##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939543_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 376365 Sequences flagged as poor quality 0 Sequence length 101 %GC 30 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97768655427577 31.0 31.0 31.0 30.0 34.0 2 31.044714572290196 31.0 31.0 33.0 28.0 34.0 3 30.985798360633957 31.0 31.0 34.0 27.0 34.0 4 34.95913541376058 35.0 35.0 37.0 32.0 37.0 5 34.68417626506184 35.0 35.0 37.0 32.0 37.0 6 34.7384666480677 35.0 35.0 37.0 32.0 37.0 7 34.666143238611454 35.0 35.0 37.0 31.0 37.0 8 34.72566258817903 35.0 35.0 37.0 31.0 37.0 9 36.016343177500566 38.0 35.0 39.0 30.0 39.0 10-11 35.85820147994633 37.0 35.0 39.0 30.0 39.0 12-13 35.808404075830644 37.0 35.0 39.0 30.0 39.0 14-15 36.455763686846545 38.0 35.0 40.0 30.0 41.0 16-17 36.529920422993634 38.0 35.5 40.0 30.5 41.0 18-19 36.6359544591022 38.5 35.0 40.0 31.0 41.0 20-21 36.534032123072016 38.0 35.0 40.0 30.0 41.0 22-23 36.15271478484981 38.0 34.5 40.0 30.0 41.0 24-25 35.82842054920091 38.0 34.0 40.0 29.5 41.0 26-27 35.317465757974304 37.0 34.0 40.0 27.5 41.0 28-29 34.92111248389197 37.0 33.0 40.0 27.0 41.0 30-31 34.404705538506505 36.0 33.0 39.0 26.0 40.5 32-33 33.666286716352474 35.0 33.0 39.0 24.5 40.0 34-35 32.959294833472825 35.0 32.5 39.0 21.5 40.0 36-37 32.482851752952584 35.0 31.5 39.0 18.5 41.0 38-39 32.356942064219574 35.0 31.0 39.0 18.0 41.0 40-41 32.036351679885215 35.0 31.0 39.0 16.5 40.5 42-43 31.56573538984762 35.0 31.0 39.0 13.5 40.0 44-45 31.144974957820203 35.0 30.5 39.0 8.0 40.0 46-47 30.476059144713243 35.0 29.5 38.0 2.0 40.0 48-49 29.93999442031007 35.0 27.0 38.0 2.0 40.0 50-51 29.671130418609593 35.0 27.0 38.0 2.0 40.0 52-53 29.363458344957685 35.0 27.0 38.0 2.0 40.0 54-55 29.19186295218737 35.0 26.5 38.0 2.0 40.0 56-57 28.869198783096195 34.5 25.5 38.0 2.0 40.0 58-59 28.53760711011917 34.0 25.0 38.0 2.0 40.0 60-61 28.336993344227015 34.0 26.0 37.0 2.0 40.0 62-63 28.00446109494772 33.5 25.0 37.0 2.0 40.0 64-65 27.682559483480134 33.0 23.0 36.5 2.0 39.5 66-67 26.377144527254128 32.5 18.0 35.5 2.0 39.0 68-69 23.372534640574973 29.0 10.0 35.0 2.0 38.0 70-71 22.948199221500403 28.5 8.5 34.5 2.0 37.0 72-73 24.45673880408646 31.0 11.5 35.0 2.0 37.0 74-75 24.922701367024032 31.0 15.0 35.0 2.0 37.0 76-77 24.78059994951709 30.5 19.0 34.5 2.0 36.0 78-79 25.498498797709672 32.5 20.0 35.0 2.0 36.0 80-81 25.349748249704408 33.0 18.0 35.0 2.0 36.0 82-83 24.954408619292444 32.0 14.5 35.0 2.0 36.0 84-85 24.623525035537313 32.0 9.5 35.0 2.0 35.5 86-87 24.192046284856456 31.5 4.5 35.0 2.0 35.0 88-89 23.706068577046217 31.0 2.0 34.5 2.0 35.0 90-91 23.27196338660609 31.0 2.0 34.0 2.0 35.0 92-93 22.799452658987953 31.0 2.0 34.0 2.0 35.0 94-95 21.678989810423392 29.0 2.0 34.0 2.0 35.0 96-97 20.936527041568688 29.0 2.0 34.0 2.0 35.0 98-99 20.661181300067753 29.0 2.0 34.0 2.0 35.0 100-101 19.850942569048662 28.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3726235215211453 1101 2 0.04062444601732906 1101 3 0.19032273689235524 1101 4 0.2172329817658536 1101 5 0.2574572690266166 1101 6 0.635891418742716 1101 7 0.6547353092610564 1101 8 0.7356349397926039 1101 9 0.6134456234263723 1101 10-11 0.6965442543279963 1101 12-13 0.7484276911756282 1101 14-15 0.722521107571616 1101 16-17 0.6116151154324214 1101 18-19 0.920911272270061 1101 20-21 0.9713823804318977 1101 22-23 0.8962890048293204 1101 24-25 0.6412532805923874 1101 26-27 0.7066083935657375 1101 28-29 0.5842804580143266 1101 30-31 0.8813129678758429 1101 32-33 0.930484743985609 1101 34-35 0.9782408569640992 1101 36-37 0.778266598814966 1101 38-39 0.695598869011107 1101 40-41 1.3068246742027405 1101 42-43 1.5647734504241164 1101 44-45 1.2683690821955835 1101 46-47 0.9635596388763226 1101 48-49 1.236010419508144 1101 50-51 1.3410841143112506 1101 52-53 1.4087252746767547 1101 54-55 1.0012557900575167 1101 56-57 1.096456537255861 1101 58-59 0.6970710494679793 1101 60-61 0.8619582859558079 1101 62-63 1.1257979191262741 1101 64-65 1.1117750334578496 1101 66-67 -1.56586900496902 1101 68-69 -6.718437717068358 1101 70-71 -8.735051782640515 1101 72-73 -9.328106688360748 1101 74-75 -7.409133213694631 1101 76-77 -2.5112003391366926 1101 78-79 -0.3592699927120613 1101 80-81 0.24750441891457697 1101 82-83 0.34767426799816903 1101 84-85 0.07938205534083309 1101 86-87 -0.11058052602882285 1101 88-89 0.12381358821898303 1101 90-91 0.3326618430234909 1101 92-93 0.04921302475268163 1101 94-95 -3.4106630928639987 