060213950866 2313 96-97 -2.871644829403028 2313 98-99 -2.4338707849608223 2313 100-101 -2.346865720250414 2314 1 -2.328574935603662 2314 2 -0.48485525955247155 2314 3 -2.6980084977913315 2314 4 -1.5801882848441657 2314 5 -2.6137563235097865 2314 6 -2.3201865937531814 2314 7 -1.185864330791258 2314 8 0.443027625250469 2314 9 -1.7599482737322347 2314 10-11 -2.1339357428286405 2314 12-13 -1.2720537250611947 2314 14-15 -3.6604792953534755 2314 16-17 -1.7526473005047976 2314 18-19 -1.0118516744638342 2314 20-21 -1.412422561012722 2314 22-23 -1.9479581134076156 2314 24-25 -2.3775661397799404 2314 26-27 -1.901307060949435 2314 28-29 -1.454568872397541 2314 30-31 -1.6058461752120188 2314 32-33 -8.350848234843923 2314 34-35 -8.859953754064232 2314 36-37 -5.0505644912561145 2314 38-39 -3.362705461701939 2314 40-41 -2.7219141966563 2314 42-43 -1.7169683253741432 2314 44-45 -1.2068474758380283 2314 46-47 -2.2453270358814024 2314 48-49 -1.7490250408031258 2314 50-51 -1.2569500638611792 2314 52-53 -0.8160836117817425 2314 54-55 0.4348267740425307 2314 56-57 -0.05409526201498949 2314 58-59 -2.808825457709464 2314 60-61 -3.4089030495584147 2314 62-63 -2.9006118368402056 2314 64-65 -3.2794993227201843 2314 66-67 -2.97461129327667 2314 68-69 -4.28753479225605 2314 70-71 -6.377231650111764 2314 72-73 -6.388512008421209 2314 74-75 -6.408541339233086 2314 76-77 -7.401645590096027 2314 78-79 -6.481306539613524 2314 80-81 -5.499231598870697 2314 82-83 -4.643927223131417 2314 84-85 -4.556624571734339 2314 86-87 -4.2842463739963 2314 88-89 -4.570426412994225 2314 90-91 -5.138942212015424 2314 92-93 -5.102847074752953 2314 94-95 -4.428266764853966 2314 96-97 -4.436014663628715 2314 98-99 -4.710506011753344 2314 100-101 -4.354660428164071 2315 1 -24.639056522005927 2315 2 -12.815505243814354 2315 3 -7.987061921547305 2315 4 -5.448412667480731 2315 5 -2.208319161521633 2315 6 -1.8509943168767684 2315 7 -7.540737782017402 2315 8 -1.7628012012582452 2315 9 -5.221843429253454 2315 10-11 -3.0401419264781673 2315 12-13 -3.537833691868073 2315 14-15 -3.2692468576621145 2315 16-17 -5.757191324312281 2315 18-19 -6.773573174163381 2315 20-21 -5.4459316962746875 2315 22-23 -7.5276541531248995 2315 24-25 -23.711840362173852 2315 26-27 -10.51345617246115 2315 28-29 -6.897565946538382 2315 30-31 -5.326013142333661 2315 32-33 -5.853440374369491 2315 34-35 -6.780127159506758 2315 36-37 -5.42351066689351 2315 38-39 -4.033959431999818 2315 40-41 -4.09295480815371 2315 42-43 -3.170718468447941 2315 44-45 -8.030704316196285 2315 46-47 -4.640922509026449 2315 48-49 -4.265741067929916 2315 50-51 -5.8119903749036155 2315 52-53 -9.320065355565188 2315 54-55 -5.444569347226821 2315 56-57 -11.722551424203449 2315 58-59 -7.150159656639271 2315 60-61 -4.642207052763268 2315 62-63 -4.682979958567394 2315 64-65 -4.564143033196046 2315 66-67 -7.484437065159808 2315 68-69 -4.953841091795351 2315 70-71 -4.944533564439176 2315 72-73 -5.163755591561394 2315 74-75 -4.500046332508617 2315 76-77 -5.465005463618791 2315 78-79 -4.777938761263449 2315 80-81 -4.412652994126368 2315 82-83 -3.9801989345801836 2315 84-85 -3.8750297638824502 2315 86-87 -3.6745268344077804 2315 88-89 -4.869430798206119 2315 90-91 -4.4319990194667085 2315 92-93 -4.530368035064283 2315 94-95 -4.687814437043281 2315 96-97 -4.804682038510679 2315 98-99 -4.634821940159215 2315 100-101 -4.1732737926874925 2316 1 -2.108960762772 2316 2 -0.3824417083226379 2316 3 -4.398429357880541 2316 4 -0.46450640236721696 2316 5 -4.6600586839690905 2316 6 -0.9139589495233409 2316 7 -3.566381531412862 2316 8 -3.7414315540336034 2316 9 -2.7287282832655286 2316 10-11 -2.285208011404034 2316 12-13 -4.52996170938939 2316 14-15 -3.8272689389400085 2316 16-17 -1.773085560794751 2316 18-19 -6.109299192900686 2316 20-21 -2.7856900009355243 2316 22-23 -5.696130123498953 2316 24-25 -2.4362652713200355 2316 26-27 -2.3979388238726678 2316 28-29 -5.372190354088577 2316 30-31 -9.20949052906002 2316 32-33 -6.0731468439904575 2316 34-35 -3.0575698490561507 2316 36-37 -4.586301364567927 2316 38-39 -4.809876088302932 2316 40-41 -3.148275888312341 2316 42-43 -2.6781461135722324 2316 44-45 -0.13961662671598063 2316 46-47 -1.7763102441063872 2316 48-49 -1.9270445956835118 2316 50-51 -0.1860969502328338 2316 52-53 1.1649627642098572 2316 54-55 1.542323780355435 2316 56-57 -0.632750369884608 2316 58-59 -3.161611815022006 2316 60-61 -5.946731434750031 2316 62-63 -2.2459294776985104 2316 64-65 -3.8855820298658905 2316 66-67 -2.906173668539708 2316 68-69 0.26144057231497086 2316 70-71 0.8935255492697358 2316 72-73 1.370804268323365 2316 74-75 1.3966411303553166 2316 76-77 0.8755244939597304 2316 78-79 0.38216388973352267 2316 80-81 1.1725155517979893 2316 82-83 0.7438550457152466 2316 84-85 0.49203650413286226 2316 86-87 0.006529194416717843 2316 88-89 -1.7647800672301468 2316 90-91 -1.8976062718814166 2316 92-93 -2.218060624432658 2316 94-95 -3.4884239867123394 2316 96-97 -4.721505453923356 2316 98-99 -3.0293429885362837 2316 100-101 -2.2891750245685465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36511.0 3 12058.0 4 12418.0 5 14149.0 6 15915.0 7 20179.0 8 25382.0 9 29772.0 10 34097.0 11 38100.0 12 42115.0 13 46179.0 14 49842.0 15 53520.0 16 56843.0 17 58049.0 18 53696.0 19 53033.0 20 56048.0 21 59525.0 22 64257.0 23 69178.0 24 74209.0 25 79221.0 26 84574.0 27 90004.0 28 96792.0 29 103415.0 30 109148.0 31 115586.0 32 122882.0 33 128287.0 34 131948.0 35 126458.0 36 107893.0 37 60476.0 38 8846.0 39 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.480486886963384 19.577945209296033 15.091567704733237 20.85000019900735 2 16.09038820655355 20.79831021283491 40.228471904322134 22.88282967628941 3 19.151599467693284 26.73675429347164 29.950197715157184 24.16144852367789 4 12.50208603835551 17.676711704971435 37.03018858649179 32.79101367018127 5 12.96434719335347 38.26706112679933 33.470221178609954 15.298370501237251 6 30.110081893057806 36.810255774112896 17.772825363963204 15.306836968866094 7 27.75356197017202 34.44053078344865 20.62409969733743 17.181807549041903 8 27.242381243723884 33.66338853511973 19.443377316261007 19.65085290489538 9 27.887100141700266 16.773298398855445 20.489935516110705 34.849665943333584 10-11 25.178313859142946 