##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939544_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2128657 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84155361807938 31.0 31.0 31.0 28.0 34.0 2 31.09022966123711 31.0 31.0 34.0 28.0 34.0 3 31.072228639935883 31.0 31.0 34.0 27.0 34.0 4 35.00153758919356 35.0 35.0 37.0 32.0 37.0 5 34.570417404025164 35.0 35.0 37.0 30.0 37.0 6 34.64922624922662 35.0 35.0 37.0 30.0 37.0 7 34.482593015220395 35.0 35.0 37.0 30.0 37.0 8 34.38308097546951 35.0 35.0 37.0 30.0 37.0 9 35.86206232380322 37.0 35.0 39.0 30.0 39.0 10-11 35.64930164888002 37.0 35.0 39.0 30.0 39.0 12-13 35.6308987309839 37.0 35.0 39.0 30.0 39.0 14-15 36.40193018414897 38.0 35.0 40.0 30.0 41.0 16-17 36.32566566619235 38.0 34.5 40.0 30.0 41.0 18-19 36.37296638208974 38.0 35.0 40.0 30.0 41.0 20-21 36.27576096101909 38.0 34.0 40.0 29.5 41.0 22-23 36.113012570836915 38.0 34.0 40.0 29.0 41.0 24-25 35.93182438504654 38.0 34.0 40.0 29.0 41.0 26-27 35.45330130688035 38.0 34.0 40.0 27.0 41.0 28-29 35.22517977297423 38.0 33.5 40.0 27.0 41.0 30-31 34.874508903970906 37.0 33.0 40.0 26.5 41.0 32-33 34.556830903240865 37.0 33.0 40.0 25.0 41.0 34-35 34.72834256528882 37.0 33.0 40.0 26.0 41.0 36-37 34.522319236964904 37.0 33.0 40.0 25.0 41.0 38-39 34.30882429625816 37.0 33.0 40.0 24.0 41.0 40-41 34.11148343767925 37.0 33.0 40.0 24.0 41.0 42-43 33.877681326770826 37.0 33.0 40.0 24.0 41.0 44-45 33.417995477899915 37.0 32.0 40.0 22.5 41.0 46-47 32.91069016755635 37.0 31.0 39.5 19.0 41.0 48-49 32.4456039653171 36.0 31.0 39.0 16.5 40.5 50-51 32.16637602958109 36.0 30.0 39.0 15.0 40.0 52-53 32.03576081068955 36.0 30.0 39.0 14.5 40.0 54-55 31.6810087299175 35.5 30.0 39.0 11.0 40.0 56-57 31.175808737621892 35.0 29.0 39.0 8.5 40.0 58-59 30.723375583760088 35.0 28.0 38.0 8.0 40.0 60-61 30.35847132722651 34.0 28.0 38.0 7.0 40.0 62-63 30.185717097681778 34.0 28.0 38.0 6.0 40.0 64-65 29.724764957435603 34.0 28.0 38.0 2.0 40.0 66-67 28.8400397527643 33.5 26.5 37.0 2.0 39.5 68-69 25.41560359419108 31.0 15.5 35.0 2.0 39.0 70-71 24.116033959440152 29.0 10.5 35.0 2.0 38.0 72-73 25.11825860155018 30.0 16.0 35.0 2.0 37.5 74-75 25.857941885423532 31.0 20.5 35.0 2.0 37.0 76-77 25.543668613590633 30.5 21.0 34.0 2.0 36.0 78-79 26.651724538053806 31.5 24.5 35.0 2.0 36.0 80-81 26.670555425322164 32.0 24.5 35.0 2.0 36.0 82-83 26.337014840812778 32.0 24.0 35.0 2.0 36.0 84-85 25.97420039959467 32.0 23.5 34.0 2.0 35.5 86-87 25.522865825729557 31.0 21.0 34.0 2.0 35.0 88-89 25.02377837293655 31.0 20.0 34.0 2.0 35.0 90-91 24.432101085332206 31.0 18.0 34.0 2.0 35.0 92-93 23.866401914446527 30.5 11.5 34.0 2.0 35.0 94-95 23.003108767640818 30.0 4.5 34.0 2.0 35.0 96-97 22.331372315971997 29.5 2.0 34.0 2.0 35.0 98-99 21.471439034095205 29.0 2.0 34.0 2.0 35.0 100-101 20.078764920792782 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28175685535400774 1101 2 0.2715674975574274 1101 3 0.36817622279157547 1101 4 0.29843694682986666 1101 5 0.5994507826080877 1101 6 0.4553417715379169 1101 7 0.6099576064674039 1101 8 0.6921364330984119 1101 9 0.7110242799821904 1101 10-11 0.7717967642862433 1101 12-13 0.7120188715949425 1101 14-15 0.8530821628509031 1101 16-17 0.7282924728143669 1101 18-19 0.5654922914779021 1101 20-21 0.5533998746801885 1101 22-23 0.6933229322826904 1101 24-25 0.547215161081013 1101 26-27 0.690240125169403 1101 28-29 0.7778629269522739 1101 30-31 0.4679617283522788 1101 32-33 0.7495418607831397 1101 34-35 0.7625951994505726 1101 36-37 0.9914969450399553 1101 38-39 1.1337849747380488 1101 40-41 1.1270577714535364 1101 42-43 0.7541744224586395 1101 44-45 0.9002921672269579 1101 46-47 0.985725275276252 1101 48-49 1.0793981452671417 1101 50-51 0.8358150921580147 1101 52-53 0.7838434410774759 1101 54-55 0.925188807786629 1101 56-57 0.8244121517270315 1101 58-59 0.8531524251807987 1101 60-61 0.8854827938263838 1101 62-63 0.5223130724516167 1101 64-65 0.4543116226419919 1101 66-67 0.07276798694489983 1101 68-69 -7.134562625562616 1101 70-71 -7.524204135916573 1101 72-73 -4.659848036037431 1101 74-75 -2.5768194307822583 1101 76-77 0.005888894340639439 1101 78-79 0.11865109889114223 1101 80-81 0.04609682350214683 1101 82-83 -0.3302018633324266 1101 84-85 -0.30270840027268164 1101 86-87 -0.29882152100994475 1101 88-89 -0.0030744840594501 1101 90-91 -0.22543717808102315 1101 92-93 -0.13257838850829984 1101 94-95 -0.2019570958056498 