##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939547_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2340648 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.969346950075362 31.0 31.0 31.0 30.0 34.0 2 31.218321165762642 31.0 31.0 34.0 30.0 34.0 3 31.181326282294474 31.0 31.0 34.0 28.0 34.0 4 35.14119765124872 35.0 35.0 37.0 33.0 37.0 5 34.76684490790584 35.0 35.0 37.0 32.0 37.0 6 34.74164291256096 35.0 35.0 37.0 32.0 37.0 7 34.60927059515143 35.0 35.0 37.0 30.0 37.0 8 34.62561948656953 35.0 35.0 37.0 30.0 37.0 9 35.9466857041298 37.0 35.0 39.0 30.0 39.0 10-11 35.81000047849997 37.0 35.0 39.0 30.0 39.0 12-13 35.76516182698124 37.0 35.0 39.0 30.0 39.0 14-15 36.56595417166528 38.0 35.0 40.0 30.0 41.0 16-17 36.581579759109445 38.0 35.0 40.0 30.5 41.0 18-19 36.60600226945701 38.0 35.0 40.0 30.0 41.0 20-21 36.53816165437947 38.0 35.0 40.0 30.0 41.0 22-23 36.394395270027786 38.0 35.0 40.0 30.0 41.0 24-25 36.16033423222971 38.0 34.0 40.0 29.5 41.0 26-27 35.76046825494478 38.0 34.0 40.0 27.5 41.0 28-29 35.470722423875785 38.0 34.0 40.0 27.0 41.0 30-31 35.19074867301705 37.5 34.0 40.0 27.0 41.0 32-33 34.89785050977336 37.0 33.0 40.0 27.0 41.0 34-35 34.76994639945861 37.0 33.0 40.0 25.5 41.0 36-37 34.67762346153715 37.0 33.0 40.0 25.0 41.0 38-39 34.608268522221195 37.0 33.0 40.0 25.0 41.0 40-41 34.34130847525984 37.0 33.0 40.0 24.5 41.0 42-43 33.971364553747506 37.0 33.0 40.0 23.5 41.0 44-45 33.698868433015136 37.0 33.0 40.0 23.0 41.0 46-47 33.24886698042593 37.0 32.0 40.0 21.0 41.0 48-49 32.82368792744573 37.0 31.0 40.0 18.5 41.0 50-51 32.51152522720204 36.0 31.0 39.5 15.5 41.0 52-53 32.12233706221525 36.0 31.0 39.0 13.5 40.5 54-55 31.883829392544285 36.0 30.0 39.0 11.5 40.0 56-57 31.49163522238286 35.0 30.0 39.0 8.5 40.0 58-59 31.052744581842294 35.0 29.0 39.0 8.0 40.0 60-61 30.755360054138855 35.0 28.5 38.0 7.0 40.0 62-63 30.364327741719386 34.5 28.0 38.0 4.0 40.0 64-65 29.931135095922155 34.0 28.0 38.0 2.0 40.0 66-67 28.278895416995635 33.0 24.5 36.5 2.0 39.5 68-69 24.73075874715036 29.5 13.0 35.0 2.0 38.0 70-71 24.127161580895546 28.5 10.5 34.5 2.0 38.0 72-73 25.888318106780687 31.0 20.0 35.0 2.0 37.5 74-75 26.469136111025666 31.0 22.0 35.0 2.0 37.0 76-77 26.500840151957917 31.5 23.5 34.5 2.0 36.0 78-79 27.30050802171023 32.5 26.0 35.0 2.0 37.0 80-81 27.15659659205485 33.0 25.5 35.0 2.0 36.0 82-83 26.75255335274676 32.0 25.0 35.0 2.0 36.0 84-85 26.412993324925406 32.0 24.5 35.0 2.0 36.0 86-87 25.97191504233016 32.0 23.5 34.5 2.0 35.0 88-89 25.480902510757705 31.5 21.0 34.0 2.0 35.0 90-91 25.02936857656512 31.0 19.5 34.0 2.0 35.0 92-93 24.479039351495825 31.0 17.5 34.0 2.0 35.0 94-95 23.144102188795582 30.0 4.5 34.0 2.0 35.0 96-97 22.345135620563195 29.5 2.0 34.0 2.0 35.0 98-99 22.00810245709735 30.0 2.0 34.0 2.0 35.0 100-101 20.771982374111783 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.35083463096076173 1101 2 0.24486298438250387 1101 3 0.30607139097433134 1101 4 0.265789035942845 1101 5 0.33706808194153837 1101 6 0.5941922209717632 1101 7 0.488745718583921 1101 8 0.413513271832997 1101 9 0.6909570861473782 1101 10-11 0.6622151755892887 1101 12-13 0.6789820798597432 1101 14-15 0.81736598669508 1101 16-17 0.5741360486112796 1101 18-19 0.723785373870605 1101 20-21 0.7760121921556689 1101 22-23 0.770085148610363 1101 24-25 0.7230550622403698 1101 26-27 0.7214465836839068 1101 28-29 0.6328876158566388 1101 30-31 0.7993612354669466 1101 32-33 0.7614471447981899 1101 34-35 0.6332928848154467 1101 36-37 0.6789765103964598 1101 38-39 0.5803416949328977 1101 40-41 0.7689423627662748 1101 42-43 0.7271960125865675 1101 44-45 0.687278727129403 