FastQCFastQC Report
Fri 27 May 2016
SRR939545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151332
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110670.5144254815156377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87420.40635290136529373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59850.27819973858056307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29150.13549744995193677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65850.055.25911
GTATCAA121900.044.4281351
TATCAAC165550.032.643222
ATCAACG163900.032.5747533
TCAACGC166450.032.1694764
CAACGCA166550.031.6711335
AACGCAG171000.030.5634886
GTACATG119100.027.2835771
TACATGG122550.026.7206232
ACGCAGA189600.026.6337247
CGCAGAG189300.026.5675058
GCAGAGT188950.025.0127359
ACATGGG127050.024.7991313
GAGTACT111850.023.7800512-13
TGGTATC31900.023.3717582
GTATCGA1502.0686311E-523.293721
GTGGTAT32200.023.2523841
AGTACTT116150.022.4874512-13
AGAGTAC173200.022.297210-11
CATGGGG46150.021.8780424
Produced by FastQC (version 0.11.5)