FastQCFastQC Report
Fri 27 May 2016
SRR939544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128657
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151750.7128908039200303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136450.6410144988131015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97770.4593036830264341No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44550.20928688839958717No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34930.16409407433889067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26090.12256554249933174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74600.055.2831341
GTATCAA131550.040.2971231
ATCAACG175200.030.0258063
TCAACGC177750.029.5760154
TATCAAC178350.029.5570262
GTGGTAT32900.028.9942471
CAACGCA182300.028.706725
TGGTATC33950.028.5108472
AACGCAG188400.027.7772586
GTACATG135200.025.0279061
TACATGG135400.024.5651322
ACGCAGA216400.024.095547
CGCAGAG217800.023.9406558
ACATGGG139350.022.7727973
GAGTACT131800.022.59899112-13
GCAGAGT224000.022.4315389
AGTACTT136600.021.18039912-13
GTACTTT142150.020.85353314-15
CATGGGG47650.020.8109994
GGACCGT2309.227551E-820.6097816
Produced by FastQC (version 0.11.5)