##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939546_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2325520 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.863374213079226 31.0 31.0 31.0 28.0 34.0 2 31.106323316935566 31.0 31.0 34.0 28.0 34.0 3 31.08322568715814 31.0 31.0 34.0 27.0 34.0 4 35.016166276789704 35.0 35.0 37.0 32.0 37.0 5 34.58889452681551 35.0 35.0 37.0 30.0 37.0 6 34.6664268636692 35.0 35.0 37.0 31.0 37.0 7 34.50264972995287 35.0 35.0 37.0 30.0 37.0 8 34.40342418039836 35.0 35.0 37.0 30.0 37.0 9 35.86984502390863 37.0 35.0 39.0 30.0 39.0 10-11 35.672480133475524 37.0 35.0 39.0 30.0 39.0 12-13 35.64862869379752 37.0 35.0 39.0 30.0 39.0 14-15 36.41335529257972 38.0 35.0 40.0 30.0 41.0 16-17 36.34187106539613 38.0 34.5 40.0 30.0 41.0 18-19 36.408049812515046 38.0 35.0 40.0 30.0 41.0 20-21 36.319262788537614 38.0 34.5 40.0 29.5 41.0 22-23 36.16379433417042 38.0 34.0 40.0 29.0 41.0 24-25 35.98234201382917 38.0 34.0 40.0 29.0 41.0 26-27 35.49019015101999 38.0 34.0 40.0 27.0 41.0 28-29 35.26808455743232 38.0 33.5 40.0 27.0 41.0 30-31 34.90884081839761 37.0 33.0 40.0 26.5 41.0 32-33 34.562782087447104 37.0 33.0 40.0 25.0 41.0 34-35 34.71754553820221 37.0 33.0 40.0 25.5 41.0 36-37 34.479861708349105 37.0 33.0 40.0 25.0 41.0 38-39 34.25486106333207 37.0 33.0 40.0 24.0 41.0 40-41 34.04489920533902 37.0 33.0 40.0 24.0 41.0 42-43 33.8085299201899 37.0 33.0 40.0 23.5 41.0 44-45 33.32747149024735 37.0 32.0 40.0 21.5 41.0 46-47 32.794114004609725 36.0 31.0 39.5 19.0 41.0 48-49 32.29467280436204 36.0 30.5 39.0 15.5 40.5 50-51 32.01365780556607 36.0 30.0 39.0 14.0 40.0 52-53 31.86847092263236 36.0 30.0 39.0 12.5 40.0 54-55 31.510239860332312 35.0 30.0 39.0 8.5 40.0 56-57 31.015897949705874 35.0 28.5 39.0 8.0 40.0 58-59 30.54675040421067 35.0 28.0 38.0 7.0 40.0 60-61 30.16637719735801 34.0 28.0 38.0 6.5 40.0 62-63 29.991954057587122 34.0 28.0 38.0 2.0 40.0 64-65 29.530366111665348 34.0 27.5 37.5 2.0 40.0 66-67 28.676088788744025 33.5 26.0 36.5 2.0 39.0 68-69 25.352892471361244 31.0 15.5 35.0 2.0 38.5 70-71 24.073947117203893 29.0 10.5 35.0 2.0 38.0 72-73 25.0309272764801 30.0 16.0 35.0 2.0 37.5 74-75 25.73560644501015 31.0 20.5 35.0 2.0 37.0 76-77 25.394032517458463 30.5 21.0 34.0 2.0 36.0 78-79 26.460285226529983 31.5 23.5 35.0 2.0 36.0 80-81 26.475322293508547 32.0 24.0 35.0 2.0 36.0 82-83 26.153812480649492 32.0 23.5 35.0 2.0 36.0 84-85 25.797951640923323 32.0 22.5 34.0 2.0 35.5 86-87 25.351601362275964 31.0 20.0 34.0 2.0 35.0 88-89 24.85547490453748 31.0 19.0 34.0 2.0 35.0 90-91 24.25950518593691 31.0 17.0 34.0 2.0 35.0 92-93 23.705508445422957 30.5 9.0 34.0 2.0 35.0 94-95 22.874125141903747 30.0 2.0 34.0 2.0 35.0 96-97 22.21134412948502 29.5 2.0 34.0 2.0 35.0 98-99 21.36719486394441 29.0 2.0 34.0 2.0 35.0 100-101 19.991353976744985 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2802820161047599 1101 2 0.3103181499051182 1101 3 0.3560318459571583 1101 4 0.36458412982602795 1101 5 0.6496004133405862 1101 6 0.3586610060256703 1101 7 0.5401371008728617 1101 8 0.6906165397978441 1101 9 0.6277250064286122 1101 10-11 0.7459733089199645 1101 12-13 0.6396428805870684 1101 14-15 0.8199312490187793 1101 16-17 0.7422991379534167 1101 18-19 0.461824458898775 1101 20-21 0.568863057672317 1101 22-23 0.5318818242879502 1101 24-25 0.6855242464518838 1101 26-27 0.5755924367913892 1101 28-29 0.5722020861082697 1101 30-31 0.5461194895487935 1101 32-33 0.8705290921143245 1101 34-35 0.7283420596283676 1101 36-37 0.9252282466900255 1101 38-39 0.893108460437233 1101 40-41 0.9550713374982891 1101 42-43 0.6747215217025797 1101 44-45 0.8103771682260614 1101 46-47 0.9263091127622474 