FastQCFastQC Report
Fri 27 May 2016
SRR939542_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939542_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376506
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107302.8498881823928435No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75442.0036865282359377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66811.7744737135663176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50101.3306560851619895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42971.1412832730421294No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.9665184618571816No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.36015362304983184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11190.2972064190212108No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.2895040185282572No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.1681248107599879No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.150329609621095No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.10650560681635883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG3900.061.690042
ATCACGC503.8338476E-757.8250243
ATCGACG250.004741077357.8250243
CGGTATC451.2442273E-554.5558172
ACCTGGG4750.051.738183
GTACCTG4650.049.0979771
GGTATCA32300.049.0067671
TCACGCA707.650124E-847.6956754
TATCACG652.0574298E-645.3232962
CGCGGAG458.525537E-443.2677848
GTATCAA54850.039.9586751
GCGGTAT550.001865332736.897871
CCTGGGA3500.036.7938084
TATCGAC550.002190664935.7092632
ATATGGG2300.035.6168633
ATAACGC550.002401963835.0454673
ATGGGCG701.7689234E-434.7926035
GTAGTCC701.8846156E-434.4196553
ATGGGGT4000.032.879015
TACAACG752.5311403E-432.7334942
Produced by FastQC (version 0.11.5)