FastQCFastQC Report
Fri 27 May 2016
SRR939542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939542_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376506
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130333.461565021540161No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124493.3064546116130953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84832.2530849441974365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76322.0270593297317974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62451.658672106155015No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49671.3192352844310582No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.5694464364445719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.5293408338778134No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17250.45816002932224187No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.2695840172533771No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.23930561531555938No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.15909441018204226No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.15803201011404866No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.10252160656138282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC551.8189894E-1277.6493763
GCGTATC255.2865056E-575.954141
GAGCCGG200.002086742871.131326
TATAACG659.094947E-1265.7207952
TATCACG452.0543666E-763.2866972
TACCTGG3700.060.2934042
TAACGCA753.8198777E-1156.973184
ACCTGGG3850.055.4638443
CGTATCA451.5206164E-552.7389142
TTCAACG451.5206164E-552.7389142
ACGAGTA150.00996226347.93036770-71
ATCACGC601.4999114E-647.4524043
GGTATCA38650.046.3045121
GTACCTG5100.044.67891
TCACGCA652.5930858E-643.8255234
CCTGGGA3800.036.2329444
TATATGG2950.035.397642
GTATCAA59900.034.157171
ATATGGG3350.033.9957473
GAGTAAT2550.031.6475541
Produced by FastQC (version 0.11.5)