1101 96-97 -0.47306691343865737 1101 98-99 -0.30371073536906223 1101 100-101 -0.008935353515084898 1102 1 0.37794326265726497 1102 2 0.3581896907775928 1102 3 0.2974018481456575 1102 4 0.38599351756991496 1102 5 0.5224400283276154 1102 6 0.39293344889904347 1102 7 0.41947513540654313 1102 8 0.6581240794132484 1102 9 0.6827069066826894 1102 10-11 0.7970079226428268 1102 12-13 0.6565062213208108 1102 14-15 0.7691647732925517 1102 16-17 0.8001406226372367 1102 18-19 0.6184300907759663 1102 20-21 0.6733441797082378 1102 22-23 0.7256002784683844 1102 24-25 0.7211382361747738 1102 26-27 0.7515077145310585 1102 28-29 0.704911655077261 1102 30-31 0.5627001878201128 1102 32-33 0.7915502259918412 1102 34-35 0.6656723373746445 1102 36-37 0.8469766559766043 1102 38-39 0.8959896064507973 1102 40-41 0.6807450123598429 1102 42-43 0.4241468853074366 1102 44-45 0.7049022745943603 1102 46-47 0.8689572635051164 1102 48-49 0.930372351893773 1102 50-51 0.6710414957102628 1102 52-53 0.38450946334972613 1102 54-55 0.12346422348764818 1102 56-57 0.4077245571402841 1102 58-59 0.3712850835135164 1102 60-61 0.5352669677987087 1102 62-63 0.2636748450433579 1102 64-65 0.18341129255710698 1102 66-67 -0.0013264637802627988 1102 68-69 -6.794909229024434 1102 70-71 -7.3864055462194536 1102 72-73 -1.6615226631845381 1102 74-75 0.15556877575430406 1102 76-77 0.8537180098477499 1102 78-79 0.3447166560997559 1102 80-81 0.04170727308109434 1102 82-83 -0.061654343067480966 1102 84-85 -0.19085303853955793 1102 86-87 -0.22420101911428603 1102 88-89 -0.3422001428251171 1102 90-91 -0.3308135424788077 1102 92-93 -0.3343835249198257 1102 94-95 -0.2642977013733585 1102 96-97 -0.3505388184438374 1102 98-99 -0.52240491411278 1102 100-101 -0.4042503272858937 1103 1 0.3162660267905153 1103 2 0.19885406309591858 1103 3 0.2600681509708238 1103 4 0.3206442509167502 1103 5 0.3605191485995505 1103 6 0.47439405783073596 1103 7 0.41210565012644906 1103 8 0.4304502045606 1103 9 0.4681879359360508 1103 10-11 0.5660650675096477 1103 12-13 0.3412781833784706 1103 14-15 0.5187224431589357 1103 16-17 0.5473043962272683 1103 18-19 0.49645087211428773 1103 20-21 0.5651298646356295 1103 22-23 0.6726264250564 1103 24-25 0.5904216520362908 1103 26-27 0.7082999545376367 1103 28-29 0.6380762879885893 1103 30-31 0.4937422801573206 1103 32-33 0.5509353451570007 1103 34-35 0.4752555495922621 1103 36-37 0.7248290476271251 1103 38-39 0.7360071149105352 1103 40-41 0.7404220804301218 1103 42-43 0.547263391140902 1103 44-45 0.6883969495988751 1103 46-47 0.8300930591527518 1103 48-49 0.9990922936565312 1103 50-51 0.732684409572208 1103 52-53 0.4220187535960953 1103 54-55 0.4299981162555362 1103 56-57 0.7340355517919832 1103 58-59 0.93589070672909 1103 60-61 0.6291023120060437 1103 62-63 0.3266908085891522 1103 64-65 0.2716850313081771 1103 66-67 0.13795672326788733 1103 68-69 -6.484566695416412 1103 70-71 -7.56837539200772 1103 72-73 -1.377108327942448 1103 74-75 0.24424353111468022 1103 76-77 0.5446547971894553 1103 78-79 0.3736319705783018 1103 80-81 -0.017894117087216443 1103 82-83 0.10628492139077039 1103 84-85 -0.1769331350818497 1103 86-87 -0.08756782982038303 1103 88-89 0.05889646641504598 1103 90-91 -0.1474755622567585 1103 92-93 -0.3142978639754723 1103 94-95 -0.12650561475648203 1103 96-97 -0.005739280138364222 1103 98-99 0.02495538473442238 1103 100-101 0.20310814105438624 1104 1 0.2715779889660901 1104 2 0.3199844202551816 1104 3 0.27636782705490504 1104 4 0.2361861190740413 1104 5 0.42368767923711914 1104 6 0.37547503012962125 1104 7 0.4610228824699405 1104 8 0.48603881882007727 1104 9 0.5126942668680599 1104 10-11 0.4743004981825436 1104 12-13 0.36889303825094544 1104 14-15 0.6630317022774719 1104 16-17 0.648059030963843 1104 18-19 0.6460599434300676 1104 20-21 0.5861113323697111 1104 22-23 0.4724591645299654 1104 24-25 0.4926369563327029 1104 26-27 0.6969600582675497 1104 28-29 0.5698029293421101 1104 30-31 0.6518675259751774 1104 32-33 0.6857382274157757 1104 34-35 0.5459103874965194 1104 36-37 0.7699989093344826 1104 38-39 0.6978716313789732 1104 40-41 0.8342387017674824 1104 42-43 0.7791147889729473 1104 44-45 0.7349667727269207 1104 46-47 0.8803834478100967 1104 48-49 0.8409047531044109 1104 50-51 0.634970526113463 1104 52-53 0.4617788270136991 1104 54-55 0.4344565320152718 1104 56-57 0.41187332327719517 1104 58-59 0.40155921022753205 1104 60-61 