1101 48-49 0.960976278900759 1101 50-51 0.44345647204159633 1101 52-53 0.2875990735772369 1101 54-55 0.41670379237829636 1101 56-57 0.7445009181737987 1101 58-59 0.5023841916072627 1101 60-61 0.488303923818755 1101 62-63 0.2489235223477415 1101 64-65 0.035256669635788995 1101 66-67 -0.21367647710216175 1101 68-69 -7.353427594043737 1101 70-71 -7.660233091549657 1101 72-73 -4.737004489401038 1101 74-75 -2.659812034271159 1101 76-77 -0.019669948350848898 1101 78-79 0.23655314920989312 1101 80-81 0.25710318913678165 1101 82-83 0.06846174254192405 1101 84-85 -0.12419284175587819 1101 86-87 -0.2792871317263632 1101 88-89 -0.2671033218938135 1101 90-91 -0.60584944478164 1101 92-93 -0.374655310548178 1101 94-95 -0.22448720840433367 1101 96-97 -0.42775377863038244 1101 98-99 -0.598302087250044 1101 100-101 -0.5587133573938914 1102 1 0.3249595498398037 1102 2 0.34284862708095787 1102 3 0.4010489364595209 1102 4 0.39206413040187016 1102 5 0.46848052069685053 1102 6 0.5245687554934904 1102 7 0.5714799117519433 1102 8 0.5678033949138239 1102 9 0.5568114704127467 1102 10-11 0.5069283178047712 1102 12-13 0.6263605777850572 1102 14-15 0.7939102471980064 1102 16-17 0.6640798111959043 1102 18-19 0.4485285425305108 1102 20-21 0.6573196297700719 1102 22-23 0.685809166024768 1102 24-25 0.46127970549195396 1102 26-27 0.6422885838704815 1102 28-29 0.6170353152274046 1102 30-31 0.5001818403081231 1102 32-33 0.7797596220544705 1102 34-35 0.6808908008898698 1102 36-37 0.8893577514503903 1102 38-39 1.0325285913653062 1102 40-41 0.9374104736533582 1102 42-43 0.8417372902807188 1102 44-45 1.0029063247294872 1102 46-47 1.1515450968916454 1102 48-49 1.2864902614415996 1102 50-51 0.9160482947635415 1102 52-53 0.6221327479069281 1102 54-55 0.6204938718126733 1102 56-57 0.9254838927661915 1102 58-59 0.7985210370529146 1102 60-61 0.8106869426004693 1102 62-63 0.7214264656946519 1102 64-65 0.587574387113289 1102 66-67 0.26244939612205087 1102 68-69 -6.562851186260154 1102 70-71 -7.19025397820279 1102 72-73 -1.1887570717537237 1102 74-75 0.41135515221467145 1102 76-77 0.7965190542660885 1102 78-79 0.5687590601890626 1102 80-81 0.3251247029528308 1102 82-83 0.24766556485578306 1102 84-85 0.08253072648814452 1102 86-87 -0.11930018823174038 1102 88-89 -0.11221817970871939 1102 90-91 -0.09657985798936508 1102 92-93 0.009644241737618131 1102 94-95 0.005329819006188785 1102 96-97 -0.09986868839591168 1102 98-99 -0.16313912190861757 1102 100-101 -0.05031926374131501 1103 1 0.3715120772026985 1103 2 0.22953138670111883 1103 3 0.3232666043330532 1103 4 0.24604234706958295 1103 5 0.3343490083842511 1103 6 0.401024134244949 1103 7 0.26060031221395974 1103 8 0.48168733034379585 1103 9 0.4625911876934339 1103 10-11 0.497512944825381 1103 12-13 0.48946898503670866 1103 14-15 0.7141038042528365 1103 16-17 0.7168743187757869 1103 18-19 0.6353887869850041 1103 20-21 0.5066520479488474 1103 22-23 0.5342423422227114 1103 24-25 0.5033074114126137 1103 26-27 0.63610429322204 1103 28-29 0.6593939198053818 1103 30-31 0.5953508132098833 1103 32-33 0.7039065472782156 1103 34-35 0.5852276569669854 1103 36-37 0.7586493608680698 1103 38-39 0.913332457406085 1103 40-41 0.8844276586138946 1103 42-43 0.5294840259219171 1103 44-45 0.6560973198076852 1103 46-47 0.9523406842460957 1103 48-49 0.8650423061402819 1103 50-51 0.6398194447545578 1103 52-53 0.560901879540534 1103 54-55 0.5941843186067253 1103 56-57 0.6878947693801756 1103 58-59 0.849158638958194 1103 60-61 0.6805089539542735 1103 62-63 0.2989979432255758 1103 64-65 0.35111852942956645 1103 66-67 0.37809801035952617 1103 68-69 -6.911821139791286 1103 70-71 -7.308402992068814 