##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939542_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 376506 Sequences flagged as poor quality 0 Sequence length 101 %GC 30 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.876124152071945 31.0 31.0 31.0 28.0 34.0 2 30.939937212155982 31.0 31.0 33.0 28.0 34.0 3 30.893932633211687 31.0 30.0 34.0 27.0 34.0 4 34.836366485527456 35.0 35.0 37.0 32.0 37.0 5 34.523008929472574 35.0 35.0 37.0 30.0 37.0 6 34.66269329041237 35.0 35.0 37.0 31.0 37.0 7 34.54644547497251 35.0 35.0 37.0 30.0 37.0 8 34.49255257552336 35.0 35.0 37.0 30.0 37.0 9 35.92463068317636 37.0 35.0 39.0 30.0 39.0 10-11 35.70936319739924 37.0 35.0 39.0 30.0 39.0 12-13 35.690177580171365 37.0 35.0 39.0 30.0 39.0 14-15 36.2700952972861 38.0 34.5 40.0 29.5 41.0 16-17 36.274544097570825 38.0 34.5 40.0 29.5 41.0 18-19 36.44863162871243 38.0 35.0 40.0 30.0 41.0 20-21 36.35325333460821 38.0 35.0 40.0 30.0 41.0 22-23 35.959499981408 38.0 34.0 40.0 29.5 41.0 24-25 35.68301567571301 37.5 34.0 40.0 29.0 41.0 26-27 35.08955899773177 37.0 33.5 40.0 27.0 41.0 28-29 34.77357200150861 36.5 33.0 40.0 26.5 41.0 30-31 34.16513946656893 35.5 33.0 39.0 25.0 40.0 32-33 33.25507428832476 35.0 33.0 39.0 23.0 40.0 34-35 32.78251209808077 35.0 31.5 39.0 21.0 40.0 36-37 32.3145062761284 35.0 31.0 39.0 18.0 40.0 38-39 32.029003521856225 35.0 31.0 39.0 17.0 40.0 40-41 31.76119902471674 35.0 31.0 38.5 15.5 40.0 42-43 31.42710607533479 35.0 31.0 38.0 13.5 40.0 44-45 30.801325875284856 35.0 30.0 38.0 6.5 40.0 46-47 30.062299139987147 35.0 27.5 38.0 2.0 40.0 48-49 29.461364493527327 35.0 26.0 38.0 2.0 40.0 50-51 29.211218413517976 35.0 26.0 38.0 2.0 40.0 52-53 29.13404700057901 35.0 26.0 38.0 2.0 40.0 54-55 28.857933206907724 34.0 25.5 38.0 2.0 40.0 56-57 28.438358220054926 34.0 23.5 38.0 2.0 40.0 58-59 28.084828661429036 33.0 23.5 37.0 2.0 40.0 60-61 27.818220692366125 33.0 23.0 37.0 2.0 40.0 62-63 27.692392684313134 33.0 23.0 37.0 2.0 40.0 64-65 27.319386676440747 33.0 22.0 36.0 2.0 39.0 66-67 26.66729746670704 33.0 21.0 35.5 2.0 39.0 68-69 23.824973572798307 30.0 9.0 35.0 2.0 38.0 70-71 22.782368674071595 28.5 7.5 35.0 2.0 37.0 72-73 23.63338167253643 29.5 9.5 35.0 2.0 37.0 74-75 24.229778542705827 30.5 12.5 35.0 2.0 37.0 76-77 23.78823843444726 30.0 10.5 33.5 2.0 36.0 78-79 24.74337859157623 31.0 14.0 35.0 2.0 36.0 80-81 24.70891964537086 32.0 12.0 35.0 2.0 36.0 82-83 24.389074808900787 31.5 7.5 35.0 2.0 36.0 84-85 24.03547486627039 31.0 4.0 35.0 2.0 35.0 86-87 23.60153357449815 31.0 2.0 34.0 2.0 35.0 88-89 23.127437544156003 31.0 2.0 34.0 2.0 35.0 90-91 22.53998342655894 30.0 2.0 34.0 2.0 35.0 92-93 22.021772561393444 29.5 2.0 34.0 2.0 35.0 94-95 21.28882408248474 29.0 2.0 34.0 2.0 35.0 96-97 20.686449883932795 29.0 2.0 34.0 2.0 35.0 98-99 19.976495726495727 28.0 2.0 33.5 2.0 35.0 100-101 19.012058240771726 26.