FastQCFastQC Report
Fri 27 May 2016
SRR939544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128657
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109610.5149256080242143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81330.382071888519381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59430.2791901184643651No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28840.13548448622770132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.058.0611651
GTATCAA118500.045.7006151
TATCAAC160450.033.1163982
ATCAACG162900.033.040323
TCAACGC166400.032.804084
CAACGCA167300.032.1009375
CAACGCG3200.031.80855
AACGCAG171050.031.0898156
GTATCGA1603.693276E-828.2058491
GTACATG118950.027.8224981
ACGCAGA190800.027.3379367
CGCAGAG192500.026.9739638
AACGCGG4250.026.2106486
TACATGG122100.026.1186962
TGGTATC27100.025.5901622
GCAGAGT187200.025.3824429
ACATGGG125700.024.7190883
GTGGTAT27900.024.4428231
GAGTACT109550.023.80728512-13
CATGGGG43400.022.1889824
Produced by FastQC (version 0.11.5)