1103 72-73 -0.8696550759745456 1103 74-75 0.4523026170769029 1103 76-77 0.5715473081599001 1103 78-79 0.45644524510792195 1103 80-81 0.26743109147388466 1103 82-83 0.3000638648328291 1103 84-85 0.0038713885218157884 1103 86-87 0.14018127118061585 1103 88-89 0.141549524457929 1103 90-91 -0.272361839313497 1103 92-93 0.03707804179148155 1103 94-95 0.17483016825355335 1103 96-97 -0.08260566812463566 1103 98-99 0.08584345801864757 1103 100-101 -0.13076075843294532 1104 1 0.29956296957795203 1104 2 0.21855789990686958 1104 3 0.18306511498764877 1104 4 0.09143883738212111 1104 5 0.36214263587960005 1104 6 0.3442035273855808 1104 7 0.4373899286292655 1104 8 0.5144072518596943 1104 9 0.6058165004508211 1104 10-11 0.4496522631869695 1104 12-13 0.4087247724370613 1104 14-15 0.5626742572648382 1104 16-17 0.5150737219115769 1104 18-19 0.48985841238445715 1104 20-21 0.5750488176536521 1104 22-23 0.4796714787492675 1104 24-25 0.38874689725820133 1104 26-27 0.630538242378698 1104 28-29 0.43431817665373984 1104 30-31 0.43665638631364345 1104 32-33 0.5572283336088191 1104 34-35 0.2776584725714173 1104 36-37 0.6729528977077379 1104 38-39 0.6400753217791504 1104 40-41 0.6838408137819343 1104 42-43 0.5600280628949292 1104 44-45 0.5994546249769925 1104 46-47 0.5333043544736285 1104 48-49 0.6731126106778476 1104 50-51 0.6273214841969974 1104 52-53 0.350201821815606 1104 54-55 0.31542765050020805 1104 56-57 0.5236361835166967 1104 58-59 0.6195187825470541 1104 60-61 0.7084960068623296 1104 62-63 0.3165721857410908 1104 64-65 0.22684799901712793 1104 66-67 0.08115609943147462 1104 68-69 -6.693220758812846 1104 70-71 -4.266687729411331 1104 72-73 0.1718501127605272 1104 74-75 0.8672028516392665 1104 76-77 0.9716321096143155 1104 78-79 0.5705939356723633 1104 80-81 0.25631790854822967 1104 82-83 0.12967113556998555 1104 84-85 0.20189012505733217 1104 86-87 0.07282904410947211 1104 88-89 0.12970690978223942 1104 90-91 0.010516999795004978 1104 92-93 -0.08072696420349246 1104 94-95 0.1583638840586481 1104 96-97 0.20550640298326783 1104 98-99 0.21660365318761166 1104 100-101 0.3554984697603061 1105 1 0.26149355231151006 1105 2 0.23729931645044644 1105 3 0.18828403483159661 1105 4 0.15018077705118316 1105 5 0.31956913786279273 1105 6 0.32935285667776526 1105 7 0.32915987171113414 1105 8 0.32176495243782455 1105 9 0.49024515007221936 1105 10-11 0.44267002404044575 1105 12-13 0.4933823043220755 1105 14-15 0.3737723794078036 1105 16-17 0.389852861153166 1105 18-19 0.4422009371218252 1105 20-21 0.43287267208684455 1105 22-23 0.5419815949708067 1105 24-25 0.5542633566141006 1105 26-27 0.6763945014637329 1105 28-29 0.6083502124426587 1105 30-31 0.41822200876536186 1105 32-33 0.6283650035613633 1105 34-35 0.5116250849786823 1105 36-37 0.7197453582210542 1105 38-39 0.6311558835505409 1105 40-41 0.813281900266027 1105 42-43 0.5469460616367527 1105 44-45 0.58890138558926 1105 46-47 0.5195792952616145 1105 48-49 0.6876566882359612 1105 50-51 0.244270943289699 1105 52-53 0.3720489987287685 1105 54-55 0.3607630876302572 1105 56-57 0.5668278464419778 1105 58-59 0.5429864149747097 1105 60-61 0.5408239583935632 1105 62-63 0.23247731098877367 1105 64-65 0.14784089119814325 1105 66-67 0.40647439231249294 1105 68-69 -3.0200615400710404 1105 70-71 -3.261380086295265 1105 72-73 0.7573307499095385 1105 74-75 1.079518310577111 1105 76-77 1.0444529612774751 1105 78-79 0.7657985839990253 1105 80-81 0.6858512702076212 1105 82-83 0.5583633352190454 1105 84-85 0.40385624465660186 1105 86-87 0.37731862001669114 1105 88-89 0.384510487306688 1105 90-91 0.6202372212851479 1105 92-93 0.2947319409453826 1105 94-95 0.1092537695610396 1105 