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.22464417063902076 1101 2 0.24196888386194004 1101 3 0.39610513399531655 1101 4 0.37175711094628383 1101 5 0.7184471125007974 1101 6 0.6067348940771282 1101 7 0.6847098446706781 1101 8 0.5314290757193163 1101 9 0.5142793147863784 1101 10-11 0.7981608585835929 1101 12-13 0.7402400335861756 1101 14-15 0.7615617102014198 1101 16-17 0.720821484243487 1101 18-19 0.6688305235390715 1101 20-21 0.7377156805653797 1101 22-23 1.1899441744361994 1101 24-25 1.0868534756899564 1101 26-27 0.9592778106832895 1101 28-29 1.2227977728292743 1101 30-31 1.0601130325467523 1101 32-33 1.6540514707078557 1101 34-35 1.389946248777953 1101 36-37 1.1634645759076747 1101 38-39 1.453569584095142 1101 40-41 1.1701588483029184 1101 42-43 1.3037963135259893 1101 44-45 1.4144207701277232 1101 46-47 1.5151069306748006 1101 48-49 1.9333411042791937 1101 50-51 1.3839266925155265 1101 52-53 0.9446995384056329 1101 54-55 1.1397739736545809 1101 56-57 1.2729516355812436 1101 58-59 1.6044812620147795 1101 60-61 1.5650755369117668 1101 62-63 0.7092750719917156 1101 64-65 0.4109580108237587 1101 66-67 0.6205398033407405 1101 68-69 -5.480480875685178 1101 70-71 -5.82692549417504 1101 72-73 -3.3489949084294324 1101 74-75 -1.413983690521949 1101 76-77 0.8235595730371905 1101 78-79 0.7172690505673103 1101 80-81 0.9497270086332357 1101 82-83 0.5418049248047332 1101 84-85 0.7867480374633651 1101 86-87 0.5583785737218356 1101 88-89 1.0943370969170019 1101 90-91 0.9218043287906674 1101 92-93 1.0047896962358998 1101 94-95 1.0680159268618432 1101 96-97 0.9127846350551998 1101 98-99 0.6584709453920681 1101 100-101 0.3318462344610289 1102 1 0.05955018483065189 1102 2 0.2824810427602138 1102 3 0.5821565299058449 1102 4 0.6466337217565297 1102 5 0.5513053912028454 1102 6 0.6057897853896321 1102 7 0.3186152437915766 1102 8 0.5156172573602049 1102 9 0.4797678459674657 1102 10-11 0.46601891225977 1102 12-13 0.4390841506596388 1102 14-15 0.47655893488225587 1102 16-17 0.5046053693902834 1102 18-19 0.3174826185299935 1102 20-21 0.323274269803143 1102 22-23 0.5323510512270317 1102 24-25 0.6544662511591355 1102 26-27 0.9775161580932448 1102 28-29 0.277257785730761 1102 30-31 0.4951893163193901 1102 32-33 0.7031671190074036 1102 34-35 1.005508288412507 1102 36-37 0.907115095717451 1102 38-39 1.7873579697594977 1102 40-41 1.8476555104190595 1102 42-43 1.1594384623730996 1102 44-45 1.2753510271072805 1102 46-47 1.474872303692699 1102 48-49 0.9722180751308436 1102 50-51 0.9479803086813838 1102 52-53 0.921214337607541 1102 54-55 0.91229156317738 1102 56-57 0.43416767542582235 1102 58-59 1.4094625848669082 1102 60-61 1.4257995201698428 1102 62-63 1.2004840610254597 1102 64-65 1.0567522771643887 1102 66-67 1.251231082837883 1102 68-69 -5.815953205003048 1102 70-71 -6.147129817672241 1102 72-73 -0.3786645704405629 1102 74-75 