1101 96-97 -0.7546094457664907 1101 98-99 0.3915666827863653 1101 100-101 0.5823448826141799 1102 1 0.3610640343128395 1102 2 0.48101682638867516 1102 3 0.6589475592856502 1102 4 0.7629852715878869 1102 5 0.7539613268882661 1102 6 0.8183547133571167 1102 7 0.6495174851739733 1102 8 0.566922618503078 1102 9 0.7729264097926034 1102 10-11 1.004484177104196 1102 12-13 0.7399708024437146 1102 14-15 0.851887021533706 1102 16-17 1.0937862118979496 1102 18-19 1.0977914951112808 1102 20-21 0.9540953278603155 1102 22-23 1.1185040915656117 1102 24-25 1.270450618699357 1102 26-27 0.9104326572504817 1102 28-29 0.7062210069294252 1102 30-31 0.9156261818146518 1102 32-33 1.248266285732214 1102 34-35 1.12967816681455 1102 36-37 1.330111701686377 1102 38-39 1.2113005058023845 1102 40-41 1.0433245578051178 1102 42-43 1.312579957452126 1102 44-45 1.182848945408324 1102 46-47 1.0062173576436315 1102 48-49 1.072182784306296 1102 50-51 1.2408095764900509 1102 52-53 1.4350231080756437 1102 54-55 1.1584327064037545 1102 56-57 0.8131086207731535 1102 58-59 0.8082829493178636 1102 60-61 1.0178829245211496 1102 62-63 0.7009506408235922 1102 64-65 0.5707348401575381 1102 66-67 -1.692927035200288 1102 68-69 -6.398500812602705 1102 70-71 -9.202701273300608 1102 72-73 -6.976417317469785 1102 74-75 -5.3989945801223485 1102 76-77 -2.6361832808656374 1102 78-79 -0.17985310462234239 1102 80-81 0.21935626482018833 1102 82-83 0.5399952364263179 1102 84-85 0.26676615944043647 1102 86-87 0.4887828994325538 1102 88-89 0.9819650335562535 1102 90-91 1.036140927576323 1102 92-93 0.09077500046170428 1102 94-95 -2.75892074099 1102 96-97 0.022589465715132206 1102 98-99 1.061294472917453 1102 100-101 1.1633295261565202 1103 1 0.32888552920606173 1103 2 0.18965482546017043 1103 3 0.2022028401584457 1103 4 0.049530652274981435 1103 5 0.27266200005447416 1103 6 0.23000164929026568 1103 7 0.28248957198920266 1103 8 0.3497829945476454 1103 9 0.5017666488826649 1103 10-11 0.4793927773827491 1103 12-13 0.2649570199460456 1103 14-15 0.4416763669376067 1103 16-17 0.3555775013594129 1103 18-19 0.22559877227006098 1103 20-21 0.39691972433199396 1103 22-23 0.4999528496900325 1103 24-25 0.5298444789593404 1103 26-27 0.9710176154677512 1103 28-29 0.8039497963908886 1103 30-31 1.3505582848787228 1103 32-33 1.3970846179049161 1103 34-35 1.1715007933233679 1103 36-37 0.8735700930512778 1103 38-39 1.2070061696835381 1103 40-41 0.895133691974106 1103 42-43 1.2009743690120125 1103 44-45 1.176037489976565 1103 46-47 1.806142641998317 1103 48-49 2.092646166386146 1103 50-51 1.5599541911604362 1103 52-53 1.2488542071935633 1103 54-55 1.3193506207491588 1103 56-57 1.7120792858149372 1103 58-59 2.0749416366437714 1103 60-61 1.636078723035542 1103 62-63 1.3597939265710366 1103 64-65 1.6027392505567946 1103 66-67 -1.2042613260545174 1103 68-69 -5.154804491804189 1103 70-71 -9.290669878221687 1103 72-73 -6.5853788425394235 1103 74-75 -5.1852461759905 1103 76-77 -2.196887929194329 1103 78-79 0.11245190930523208 1103 80-81 0.7856138089241789 1103 82-83 1.1182036568070046 1103 84-85 0.729560669653992 1103 86-87 0.8711565356906767 1103 88-89 0.6262714112257051 1103 90-91 0.8862236225623938 1103 92-93 0.7222962980475884 1103 94-95 1.2474518506998784 1103 96-97 1.076018406058676 1103 98-99 0.49790223754140683 1103 100-101 0.2759688019225379 1104 1 0.5176158441666914 1104 2 0.3620219514444223 1104 3 0.39243003832117296 1104 4 0.2332986968944013 1104 5 0.376951698217205 1104 6 0.47909941831913727 1104 7 0.4710763502044202 1104 8 0.3766448711880628 1104 9 0.516468912662198 1104 10-11 0.7286340831570257 1104 12-13 0.6735753762452532 1104 14-15 1.1114188210058487 1104 16-17 0.8597298143515459 1104 18-19 1.0350845006165201 1104 20-21 1.0378728083215023 1104 22-23 0.8040662027084053 1104 24-25 0.6976799448386117 1104 26-27 1.1317787703233932 1104 28-29 1.103398205183538 1104 30-31 0.8668394528577252 1104 32-33 0.15726547420413084 1104 34-35 0.8243571745569724 1104 36-37 0.8289711757284408 1104 38-39 0.21258359750505917 1104 40-41 0.5281102339688637 1104 42-43 1.0308115573322247 1104 44-45 0.8499835201602934 1104 46-47 0.5140146072214158 1104 48-49 0.4362116191420533 1104 50-51 0.33037741950688826 1104 52-53 0.7912693154612818 1104 54-55 0.67065680513236 1104 56-57 0.9172035476259595 1104 58-59 0.6645803341503331 1104 60-61 0.790483250594594 1104 62-63 0.6746417776209093 1104 64-65 0.1743435812654539 1104 66-67 -1.4798826351096324 1104 68-69 -6.737215088917051 