96-97 0.2907444258245313 1105 98-99 0.43206811951712965 1105 100-101 0.5694567953842267 1106 1 0.12422787088776133 1106 2 0.1655098896663567 1106 3 0.18643311121127581 1106 4 0.11466514144014894 1106 5 0.2333581370615221 1106 6 0.24704357232631224 1106 7 0.2570834417255412 1106 8 0.32724630935662447 1106 9 0.3544930849067711 1106 10-11 0.36926884297061946 1106 12-13 0.14584118965374415 1106 14-15 0.29940216953165333 1106 16-17 0.3924877661321986 1106 18-19 0.33184730785630023 1106 20-21 0.2582709732434836 1106 22-23 0.2707316803082236 1106 24-25 0.20108952340113717 1106 26-27 0.5051440927424267 1106 28-29 0.3650038359591363 1106 30-31 0.270518438607219 1106 32-33 0.4745346561392765 1106 34-35 0.3047530425938305 1106 36-37 0.33633917368383237 1106 38-39 0.3737977772147332 1106 40-41 0.2780678650459265 1106 42-43 0.31468703653138164 1106 44-45 0.5150373613282895 1106 46-47 0.6380628853258301 1106 48-49 0.7190696153272711 1106 50-51 0.6332036237468941 1106 52-53 0.49368019197939006 1106 54-55 0.3447742246120846 1106 56-57 0.49301232976435116 1106 58-59 0.6216369699373914 1106 60-61 0.6449859090451824 1106 62-63 0.3461222696135984 1106 64-65 0.255621148252402 1106 66-67 0.33759789082312963 1106 68-69 -3.311179576987108 1106 70-71 -3.3588744899574117 1106 72-73 0.5863091802041431 1106 74-75 1.0954261030508938 1106 76-77 0.988801288843792 1106 78-79 0.7677090192378095 1106 80-81 0.6607569049922297 1106 82-83 0.5534294851901187 1106 84-85 0.5362258041718135 1106 86-87 0.5506308256026067 1106 88-89 0.745357277002956 1106 90-91 0.8007855905320582 1106 92-93 0.6478332373554601 1106 94-95 1.0225848661558103 1106 96-97 0.8177984075784934 1106 98-99 0.784735093398492 1106 100-101 0.9972817782269168 1107 1 -0.025197330254336237 1107 2 0.07765242178158616 1107 3 0.09849251126028236 1107 4 -0.10831006145209443 1107 5 0.3426430512792322 1107 6 0.23484003502564121 1107 7 0.1604496028492406 1107 8 0.08858602323230258 1107 9 0.2739574893811536 1107 10-11 0.027451976036928727 1107 12-13 0.26950920143473667 1107 14-15 0.151593355782488 1107 16-17 0.06142056291992759 1107 18-19 0.23008209531323587 1107 20-21 0.3373170671614645 1107 22-23 0.3132240421589785 1107 24-25 0.22481403998504135 1107 26-27 0.49949455003130083 1107 28-29 0.5653389894471843 1107 30-31 0.6183533170718931 1107 32-33 0.33119028436767906 1107 34-35 0.24471075149909183 1107 36-37 0.34786452833898096 1107 38-39 0.4948090006687238 1107 40-41 0.36416799605705563 1107 42-43 0.17933834439255492 1107 44-45 0.43917466299892993 1107 46-47 0.5289301715451558 1107 48-49 0.37198417556489005 1107 50-51 0.4826692658264733 1107 52-53 0.2417597162389491 1107 54-55 0.21322460539444776 1107 56-57 0.6609916080030054 1107 58-59 0.8360908235149509 1107 60-61 0.5542144434706096 1107 62-63 0.3384113016813295 1107 64-65 0.1355346909035262 1107 66-67 0.4943634033916986 1107 68-69 -2.4651273500478226 1107 70-71 -2.7348570734770092 1107 72-73 1.1255637668440066 1107 74-75 1.451627630180699 1107 76-77 0.9806418452910926 1107 78-79 0.9863072875906163 1107 80-81 0.7952411486529414 1107 82-83 0.5697021183639386 1107 84-85 0.49959081519774884 1107 86-87 0.47490563896174365 1107 88-89 0.597985687917884 1107 90-91 0.5550790804787056 1107 92-93 0.38404550289803296 1107 94-95 0.7757785751210697 1107 96-97 0.7401489948784139 1107 98-99 0.7101059374202343 1107 100-101 0.8990368649092844 1108 1 0.0013188942129787051 1108 2 -0.010038834496207016 1108 3 -0.07842657097935657 1108 4 0.1381053586485308 1108 5 -0.06681286591911118 1108 6 -0.2373679492916807 1108 7 0.0818923372463658 1108 8 0.25343173679863185 1108 9 0.22294607357887486 1108 10-11 0.16445677795770308 1108 