0.6603011022292158 1102 76-77 1.3715350902216663 1102 78-79 1.4092723809503056 1102 80-81 1.398706141233486 1102 82-83 1.1006004362885555 1102 84-85 1.4351301014007198 1102 86-87 0.7620457454465814 1102 88-89 0.4533771365215564 1102 90-91 0.6091571243621594 1102 92-93 0.5751215343972902 1102 94-95 0.34314756700045734 1102 96-97 0.13799053373354653 1102 98-99 -0.4026160796158038 1102 100-101 -0.4619700544121912 1103 1 0.2757939759435537 1103 2 0.1852867884978977 1103 3 0.2644763043722165 1103 4 0.1699791372485393 1103 5 0.41395809626392577 1103 6 0.3615288107621666 1103 7 0.3622721104850939 1103 8 0.4358078869749207 1103 9 0.2973004006727962 1103 10-11 0.28798159181407357 1103 12-13 0.4749950111778247 1103 14-15 0.35462981687984296 1103 16-17 0.33077666755110613 1103 18-19 0.4957682944596442 1103 20-21 0.49187408185844816 1103 22-23 0.6913254102013937 1103 24-25 0.5960960001187274 1103 26-27 0.5310065594894056 1103 28-29 0.04587102240682128 1103 30-31 0.26731309132274106 1103 32-33 0.48367456967191913 1103 34-35 0.3014406283239097 1103 36-37 0.5839039264329884 1103 38-39 0.6645092652354023 1103 40-41 0.748360303008667 1103 42-43 0.7333275750056707 1103 44-45 0.8417626259796087 1103 46-47 0.9406193838376851 1103 48-49 0.9540008647670355 1103 50-51 0.8278433775496872 1103 52-53 0.7692274937358796 1103 54-55 0.4087071894359404 1103 56-57 0.6717834627798318 1103 58-59 0.8309084165221634 1103 60-61 0.9066962848362401 1103 62-63 0.3892140919020868 1103 64-65 0.266585811867035 1103 66-67 0.5497073883822523 1103 68-69 -6.058932128348463 1103 70-71 -6.93854477269976 1103 72-73 -0.2764763572575859 1103 74-75 1.174007640019525 1103 76-77 1.0939474945712426 1103 78-79 1.3004591174249995 1103 80-81 1.0794015365885699 1103 82-83 0.6897734796500785 1103 84-85 0.8020518358617217 1103 86-87 0.835793899561228 1103 88-89 0.6581898630620309 1103 90-91 0.3650303959422345 1103 92-93 0.7618244475399933 1103 94-95 1.0442483859023248 1103 96-97 0.4744920775649355 1103 98-99 0.14982720136150718 1103 100-101 -0.4154537434979737 1104 1 0.31371121641834065 1104 2 0.248864728034917 1104 3 0.32286701851740673 1104 4 0.3489810390125925 1104 5 0.21975519771320506 1104 6 0.5276708528632881 1104 7 0.3931790552027792 1104 8 0.4385293769086829 1104 9 0.3862701691822039 1104 10-11 0.5360601543427563 1104 12-13 0.26230893340223815 1104 14-15 0.7468686522133012 1104 16-17 0.6821505281321265 1104 18-19 0.6506932075687075 1104 20-21 0.5895099924099654 1104 22-23 0.4645761151656487 1104 24-25 0.43807252316941714 1104 26-27 0.6061131238542856 1104 28-29 0.6806569100060003 1104 30-31 0.7431606878864514 1104 32-33 0.8986853900535863 1104 34-35 0.6898824933642445 1104 36-37 1.3180897746853262 1104 38-39 1.4055808109105286 1104 40-41 1.3364578831778573 1104 42-43 1.2250876904230772 1104 44-45 1.2184791319800077 1104 46-47 1.1181818469500158 1104 48-49 1.1969807322993304 