0.594017349837987 1104 62-63 0.3483969327627676 1104 64-65 0.20367955672000448 1104 66-67 0.1178128942460539 1104 68-69 -6.246291006980268 1104 70-71 -3.990630728603872 1104 72-73 0.1968127247550555 1104 74-75 0.8478294865747777 1104 76-77 0.9957648709828284 1104 78-79 0.4328526350610318 1104 80-81 0.2615575556433072 1104 82-83 0.1182140289811251 1104 84-85 0.18274797600235715 1104 86-87 0.1158174631110036 1104 88-89 0.36622227706076416 1104 90-91 0.1812986761149631 1104 92-93 0.15838823319262474 1104 94-95 0.1562273871733204 1104 96-97 0.0971068209711703 1104 98-99 0.4594848716045128 1104 100-101 0.45006183669367417 1105 1 0.19717306953955216 1105 2 0.21483057260679317 1105 3 0.2205368782423207 1105 4 0.16246841729869743 1105 5 0.4284879858135682 1105 6 0.27561408157831835 1105 7 0.2484934123393714 1105 8 0.36457213589557114 1105 9 0.4898606522606599 1105 10-11 0.5036344581574355 1105 12-13 0.37249670175916805 1105 14-15 0.32421229630954684 1105 16-17 0.3976392135027851 1105 18-19 0.3801304962123382 1105 20-21 0.4363689845541714 1105 22-23 0.47920555492050454 1105 24-25 0.5395767860139387 1105 26-27 0.6427743785233773 1105 28-29 0.6484618817966279 1105 30-31 0.5587338737826855 1105 32-33 0.573178240332723 1105 34-35 0.6020285842427668 1105 36-37 0.7873717983552808 1105 38-39 0.8111705909887306 1105 40-41 0.801748866827019 1105 42-43 0.7172188052182804 1105 44-45 0.7243128892353567 1105 46-47 0.7955713598810732 1105 48-49 0.7594039722590082 1105 50-51 0.507120658130269 1105 52-53 0.49796620445178164 1105 54-55 0.7579902443707134 1105 56-57 0.7246292921720112 1105 58-59 0.9189949085632705 1105 60-61 0.8651490832477862 1105 62-63 0.559313208448426 1105 64-65 0.5440041251111403 1105 66-67 0.4091373199344268 1105 68-69 -2.551536070503097 1105 70-71 -3.0254254141653654 1105 72-73 0.6114984972895279 1105 74-75 1.024085907361684 1105 76-77 1.1458876757554002 1105 78-79 0.562953337654406 1105 80-81 0.4769713132616573 1105 82-83 0.4457303543782629 1105 84-85 0.4617065037654129 1105 86-87 0.38919852370742447 1105 88-89 0.6116490889671304 1105 90-91 0.6985363994900098 1105 92-93 0.486526789536434 1105 94-95 0.6623778427837159 1105 96-97 0.8933839705069175 1105 98-99 0.7092882365758761 1105 100-101 0.8080845626112421 1106 1 0.0796681413250866 1106 2 0.12284297772014696 1106 3 0.16499705530664244 1106 4 0.15303711361113415 1106 5 0.3830588401361723 1106 6 0.31976285612206823 1106 7 0.34231879541718513 1106 8 0.48052573592092784 1106 9 0.36119980367759297 1106 10-11 0.33343156937281293 1106 12-13 0.2108268931619861 1106 14-15 0.32173923181797903 1106 16-17 0.1846031474478309 1106 18-19 0.3714142228726516 1106 20-21 0.2532291452198123 1106 22-23 0.2657411769021749 1106 24-25 0.31089971101344105 1106 26-27 0.6104491845815048 1106 28-29 0.5108440335642754 1106 30-31 0.35397302223682914 1106 32-33 0.6916865537017003 1106 34-35 0.47318927951891965 1106 36-37 0.6327616009993235 1106 38-39 0.6535469653249493 1106 40-41 0.49881147185948294 1106 42-43 0.4080016833506548 1106 44-45 0.5320350964139777 1106 46-47 0.633640757271209 1106 48-49 0.6698586943531879 1106 50-51 0.5635127124911747 1106 52-53 0.46910712485827943 1106 54-55 0.5492581408000099 1106 56-57 0.7078725878366292 1106 58-59 0.8009494442775775 1106 60-61 0.7624505025597692 1106 62-63 0.4628870338246145 1106 64-65 0.5803062554916103 1106 66-67 0.6841278114605167 1106 68-69 -2.846069490377321 1106 70-71 -2.9159925257321504 1106 72-73 1.000279755536237 1106 74-75 1.5278141076349456 1106 76-77 1.5626335797046522 1106 78-79 1.1895834724886996 1106 80-81 0.9529766276717488 1106 82-83 1.0405404329471963 1106 84-85 0.9414960874389493 1106 86-87 0.9418223285163663 1106 88-89 0.8476978150727881 1106 90-91 0.8870975331205848 1106 92-93 0.8387283285311646 1106 94-95 0.8817095693227621 1106 96-97 0.882181799680982 1106 98-99 0.9353423852677345 1106 100-101 0.8968310942575783 1107 1 -0.05624782998925326 1107 2 6.942147307320568E-4 1107 3 0.09458714840049964 1107 4 -0.22178118812059466 1107 5 0.2104295821085458 1107 6 0.08873259403785028 1107 7 0.048792372028778175 1107 8 0.09522688065657547 1107 9 0.16313918591810506 1107 10-11 0.024670002102780586 1107 12-13 0.17837163407561007 1107 14-15 0.05098495733880526 1107 16-17 0.028017535757136613 1107 18-19 0.2552169007468521 1107 20-21 0.13822437792521214 1107 22-23 0.20337671942322544 1107 24-25 0.1691025835277955 1107 26-27 0.420923175772252 