12-13 0.0981693171664304 1108 14-15 0.17598738305631656 1108 16-17 0.23832166043758463 1108 18-19 0.0895618422681963 1108 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-0.200042899289528 2315 88-89 -0.044914689524240714 2315 90-91 0.17130413874045658 2315 92-93 -1.18375346215835 2315 94-95 -0.22612878365714195 2315 96-97 -0.18678161822997907 2315 98-99 -2.264869632940485 2315 100-101 -3.6056971122695707 2316 1 -0.4614989925197257 2316 2 -0.45561873219654103 2316 3 -0.8250845901003849 2316 4 -0.2755477205731651 2316 5 -0.14762720164785037 2316 6 -0.4403406430887955 2316 7 -0.6857950819745113 2316 8 -0.9124500517857683 2316 9 -5.1679420973474635 2316 10-11 -1.3625793557628683 2316 12-13 -0.7161135308978359 2316 14-15 -0.35985471613760467 2316 16-17 -0.4550708548139326 2316 18-19 -0.3078620912141403 2316 20-21 -3.4602124843717874 2316 22-23 -1.2527759417985749 2316 24-25 -0.3807276913341866 2316 26-27 -0.4891926248305296 2316 28-29 -0.06959463045186709 2316 30-31 -0.026618644287189852 2316 32-33 -1.3433353269611459 2316 34-35 -0.5217810004525205 2316 36-37 -0.43226052257352165 2316 38-39 -5.659608399865846 2316 40-41 -5.522494755063015 2316 42-43 -3.866653137065491 2316 44-45 -2.383693829892927 2316 46-47 -2.6595520387125653 2316 48-49 -3.107672212940514 2316 50-51 -1.5760511213670227 2316 52-53 -1.2371727566247976 2316 54-55 -0.5448536603808556 2316 56-57 -3.870170074957148 2316 58-59 -1.096166915408407 2316 60-61 -0.8753705561238725 2316 62-63 -0.701259787948743 2316 64-65 -0.36936739220885073 2316 66-67 -0.7048422947089641 2316 68-69 2.001620100762011 2316 70-71 2.7671477873859125 2316 72-73 1.7434207537328668 2316 74-75 0.32239632208808544 2316 76-77 -1.8226460552243147 2316 78-79 -0.38096671214022493 2316 80-81 -0.3965333292320956 2316 82-83 -0.0669391314143617 2316 84-85 0.044965107881587585 2316 86-87 0.04204352294451397 2316 88-89 -0.19498836430742372 2316 90-91 -0.28231574119607217 2316 92-93 0.0635607623891481 2316 94-95 0.1468238879176873 2316 96-97 0.38618298883397273 2316 98-99 0.45395042634782 2316 100-101 0.5644535943346156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 28.0 4 99.0 5 244.0 6 662.0 7 1574.0 8 3205.0 9 5411.0 10 8701.0 11 12597.0 12 15898.0 13 20058.0 14 24801.0 15 31053.0 16 37112.0 17 40408.0 18 35676.0 19 29771.0 20 28568.0 21 28725.0 22 29965.0 23 33334.0 24 39570.0 25 48080.0 26 59679.0 27 73100.0 28 88319.0 29 104486.0 30 120969.0 31 139635.0 32 160503.0 33 183943.0 34 210695.0 35 231957.0 36 227209.0 37 177407.0 38 68530.0 39 3539.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.68654044078275 20.945158705616254 16.059070376170222 21.309230477430777 2 16.0828035750459 21.961819555747088 38.76024303293125 23.195133836275765 3 18.706176279611782 26.76066755249386 30.907929873447117 23.62522629444724 4 12.919778528412202 18.58207567634572 36.45470713206001 32.04343866318207 5 12.778991365372047 38.92247755340741 33.52493205820634 14.773599023014208 6 30.24342942653686 37.66103925143624 17.04079087687915 15.054740445147752 7 27.179426536860575 34.81509511851113 20.478946644191407 17.52653170043689 8 26.973193092297638 34.71000034400908 19.062403247445733 19.254403316247547 9 26.877945577763253 16.765841618218722 20.46381024459046 35.892402559427566 10-11 24.921808289084726 27.712583075668935 26.10333369243999 21.262274942806354 12-13 26.187304344834704 25.50388730262479 27.330059513571157 20.97874883896935 14-15 23.038288210808766 26.288120506381368 26.65902679830748 24.01456448450239 16-17 21.056047920671165 29.6712979088458 27.479671125903565 21.79298304457947 18-19 20.761865323652902 26.83812968820683 31.605093235221776 