1101 46-47 0.48337766808005256 1101 48-49 0.46070341895110545 1101 50-51 0.5472105855550353 1101 52-53 0.6427213061279531 1101 54-55 0.5948122283357264 1101 56-57 0.4864654472389134 1101 58-59 0.4380035014856496 1101 60-61 0.13839783977895692 1101 62-63 0.24810953443852668 1101 64-65 0.04981445469090673 1101 66-67 -2.967317753752905 1101 68-69 -7.774159007265268 1101 70-71 -11.083905487401077 1101 72-73 -11.045242186506508 1101 74-75 -9.431162942837929 1101 76-77 -4.641996665956842 1101 78-79 -1.7509101857286886 1101 80-81 -1.0897050561182624 1101 82-83 -0.8856771890262536 1101 84-85 -1.0034193494118568 1101 86-87 -0.7672018469846655 1101 88-89 -0.7104359234161954 1101 90-91 -0.8679115341780523 1101 92-93 -1.458888651956574 1101 94-95 -4.822395692793627 1101 96-97 -1.9565896237218467 1101 98-99 -1.1825115515671456 1101 100-101 -0.6669706700299436 1102 1 0.4499150410924706 1102 2 0.31561354566933986 1102 3 0.32965038074451414 1102 4 0.24279774469273718 1102 5 0.42714813250368877 1102 6 0.4258282562664206 1102 7 0.43716571312241825 1102 8 0.5721449181549474 1102 9 0.6109585750865989 1102 10-11 0.6509104354493402 1102 12-13 0.6545907378250675 1102 14-15 0.7305078523297439 1102 16-17 0.7277406716131054 1102 18-19 0.6853274947873018 1102 20-21 0.8776972419702673 1102 22-23 0.8977299464049935 1102 24-25 0.7611201640998857 1102 26-27 0.8977970700913147 1102 28-29 0.8664754589459207 1102 30-31 0.6447629308806455 1102 32-33 0.5254785434990481 1102 34-35 0.7210668558101645 1102 36-37 1.0364629400437764 1102 38-39 0.8893026068803636 1102 40-41 0.8738302536746048 1102 42-43 1.0751422649697915 1102 44-45 0.8140275356691191 1102 46-47 0.7294768481131939 1102 48-49 0.42220437780324716 1102 50-51 0.551741347286459 1102 52-53 0.7073840521272743 1102 54-55 0.48947155471738313 1102 56-57 0.44592552451548073 1102 58-59 0.4266843198708976 1102 60-61 0.3059491433112633 1102 62-63 0.2596516708006469 1102 64-65 0.25968121007611344 1102 66-67 -2.448303273973444 1102 68-69 -7.362605858313273 1102 70-71 -10.49949159195303 1102 72-73 -8.870759129522764 1102 74-75 -7.399837067174854 1102 76-77 -3.750769854177296 1102 78-79 -1.1574510617439167 1102 80-81 -0.5531048758392316 1102 82-83 -0.38942749330118076 1102 84-85 -0.33079043878404235 1102 86-87 -0.31355233957024353 1102 88-89 -0.44557533693477325 1102 90-91 -0.5035175231734925 1102 92-93 -0.9423665236013612 1102 94-95 -4.260800563169422 1102 96-97 -1.1600184159759053 1102 98-99 -0.5118702357897007 1102 100-101 -0.2156928080231033 1103 1 0.32499929779515924 1103 2 0.20834830496851708 1103 3 0.3443968056169737 1103 4 0.1740088304116938 1103 5 0.4128564835594659 1103 6 0.4103463499803013 1103 7 0.42091753643145324 1103 8 0.3609608246188927 1103 9 0.5044719203870898 1103 10-11 0.6345854364706298 1103 12-13 0.5576017359530994 1103 14-15 0.7177761539068612 1103 16-17 0.6428835085017397 1103 18-19 0.4641166283460336 1103 20-21 0.5649272750842016 1103 22-23 0.5972494428953539 1103 24-25 0.6791849513464499 1103 26-27 0.8189529218619995 1103 28-29 0.6935647848682649 1103 30-31 0.716941938375733 1103 32-33 0.7324796146555599 1103 34-35 0.9435206963538292 1103 36-37 0.8901079961428238 1103 38-39 0.9226359702517399 1103 40-41 0.9829236095004248 1103 42-43 1.0262552043962856 1103 44-45 0.9247033705464034 1103 46-47 1.0162170509060147 1103 48-49 0.8171352182195548 1103 50-51 0.8218593246930439 1103 52-53 0.7754474726430161 1103 54-55 0.727913968189803 1103 56-57 1.0667413757094337 1103 58-59 1.028220330074216 1103 60-61 0.6145032712588971 1103 62-63 0.667489481604246 1103 64-65 0.579369838755472 1103 66-67 -2.7572815390483356 1103 68-69 -7.465300435726107 