FastQCFastQC Report
Fri 27 May 2016
SRR939546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325520
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136750.5880405242698408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105630.4542209914341739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74870.3219494994667859No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51610.22192885892187553No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.11494203446971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93200.041.742911
GTATCAA156950.039.4423641
TATCAAC198900.030.76542
ATCAACG207400.029.7702833
TCAACGC211450.029.2706454
CAACGCA212200.028.9380025
AACGCAG216150.028.2045656
GTACATG138500.027.8354421
TACATGG144950.025.9843882
AACGCGG3600.025.5970826
ACGCAGA238750.025.0268787
CGCAGAG242800.024.5090038
ACATGGG144600.024.0954673
CAACGCG3650.023.9388375
GAGTACT127200.023.73557912-13
GCAGAGT238050.023.0615659
CATGGGG54250.021.8859254
GTACTTT141150.021.88411514-15
AGTACTT134950.021.695612-13
CATGGGA87000.021.3752234
Produced by FastQC (version 0.11.5)