1104 70-71 -7.86040823124006 1104 72-73 -6.857257943589293 1104 74-75 -6.556606494231968 1104 76-77 -3.6967956555722843 1104 78-79 -1.0143525526370247 1104 80-81 0.11701964881919125 1104 82-83 0.3033763110064811 1104 84-85 0.33686463947026724 1104 86-87 0.2859264667930397 1104 88-89 -0.3780244385118259 1104 90-91 -0.4801846779625407 1104 92-93 -0.6611313594327228 1104 94-95 0.12699376342953883 1104 96-97 0.003153002515372094 1104 98-99 0.09688107612408459 1104 100-101 0.05861315887791818 1105 1 0.8016011234866696 1105 2 0.6305758486952016 1105 3 0.603670389309471 1105 4 0.2856091822124256 1105 5 0.49525192409379315 1105 6 0.4944956171186874 1105 7 0.6295558700231609 1105 8 0.4888161714913437 1105 9 0.6596014902588934 1105 10-11 0.8078738430663961 1105 12-13 0.7587188888021643 1105 14-15 0.9290674527284963 1105 16-17 0.7884661016721992 1105 18-19 1.016085535004649 1105 20-21 0.8645107654401301 1105 22-23 1.0049028608607173 1105 24-25 1.1490566661800727 1105 26-27 1.0936717709637307 1105 28-29 1.362026902065594 1105 30-31 1.3990251079350244 1105 32-33 1.4680568866716683 1105 34-35 1.2502506536897684 1105 36-37 1.0872304482791577 1105 38-39 0.9696290472527949 1105 40-41 0.942769695101898 1105 42-43 1.2423931860361357 1105 44-45 1.2761338371615523 1105 46-47 0.3693163741252192 1105 48-49 0.7819963116050914 1105 50-51 0.8788494101059214 1105 52-53 1.1069969406609452 1105 54-55 0.7441265088188622 1105 56-57 0.7698771242867863 1105 58-59 0.8002997968314389 1105 60-61 0.9319762878255311 1105 62-63 1.1131695688856027 1105 64-65 0.6962099833539348 1105 66-67 1.364881742533509 1105 68-69 -4.3043869873359135 1105 70-71 -4.305212717810605 1105 72-73 -0.9289403371774938 1105 74-75 1.026218440854894 1105 76-77 1.2230287095456163 1105 78-79 1.3341342149352577 1105 80-81 1.292246404992099 1105 82-83 1.4953784501492784 1105 84-85 1.4594185226477343 1105 86-87 1.3942135341267807 1105 88-89 1.6360981259263347 1105 90-91 1.359309245886788 1105 92-93 1.643703525482799 1105 94-95 2.4553697740689593 1105 96-97 1.883097131483165 1105 98-99 1.636705019042747 1105 100-101 1.5459084802067196 1106 1 0.30216451471330785 1106 2 0.1130394872959215 1106 3 0.14721340779129477 1106 4 0.27379091797546096 1106 5 0.3128944879771751 1106 6 0.43516469276852376 1106 7 0.6435629415544142 1106 8 0.5180348061418485 1106 9 0.4461733517812192 1106 10-11 0.4006261711025516 1106 12-13 0.24844509361754064 1106 14-15 0.5148194828796377 1106 16-17 0.5492482441130377 1106 18-19 0.2457904993232063 1106 20-21 0.11302781611943402 1106 22-23 0.5840787554871341 1106 24-25 0.3693296842733531 1106 26-27 0.4818871806851419 1106 28-29 0.6411516381783287 1106 30-31 0.4374393235260712 1106 32-33 0.6717487181464605 1106 34-35 1.0485954491204765 1106 36-37 1.0122702742106995 1106 38-39 1.3271752518091375 1106 40-41 1.1423785591238662 1106 42-43 1.3213132880941423 1106 44-45 1.2865824778992732 1106 46-47 1.1419103050417974 1106 48-49 1.1470317340189986 1106 50-51 1.006059263624202 1106 52-53 0.9188987422177171 1106 54-55 1.3121608562564049 1106 56-57 0.8883511759115557 1106 58-59 1.1654647344698574 1106 60-61 1.168008070861628 1106 62-63 1.1494791620782863 1106 64-65 1.2179264486244286 1106 66-67 1.9832198031725383 1106 68-69 -4.496629672963611 1106 70-71 -4.191145421699947 1106 72-73 -1.0060083715249313 1106 74-75 0.8627799411592569 1106 76-77 0.5595092628346556 1106 78-79 0.8700002184839768 1106 80-81 0.5666373596488192 1106 82-83 0.7305036266137712 1106 84-85 0.6092028797989215 1106 86-87 0.6905443678162797 1106 88-89 0.8071100283754653 1106 90-91 0.5650053254609446 1106 92-93 0.21229478838575488 1106 94-95 0.9526337649510879 1106 96-97 1.1101178927736548 1106 98-99 0.9420824911645944 1106 100-101 1.1748214589273687 1107 1 -0.03202133353903491 1107 2 -0.022048605912377894 1107 3 -0.05223956180233813 1107 4 -0.05861885752894125 1107 5 0.09404680397604182 1107 6 0.2342908649765434 1107 7 0.18000795434214467 1107 8 0.15891289650843277 1107 9 0.2889909135885489 1107 10-11 -0.0982726637937219 1107 12-13 0.03341596602447083 1107 14-15 0.41502195517289664 1107 16-17 0.3135278690064709 1107 18-19 0.3326759781524089 1107 20-21 0.07676041060650363 1107 22-23 0.4890766241998321 1107 24-25 0.46964227307698536 1107 26-27 0.2749391840952029 1107 28-29 0.2262077620771592 1107 30-31 0.2301538731140127 1107 32-33 0.4631844953558968 1107 34-35 0.5013874354802326 1107 36-37 0.021701220502258423 1107 38-39 0.1910747971345117 1107 40-41 