1107 28-29 0.13962377797293613 1107 30-31 0.2287909583784824 1107 32-33 0.01646014376701288 1107 34-35 -0.02150865899176324 1107 36-37 0.14984206079465423 1107 38-39 0.23051555821260905 1107 40-41 0.03519051784486038 1107 42-43 0.12536070450515524 1107 44-45 0.3535114295747448 1107 46-47 0.6184705056282453 1107 48-49 0.5663002781771453 1107 50-51 0.6502785710575338 1107 52-53 0.30768685859171896 1107 54-55 0.26551499926985045 1107 56-57 0.7339890633728032 1107 58-59 0.8183282255757476 1107 60-61 0.8253666354649276 1107 62-63 0.47527075790062057 1107 64-65 0.42553378778783824 1107 66-67 0.505070474506379 1107 68-69 -2.4380782762380555 1107 70-71 -2.3824341899859185 1107 72-73 1.3331560098757897 1107 74-75 1.3629511710107174 1107 76-77 0.9435455368399488 1107 78-79 0.787800617639661 1107 80-81 0.592769590355239 1107 82-83 0.592580261061368 1107 84-85 0.5070519634951403 1107 86-87 0.409094247835025 1107 88-89 0.4228609413651476 1107 90-91 0.5022000957607915 1107 92-93 0.6087560841298121 1107 94-95 0.6650545832590566 1107 96-97 0.5685598037346438 1107 98-99 0.7787855637956085 1107 100-101 0.9979236053642069 1108 1 0.03159572358010365 1108 2 0.13785805350747538 1108 3 0.08336792951999072 1108 4 0.15510805167186703 1108 5 -0.03990459766112053 1108 6 -0.010655594060011708 1108 7 0.1570102221821088 1108 8 0.2624825217025375 1108 9 0.2640287061624633 1108 10-11 0.30255191204114595 1108 12-13 0.11410775932787232 1108 14-15 0.30900393814003735 1108 16-17 0.36119305139232694 1108 18-19 0.28580520956096933 1108 20-21 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1.4490097072635777 2311 82-83 1.3234216959711311 2311 84-85 1.2479333721523425 2311 86-87 1.3197849224183962 2311 88-89 1.441059263350514 2311 90-91 1.7071384751640437 2311 92-93 1.758391849023532 2311 94-95 1.9096978036500794 2311 96-97 1.8446353335835006 2311 98-99 1.8671523972914237 2311 100-101 2.184496465818789 2312 1 -0.13390544724111209 2312 2 -0.08317610343278048 2312 3 0.046234359537237424 2312 4 0.02998842097886012 2312 5 -1.353493224288087 2312 6 -0.1992245041286722 2312 7 -0.03228623522784346 2312 8 0.18632183137194858 2312 9 -2.4970002674150678 2312 10-11 -0.07910803704829306 2312 12-13 -1.1678823882255287 2312 14-15 -4.061829021149265 2312 16-17 -0.9113208490982672 2312 18-19 -0.06861393682743966 2312 20-21 0.058515183687219974 2312 22-23 0.1330997196632424 2312 24-25 0.22790560060960985 2312 26-27 0.3347184228463007 2312 28-29 0.2500861595413397 2312 30-31 -0.10132911061593575 2312 32-33 0.3479615757265577 2312 34-35 0.16276727086663811 2312 36-37 0.32420741693357513 2312 38-39 0.4154964077876997 2312 40-41 -0.10725271736669839 2312 42-43 -0.21795312244741183 2312 44-45 0.21615340510939518 2312 46-47 0.3511452443566512 2312 48-49 0.5266263286537125 2312 50-51 0.7203555999042024 2312 52-53 0.5058045131884263 2312 54-55 0.5854256187550781 2312 56-57 0.6479146157145834 2312 58-59 0.6753599554805128 2312 60-61 0.4953066975843079 2312 62-63 0.6464320028511317 2312 64-65 0.8276630902770883 2312 66-67 1.4296018586254569 2312 68-69 3.9391617030961648 2312 70-71 4.695424415195983 2312 72-73 2.7315144686072834 2312 74-75 2.109523951778524 2312 76-77 1.8865142900181233 2312 78-79 1.292176967729361 2312 80-81 1.166250955557807 2312 82-83 1.1024815545032354 2312 84-85 1.2587273714205445 2312 86-87 1.3582074346772686 2312 88-89 1.2837523768639123 2312 90-91 1.3844618494230687 2312 92-93 1.583900195399579 2312 94-95 1.5366182108763446 2312 96-97 1.9194262511325064 2312 98-99 2.0976785309215593 2312 100-101 1.9913197343951516 2313 1 -0.29072918197060815 2313 2 -0.19422031518379512 2313 3 -0.1532275369141871 2313 4 0.05664927030986178 2313 5 0.03988373906270226 2313 6 -0.01457354426828772 2313 7 -2.9414718082354057 2313 8 -3.247366359436665 2313 9 -1.0356564628775473 2313 10-11 -0.26320198701920816 2313 12-13 -0.11073870003472308 2313 14-15 -0.46864693854309536 2313 16-17 -0.35926587527627163 2313 18-19 0.17070252623246773 2313 20-21 -2.448199475998642 2313 22-23 -0.6317330319365269 2313 24-25 0.05628692696213733 2313 26-27 -1.476344695245622 2313 28-29 -1.6337649166657613 2313 30-31 -0.8369530198654971 2313 32-33 -0.8487748989680313 2313 34-35 -1.4966786279117272 2313 36-37 -2.3632533160507236 2313 38-39 -1.9915687463716978 2313 40-41 -1.031172147267803 2313 42-43 -0.05512880598435288 2313 44-45 -0.02485881129549483 2313 46-47 -0.04084864744090311 