1103 70-71 -11.209489107807107 1103 72-73 -9.088552868513823 1103 74-75 -7.272021451479137 1103 76-77 -3.7463419466752335 1103 78-79 -0.9860287725568924 1103 80-81 -0.29095914448504345 1103 82-83 -0.006264655883875037 1103 84-85 -0.10885385329217456 1103 86-87 0.16516773653413352 1103 88-89 0.26075258174143556 1103 90-91 0.2713421090647756 1103 92-93 0.30904331181420375 1103 94-95 1.0796516354949688 1103 96-97 1.1875265149245884 1103 98-99 0.6393173031095607 1103 100-101 0.5175090187703475 1104 1 0.4075879620231966 1104 2 0.2567271945294678 1104 3 0.3384736559486825 1104 4 0.19893514357355713 1104 5 0.4954379471192851 1104 6 0.5235012507550891 1104 7 0.5643308451865039 1104 8 0.41350710091333553 1104 9 0.7762299395312624 1104 10-11 0.7999022778735068 1104 12-13 0.6293428935325238 1104 14-15 0.8472270669515893 1104 16-17 0.8154955268970525 1104 18-19 0.7758506572639163 1104 20-21 0.8493353681552378 1104 22-23 0.712056596999254 1104 24-25 0.8225580563705961 1104 26-27 1.020361612588495 1104 28-29 1.1138089835298999 1104 30-31 1.1434699201724428 1104 32-33 0.8509699105550084 1104 34-35 0.8641738329649087 1104 36-37 1.14939805826058 1104 38-39 1.1231715799563204 1104 40-41 1.3005306834368824 1104 42-43 1.325238350032194 1104 44-45 1.0025068907130716 1104 46-47 1.0762577226803458 1104 48-49 1.0828206738298363 1104 50-51 0.9828830676963989 1104 52-53 0.9954479886909056 1104 54-55 0.9699458287464608 1104 56-57 0.9810924274870416 1104 58-59 1.1593982033808636 1104 60-61 0.9366158633074413 1104 62-63 0.9675434266103444 1104 64-65 0.6631187106507817 1104 66-67 -2.3290184962379605 1104 68-69 -6.985187181216091 1104 70-71 -8.281199662546587 1104 72-73 -7.745506733832524 1104 74-75 -6.6114423497385 1104 76-77 -3.154987068942578 1104 78-79 -0.34948970173111604 1104 80-81 0.3317900466398953 1104 82-83 0.47407631975866593 1104 84-85 0.5995547870296463 1104 86-87 0.6431093895195339 1104 88-89 0.7387098361746496 1104 90-91 0.6169903852248027 1104 92-93 0.6613042016247803 1104 94-95 1.5575533847773038 1104 96-97 1.5816922935088407 1104 98-99 1.204500452105787 1104 100-101 1.195262314276171 1105 1 0.5239296830997127 1105 2 0.38209550151299965 1105 3 0.5604270829365738 1105 4 0.411126581476708 1105 5 0.35410629072919164 1105 6 0.5710080841474507 1105 7 0.5289891150290771 1105 8 0.5136917462882735 1105 9 0.7716130484145225 1105 10-11 0.9241974471267298 1105 12-13 0.8454905856166874 1105 14-15 1.0875288713304059 1105 16-17 1.1378174122171316 1105 18-19 0.9999723052692957 1105 20-21 1.0347218218440588 1105 22-23 1.1785043378176852 1105 24-25 1.1714526056996704 1105 26-27 1.2268491732414688 1105 28-29 1.3360310544034562 1105 30-31 1.2232596514680196 1105 32-33 1.2408995485592698 1105 34-35 1.58238289390696 1105 36-37 1.437003132073329 1105 38-39 1.2186528384507653 1105 40-41 1.3686957019165575 1105 42-43 1.5490763706337773 1105 44-45 1.4380898706472678 1105 46-47 1.471823887777532 1105 48-49 1.278894008313408 1105 50-51 1.3788133776887364 1105 52-53 1.507168126841968 1105 54-55 1.524217115179873 1105 56-57 1.6295324010918222 1105 58-59 1.7498479510826321 1105 60-61 1.7207773008776321 1105 62-63 1.778463081656458 1105 64-65 1.6614813450862371 1105 66-67 2.207859271550806 1105 68-69 -4.936064473770038 1105 70-71 -4.973439562293226 1105 72-73 -1.527542630709597 1105 74-75 1.171972182155205 1105 76-77 1.3032940491002982 1105 78-79 1.2507727368591617 1105 80-81 1.363636000938648 1105 82-83 1.5674653342669487 1105 84-85 1.4313367965161845 1105 86-87 1.5414155709018296 1105 88-89 1.7002083998757875 1105 90-91 1.7710257190848253 1105 92-93 1.8881216721042229 1105 94-95 2.5350480648716207 