FastQCFastQC Report
Fri 27 May 2016
SRR939547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2340648
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199800.853609769602264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183520.7840563809680055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126660.5411321992884022No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80340.34323828273196144No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47380.2024225769957721No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36630.15649512442708174No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26000.11108035039869302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107200.046.5536731
GTATCAA178600.036.619971
TATCAAC226600.028.6672842
ATCAACG229400.028.2104913
TCAACGC231150.028.0174064
CAACGCA234450.027.602845
AACGCAG240600.026.9760446
ACGCAGA272100.023.7486637
CGCAGAG273500.023.6097768
GAGTACT161800.023.43984212-13
AGGTAAG9350.023.3106962
GTACATG150800.023.0682741
GGTAAGG10000.022.7402153
GCAGAGT281550.022.2953179
TAAGGTG12550.022.2721235
TACATGG155650.022.0697732
GTACTTT173050.021.84757414-15
TGGTATC57850.021.4588742
ACATGGG148650.021.3532123
AGTACTT169550.021.05510112-13
Produced by FastQC (version 0.11.5)