27.208768412246197 26.206344871149334 21.406572857461526 12-13 26.79467622549479 25.218408583476176 27.166398858385094 20.820516332643948 14-15 23.755458928349537 25.912222023005643 26.654038628810934 23.67828041983389 16-17 21.4076676667041 29.306102367468302 27.476258469568382 21.809971496259216 18-19 21.17248679784247 26.48503413178111 31.636977679346955 20.705501391029465 20-21 22.246451144094397 25.51521586278998 32.01238991603256 20.225943077083063 22-23 21.85975358178011 25.65865816047613 31.800805062455517 20.680783195288242 24-25 21.3854954724776 26.21338033353398 32.017984505523536 20.383139688464887 26-27 21.12491813520179 26.32998851805393 31.9113532960336 20.63374005071068 28-29 20.754308946605825 26.176032970611786 32.3978270537419 20.67183102904049 30-31 20.88690621707331 26.25082363559065 32.14891074594519 20.713359401390857 32-33 20.78459685268854 26.47425579046129 31.75967760376454 20.981469753085626 34-35 21.518902930895404 26.45291745092767 31.473535896746434 20.554643721430494 36-37 21.705215773162347 26.075373747302223 31.361416672701008 20.857993806834422 38-39 21.245467071573998 26.364548558031764 31.476667843457157 20.913316526937077 40-41 21.245963620537065 26.220809340668282 31.849486419173456 20.683740619621194 42-43 21.404267825794342 26.419403030123352 31.05250302950257 21.123826114579735 44-45 21.150284049813358 26.823362701267932 30.924559245592455 21.101794003326255 46-47 21.636588288634265 27.050567895018734 30.066411757175803 21.246432059171198 48-49 21.815707381531364 27.30630137645814 29.48283844144411 21.395152800566386 50-51 21.747854800743198 27.464460451311425 29.32993210607358 21.457752641871796 52-53 21.789940853748384 27.584136056939407 29.278251244778442 21.34767184453377 54-55 21.312077378724577 27.577867933174556 29.42392072571262 21.686133962388247 56-57 21.39885849235534 28.103021685733893 28.981330086317364 21.5167897355934 58-59 21.514173612438455 27.41354020428392 29.153855489276925 21.918430694000698 60-61 21.412321597337314 27.752507396348395 29.353166219233245 21.482004787081046 62-63 21.521326874262535 27.646059868379098 29.39825187380246 21.434361383555906 64-65 21.29729031114999 27.530753694198644 29.356984649739875 21.814971344911488 66-67 21.142299365581852 27.88565663690754 29.04675912569971 21.925284871810895 68-69 21.31867195796865 27.663611369250663 29.27249020936485 21.745226463415833 70-71 21.131243055720546 27.845546596529847 28.977543075134026 22.04566727261558 72-73 21.303443337923195 27.804575168018246 28.694596922201754 22.19738457185681 74-75 21.1436825071106 28.243148687547265 28.570737301337847 22.04243150400429 76-77 21.398421162950875 27.958595939368646 28.7352539355846 21.90772896209588 78-79 21.31962242184474 27.978300667083854 28.560818869573428 22.14125804149797 80-81 21.27854505030712 28.579479080666104 28.21384464915195 21.92813121987482 82-83 21.502053339283428 28.363534201835822 27.986335625575755 22.148076833304998 84-85 21.25091463036089 28.161345719358653 27.88364917069946 22.704090479580998 86-87 21.459253741566492 27.99483715195726 28.17271139422583 22.37319771225042 