1105 96-97 2.561903228147404 1105 98-99 2.381152498491126 1105 100-101 2.3889289022415277 1106 1 0.11617160888356537 1106 2 0.0960593849387088 1106 3 0.15876916638503857 1106 4 0.03801896318761777 1106 5 0.2805565613596883 1106 6 0.3553407727354596 1106 7 0.2644148907552264 1106 8 0.11173150377734231 1106 9 0.483021789860814 1106 10-11 0.4452196664593373 1106 12-13 0.2495516684637451 1106 14-15 0.35677858282042507 1106 16-17 0.26512183495334796 1106 18-19 0.21875227373637074 1106 20-21 0.391442885511303 1106 22-23 0.397886520090978 1106 24-25 0.5886973759481933 1106 26-27 0.5746971325412531 1106 28-29 0.6817277857071886 1106 30-31 0.5767949147029086 1106 32-33 0.6369676856787265 1106 34-35 0.7268337496844737 1106 36-37 0.679579891984254 1106 38-39 0.6890850345400281 1106 40-41 0.7668159171206241 1106 42-43 0.8195353859613803 1106 44-45 0.5762455197541101 1106 46-47 0.533547958450292 1106 48-49 0.367937943102028 1106 50-51 0.4335067443971212 1106 52-53 0.6978911084911132 1106 54-55 0.7033111125467144 1106 56-57 0.4838844320396021 1106 58-59 0.5983273815395371 1106 60-61 0.41945421845316844 1106 62-63 0.46485109914585365 1106 64-65 0.6558623083032451 1106 66-67 1.3756046675815554 1106 68-69 -5.267260936881392 1106 70-71 -6.29787188841517 1106 72-73 -2.4571567587592718 1106 74-75 0.2795490233365534 1106 76-77 0.5547244084780907 1106 78-79 0.5713113971975687 1106 80-81 0.665948144364684 1106 82-83 0.7245301718073804 1106 84-85 0.6635507652758328 1106 86-87 0.6223121461663652 1106 88-89 0.5898382943867873 1106 90-91 0.6457928793338397 1106 92-93 0.6848749188574708 1106 94-95 1.5203714398695922 1106 96-97 1.6467446695595598 1106 98-99 1.2642726324247775 1106 100-101 1.0010227533216138 1107 1 0.015646040937181027 1107 2 0.016237246293041352 1107 3 -0.08151875082595339 1107 4 -0.0019056672347517178 1107 5 0.13511949465565465 1107 6 0.1710302115018223 1107 7 0.10887992798044621 1107 8 0.11094948037088415 1107 9 0.07028731468290061 1107 10-11 0.12706866210923096 1107 12-13 0.2670960396280364 1107 14-15 0.21729054446360863 1107 16-17 0.22544936564287354 1107 18-19 0.3546516684592973 1107 20-21 0.2887259192196794 1107 22-23 0.3807620909756082 1107 24-25 0.4024568442325531 1107 26-27 0.2821561139821327 1107 28-29 0.33132479503270673 1107 30-31 0.2829600165693691 1107 32-33 0.4155236696645659 1107 34-35 0.5158642516109069 1107 36-37 0.5206945088907275 1107 38-39 0.31292605023624986 1107 40-41 0.3740757010772384 1107 42-43 0.47574441072244156 1107 44-45 0.2984380331230909 1107 46-47 0.29999696946944 1107 48-49 0.5150203478767423 1107 50-51 0.38093684069599476 1107 52-53 0.3958461700208389 1107 54-55 0.36933212019667394 1107 56-57 0.43396512058183134 1107 58-59 0.28826218278655347 1107 60-61 0.3282746589478158 1107 62-63 0.4295814668173996 1107 64-65 0.25253426379872934 1107 66-67 0.872465423718328 1107 68-69 -5.269940308791536 1107 70-71 -5.923840715967703 1107 72-73 -2.303252518203447 1107 74-75 0.1308053838689318 1107 76-77 0.003500443905711137 1107 78-79 0.022077373302913372 1107 80-81 0.07539668978608205 1107 82-83 0.17735969522513884 1107 84-85 0.32649777301922 1107 86-87 0.43127437549058456 1107 88-89 0.23738426274723423 1107 90-91 0.13016717773528796 1107 92-93 0.29495330201277525 1107 94-95 1.2321871505540791 1107 96-97 1.3583739151254548 1107 98-99 0.9063700523502547 1107 100-101 0.8610694185718977 1108 1 0.08997462925425026 1108 2 -0.018761597800274643 1108 3 0.04661045093349614 1108 4 0.008670685620643326 1108 5 0.06662317074817992 1108 6 0.2350136283001234 1108 7 0.14041043347892668 1108 8 0.05952980274127384 1108 9 0.23778894029748443 1108 10-11 