0.13701481942508664 1107 42-43 0.04449458960024444 1107 44-45 0.19273479389813275 1107 46-47 -0.3092832158448182 1107 48-49 -0.06590162773150254 1107 50-51 0.30934144606239755 1107 52-53 0.3449785944484667 1107 54-55 0.5110632432981781 1107 56-57 0.31203332993258215 1107 58-59 0.5940133278202424 1107 60-61 0.9727821544080903 1107 62-63 0.45009723539456914 1107 64-65 0.196489250556791 1107 66-67 0.7126351200239149 1107 68-69 -5.577139050627721 1107 70-71 -4.969438260574627 1107 72-73 -1.5138408523433462 1107 74-75 0.2557005151859677 1107 76-77 0.17129528158998397 1107 78-79 -0.14245005147908074 1107 80-81 -0.3678788381346436 1107 82-83 0.3047079460226918 1107 84-85 0.2575937578892855 1107 86-87 0.5458654817691091 1107 88-89 0.3140011906374731 1107 90-91 0.18600916177808102 1107 92-93 0.6970205627534725 1107 94-95 1.3654818752096816 1107 96-97 1.1154638717449537 1107 98-99 0.8160701296982715 1107 100-101 0.5688609587852831 1108 1 -0.14996548089494866 1108 2 -0.14036411393376724 1108 3 0.3261769563200616 1108 4 0.3018980765174035 1108 5 0.02787033338780276 1108 6 0.36983750787612735 1108 7 0.1324737891609189 1108 8 -0.1450086721190189 1108 9 0.1876094519129694 1108 10-11 0.049231792534264684 1108 12-13 0.16335506287533264 1108 14-15 0.011909952796195 1108 16-17 0.08075900388467261 1108 18-19 0.4322749086337012 1108 20-21 0.3506760374633089 1108 22-23 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3.48011771283716 2311 70-71 3.9344553854913933 2311 72-73 1.8555032777137406 2311 74-75 0.8644340718997938 2311 76-77 0.5635452195398507 2311 78-79 0.4765341877862461 2311 80-81 0.3628949270676394 2311 82-83 0.2924158139800035 2311 84-85 0.3808594565738268 2311 86-87 0.4291305599522808 2311 88-89 0.6296502350653697 2311 90-91 0.7673351731953062 2311 92-93 0.8028408655581387 2311 94-95 1.2220002420500933 2311 96-97 1.5240174830628987 2311 98-99 1.3959441679211544 2311 100-101 1.0932156373655744 2312 1 -0.10212862173733228 2312 2 -0.14751105504297257 2312 3 0.018191440787376223 2312 4 -0.018570762819358322 2312 5 -0.06961394334175708 2312 6 0.11030825306384884 2312 7 -0.017087865117716206 2312 8 0.1647397555539314 2312 9 -1.1767416058343159 2312 10-11 -0.961988905007189 2312 12-13 -2.0446685696765954 2312 14-15 -1.893917186521513 2312 16-17 -0.07585429895505058 2312 18-19 -1.931289985591576 2312 20-21 -2.359467854284354 2312 22-23 -1.263952417605573 2312 24-25 -1.5116747820469456 2312 26-27 -1.9645169757272143 2312 28-29 -0.9524132146154045 2312 30-31 -0.18570350757411092 2312 32-33 -0.20248790567998753 2312 34-35 -0.20824075541876397 2312 36-37 0.22002253015819662 2312 38-39 0.29741497471498235 2312 40-41 0.5479933617854336 2312 42-43 0.6371231504585531 2312 44-45 0.8160237321149282 2312 46-47 1.0023150083505215 2312 48-49 1.0714688865119335 2312 50-51 1.0290972729214403 2312 52-53 0.8039593565646364 2312 54-55 0.7113268393026182 2312 56-57 1.1329961490224854 2312 58-59 0.7490031539393698 2312 60-61 0.8144395720921445 2312 62-63 0.32851935896097473 2312 64-65 0.5498697537014472 2312 66-67 1.7587644208008335 2312 68-69 4.004737567944236 2312 70-71 4.287799068319192 2312 72-73 2.7404368021146652 2312 74-75 2.312419901053527 2312 76-77 1.1404499245792543 2312 78-79 0.902153167166869 2312 80-81 1.2599652240185222 2312 82-83 1.3151130514610934 2312 84-85 0.9274282011005361 2312 86-87 1.0035513863196073 2312 88-89 1.3212153970747629 2312 90-91 1.4955494873422701 2312 92-93 1.7037723200601675 2312 94-95 2.278333336548936 2312 96-97 2.570490693794529 2312 98-99 1.8395394230759976 2312 100-101 1.6348524497212793 2313 1 -0.2864161949318671 2313 2 -0.06878626649385211 2313 3 -0.3158467288070739 2313 4 -0.3292408994491609 2313 5 -0.408911275807597 2313 6 -0.15541501737639862 2313 7 -2.805962295826891 2313 8 -0.8892615456822384 2313 9 -0.8194438396230765 2313 10-11 -0.06936799847931496 2313 12-13 -1.7962408967206258 2313 14-15 -0.7980865640968702 2313 16-17 -0.4792375130083997 2313 18-19 -0.02736458979890699 2313 20-21 0.08495851743544591 2313 22-23 -1.0908337882410066 2313 24-25 -2.1559493428797367 2313 26-27 -1.856568591428804 2313 28-29 -0.6915153616550924 2313 30-31 -0.2372649909833484 2313 32-33 -2.2550795343939356 2313 34-35 -3.2498803129984637 2313 36-37 -1.8416777517763094 2313 38-39 -1.259947853304972 2313 40-41 -1.2974848425086485 2313 42-43 -1.4656473982293683 2313 44-45 -1.4452807571498703 2313 46-47 -1.8924475160476568 2313 48-49 -1.7786720807402894 2313 50-51 -1.0754624755062352 2313 52-53 -1.13930132441563 2313 54-55 -0.8683738763772801 2313 56-57 -0.493874377978166 2313 58-59 -1.3206671995631254 2313 60-61 -0.2074916792633097 2313 62-63 -0.29542338664735013 2313 64-65 -0.4507808643857345 2313 66-67 0.6085529699224956 2313 68-69 3.2078930656670757 2313 70-71 3.1767331390196922 2313 72-73 1.6832095195295445 2313 74-75 0.899928033779613 2313 76-77 0.4235772288516486 2313 78-79 -0.07648487230396128 2313 80-81 -0.13482440946662066 2313 82-83 -0.3800452656067854 2313 84-85 -0.03310096827704001 2313 86-87 0.018651147759641162 2313 88-89 -0.20602923532601736 2313 90-91 -0.23222106134565124 2313 92-93 0.3917467290820724 2313 94-95 0.973789853573443 2313 96-97 1.02101661008167 2313 98-99 0.972129967426465 2313 100-101 1.062606732957022 2314 1 -0.6086816750950135 2314 2 -0.34720673367961297 2314 3 -0.12116745554047981 2314 4 -0.46858090686480836 2314 5 -2.0742802042466053 2314 6 -3.064554802322636 2314 7 -1.2906119065054256 2314 8 -0.5670516828717069 2314 9 -0.18047461455819303 2314 10-11 0.042951245124683624 2314 12-13 -0.15753121930126923 2314 14-15 -2.831206697578523 2314 16-17 -2.9454221626190744 2314 18-19 -0.6710242116009084 2314 20-21 -0.19605742620563404 2314 22-23 -0.36760427396588824 2314 24-25 -2.09977581673958 2314 26-27 -1.5434985135645078 2314 28-29 -0.5152454321037396 2314 30-31 -0.4096231667341854 2314 32-33 -0.5700776804821786 2314 34-35 -0.75631338678415 2314 36-37 -0.2413996650132404 2314 38-39 -1.8814346905315205 2314 40-41 -3.997725851036641 2314 42-43 -5.542649622923474 2314 44-45 -2.4156459777653723 2314 46-47 -3.0704786617651187 2314 48-49 -1.0364028927536388 2314 50-51 -1.1655162389470917 2314 52-53 -2.8013054030214875 2314 54-55 -1.2718036131218078 2314 56-57 -3.0499922867657077 2314 58-59 -4.506484774646552 2314 60-61 -2.432436061910696 2314 62-63 -1.5688939094504661 2314 64-65 -0.46735483546471457 2314 66-67 -0.49444109755989274 2314 68-69 1.6462875780882804 2314 70-71 1.5935203099503568 2314 72-73 -0.506195374797489 2314 74-75 -0.16872231846361885 2314 76-77 -0.20860667919432885 2314 78-79 0.16608094156329756 2314 80-81 0.27771730354783486 2314 82-83 0.17063141218335076 2314 84-85 -0.1260004862599864 2314 86-87 0.012041952357343177 2314 88-89 0.3546400268171048 2314 90-91 0.17921757417529705 2314 92-93 0.04610342170350634 2314 94-95 0.7612203318826758 2314 96-97 0.8003547635855597 2314 98-99 0.20017038270269794 2314 100-101 0.5392752535354397 2315 1 -0.336519603435228 2315 2 -0.28689866358250526 2315 3 -0.40647831612760044 2315 4 -0.3328403108161382 2315 5 -1.2858551568082746 2315 6 -0.4031082123014329 2315 7 -0.12477866618842626 2315 8 -0.40308248988142026 2315 9 -0.02170689494663236 2315 10-11 -1.6090858125826486 2315 12-13 -1.7010851873780624 2315 14-15 -0.8659036503634283 2315 16-17 -1.9168642878562707 2315 18-19 -2.7486389007403176 2315 20-21 -1.9245371672481681 2315 22-23 -2.034207689525651 2315 24-25 -1.6451212794028294 2315 26-27 -0.7530308274388275 2315 28-29 -0.9826301848663235 2315 30-31 -3.2073461268859838 2315 32-33 -3.455572713409964 2315 34-35 -2.7539470512556363 2315 36-37 -5.673440071308583 2315 38-39 -3.313899251308392 2315 40-41 -2.795655307449188 2315 42-43 -2.7621663100537326 2315 44-45 -2.4831590930684158 2315 46-47 -1.7902520739763084 2315 48-49 -4.184918352068291 2315 50-51 -3.6707623601659805 2315 52-53 -2.558856468988669 2315 54-55 -3.4326362953408065 2315 56-57 -5.87330404030963 2315 58-59 -6.2007531081659195 2315 60-61 -4.983100198533091 2315 62-63 -6.379631715009122 2315 64-65 -5.204390218878039 2315 66-67 -3.873942157946093 2315 68-69 -1.8535952213312932 2315 70-71 0.09551271981753118 2315 72-73 -1.159962536311049 2315 74-75 -2.6879990234530133 2315 76-77 -4.374696644592252 2315 78-79 -4.007141516300305 2315 80-81 -3.7873569234864313 2315 82-83 -6.722252838291034 2315 84-85 -4.805194592745085 2315 86-87 -4.338650089165146 2315 88-89 -3.121696617897708 2315 90-91 -3.2344781507940077 2315 92-93 -3.420338145435686 2315 94-95 -1.6850515734639018 2315 96-97 -1.4664997960751158 2315 98-99 -2.6952476327008092 2315 100-101 -1.9439706444442422 2316 1 -0.30166495741194055 2316 2 -0.35912102612133623 2316 3 -1.2960673849023827 2316 4 -0.49582366645981324 2316 5 -0.3285773551766695 2316 6 -1.4394782777170363 2316 7 -0.1315349109791697 2316 8 -0.374327406912208 2316 9 0.01535773769045079 2316 10-11 -0.04894891969754411 2316 12-13 -0.8327372470855892 2316 14-15 -0.8504084870769901 2316 