20.79491175291849 20-21 21.69992087791118 25.98984313185868 31.867754308713753 20.44248168151639 22-23 21.37112024027371 26.0228005458577 31.895737480888638 20.710341732979952 24-25 21.091951946993078 26.59140323174361 31.877410304837984 20.439234516425337 26-27 20.869716287673302 26.677636650151488 31.804394572803858 20.648252489371348 28-29 20.546813319504 26.48714766595628 32.296047460064365 20.669991554475352 30-31 20.69597487008254 26.536014603216923 32.009124860514774 20.75888566618577 32-33 20.62726980122395 26.7101166993083 31.714596368576046 20.9480171308917 34-35 21.20864386583018 26.731918805938314 31.427092753589537 20.63234457464197 36-37 21.458332167722315 26.316627581039377 31.36321400489169 20.861826246346617 38-39 20.929464278027908 26.681932834600254 31.483544390974593 20.905058496397242 40-41 20.92819789941453 26.459238997704293 31.85878639772895 20.753776705152223 42-43 21.090064322436763 26.6550353201016 31.11056693776828 21.144333419693357 44-45 20.996059392505636 26.934104943285853 30.987854545470196 21.081981118738316 46-47 21.40538451574088 27.312975246410513 30.017476716718388 21.264163521130218 48-49 21.4072652856245 27.482634409978758 29.67957546691775 21.43052483747899 50-51 21.509243317051002 27.597731633751287 29.42774015002236 21.465284899175355 52-53 21.744704103123432 27.6203906281933 29.348250261917514 21.286655006765752 54-55 21.304145156741825 27.6124515818305 29.432181005815885 21.651222255611795 56-57 21.396053942895684 28.1289154859364 28.97929505015908 21.495735521008832 58-59 21.417275117073657 27.54181447825234 29.241425494358786 21.79948491031521 60-61 21.300771749952155 27.808372057017895 29.530585080647764 21.36027111238219 62-63 21.368665145115358 27.742345389280267 29.590632461505216 21.29835700409916 64-65 21.127253289572707 27.583343439217195 29.434139309824037 21.85526396138606 66-67 20.990410932355406 27.876257313467544 29.332167628875304 21.801164125301746 68-69 21.33883729108955 27.341477527671376 29.80182200578288 21.517863175456196 70-71 20.92680410850635 27.43060887550504 29.995171923760644 21.647415092227966 72-73 21.203401423661084 27.751940326203524 29.01856283161399 22.026095418521404 74-75 21.112676232070246 28.182201878518025 28.733620189314625 21.971501700097104 76-77 20.59256530562126 28.237319098151236 29.32124376887648 21.848871827351026 78-79 21.11492701413485 28.118077911655742 28.77812567937397 21.988869394835437 80-81 21.02089795363722 28.723494835177643 28.41785414650026 21.83775306468488 82-83 21.291891593203385 28.581779730348316 28.172853664929175 21.953475011519117 84-85 20.976901890548973 28.393022620795822 28.15552684029129 22.47454864836391 86-87 21.13800948319735 28.290141203015374 28.35253688322182 22.219312430565456 88-89 21.086040173940155 28.395052703436342 28.12165060389914 22.39725651872437 90-91 20.986479707735835 28.623624743891508 28.23199833321185 22.157897215160805 92-93 21.03570315103691 28.317017319414727 28.268402550071492 22.37887697947687 94-95 20.681634404307943 28.019214109856268 28.41018389135645 22.888967594479343 96-97 20.948999590475633 27.787884445300964 28.31050120276274 22.95261476146066 98-99 20.772848796165345 27.90369322757396 28.0190158718622 23.304442104398497 100-101 20.60952782344207 27.762695718833623 28.394178241624264 23.23359821610004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6424.0 1 4326.5 2 2267.5 3 2332.5 4 2169.5 5 2075.5 6 3052.0 7 5578.0 8 8129.5 9 9295.0 10 8386.0 11 6605.5 12 5801.0 13 5618.5 14 5676.0 15 5864.5 16 6024.5 17 6231.5 18 6599.5 19 7039.0 20 7663.5 21 8643.5 22 9905.0 23 11126.5 24 12481.5 25 14354.0 26 17191.5 27 20522.5 28 23318.0 