2313 48-49 -2.04492835430149 2313 50-51 -0.6050283442493765 2313 52-53 -0.4592303908255353 2313 54-55 -0.1573667023618519 2313 56-57 -0.38278055589681514 2313 58-59 -0.231934983437462 2313 60-61 -0.27536665901837054 2313 62-63 0.008164114543976808 2313 64-65 0.19034633635038745 2313 66-67 0.8134980191839212 2313 68-69 1.435152104492328 2313 70-71 3.9627688014670674 2313 72-73 3.0178306408004225 2313 74-75 1.7060989954085422 2313 76-77 1.6426966634910762 2313 78-79 0.9257269968160386 2313 80-81 0.930369629205277 2313 82-83 0.901021403252507 2313 84-85 0.8409495936427724 2313 86-87 0.9503947528855292 2313 88-89 0.7708757043799075 2313 90-91 1.101240499801193 2313 92-93 1.1929985083722343 2313 94-95 1.4232354301899015 2313 96-97 1.4794786071534496 2313 98-99 1.6105668380768812 2313 100-101 1.433349984540584 2314 1 -0.23934315677014695 2314 2 -0.18529133465687764 2314 3 -0.11642914057970799 2314 4 0.008413302383175392 2314 5 -0.16275658167040064 2314 6 -2.5195048157459468 2314 7 -0.7786653935733199 2314 8 0.006551166336528524 2314 9 -0.21303332427502397 2314 10-11 -0.1559253547168069 2314 12-13 -2.47231945604846 2314 14-15 -0.7625301000551232 2314 16-17 -1.676078590052903 2314 18-19 -0.4195552057262901 2314 20-21 -0.7015242182666839 2314 22-23 -0.5168046241472979 2314 24-25 -0.4032348370699381 2314 26-27 -2.014921442095563 2314 28-29 -0.9286904607665605 2314 30-31 -0.20320445170970913 2314 32-33 -0.030355654981775615 2314 34-35 -0.14861161760244812 2314 36-37 -0.6548741637025017 2314 38-39 -0.34275431338200235 2314 40-41 -0.2994167062597839 2314 42-43 -0.3700944417231824 2314 44-45 -3.546508295831572 2314 46-47 -3.8230794607284224 2314 48-49 -3.398688377210995 2314 50-51 -3.819581608854186 2314 52-53 -1.149133025418891 2314 54-55 -1.5349497493263407 2314 56-57 -1.1929122855646241 2314 58-59 -1.822851363735289 2314 60-61 -1.210346613199352 2314 62-63 -1.1868788407824837 2314 64-65 -1.1808650038557964 2314 66-67 -1.0925414997164218 2314 68-69 -1.5327201750036643 2314 70-71 1.3426599813067064 2314 72-73 1.1763157496205814 2314 74-75 0.8898475635643273 2314 76-77 0.7626365260340293 2314 78-79 -1.5703869779835031 2314 80-81 -0.9755181142082421 2314 82-83 0.03993320164884295 2314 84-85 0.2601592156358983 2314 86-87 0.015125566173043836 2314 88-89 -0.07557904934288473 2314 90-91 0.19769238983555937 2314 92-93 -0.23704731064265516 2314 94-95 0.3288883511520986 2314 96-97 0.5672105659163336 2314 98-99 0.4173995986725316 2314 100-101 0.29108194330347104 2315 1 -0.43220622787879037 2315 2 -0.4288543803867242 2315 3 -0.6282275369141885 2315 4 -0.3344418807214069 2315 5 -0.4174382835587238 2315 6 -0.298908208273609 2315 7 -1.2751178494862359 2315 8 -3.130819453249039 2315 9 -1.0239132825714918 2315 10-11 -1.7843081081103591 2315 12-13 -0.7465404299083005 2315 14-15 -0.609039486779956 2315 16-17 -0.44855562910195346 2315 18-19 -0.17350572393386443 2315 20-21 -0.5548045990858057 2315 22-23 -0.22128226680013796 2315 24-25 -5.096898369112381 2315 26-27 -1.0200589334359051 2315 28-29 -0.9493138188613628 2315 30-31 -1.2822041842034793 2315 32-33 -0.2322213394204624 2315 34-35 -2.3202731056229737 2315 36-37 -3.7055254384725487 2315 38-39 -1.3532021462386297 2315 40-41 -0.5073880487980702 2315 42-43 -1.9658174287388412 2315 44-45 -5.68337511202736 2315 46-47 -3.9555608896232073 2315 48-49 -3.0376811819794654 2315 50-51 -1.614545975653236 2315 52-53 -0.74514855449819 2315 54-55 -1.6653706943778168 2315 56-57 -1.5482462910930863 2315 58-59 -1.0518418363982107 2315 60-61 -1.8599059138420522 2315 62-63 -0.9620604363542249 2315 64-65 -0.33546371022313437 2315 66-67 -0.03101129552000259 2315 68-69 2.609851039954737 2315 70-71 3.0774028666699955 2315 72-73 -2.4040040232049016 2315 74-75 -2.049098942049877 2315 76-77 -0.4956383331822458 2315 78-79 -0.350209463596471 2315 80-81 -0.5294041565565308 2315 82-83 -1.1648668203003858 2315 84-85 -0.7878794150066 2315 86-87 -0.3265447015389533 2315 88-89 -0.38115689708383016 2315 90-91 -0.12758351882821728 2315 92-93 -1.2793767411287646 2315 94-95 -0.20149178205228324 2315 96-97 -0.042351066831912476 2315 98-99 -2.438258843892509 2315 100-101 -3.860894859105457 2316 1 -0.450387974621524 2316 2 -0.4441372005731239 2316 3 -0.8497498466254747 2316 4 -0.336404098019095 2316 5 -0.2342676170177853 2316 6 -0.661340823358401 2316 7 -0.8554438117349648 2316 8 -1.2316445746597608 2316 9 -5.092174398135636 