0.2778350011788078 1108 12-13 0.32440218170636115 1108 14-15 0.25537801760521006 1108 16-17 0.10973429238777044 1108 18-19 0.3795318059619319 1108 20-21 0.3142562252372727 1108 22-23 0.2865529128445843 1108 24-25 0.4322856540661846 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82-83 -7.049393412777967 2315 84-85 -4.9607489546121215 2315 86-87 -5.248807134879389 2315 88-89 -3.8998856254841954 2315 90-91 -3.8257290627763396 2315 92-93 -4.50253489355234 2315 94-95 -2.4547394378148155 2315 96-97 -1.085498985478214 2315 98-99 -2.447503744792776 2315 100-101 -1.9588225484354531 2316 1 -0.44403767567592567 2316 2 -0.3523656510376263 2316 3 -0.9477207868901409 2316 4 -0.3278648110408895 2316 5 -0.3292823001891634 2316 6 -2.299552897206091 2316 7 -0.447507933342699 2316 8 -0.4607363840120229 2316 9 -0.02608333210340419 2316 10-11 0.019912757226300926 2316 12-13 -0.6399743322113594 2316 14-15 -0.9219625119261394 2316 16-17 -1.5294044322131342 2316 18-19 -0.15462434964025107 2316 20-21 -3.925584136714072 2316 22-23 -4.290491791064348 2316 24-25 -2.6572475136355465 2316 26-27 -2.064011627918539 2316 28-29 -1.313706328741965 2316 30-31 -0.13928073331528168 2316 32-33 -1.705922324447947 2316 34-35 -2.228213596949736 2316 36-37 -4.635999193772669 2316 38-39 -2.198153581836749 2316 40-41 -1.8661632787860682 2316 42-43 -3.1446348487286926 2316 44-45 -0.8563499285732163 2316 46-47 -0.7877962240429994 2316 48-49 -0.368484321364015 2316 50-51 0.09150948037522966 2316 52-53 -2.598576104186769 2316 54-55 -0.9345226678999268 2316 56-57 0.023835930609436673 2316 58-59 0.026432450205479796 2316 60-61 -1.2003647450730455 2316 62-63 -0.7791350788348517 2316 64-65 0.2074239477581834 2316 66-67 1.1248718163015106 2316 68-69 3.7181708679758536 2316 70-71 3.826317533653871 2316 72-73 1.9589650810971797 2316 74-75 1.03932037373718 2316 76-77 -0.21246428518118776 2316 78-79 0.18869196464653726 2316 80-81 -0.4471863271829193 2316 82-83 -0.18759873895353962 2316 84-85 0.18198308534974217 2316 86-87 -0.19272951531852556 2316 88-89 0.051028267204664246 2316 90-91 0.31719124840662616 2316 92-93 0.6242614557132278 2316 94-95 1.0341046287053786 2316 96-97 1.1258872779771352 2316 98-99 -0.0018234465987987392 2316 100-101 -0.15488802536617996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 30.0 4 74.0 5 259.0 6 592.0 7 1383.0 8 3057.0 9 4984.0 10 8078.0 11 11726.0 12 15157.0 13 19132.0 14 23151.0 15 29330.0 16 35248.0 17 39320.0 18 34143.0 19 27355.0 20 25696.0 21 25629.0 22 27417.0 23 30622.0 24 36690.0 25 45473.0 26 56399.0 27 69499.0 28 84561.0 29 100159.0 30 117767.0 31 136161.0 32 158178.0 33 181840.0 34 211925.0 35 241370.0 36 247917.0 37 203925.0 38 81779.0 39 4610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.84715629481616 20.788798504005783 15.983481160762972 21.38056404041509 2 16.025235352910787 21.813949095999547 38.89379111011025 23.267024440979416 3 18.714902881595183 26.670990255689876 30.81458638804297 23.79952047467197 4 12.988625372119172 18.504063831896126 36.32306096431416 32.18424983167055 5 12.831505845610932 38.836303668433274 33.54421537653083 14.787975109424966 6 30.362660254767054 37.58869338747219 16.97034325537202 15.078303102388741 7 27.345034366551484 34.72226494543391 20.43489666109556 17.49780402691904 8 26.983766888485583 34.751615791866186 18.980854874376668 19.283762445271567 9 26.93617323066091 16.724428448873986 20.412552421380745 35.926845899084356 10-11 24.98649946510539 27.6314294161275 26.11452042340412 21.267550695362996 12-13 26.231731554680582 25.469976690215702 27.298530150624956 20.99976160447876 14-15 23.09734312891131 26.278385301848033 