FastQCFastQC Report
Fri 27 May 2016
SRR939543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376365
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136393.6238757589042554No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125463.33346618309354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86252.2916583635566536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80572.14074103596243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65011.7273125822007893No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49901.3258406068577044No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.5731138655294727No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.5252879518552469No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17560.4665683578441141No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.2800472945146334No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.23726967172824254No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.1732360873088624No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.16499940217607909No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.10415421200164734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG505.653419E-966.35811
TATAACG659.094947E-1265.620182
CGGTATC352.8169164E-454.162682
ATCACGC352.822469E-454.1410983
TACCTGG4950.053.615582
ATAACGC851.3278623E-1050.160133
ACCTGGG5250.049.6293333
GGTATCA39400.048.6016541
ACACGGG502.8682074E-547.373453
TAACGCA953.929017E-1044.8801164
GTACCTG6050.044.6565741
TCACGCA459.743895E-442.1097374
GCGGTAT500.001631464337.9189151
TATCACG500.001632530237.9138762
TTCAACG500.001632530237.9138762
ATGGGCG500.001635731237.898765
GTATAAC1152.5447662E-937.0945931
GTATCAA61450.033.0132141
GCGTGGG300.00369183932.1069764-65
GTATATG3200.031.1053581
Produced by FastQC (version 0.11.5)