16-17 -1.3171433775943555 2316 18-19 -0.4130303335693526 2316 20-21 -4.033319022626635 2316 22-23 -3.7775524726943956 2316 24-25 -2.44584362931316 2316 26-27 -1.9030699757731284 2316 28-29 -1.423686684752667 2316 30-31 -0.4531597808147083 2316 32-33 -1.8228914313651572 2316 34-35 -2.535663516287336 2316 36-37 -4.2251411295764605 2316 38-39 -1.8328356794648855 2316 40-41 -1.7739090391694425 2316 42-43 -2.5381052386522214 2316 44-45 -0.4029428932351138 2316 46-47 -0.4543025374420715 2316 48-49 -0.2512368932245579 2316 50-51 0.025206624250458276 2316 52-53 -1.7151151510789404 2316 54-55 -0.5057805380099651 2316 56-57 0.5098381818003404 2316 58-59 0.3964231603663997 2316 60-61 -0.8887463986189594 2316 62-63 0.049907597495611355 2316 64-65 0.760205795568961 2316 66-67 2.2625403010744414 2316 68-69 5.040660076322329 2316 70-71 4.30515205000216 2316 72-73 2.5387657742488976 2316 74-75 1.2043998854450209 2316 76-77 -0.1264898908731631 2316 78-79 0.15214169032713087 2316 80-81 -0.547453405187742 2316 82-83 0.13047365376564457 2316 84-85 0.303902975006789 2316 86-87 -0.044892354211995666 2316 88-89 0.6250073449240041 2316 90-91 1.0983016335113014 2316 92-93 1.1888983357604879 2316 94-95 1.7220279607972024 2316 96-97 1.9880755076401861 2316 98-99 0.66664083243192 2316 100-101 0.012738874915235954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 46.0 5 140.0 6 414.0 7 868.0 8 1258.0 9 1723.0 10 2952.0 11 4202.0 12 5614.0 13 6129.0 14 5819.0 15 6664.0 16 7956.0 17 8725.0 18 7151.0 19 4699.0 20 4304.0 21 4279.0 22 4653.0 23 5424.0 24 6334.0 25 7450.0 26 9020.0 27 10835.0 28 13102.0 29 15260.0 30 17696.0 31 20401.0 32 23780.0 33 28417.0 34 35171.0 35 38924.0 36 29184.0 37 24820.0 38 11971.0 39 969.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.387305265731555 20.27176426190995 13.968247218841187 13.372683253517309 2 15.849772644525531 26.53933342723429 42.05504899238073 15.555844935859447 3 18.932685026503528 39.726329493975264 28.70245639206621 12.638529087454998 4 10.055132650485564 21.82748130139625 35.82320353911761 32.29418250900057 5 8.638180059729411 43.42684209966947 33.590619719208 14.34435812139312 6 19.39473649250063 37.16232912199594 25.744423631315343 17.69851075418809 7 21.540260119830485 38.20918523242066 26.080267825116575 14.170286822632287 8 25.83104167496978 32.20092197733583 25.24862832622587 16.71940802146852 9 28.113400555311998 18.603483320712606 25.22285547274587 28.060260651229523 10-11 21.91861623689769 31.46214446082925 29.03564359066332 17.58359571160974 12-13 24.471324379259496 31.294886612729666 31.254633135387188 12.979155872623648 14-15 20.4748050429769 30.906832463167405 34.34272581137991 14.275636682475787 16-17 14.282848518986938 33.84901318935924 34.89215227811374 16.975986013540084 18-19 14.073437373376393 29.543271562048723 42.239575514498696 14.143715550076191 20-21 16.990952931329957 28.08164946262272 42.42464097352304 12.502756632524278 22-23 15.81496685398483 29.56425278652372 42.09238372324738 12.528396636244072 24-25 14.429476704794547 30.237136821968036 42.880847050071075 12.45253942316634 26-27 14.052940981101997 30.468364940715404 42.71626423992826 12.762429838254343 28-29 13.381974442821893 31.09753423856644 42.620454512235796 12.900036806375875 30-31 13.580123900130598 31.350878101987107 42.55290599346622 12.516092004416086 32-33 13.378549838907894 31.878965024745078 42.09785099810675 12.644634138240276 34-35 13.65205942101578 32.250024247239466 41.49479913321101 12.60311719853374 36-37 13.677537918177055 32.37652490512144 41.0729055576093 12.873031619092199 38-39 13.762004686182678 32.85553950941359 40.54877805526079 12.83367774914295 40-41 13.847281297465239 32.69372467966367 40.646072571442254 12.812921451428846 42-43 13.456508238773498 33.31600161171713 40.00329790303899 13.224192246470379 44-45 13.557789479283016 33.70685069943088 39.376495931067495 13.358863890218606 46-47 13.903279682563321 33.76993287448273 38.441193366169166 13.885594076784782 48-49 13.98380362224409 34.294832584239785 37.616153825696124 14.105209967820004 50-51 14.03946880273178 34.75480594704782 37.05775114788235 14.14797410233805 52-53 13.908718922238794 35.127607926341284 36.639387630844986 14.324285520574934 54-55 13.922300750620423 35.07086829781832 36.524256590691884 14.482574360869375 56-57 14.022987589253322 35.21933094394233 35.98372343662998 14.773958030174366 58-59 13.96426322125286 35.09847013209959 36.1064237444427 14.83084290220485 