26.627519387793463 23.99675218144719 16-17 21.075556028360612 29.592455569319593 27.51736116674047 21.81462723557932 18-19 20.793412524483326 26.778121299730607 31.582268570746212 20.846197605039855 20-21 21.72919635929879 25.91186286874404 31.892834804720742 20.466105967236423 22-23 21.406892450295818 25.95482105810015 31.906869379761503 20.731417111842532 24-25 21.182317888037844 26.52682077783588 31.862608132448795 20.428253201677485 26-27 20.87941501357217 26.623880565769426 31.798949473337473 20.69775494732093 28-29 20.481256629964395 26.463538267112916 32.32454964314367 20.73065545977902 30-31 20.73440967739799 26.45931195983502 32.02000045715819 20.786277905608795 32-33 20.618096178487914 26.63257366817905 31.732744104687065 21.016586048645973 34-35 21.2515162792526 26.666411728086214 31.413610216571037 20.66846177609015 36-37 21.47728373472683 26.247967800421808 31.35246396781582 20.922284497035548 38-39 20.944132514448487 26.637992439121973 31.46764815379901 20.95022689263053 40-41 20.97133321028736 26.40016305374165 31.8561114890652 20.772392246905795 42-43 21.118433587392538 26.595365007230882 31.118970317134576 21.167231088242005 44-45 20.95306804244335 26.938534804675406 30.960513418783385 21.147883734097856 46-47 21.393358546500203 27.305017237740547 29.9990784015163 21.30254581424295 48-49 21.440452986893018 27.48405596902822 29.60103838007576 21.474452664003003 50-51 21.51226442367729 27.56056943738806 29.392962774792714 21.534203364141934 52-53 21.816833823108762 27.60110089373861 29.31950503393368 21.262560249218946 54-55 21.338466911973747 27.58531133008338 29.423169249487735 21.653052508455144 56-57 21.41966035340854 28.063860568385557 28.956442579125856 21.56003649908004 58-59 21.42625028815867 27.49363807612773 29.223807899062106 21.856303736651498 60-61 21.330501947823745 27.761556891728347 29.42840692906119 21.479534231386722 62-63 21.46407831864759 27.687461120947177 29.55280117166939 21.295659388735842 64-65 21.15199130456517 27.521572492825904 29.45070119547275 21.87573500713618 66-67 20.851488903312767 27.73358855878415 29.627273316170537 21.787649221732543 68-69 21.071997057653117 27.33064278565141 30.1163544873374 21.481005669358076 70-71 20.661494918772778 27.52065595591557 30.183127635875845 21.634721489435808 72-73 21.207663179288048 27.824843085098404 28.910191682299963 22.05730205331358 74-75 20.874789385995463 28.188817968743983 28.94520998077198 21.991182664488576 76-77 21.11083218409468 28.12932433313001 28.914645128278533 21.845198354496773 78-79 21.107735468184774 28.13212533692636 28.750892719997935 22.009246474890933 80-81 21.071350295030307 28.74912362476913 28.389815120607604 21.789710959592963 82-83 21.321275134065438 28.562667261373775 28.16922920490394 21.946828399656848 84-85 21.019991045214827 28.36165455036383 28.151499072051845 22.466855332369498 86-87 21.21206813167658 28.22445446637763 28.384154104596853 22.179323297348937 88-89 21.091093967122134 28.367310972568312 28.14038513094217 22.401209929367383 90-91 20.96139664885622 28.62052214542245 28.288060962823646 22.130020242897682 92-93 21.07425437690482 28.290024457304817 28.292865597614636 22.342855568175725 94-95 20.47666308863569 27.99459570319441 28.631041352589904 22.897699855579994 96-97 20.671113006571883 27.77365286691296 28.59130582974081 22.96392829677435 98-99 20.775278063883604 27.91432164931979 28.059535933859024 23.250864352937583 100-101 20.64043977200457 27.756954700210706 28.41812094114661 23.184484586638117 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6578.0 1 4289.0 2 2104.0 3 2228.0 4 2108.0 5 2051.5 6 2971.0 7 5455.0 8 8079.0 