88-89 21.427475182408788 28.026127903519576 27.948341822946983 22.59805509112466 90-91 21.27349882810928 28.30781891312595 28.05490117497352 22.363781083791256 92-93 21.428424644338744 27.95433236720767 28.04682783983149 22.570415148622093 94-95 21.119122869778128 27.71008066492958 28.11065432597503 23.060142139317254 96-97 21.522486637096474 27.458951978343553 27.816552313074595 23.202009071485378 98-99 21.283605148486608 27.612776001084505 27.591531351598736 23.51208749883015 100-101 21.453731759678263 27.438633601939777 27.901914900318776 23.205719738063184 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6397.0 1 4293.0 2 2238.5 3 2322.0 4 2100.0 5 1994.5 6 2734.0 7 4564.5 8 6799.5 9 7931.0 10 7229.0 11 6043.0 12 5540.5 13 5384.5 14 5459.5 15 5544.0 16 5654.0 17 5914.5 18 6250.5 19 6811.0 20 7449.5 21 8355.0 22 9536.0 23 10873.5 24 12545.5 25 14401.0 26 17065.0 27 20088.5 28 22999.0 29 26409.0 30 30297.5 31 35071.0 32 41289.0 33 46934.0 34 51758.0 35 56865.5 36 62749.0 37 68282.0 38 70967.0 39 71682.0 40 71258.5 41 70998.0 42 71779.0 43 73028.0 44 74019.0 45 75635.5 46 75993.5 47 75465.0 48 77582.5 49 77242.0 50 73540.0 51 70452.5 52 68537.5 53 67922.0 54 66860.0 55 63523.5 56 59047.0 57 54442.5 58 49946.0 59 44729.0 60 38521.0 61 32937.5 62 27929.0 63 22482.5 64 17587.0 65 13930.0 66 10635.0 67 8101.5 68 6017.5 69 4183.0 70 3045.0 71 2347.5 72 1833.0 73 1397.5 74 1099.5 75 897.5 76 650.0 77 428.5 78 305.0 79 232.0 80 151.0 81 88.5 82 60.0 83 45.5 84 33.5 85 25.0 86 16.0 87 12.0 88 7.5 89 4.0 90 2.5 91 4.5 92 5.0 93 4.0 94 2.5 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.3932910886216123 2 3.4991164839822133 3 1.0548360966706656 4 0.9253420420328047 5 0.9486689156165301 6 0.12146209083980164 7 1.7399882425721425 8 0.9116706142914269 9 0.14184106281679262 10-11 0.9811385565023019 12-13 0.8127877408307443 14-15 0.6353368810688322 16-17 0.1382309514288351 18-19 0.15081293727207168 20-21 0.16700503450326576 22-23 0.592656392588719 24-25 1.3431323291669657 26-27 0.22397643729428776 28-29 0.23401639204186192 30-31 0.21220619247319544 32-33 0.23006449496036996 34-35 0.6952348238607429 36-37 0.28987699132889694 38-39 0.2826567685529819 40-41 0.2671909659205485 42-43 0.20799795612155267 44-45 0.17456704297271525 46-47 0.2100700318886052 48-49 0.6115827753681887 50-51 0.23961313277348836 52-53 0.2604406984732433 54-55 0.2895138440295166 56-57 0.21212074604981182 58-59 0.19800072458567028 60-61 0.1862732029762698 62-63 0.17469521260779067 64-65 0.16993157450415441 66-67 0.18516239947228288 68-69 0.19169905086112907 70-71 0.19834251027920474 72-73 0.2629400063572139 74-75 0.23079078955913065 76-77 0.22735157101794035 78-79 0.22149849101616306 80-81 0.23036355744221257 82-83 0.24228333350422618 84-85 0.21382967451748403 86-87 0.20199534487885407 88-89 0.20312750998868692 90-91 0.21814471889835635 92-93 0.20754936239878874 94-95 0.21884965189127112 96-97 0.2409161907300884 98-99 0.25388268547855125 100-101 0.30694491439977306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2340648.