60-61 13.91843182479463 35.122384149249804 36.483935212064765 14.475248813890799 62-63 14.1001182394282 34.85100503513969 36.525886463578274 14.522990261853835 64-65 14.058719823354277 35.128266105835976 36.19510211998783 14.617911950821917 66-67 14.26830227779163 35.208223039820346 35.691892043264836 14.83158263912318 68-69 15.250610265304813 34.77615575683604 35.11394295625362 14.859291021605534 70-71 15.17683853814368 34.757060482061654 34.982620867014475 15.083480112780192 72-73 14.261037720897587 35.28275024405446 34.996677130265816 15.459534904782135 74-75 13.544492232866343 35.431885091387045 35.63875145837463 15.384871217371984 76-77 13.389979139816377 35.41328408381276 35.74306099942867 15.453675776942188 78-79 13.144654171610174 35.279159154531 35.78097203160478 15.795214642254052 80-81 12.961629516655728 35.76019577156796 35.47442986313527 15.803744848641053 82-83 13.011438364353753 35.64438776188009 35.09306125702443 16.25111261674173 84-85 12.89492912465293 35.54103064843968 34.992228289027935 16.571811937879453 86-87 12.82827262968828 35.638902764345154 34.940376869201096 16.59244773676547 88-89 13.06923405985211 35.130632557571055 34.98808324985851 16.81205013271832 90-91 12.892966035830769 35.12876890680944 35.201307285058554 16.776957772301234 92-93 12.678453285294095 35.477695498181255 34.899258420816295 16.944592795708353 94-95 12.745305534590692 35.162268038767024 34.72366979608842 17.368756630553857 96-97 12.89609746486973 34.804394012811436 34.594331233593 17.70517728872583 98-99 12.873313057469982 34.5853612518419 34.5942634985298 17.947062192158317 100-101 12.54915767656906 34.349790083435195 34.92931923260881 18.171733007386937 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10349.0 1 6765.0 2 6792.5 3 9435.5 4 7000.0 5 5376.5 6 5912.5 7 8014.5 8 9053.0 9 8440.5 10 6934.5 11 5176.0 12 4437.5 13 4039.5 14 3730.5 15 3351.5 16 2917.5 17 2624.0 18 2521.5 19 2563.0 20 2707.0 21 2844.5 22 2961.5 23 3208.5 24 3479.0 25 3792.5 26 4182.0 27 4665.0 28 5198.5 29 5650.0 30 6299.0 31 6940.0 32 7648.0 33 8663.0 34 9685.0 35 10557.0 36 11450.0 37 12071.0 38 11736.5 39 11066.0 40 10358.5 41 9483.5 42 8769.0 43 8545.0 44 8448.5 45 8021.0 46 7616.5 47 7368.0 48 7609.0 49 7531.5 50 6919.5 51 6130.0 52 5205.0 53 4589.5 54 4043.5 55 3540.0 56 3142.0 57 2746.5 58 2318.5 59 1957.0 60 1682.0 61 1380.0 62 1025.5 63 705.0 64 470.0 65 340.5 66 273.5 67 210.5 68 149.0 69 118.5 70 110.0 71 93.0 72 80.0 73 83.0 74 83.5 75 52.5 76 27.5 77 17.5 78 12.5 79 14.5 80 12.0 81 8.5 82 5.0 83 3.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02205306019422635 2 0.021787360673813982 3 0.0 4 0.0 5 2.656995204123657E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.656995204123657E-4 18-19 9.299483214432798E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.642488010309141E-4 28-29 0.018997515709484145 30-31 0.0035869435255669363 32-33 0.007306736811340055 34-35 0.00890093393381425 36-37 0.0951204283076269 38-39 0.09724602447092583 40-41 0.11478219281814196 42-43 0.09791027327195674 44-45 0.09166633454226615 46-47 0.09379193070556507 48-49 0.09432332974638981 50-51 0.0920648838228847 52-53 0.06695627914391615 54-55 0.055664049526390605 56-57 0.03148539316886533 58-59 0.015410572183917207 60-61 0.007838135852164786 62-63 0.0026569952041236564 64-65 0.004915441127628765 66-67 0.3768947697049407 68-69 0.7325335777768921 70-71 0.8163617764669935 72-73 0.24882760086618044 74-75 0.1377652013338116 76-77 0.013284976020618282 78-79 0.007838135852164786 80-81 0.0021255961632989256 82-83 0.0 84-85 0.0 86-87 2.656995204123657E-4 88-89 0.0010627980816494628 90-91 0.0033212440051545705 92-93 0.0015941971224741939 94-95 0.19568769678370732 96-97 0.202728734074635 98-99 0.014746323382886294 100-101 0.006642488010309141 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 376365.