FastQCFastQC Report
Fri 27 May 2016
SRR939545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151332
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154690.7190428999336226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140310.6522005901460118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96410.44814096569009343No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45850.21312377633949572No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.16603666937506623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.12457398486147186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72250.057.0155221
GTATCAA127500.040.004911
ATCAACG172850.029.3314633
TATCAAC175500.029.0019552
TCAACGC176500.028.7248944
CAACGCA180000.028.0347335
TGGTATC31250.027.7524412
AACGCAG183800.027.5060446
GTGGTAT32150.027.2773321
GTACATG134100.024.4618031
TACATGG136100.023.8176062
ACGCAGA212000.023.6460747
CGCAGAG213350.023.340998
TAAGGTG8800.023.1528875
ACATGGG139350.022.2037093
GAGTACT131550.021.93541112-13
GCAGAGT219500.021.8883329
AGTACTT133500.021.72148512-13
GTACTTT140000.020.57760814-15
AGAGTAC205200.020.52738610-11
Produced by FastQC (version 0.11.5)