FastQCFastQC Report
Fri 27 May 2016
SRR939546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325520
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195300.8398121710413156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179030.7698493240221542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125920.5414702948157831No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81080.34865320444459735No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.19690219821803298No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36170.15553510612680174No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.10999690391826344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104950.046.7659651
GTATCAA173800.036.3240051
TATCAAC226050.027.7935752
ATCAACG229550.027.4270943
TCAACGC232150.027.1280924
CAACGCA235300.026.7004475
AACGCAG241300.026.016886
ACGCAGA270400.023.1117747
TAAGGTA4950.023.0103424
CGCAGAG271600.022.99228
GTACATG153200.022.9279271
GAGTACT159150.022.5913812-13
TAAGGTG11850.022.0074065
TACATGG157900.021.8480762
GCAGAGT278450.021.5581289
AGTACTT167950.021.54878612-13
GTACTTT170400.021.21114214-15
ACATGGG155400.020.821863
GGTAAGG10850.020.552013
GTAAGGT12050.020.0863114
Produced by FastQC (version 0.11.5)