29 26519.0 30 30630.0 31 35233.5 32 41592.0 33 47705.0 34 52108.0 35 56505.0 36 63156.0 37 68938.0 38 70831.5 39 70794.0 40 69305.5 41 69442.5 42 70288.0 43 71058.0 44 72272.0 45 74036.0 46 74228.5 47 73437.0 48 76565.5 49 75988.5 50 71311.0 51 68087.0 52 66111.5 53 66515.5 54 66672.5 55 63623.5 56 59188.5 57 54742.5 58 50374.0 59 45194.0 60 38851.0 61 33111.0 62 28054.5 63 22628.5 64 17564.5 65 13650.5 66 10293.5 67 7954.0 68 5551.0 69 3445.0 70 2476.0 71 1928.0 72 1575.0 73 1284.5 74 1015.5 75 813.0 76 555.0 77 324.0 78 189.5 79 108.5 80 71.0 81 49.5 82 34.5 83 19.5 84 8.5 85 4.5 86 6.0 87 6.5 88 2.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01844748701365716 2 0.017329457497677937 3 4.300113522997007E-4 4 0.0925384430148956 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013136846812755856 12-13 0.0 14-15 0.0 16-17 4.300113522997007E-4 18-19 1.7200454091988027E-4 20-21 0.0 22-23 2.3650624376483537E-4 24-25 0.003784099900237366 26-27 0.0035905947917025008 28-29 0.001204031786439162 30-31 2.1500567614985035E-4 32-33 5.80515325604596E-4 34-35 0.0033970896831676356 36-37 0.08552925797241047 38-39 0.09890261102893116 40-41 0.0788855825793801 42-43 0.08230417283016271 44-45 0.08142264955794833 46-47 0.07985310812205441 48-49 0.07497247927345282 50-51 0.07452096735353814 52-53 0.056546492827410644 54-55 0.04766675840242183 56-57 0.02653170043689153 58-59 0.020984553992225395 60-61 0.012255323540541471 62-63 0.001827548247273728 64-65 0.00610616120265575 66-67 0.1550190925040421 68-69 0.7153668856857821 70-71 0.8709449929478138 72-73 0.3549743713234029 74-75 0.05336440882039285 76-77 0.19393511988716503 78-79 0.013308851353675738 80-81 0.0033110874127076956 82-83 0.0016555437063538478 84-85 2.1500567614985035E-4 86-87 0.0016555437063538478 88-89 3.2250851422477555E-4 90-91 0.003848601603082321 92-93 0.004085107846847157 94-95 0.07787505590147581 96-97 0.0374969899205339 98-99 0.013093845677525888 100-101 0.011932815026316695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2325520.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.71837541112339 #Duplication Level Percentage of deduplicated Percentage of total 1 69.78048487437664 27.715674946107093 2 14.180062520568892 11.264180930903118 3 5.885312998753052 7.012652132893142 4 2.998968227918044 4.764565836899213 5 1.7909311074797163 3.556643703116917 6 1.1154520511322679 2.658236599198741 7 0.7629042824041921 2.1210923083898376 8 0.5559765584358994 1.7665988534193149 9 0.4102938924130009 1.4666586162975577 >10 2.1674171751831497 15.719307287065543 >50 0.2059435737800714 5.72465246638627 >100 0.1351073346264506 9.823538381129966 >500 0.008055588255335831 2.141199408016905 >1k 0.00264841257709671 1.7170619615677687 >5k 1.1035052404569624E-4 0.35536906229302306 >10k+ 3.3105157213708875E-4 2.1925675063157963 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19530 0.8398121710413156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17903 0.7698493240221542 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12592 0.5414702948157831 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8108 0.34865320444459735 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4579 0.19690219821803298 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3617 0.15553510612680174 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2558 0.10999690391826344 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.300113522997007E-5 0.0 0.0 0.0 0.0 3 4.300113522997007E-5 0.0 0.0 0.0 0.0 4 4.300113522997007E-5 0.0 0.0 0.0 0.0 5 4.300113522997007E-5 0.0 0.0 0.0 0.0 6 4.300113522997007E-5 0.0 0.0 0.0 0.0 7 4.300113522997007E-5 0.0 0.0 