2316 10-11 -1.4132435443245441 2316 12-13 -0.6356162153453013 2316 14-15 -0.4952961073969888 2316 16-17 -0.39988486040313376 2316 18-19 -0.44060106081914086 2316 20-21 -3.999054681597947 2316 22-23 -1.265475376643444 2316 24-25 -0.5209549802994573 2316 26-27 -0.8008438203549773 2316 28-29 -0.24620586154764368 2316 30-31 0.0407919495133342 2316 32-33 -1.2786261675594517 2316 34-35 -0.609550456433162 2316 36-37 -0.5162366931285902 2316 38-39 -5.707216165444311 2316 40-41 -5.369560161266051 2316 42-43 -3.8825041340683413 2316 44-45 -2.669237638942036 2316 46-47 -2.6563451478783513 2316 48-49 -3.0149699116068263 2316 50-51 -1.485938230513593 2316 52-53 -1.1971437766523074 2316 54-55 -0.3607394057739235 2316 56-57 -3.7556326993788787 2316 58-59 -1.1179104864995821 2316 60-61 -0.8780460115992952 2316 62-63 -0.5516980901804303 2316 64-65 -0.36828664702668945 2316 66-67 -0.4067535118746939 2316 68-69 2.2515978612727316 2316 70-71 3.2873051094285763 2316 72-73 1.9508205795580764 2316 74-75 0.5592595378407381 2316 76-77 -1.5227604668676307 2316 78-79 -0.13138805130294884 2316 80-81 -0.28895670500294557 2316 82-83 -0.2215114051464404 2316 84-85 -0.06706003015425566 2316 86-87 -0.021359402845003927 2316 88-89 -0.17414179343286307 2316 90-91 -0.10912476704044849 2316 92-93 -0.17074164910808065 2316 94-95 -0.044800440326284274 2316 96-97 0.33452235168537925 2316 98-99 0.5933664881206262 2316 100-101 0.6482887421958772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 32.0 4 115.0 5 282.0 6 647.0 7 1414.0 8 2944.0 9 4851.0 10 7672.0 11 11151.0 12 14049.0 13 17808.0 14 21851.0 15 27476.0 16 31927.0 17 35143.0 18 31343.0 19 26335.0 20 25688.0 21 26154.0 22 27437.0 23 30382.0 24 35734.0 25 44110.0 26 54712.0 27 66653.0 28 81117.0 29 96644.0 30 112845.0 31 129310.0 32 148924.0 33 170051.0 34 193588.0 35 212042.0 36 208633.0 37 162807.0 38 63320.0 39 3452.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.59550919741516 20.800174414698198 15.765735499546343 21.838580888340292 2 16.109410210291482 21.73943890946885 38.95654903199009 23.194601848249576 3 18.859893904288363 26.107465597817203 30.91418762367028 24.118452874224154 4 12.910202671092627 18.247404018628533 36.43923276026227 32.40316055001657 5 12.916359939623904 38.722161437939505 33.57177788624471 14.789700736191879 6 30.816989303584375 37.22478539285568 17.07569608443258 14.882529219127367 7 27.436548020653394 34.43809876368057 20.24398482235513 17.8813683933109 8 26.7780577143241 34.883403009503176 19.180168528795384 19.158370747377337 9 26.83236425596045 16.540851814078078 20.363825642177204 36.26295828778427 10-11 25.149067164442073 27.525473324365734 26.26908531889872 21.056374192293468 12-13 26.16938755280912 25.1886048339399 27.368641354619367 21.273366258631615 14-15 22.87869769530742 26.017390307597697 26.728895261190505 24.375016735904374 16-17 21.2585456258023 29.548927194380468 27.273914539631384 21.918612640185845 18-19 21.043522787863697 26.822524082108185 31.274020449532685 20.85993268049543 20-21 22.194651369384548 25.735381510501693 31.376802368817525 20.69316475129624 22-23 21.66435260600187 25.993286366240444 31.293655825149557 21.04870520260813 24-25 21.354452825854153 26.221814335475678 31.5931487867653 20.830584051904868 26-27 21.067357335257007 26.60618148701555 31.32741275755902 20.999048420168418 28-29 20.87077338657584 26.629326443126434 31.591473368958834 20.90842680133889 30-31 20.942050261785024 26.35983755000223 31.49589764428712 21.202214543925624 32-33 20.889128791055157 26.519258373458616 31.175552716504463 21.41606011898177 34-35 21.485364223710157 26.506052680103494 31.07719736305863 20.931385733127723 36-37 21.495955701020446 26.208599595358518 30.996379102895826 21.299065600725214 38-39 21.157156769297977 26.502027406072298 31.074424118396944 21.26639170623279 40-41 21.2155276677821 26.376419120559575 31.20571327153202 21.202339940126304 42-43 21.27074914370479 26.504787495386466 30.726269985119153 21.49819337578959 44-45 21.28540330618923 26.809659037481424 30.514899479058922 21.39003817727042 46-47 21.51720937623857 27.090327891897836 29.78724004455204 21.605222687311556 48-49 21.664724247827884 27.153699003278213 29.504582118244176 21.676994630649723 50-51 21.64356648365326 27.471168747888825 29.11189565203492 21.773369116422995 52-53 21.95878161598684 27.504383821846556 28.920015727937702 21.616818834228898 