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.19051081054672 #Duplication Level Percentage of deduplicated Percentage of total 1 78.25710664020929 42.407925833883695 2 11.361869642051536 12.31411039331233 3 4.171445450965277 6.781582794184193 4 2.0391182132701267 4.420034303207901 5 1.1003400750052916 2.9813995364926025 6 0.715924917801839 2.32778021986082 7 0.46142991592306154 1.7503585992996833 8 0.31425494737734033 1.3623708898495643 9 0.2318248846599264 1.1306438026485741 >10 1.1850613030706374 11.707038971343575 >50 0.10271655855522523 3.861235564798269 >100 0.055040589749583896 5.4253632648035515 >500 0.0028122628080295775 1.0702146607616398 >1k 7.030657020073908E-4 0.6512155247087312 >5k 1.757664255018477E-4 0.6030196704594087 >10k+ 1.757664255018477E-4 1.2057059703854724 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13822 0.5905202320041287 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11495 0.4911033183972985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7519 0.3212358287106818 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5143 0.21972547773095313 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2814 0.12022311770073929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 8.544642338361001E-5 0.0 0.0 0.0 0.0 6 8.544642338361001E-5 0.0 0.0 0.0 0.0 7 8.544642338361001E-5 0.0 0.0 0.0 0.0 8 8.544642338361001E-5 0.0 0.0 0.0 0.0 9 8.544642338361001E-5 0.0 0.0 0.0 0.0 10-11 8.544642338361001E-5 0.0 0.0 0.0 2.1361605845902503E-5 12-13 1.2816963507541503E-4 0.0 0.0 2.1361605845902503E-5 4.2723211691805006E-5 14-15 1.2816963507541503E-4 0.0 0.0 4.2723211691805006E-5 4.2723211691805006E-5 16-17 1.2816963507541503E-4 0.0 0.0 6.408481753770751E-5 4.2723211691805006E-5 18-19 1.2816963507541503E-4 0.0 0.0 2.3497766430492754E-4 4.2723211691805006E-5 20-21 1.2816963507541503E-4 0.0 0.0 3.845089052262451E-4 4.2723211691805006E-5 22-23 1.2816963507541503E-4 0.0 0.0 8.117410221442951E-4 4.2723211691805006E-5 24-25 1.2816963507541503E-4 4.2723211691805006E-5 0.0 0.0013244195624459551 4.2723211691805006E-5 26-27 1.2816963507541503E-4 8.544642338361001E-5 0.0 0.0020293525553607378 4.2723211691805006E-5 28-29 1.2816963507541503E-4 8.544642338361001E-5 0.0 0.003610111387957523 4.2723211691805006E-5 30-31 1.2816963507541503E-4 8.544642338361001E-5 0.0 0.008480557520823294 4.2723211691805006E-5 32-33 1.2816963507541503E-4 8.544642338361001E-5 4.2723211691805006E-5 0.015850311537659655 4.2723211691805006E-5 34-35 1.2816963507541503E-4 8.544642338361001E-5 4.2723211691805006E-5 0.026894261759991253 4.2723211691805006E-5 36-37 1.2816963507541503E-4 8.544642338361001E-5 4.2723211691805006E-5 0.04710234089021502 4.2723211691805006E-5 38-39 1.2816963507541503E-4 8.544642338361001E-5 4.2723211691805006E-5 0.07726492834462936 4.2723211691805006E-5 40-41 1.2816963507541503E-4 8.544642338361001E-5 4.2723211691805006E-5 0.10883738178487326 4.2723211691805006E-5 42-43 1.7089284676722002E-4 8.544642338361001E-5 4.2723211691805006E-5 0.14043119683096306 4.2723211691805006E-5 44-45 1.7089284676722002E-4 8.544642338361001E-5 4.2723211691805006E-5 0.17967246676988596 4.2723211691805006E-5 46-47 2.1361605845902502E-4 