FastQCFastQC Report
Fri 27 May 2016
SRR939543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376365
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107692.861318135320766No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74521.9799928261129487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65071.7289067793232633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54761.4549705737781142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42171.120454877578946No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35840.9522670811579185No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14110.37490202330184796No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12000.31883942449483876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.30050615758638555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.1655308012169038No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.1493231304717495No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.10867110384865754No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.10654550768535863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC700.068.524233
TATAACG750.065.293542
GTACCGG300.00812739150.4587551
GTCTTAG501.9748568E-550.4587551
TACCTGG4400.050.083112
CGGATTG300.00889097549.3254059
TATCACG706.215487E-848.970152
ATGGGCG706.7935616E-848.4202275
ATCACGC751.2588134E-744.769173
GGTATCA35700.044.239751
ACAACGC554.6786576E-543.606333
ACCTGGG4750.043.4227263
GTACCTG5050.040.9665151
GGGATCA2100.040.847561
GTATAAC1405.456968E-1239.6461681
TAACGCA1357.2759576E-1239.0629964
TCACGCA905.287129E-737.2874034
GTATCAA59100.036.883561
GTATCAC1051.1831471E-633.6391681
TATATGG2350.033.341382
Produced by FastQC (version 0.11.5)