54-55 21.471077324539717 27.536327248867742 29.033386852064574 21.959208574527967 56-57 21.77128767864846 27.768058449424878 28.775641875095744 21.68501199683092 58-59 21.71784557776476 27.235493004205725 28.928824580161887 22.117836837867625 60-61 21.614619672847468 27.419333619938307 29.333995813687974 21.63205089352625 62-63 21.64978868859144 27.39024303160933 29.20814126764295 21.751827012156276 64-65 21.434972585430778 27.284549392618302 29.08457819195155 22.19589982999937 66-67 21.34717710211595 27.640396726507532 29.037282278495308 21.975143892881206 68-69 21.87406228021268 26.927099948031984 29.44582121298462 21.753016558770717 70-71 21.294025506834675 27.095547990178808 29.857366152171533 21.75306035081498 72-73 21.693833714046857 27.351726248391824 28.976279453234245 21.97816058432708 74-75 21.511715083307834 27.730812781936866 28.543397301302814 22.21407483345249 76-77 20.921420249261512 27.888479598921982 29.090113097409702 22.099987054406796 78-79 21.528784043091022 27.801278184042715 28.472132167349827 22.197805605516432 80-81 21.268607194895562 28.10467173280196 28.443301480307166 22.183419591995314 82-83 21.62835671580403 28.07871937958057 27.994416112376797 22.298507792238603 84-85 21.341163642803906 27.96721304544215 28.026828252534525 22.66479505921942 86-87 21.526592865974887 27.79132074549848 28.101569282576094 22.580517105950534 88-89 21.383348201932964 27.854806362915035 27.91909602672496 22.842749408427043 90-91 21.20954085442612 28.016376394933364 28.140568832842426 22.63351391779809 92-93 21.39208610194797 27.67296838476397 28.2478442448873 22.68710126840076 94-95 21.07812058120567 27.493137388345186 28.330309012054265 23.09843301839488 96-97 21.317526350715 27.31385313529802 28.29487328910687 23.073747224880112 98-99 21.042047540582306 27.45731510494853 27.947370918687852 23.55326643578131 100-101 20.908438249992013 27.25587260777802 28.399971621288923 23.435717520941047 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3272.0 1 2263.0 2 1286.5 3 1361.5 4 1304.5 5 1314.0 6 1981.0 7 3682.0 8 5550.5 9 6485.0 10 6000.5 11 4979.0 12 4676.5 13 4738.0 14 4783.5 15 4868.5 16 5004.0 17 5229.5 18 5527.5 19 5909.0 20 6493.5 21 7307.5 22 8330.0 23 9646.0 24 11117.0 25 13023.0 26 15831.5 27 18847.0 28 21134.5 29 23873.0 30 28095.5 31 32840.5 32 37892.0 33 42109.5 34 45563.5 35 48358.0 36 51611.5 37 55982.0 38 58536.5 39 58946.5 40 59528.5 41 63151.5 42 67160.0 43 69058.5 44 69935.0 45 70743.0 46 70988.5 47 71674.5 48 74628.5 49 75362.0 50 72993.0 51 70109.5 52 68255.0 53 67828.0 54 66108.5 55 61873.0 56 56283.5 57 50616.0 58 45655.5 59 40124.0 60 34604.5 61 29382.0 62 23760.0 63 18031.5 64 13074.0 65 9921.0 66 7744.5 67 6153.5 68 4600.5 69 3277.0 70 2523.5 71 2084.0 72 1810.5 73 1534.0 74 1254.5 75 1017.0 76 673.5 77 376.5 78 233.0 79 145.0 80 94.5 81 65.5 82 40.5 83 23.0 84 13.5 85 11.0 86 6.5 87 3.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01860327896885219 2 0.017522785493388555 3 6.107137035229255E-4 4 0.085124094675657 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011932406207294083 12-13 0.0 14-15 0.0 16-17 4.4629078334367634E-4 18-19 9.395595438814239E-5 20-21 0.0 22-23 1.6442292017924916E-4 24-25 0.003311947392182019 26-27 0.004298484913257514 28-29 0.0015032952702102782 30-31 9.395595438814239E-5 32-33 5.402467377318188E-4 34-35 0.0025602997570768797 36-37 0.08625156612831471 38-39 0.0962108972934578 40-41 0.07889951269744257 42-43 0.08277519581595344 44-45 0.08160074638610165 46-47 0.08014442909308545 48-49 0.07441311587540876 50-51 0.07429567093242359 52-53 0.05790035689169275 54-55 0.049467809985356964 56-57 0.027951896430472363 58-59 0.022807807927721566 60-61 0.013717569340668787 62-63 0.0021140089737332036 64-65 0.006365515909796646 66-67 0.16277869097745667 68-69 0.7431446212330122 70-71 0.908554078933337 72-73 0.36917643377960846 74-75 0.05571588095216844 76-77 0.20585749606441997 78-79 0.015267842588073137 80-81 0.0036877712097345885 82-83 0.0015267842588073137 84-85 2.583788745673915E-4 86-87 0.001926097064956919 88-89 4.697797719407119E-4 90-91 0.004956176593974511 92-93 0.005191066479944867 94-95 0.08143632346592242 96-97 0.03856891927633245 98-99 0.015408776519655352 100-101 0.014140371135415428 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2128657.