9 9337.5 10 8520.0 11 6787.5 12 6115.0 13 5937.5 14 5826.5 15 5866.0 16 5980.5 17 6204.5 18 6553.5 19 7020.0 20 7593.0 21 8606.0 22 9776.5 23 11039.0 24 12414.5 25 14355.0 26 17270.0 27 20329.0 28 23374.5 29 26708.0 30 30529.0 31 35102.5 32 41546.0 33 47955.0 34 52638.5 35 56934.5 36 63494.5 37 69423.5 38 70995.0 39 70964.5 40 70225.5 41 70286.0 42 70917.5 43 71932.0 44 73094.5 45 74424.0 46 74117.0 47 73675.5 48 77361.5 49 76845.0 50 71616.5 51 68359.0 52 66751.5 53 67317.5 54 67580.0 55 64491.5 56 60157.5 57 55434.0 58 50866.5 59 45508.5 60 38913.0 61 33253.0 62 28096.0 63 22656.0 64 17803.5 65 13845.5 66 10438.5 67 7885.0 68 5429.5 69 3403.5 70 2426.0 71 1905.0 72 1550.5 73 1273.0 74 994.5 75 777.0 76 531.0 77 298.5 78 177.5 79 102.5 80 62.5 81 37.5 82 24.0 83 16.0 84 8.5 85 2.5 86 1.0 87 1.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022728748620040262 2 0.018755489932702398 3 0.0 4 0.0 5 1.28169635075415E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.2042408768853756E-4 18-19 0.0010467186864492226 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.340401461475625E-4 28-29 0.017965110516404002 30-31 0.004336405986718208 32-33 0.007989240586367536 34-35 0.009185490513738076 36-37 0.1006772483517385 38-39 0.10371059638185663 40-41 0.11827921156876216 42-43 0.10311247141817138 44-45 0.09757981550408262 46-47 0.09909648951914171 48-49 0.10212983754925987 50-51 0.09997231535882371 52-53 0.07779896849077692 54-55 0.0657510227936879 56-57 0.03687013169002772 58-59 0.01527354817982029 60-61 0.009206852119583978 62-63 0.00239249985474108 64-65 0.0046781916802526476 66-67 0.4117235910739248 68-69 0.7985822729432192 70-71 0.8859085176412685 72-73 0.27432574227307993 74-75 0.1467115089496584 76-77 0.014120021464141555 78-79 0.008053325403905243 80-81 0.001751651679364005 82-83 0.0 84-85 0.0 86-87 1.7089284676722002E-4 88-89 6.622097812229776E-4 90-91 0.0031615176651935703 92-93 0.0016448436501344925 94-95 0.2178456564165137 96-97 0.22404052211182543 98-99 0.01339372686538087 100-101 0.006942521899918314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2340648.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.62274257935546 #Duplication Level Percentage of deduplicated Percentage of total 1 68.99331522842016 26.64711053763597 2 14.408360815012887 11.129808214974302 3 5.895111121782047 6.8305607799985015 4 3.125195129710459 4.8281442802024985 5 1.8723048941007139 3.615677497745964 6 1.1991194510545549 2.778796912798686 7 0.8528090952261548 2.3056478307976973 8 0.5916663047431391 1.8281420304778195 9 0.4225550693883115 1.4688212103527793 >10 2.2718755575905756 16.056210729972946 >50 0.2126074731286677 5.7567904297370545 >100 0.1429109518749393 10.07272214111778 >500 0.008788655754243327 2.2719548602627717 >1k 0.0029295519180811067 1.8406459434496167 >5k 1.1267507377235026E-4 0.34962524847915216 >10k+ 3.380252213170508E-4 2.21934135199649 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19980 0.853609769602264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18352 0.7840563809680055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12666 0.5411321992884022 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8034 0.34323828273196144 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4738 0.2024225769957721 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3663 0.15649512442708174 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2600 0.11108035039869302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.2723211691805006E-5 0.0 0.0 0.0 0.0 7 4.2723211691805006E-5 0.0 0.0 0.0 0.0 8 4.2723211691805006E-5 0.0 0.0 0.0 0.0 9 