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.96179504787747 #Duplication Level Percentage of deduplicated Percentage of total 1 86.78517704425973 51.170098220774044 2 8.260391459370757 9.740950164853123 3 2.0330875537273756 3.5962347497179232 4 0.8679374742608912 2.047006058869724 5 0.46505026573184954 1.3710099227521135 6 0.31823957729838054 1.1258386039674166 7 0.2209344398346825 0.9118683812385118 8 0.15821631768751956 0.7462974477377121 9 0.1317747899935952 0.6992710344071511 >10 0.6844469084300772 7.357141563719349 >50 0.043225543752401833 1.7397229671547803 >100 0.025665166602981473 2.644761208422435 >500 0.0013507982422621826 0.5750404313835569 >1k 0.0022513304037703043 3.1682975568473535 >5k 0.0013507982422621826 6.1547379135572475 >10k+ 9.005321615081219E-4 6.951723774597617 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13639 3.6238757589042554 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12546 3.33346618309354 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8625 2.2916583635566536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8057 2.14074103596243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6501 1.7273125822007893 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4990 1.3258406068577044 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2157 0.5731138655294727 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1977 0.5252879518552469 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1756 0.4665683578441141 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1054 0.2800472945146334 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 893 0.23726967172824254 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 652 0.1732360873088624 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 621 0.16499940217607909 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 392 0.10415421200164734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.6569952041236564E-4 9 0.0 0.0 0.0 0.0 2.6569952041236564E-4 10-11 0.0 0.0 0.0 0.0 2.6569952041236564E-4 12-13 0.0 0.0 0.0 0.0 2.6569952041236564E-4 14-15 0.0 0.0 0.0 0.0 2.6569952041236564E-4 16-17 0.0 0.0 0.0 2.6569952041236564E-4 2.6569952041236564E-4 18-19 0.0 0.0 0.0 2.6569952041236564E-4 2.6569952041236564E-4 20-21 0.0 0.0 0.0 3.9854928061854847E-4 2.6569952041236564E-4 22-23 0.0 0.0 0.0 0.002391295683711291 2.6569952041236564E-4 24-25 0.0 0.0 0.0 0.007838135852164786 2.6569952041236564E-4 26-27 0.0 0.0 0.0 0.011557929137937906 2.6569952041236564E-4 28-29 0.0 0.0 0.0 0.018598966428865597 2.6569952041236564E-4 30-31 0.0 0.0 0.0 0.03055544484742205 2.6569952041236564E-4 32-33 0.0 0.0 0.0 0.04530176823030835 2.6569952041236564E-4 34-35 0.0 0.0 0.0 0.06841762650618416 2.6569952041236564E-4 36-37 0.0 0.0 0.0 0.10282571439958552 2.6569952041236564E-4 38-39 0.0 0.0 0.0 0.15131587687484224 2.6569952041236564E-4 40-41 0.0 0.0 0.0 0.2083084240032947 2.6569952041236564E-4 42-43 0.0 0.0 0.0 0.265965219932778 2.6569952041236564E-4 44-45 0.0 0.0 0.0 0.3314601517144261 2.6569952041236564E-4 46-47 0.0 0.0 0.0 0.4093101111952493 2.6569952041236564E-4 48-49 0.0 0.0 0.0 0.48994991564040224 2.6569952041236564E-4 50-51 0.0 0.0 0.0 0.5723167669682356 2.6569952041236564E-4 52-53 0.0 0.0 0.0 0.6690313923983366 2.6569952041236564E-4 54-55 0.0 0.0 0.0 0.7674730647111181 2.6569952041236564E-4 56-57 0.0 0.0 0.0 0.8805282106465797 2.6569952041236564E-4 58-59 0.0 0.0 0.0 0.9878708168931756 2.6569952041236564E-4 60-61 0.0 0.0 0.0 1.0940177752979157 2.6569952041236564E-4 62-63 0.0 0.0 0.0 1.2041502265088413 2.6569952041236564E-4 64-65 0.0 0.0 0.0 1.3157440250820347 2.6569952041236564E-4 66-67 0.0 0.0 0.0 1.4367701566298672 2.6569952041236564E-4 68-69 0.0 0.0 0.0 1.5801150478923385 2.6569952041236564E-4 70-71 0.0 0.0 0.0 1.7638462662574894 2.6569952041236564E-4 72-73 0.0 0.0 0.0 1.9542199726329494 2.6569952041236564E-4 74-75 0.0 0.0 0.0 2.1630597956770687 2.6569952041236564E-4 76-77 0.0 0.0 0.0 2.3983367210022184 2.6569952041236564E-4 78-79 0.0 0.0 0.0 2.6595193495675744 2.6569952041236564E-4 80-81 0.0 0.0 0.0 2.9260159685411766 2.6569952041236564E-4 82-83 0.0 0.0 0.0 3.19596668128014 2.6569952041236564E-4 84-85 0.0 0.0 0.0 3.4671130418609595 2.6569952041236564E-4 86-87 0.0 0.0 0.0 3.7389236512428097 2.6569952041236564E-4 88-89 0.0 0.0 0.0 4.0412897054720816 2.6569952041236564E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 50 5.653419E-9 66.3581 1 TATAACG 65 9.094947E-12 65.62018 2 CGGTATC 35 2.8169164E-4 54.16268 2 ATCACGC 35 2.822469E-4 54.141098 3 TACCTGG 495 0.0 53.61558 2 ATAACGC 85 1.3278623E-10 50.16013 3 ACCTGGG 525 0.0 49.629333 3 GGTATCA 3940 0.0 48.601654 1 ACACGGG 50 2.8682074E-5 47.37345 3 TAACGCA 95 3.929017E-10 44.880116 4 GTACCTG 605 0.0 44.656574 1 TCACGCA 45 9.743895E-4 42.109737 4 GCGGTAT 50 0.0016314643 37.918915 1 TATCACG 50 0.0016325302 37.913876 2 TTCAACG 50 0.0016325302 37.913876 2 ATGGGCG 50 0.0016357312 37.89876 5 GTATAAC 115 2.5447662E-9 37.094593 1 GTATCAA 6145 0.0 33.013214 1 GCGTGGG 30 0.003691839 32.10697 64-65 GTATATG 320 0.0 31.105358 1 >>END_MODULE