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.00014244619793 #Duplication Level Percentage of deduplicated Percentage of total 1 71.34950442976707 32.10737862805148 2 14.03045452706857 12.627449046059763 3 5.629658962384306 7.600063656924257 4 2.8703516726778995 5.166649365647519 5 1.6578774273419745 3.7302360194362514 6 1.0109302382640672 2.72952028350311 7 0.6926445018679491 2.1818370874043462 8 0.4838477306961078 1.7418573442875573 9 0.32782401666230754 1.327691470237974 >10 1.7025306676522527 13.833861532495927 >50 0.1410722503544888 4.398539036962678 >100 0.09616245468771108 7.923906493330716 >500 0.005009442791706733 1.4565374066623684 >1k 0.0018119261161492434 1.3232485021209193 >5k 1.065838891852496E-4 0.46893329244395326 >10k+ 2.131677783704992E-4 1.3822908344312912 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15175 0.7128908039200303 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13645 0.6410144988131015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9777 0.4593036830264341 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4455 0.20928688839958717 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3493 0.16409407433889067 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2609 0.12256554249933174 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.697797719407119E-5 0.0 4 0.0 0.0 0.0 4.697797719407119E-5 0.0 5 0.0 0.0 0.0 4.697797719407119E-5 0.0 6 0.0 0.0 0.0 4.697797719407119E-5 0.0 7 0.0 0.0 0.0 9.395595438814239E-5 0.0 8 0.0 0.0 0.0 9.395595438814239E-5 0.0 9 0.0 0.0 0.0 1.4093393158221357E-4 0.0 10-11 0.0 0.0 0.0 1.4093393158221357E-4 0.0 12-13 0.0 0.0 0.0 1.4093393158221357E-4 0.0 14-15 0.0 0.0 0.0 1.8791190877628477E-4 0.0 16-17 0.0 0.0 0.0 2.5837887456739157E-4 0.0 18-19 0.0 0.0 0.0 2.8186786316442713E-4 0.0 20-21 0.0 0.0 0.0 4.228017947466407E-4 0.0 22-23 0.0 0.0 0.0 6.107137035229255E-4 0.0 24-25 0.0 0.0 0.0 0.0010804934754636375 0.0 26-27 0.0 0.0 0.0 0.0014798062816132425 0.0 28-29 0.0 0.0 0.0 0.003311947392182019 0.0 30-31 0.0 0.0 0.0 0.0072346084878869635 0.0 32-33 0.0 0.0 0.0 0.015902045280193098 0.0 34-35 0.0 0.0 0.0 0.028938433951547854 0.0 36-37 0.0 0.0 0.0 0.05068923739240282 0.0 38-39 0.0 0.0 0.0 0.08322148659929712 0.0 40-41 0.0 0.0 0.0 0.12068642341156888 0.0 42-43 0.0 0.0 0.0 0.15810438224664658 0.0 44-45 0.0 0.0 0.0 0.2025220596836409 0.0 46-47 0.0 0.0 0.0 0.25837887456739156 0.0 48-49 0.0 0.0 0.0 0.3208126062583122 0.0 50-51 0.0 0.0 0.0 0.38895416217831247 0.0 52-53 0.0 4.697797719407119E-5 0.0 0.4583641234825526 0.0 54-55 0.0 4.697797719407119E-5 0.0 0.5328007283465583 0.0 56-57 0.0 4.697797719407119E-5 0.0 0.6207200126652626 0.0 58-59 0.0 4.697797719407119E-5 0.0 0.7122566012279103 0.0 60-61 0.0 4.697797719407119E-5 0.0 0.8051320621405891 0.0 62-63 0.0 4.697797719407119E-5 0.0 0.8982893909164322 2.3488988597035597E-5 64-65 0.0 4.697797719407119E-5 0.0 0.9944063322555019 4.697797719407119E-5 66-67 0.0 4.697797719407119E-5 0.0 1.0954559611999493 4.697797719407119E-5 68-69 0.0 4.697797719407119E-5 0.0 1.2164242524746824 4.697797719407119E-5 70-71 0.0 4.697797719407119E-5 0.0 1.3730488284397158 4.697797719407119E-5 72-73 0.0 4.697797719407119E-5 0.0 1.5485350622481686 4.697797719407119E-5 74-75 0.0 4.697797719407119E-5 0.0 1.7261587940189518 4.697797719407119E-5 76-77 0.0 4.697797719407119E-5 0.0 1.936901999711555 4.697797719407119E-5 78-79 0.0 4.697797719407119E-5 0.0 2.1724495773626282 4.697797719407119E-5 80-81 0.0 4.697797719407119E-5 0.0 2.439660311642505 4.697797719407119E-5 82-83 0.0 4.697797719407119E-5 0.0 2.71821622741475 4.697797719407119E-5 84-85 0.0 4.697797719407119E-5 0.0 2.99538629285977 4.697797719407119E-5 86-87 0.0 4.697797719407119E-5 0.0 3.2844887645120844 4.697797719407119E-5 88-89 0.0 4.697797719407119E-5 0.0 3.598466075088659 4.697797719407119E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7460 0.0 55.283134 1 GTATCAA 13155 0.0 40.297123 1 ATCAACG 17520 0.0 30.025806 3 TCAACGC 17775 0.0 29.576015 4 TATCAAC 17835 0.0 29.557026 2 GTGGTAT 3290 0.0 28.994247 1 CAACGCA 18230 0.0 28.70672 5 TGGTATC 3395 0.0 28.510847 2 AACGCAG 18840 0.0 27.777258 6 GTACATG 13520 0.0 25.027906 1 TACATGG 13540 0.0 24.565132 2 ACGCAGA 21640 0.0 24.09554 7 CGCAGAG 21780 0.0 23.940655 8 ACATGGG 13935 0.0 22.772797 3 GAGTACT 13180 0.0 22.598991 12-13 GCAGAGT 22400 0.0 22.431538 9 AGTACTT 13660 0.0 21.180399 12-13 GTACTTT 14215 0.0 20.853533 14-15 CATGGGG 4765 0.0 20.810999 4 GGACCGT 230 9.227551E-8 20.609781 6 >>END_MODULE