4.2723211691805006E-5 0.0 0.0 0.0 0.0 10-11 8.544642338361001E-5 0.0 0.0 0.0 0.0 12-13 8.544642338361001E-5 0.0 0.0 4.2723211691805006E-5 0.0 14-15 8.544642338361001E-5 0.0 0.0 1.2816963507541503E-4 0.0 16-17 8.544642338361001E-5 0.0 0.0 1.495312409213175E-4 0.0 18-19 1.0680802922951251E-4 0.0 0.0 3.6314729938034254E-4 0.0 20-21 1.2816963507541503E-4 0.0 0.0 5.767633578393676E-4 0.0 22-23 1.2816963507541503E-4 0.0 0.0 0.0010894418981410277 0.0 24-25 3.6314729938034254E-4 2.1361605845902503E-5 0.0 0.0015807588325967852 0.0 26-27 3.84508905226245E-4 4.2723211691805006E-5 0.0 0.002371138248895178 0.0 28-29 4.699553286098551E-4 4.2723211691805006E-5 0.0 0.0040587051107214755 0.0 30-31 4.699553286098551E-4 4.2723211691805006E-5 0.0 0.009740892265731542 0.0 32-33 4.699553286098551E-4 4.2723211691805006E-5 0.0 0.01809328015147942 0.0 34-35 5.554017519934651E-4 4.2723211691805006E-5 0.0 0.030611181177178287 0.0 36-37 5.9812496368527E-4 4.2723211691805006E-5 0.0 0.05421575563690055 0.0 38-39 6.622097812229776E-4 4.2723211691805006E-5 0.0 0.0888001955014167 0.0 40-41 6.835713870688801E-4 4.2723211691805006E-5 0.0 0.12492267098683785 0.0 42-43 6.835713870688801E-4 4.2723211691805006E-5 0.0 0.16100242326056716 0.0 44-45 7.6901781045249E-4 4.2723211691805006E-5 0.0 0.20545592502589027 0.0 46-47 8.331026279901977E-4 4.2723211691805006E-5 0.0 0.2630254527805975 0.0 48-49 8.971874455279051E-4 4.2723211691805006E-5 0.0 0.32651214535461975 0.0 50-51 8.971874455279051E-4 4.2723211691805006E-5 0.0 0.3952324313608881 0.0 52-53 8.971874455279051E-4 4.2723211691805006E-5 0.0 0.4612397934247269 0.0 54-55 9.399106572197102E-4 4.2723211691805006E-5 0.0 0.5336983604540281 0.0 56-57 9.826338689115151E-4 4.2723211691805006E-5 0.0 0.6182689579979561 0.0 58-59 9.826338689115151E-4 4.2723211691805006E-5 0.0 0.7018996448846644 0.0 60-61 9.826338689115151E-4 6.408481753770751E-5 0.0 0.7905503091451598 0.0 62-63 9.826338689115151E-4 8.544642338361001E-5 0.0 0.8769580047918355 0.0 64-65 9.826338689115151E-4 1.0680802922951251E-4 0.0 0.9650959905120291 0.0 66-67 9.826338689115151E-4 1.2816963507541503E-4 0.0 1.059108417839846 0.0 68-69 9.826338689115151E-4 1.2816963507541503E-4 0.0 1.1734998171446538 0.0 70-71 0.0010039954747574176 1.2816963507541503E-4 0.0 1.3286491604034438 0.0 72-73 0.0010467186864492226 1.2816963507541503E-4 0.0 1.4989866054186702 0.0 74-75 0.001068080292295125 1.2816963507541503E-4 0.0 1.6807311479556089 0.0 76-77 0.001068080292295125 1.2816963507541503E-4 0.0 1.8830041937104598 0.0 78-79 0.001068080292295125 1.2816963507541503E-4 0.0 2.1134745591819017 0.0 80-81 0.00119624992737054 1.2816963507541503E-4 0.0 2.3772049449554142 0.0 82-83 0.00119624992737054 1.2816963507541503E-4 0.0 2.6463611786137857 0.0 84-85 0.0012816963507541503 1.2816963507541503E-4 0.0 2.9147056712500126 0.0 86-87 0.0013244195624459551 1.2816963507541503E-4 0.0 3.1856990030111323 0.0 88-89 0.0013244195624459551 1.2816963507541503E-4 0.0 3.476280927332944 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10720 0.0 46.553673 1 GTATCAA 17860 0.0 36.61997 1 TATCAAC 22660 0.0 28.667284 2 ATCAACG 22940 0.0 28.210491 3 TCAACGC 23115 0.0 28.017406 4 CAACGCA 23445 0.0 27.60284 5 AACGCAG 24060 0.0 26.976044 6 ACGCAGA 27210 0.0 23.748663 7 CGCAGAG 27350 0.0 23.609776 8 GAGTACT 16180 0.0 23.439842 12-13 AGGTAAG 935 0.0 23.310696 2 GTACATG 15080 0.0 23.068274 1 GGTAAGG 1000 0.0 22.740215 3 GCAGAGT 28155 0.0 22.295317 9 TAAGGTG 1255 0.0 22.272123 5 TACATGG 15565 0.0 22.069773 2 GTACTTT 17305 0.0 21.847574 14-15 TGGTATC 5785 0.0 21.458874 2 ACATGGG 14865 0.0 21.353212 3 AGTACTT 16955 0.0 21.055101 12-13 >>END_MODULE