0.0 0.0 8 4.300113522997007E-5 0.0 0.0 0.0 0.0 9 4.300113522997007E-5 0.0 0.0 0.0 0.0 10-11 4.300113522997007E-5 0.0 0.0 0.0 0.0 12-13 4.300113522997007E-5 0.0 0.0 4.300113522997007E-5 0.0 14-15 8.600227045994014E-5 0.0 0.0 8.600227045994014E-5 0.0 16-17 1.0750283807492517E-4 0.0 0.0 1.5050397330489525E-4 0.0 18-19 1.2900340568991023E-4 0.0 0.0 2.365062437648354E-4 0.0 20-21 1.2900340568991023E-4 0.0 0.0 4.085107846847157E-4 0.0 22-23 1.2900340568991023E-4 0.0 0.0 0.0010320272455192816 0.0 24-25 1.7200454091988027E-4 0.0 0.0 0.001870549382503698 0.0 26-27 1.7200454091988027E-4 0.0 0.0 0.00285957549279301 0.0 28-29 2.1500567614985035E-4 0.0 0.0 0.0046871237400667376 0.0 30-31 2.1500567614985035E-4 0.0 0.0 0.010083766211427982 0.0 32-33 2.1500567614985035E-4 0.0 0.0 0.01947951425917644 0.0 34-35 2.795073789948055E-4 0.0 0.0 0.03278836561285218 0.0 36-37 3.010079466097905E-4 0.0 0.0 0.05491244968867178 0.0 38-39 3.225085142247755E-4 0.0 0.0 0.08802332381574873 0.0 40-41 3.4400908183976055E-4 0.0 0.0 0.12524080635728785 0.0 42-43 3.655096494547456E-4 0.0 0.0 0.16155526505899756 0.0 44-45 4.730124875296708E-4 0.0 0.0 0.20627644569816644 0.0 46-47 4.730124875296708E-4 0.0 0.0 0.26525250266607037 0.0 48-49 4.730124875296708E-4 0.0 0.0 0.32566909766417834 0.0 50-51 4.730124875296708E-4 0.0 0.0 0.39343888678661115 0.0 52-53 4.945130551446558E-4 0.0 0.0 0.46387474629330216 0.0 54-55 5.590147579896109E-4 0.0 0.0 0.5376646943479308 0.0 56-57 5.590147579896109E-4 0.0 0.0 0.6218394165605972 0.0 58-59 5.590147579896109E-4 2.1500567614985034E-5 0.0 0.7100132443496509 2.1500567614985034E-5 60-61 5.590147579896109E-4 4.300113522997007E-5 0.0 0.8044437373146651 4.300113522997007E-5 62-63 5.590147579896109E-4 4.300113522997007E-5 0.0 0.8899944958546906 4.300113522997007E-5 64-65 5.590147579896109E-4 1.7200454091988027E-4 0.0 0.9883165915580171 4.300113522997007E-5 66-67 5.590147579896109E-4 1.7200454091988027E-4 0.0 1.0907452956758057 4.300113522997007E-5 68-69 5.590147579896109E-4 1.7200454091988027E-4 0.0 1.2079233891774743 4.300113522997007E-5 70-71 5.590147579896109E-4 1.7200454091988027E-4 0.0 1.3610719322990126 4.300113522997007E-5 72-73 5.80515325604596E-4 1.7200454091988027E-4 0.0 1.528690357425436 4.300113522997007E-5 74-75 6.02015893219581E-4 1.7200454091988027E-4 0.0 1.7075535794144965 4.300113522997007E-5 76-77 6.02015893219581E-4 1.7200454091988027E-4 0.0 1.9119594757301592 4.300113522997007E-5 78-79 6.02015893219581E-4 1.7200454091988027E-4 0.0 2.1415425367229695 4.300113522997007E-5 80-81 0.0010105266779042968 1.7200454091988027E-4 0.0 2.40606402009013 4.300113522997007E-5 82-83 0.0010320272455192818 1.7200454091988027E-4 0.0 2.67899222539475 4.300113522997007E-5 84-85 0.0010320272455192818 1.7200454091988027E-4 0.0 2.947964326258213 4.300113522997007E-5 86-87 0.0010535278131342668 1.7200454091988027E-4 0.0 3.2273857029825583 4.300113522997007E-5 88-89 0.0010750283807492518 1.7200454091988027E-4 0.0 3.538520416939007 4.300113522997007E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10495 0.0 46.765965 1 GTATCAA 17380 0.0 36.324005 1 TATCAAC 22605 0.0 27.793575 2 ATCAACG 22955 0.0 27.427094 3 TCAACGC 23215 0.0 27.128092 4 CAACGCA 23530 0.0 26.700447 5 AACGCAG 24130 0.0 26.01688 6 ACGCAGA 27040 0.0 23.111774 7 TAAGGTA 495 0.0 23.010342 4 CGCAGAG 27160 0.0 22.9922 8 GTACATG 15320 0.0 22.927927 1 GAGTACT 15915 0.0 22.59138 12-13 TAAGGTG 1185 0.0 22.007406 5 TACATGG 15790 0.0 21.848076 2 GCAGAGT 27845 0.0 21.558128 9 AGTACTT 16795 0.0 21.548786 12-13 GTACTTT 17040 0.0 21.211142 14-15 ACATGGG 15540 0.0 20.82186 3 GGTAAGG 1085 0.0 20.55201 3 GTAAGGT 1205 0.0 20.086311 4 >>END_MODULE