8.544642338361001E-5 4.2723211691805006E-5 0.23002177174867816 4.2723211691805006E-5 48-49 2.1361605845902502E-4 8.544642338361001E-5 4.2723211691805006E-5 0.2878262771676903 4.2723211691805006E-5 50-51 2.1361605845902502E-4 8.544642338361001E-5 4.2723211691805006E-5 0.3495185948506567 4.2723211691805006E-5 52-53 2.1361605845902502E-4 8.544642338361001E-5 4.2723211691805006E-5 0.40933109121918376 8.544642338361001E-5 54-55 2.1361605845902502E-4 1.0680802922951251E-4 4.2723211691805006E-5 0.4743130962024192 8.544642338361001E-5 56-57 2.1361605845902502E-4 1.7089284676722002E-4 4.2723211691805006E-5 0.5523897655691927 1.0680802922951251E-4 58-59 2.1361605845902502E-4 1.7089284676722002E-4 4.2723211691805006E-5 0.643283398443508 1.2816963507541503E-4 60-61 2.1361605845902502E-4 1.7089284676722002E-4 4.2723211691805006E-5 0.7451141735109252 1.2816963507541503E-4 62-63 2.1361605845902502E-4 1.7089284676722002E-4 4.2723211691805006E-5 0.8470517566075719 1.2816963507541503E-4 64-65 2.1361605845902502E-4 1.7089284676722002E-4 4.2723211691805006E-5 0.9499506119672843 1.2816963507541503E-4 66-67 2.5633927015083005E-4 1.7089284676722002E-4 4.2723211691805006E-5 1.0715622340480073 1.2816963507541503E-4 68-69 2.5633927015083005E-4 1.7089284676722002E-4 4.2723211691805006E-5 1.2099427167177637 1.2816963507541503E-4 70-71 2.5633927015083005E-4 1.7089284676722002E-4 4.2723211691805006E-5 1.3684458320943602 1.2816963507541503E-4 72-73 2.5633927015083005E-4 1.7089284676722002E-4 4.2723211691805006E-5 1.529298724114006 1.2816963507541503E-4 74-75 2.7770087599673254E-4 1.7089284676722002E-4 4.2723211691805006E-5 1.6916042053311733 1.2816963507541503E-4 76-77 2.99062481842635E-4 1.7089284676722002E-4 4.2723211691805006E-5 1.8678801767715607 1.2816963507541503E-4 78-79 2.99062481842635E-4 1.7089284676722002E-4 4.2723211691805006E-5 2.0652400531818538 1.2816963507541503E-4 80-81 3.4178569353444005E-4 1.7089284676722002E-4 4.2723211691805006E-5 2.2932111107693256 1.2816963507541503E-4 82-83 3.4178569353444005E-4 1.7089284676722002E-4 4.2723211691805006E-5 2.524642748503833 1.7089284676722002E-4 84-85 3.6314729938034254E-4 1.7089284676722002E-4 4.2723211691805006E-5 2.7595990512029145 1.7089284676722002E-4 86-87 3.84508905226245E-4 1.7089284676722002E-4 4.2723211691805006E-5 2.9981441036841083 1.7089284676722002E-4 88-89 3.84508905226245E-4 1.7089284676722002E-4 4.2723211691805006E-5 3.2521335971918885 1.7089284676722002E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9190 0.0 47.945744 1 GTATCAA 15685 0.0 40.190567 1 TATCAAC 20085 0.0 31.173765 2 ATCAACG 20875 0.0 29.909151 3 TCAACGC 21355 0.0 29.32481 4 CAACGCA 21440 0.0 28.99314 5 AACGCAG 21930 0.0 28.330412 6 GTACATG 13970 0.0 27.347088 1 TACATGG 14545 0.0 25.868729 2 ACGCAGA 24140 0.0 25.066418 7 CGCAGAG 24330 0.0 25.002012 8 GAGTACT 13385 0.0 24.751867 12-13 ACATGGG 14540 0.0 23.991264 3 GCAGAGT 24035 0.0 23.788078 9 GTACTTT 14705 0.0 22.65858 14-15 AGTACTT 14225 0.0 22.178782 12-13 CATGGGA 8690 0.0 22.092842 4 ATGGGAT 3715 0.0 21.191917 5 ACTTTTT 17375 0.0 20.733006 16-17 CAGAGTA 23665 0.0 20.717924 10-11 >>END_MODULE