1104 50-51 1.2853586244511241 1104 52-53 1.0660436784037266 1104 54-55 1.01025155300076 1104 56-57 0.9348171043300972 1104 58-59 0.8937256504391407 1104 60-61 1.1783403270685184 1104 62-63 1.166258488255945 1104 64-65 1.3518340248646084 1104 66-67 1.4414970139314676 1104 68-69 -4.695223032670057 1104 70-71 -3.289830102885343 1104 72-73 1.6918855025365005 1104 74-75 1.7559244870258865 1104 76-77 1.9304121807801735 1104 78-79 1.2771002086440966 1104 80-81 1.3188598617246434 1104 82-83 0.9067057375030529 1104 84-85 1.353255193458942 1104 86-87 1.2819805347504207 1104 88-89 1.7466612382341715 1104 90-91 1.4097054566019516 1104 92-93 1.236511312252432 1104 94-95 1.5879889590546625 1104 96-97 0.9394996190430653 1104 98-99 1.0636805687954372 1104 100-101 1.126352276568916 1105 1 -0.012623353853086883 1105 2 0.1795396620611136 1105 3 0.18277869164012017 1105 4 0.13319752805313811 1105 5 0.5469377602780767 1105 6 -0.13847118923783341 1105 7 0.25855858263363274 1105 8 0.5131730505469818 1105 9 0.16670800456315504 1105 10-11 0.24956426201742943 1105 12-13 0.4168606168365301 1105 14-15 0.45476244287453227 1105 16-17 0.38771742794013875 1105 18-19 0.18016263575053415 1105 20-21 0.4884700146612815 1105 22-23 0.19415476475487736 1105 24-25 0.4680677065731942 1105 26-27 0.7822886107714595 1105 28-29 0.7418480339010713 1105 30-31 0.6725297137807473 1105 32-33 0.38022629380985506 1105 34-35 0.46735574768730714 1105 36-37 0.4290851819590671 1105 38-39 0.5826348178437115 1105 40-41 0.9531348388176895 1105 42-43 0.8789067085158386 1105 44-45 0.7479960477302754 1105 46-47 0.90202521938145 1105 48-49 0.9233200513276003 1105 50-51 0.5903102210510163 1105 52-53 0.9543291736651476 1105 54-55 0.6271422026985363 1105 56-57 0.8508285556180262 1105 58-59 1.0263991327025366 1105 60-61 1.236227672988317 1105 62-63 0.7944749230249855 1105 64-65 0.8744991628838328 1105 66-67 0.6558966014680152 1105 68-69 -2.608622667694174 1105 70-71 -2.4966791670410515 1105 72-73 1.086432572892722 1105 74-75 1.3228582147321681 1105 76-77 1.3877140728205788 1105 78-79 0.8037305586274783 1105 80-81 0.8893042775257918 1105 82-83 0.9545833823910179 1105 84-85 0.22309516035332067 1105 86-87 0.5865454468645019 1105 88-89 0.9308689081548671 1105 90-91 1.094252323440024 1105 92-93 0.9996670646929537 1105 94-95 1.3112687218881796 1105 96-97 1.4263930501555642 1105 98-99 2.0845310033066866 1105 100-101 1.8299043466877016 1106 1 0.10396025410983256 1106 2 0.049311652522760596 1106 3 0.1760536159495274 1106 4 0.291035858305257 1106 5 0.4301197124255438 1106 6 0.11852181775517323 1106 7 0.017594565807549145 1106 8 0.1362740874411159 1106 9 0.24357079694318884 1106 10-11 0.4293763082087878 1106 12-13 0.12786991405272374 1106 14-15 0.14930736155738344 1106 16-17 0.43007736685181186 1106 18-19 0.29994467363601984 1106 20-21 0.5930201580045278 1106 22-23 0.3543207481967272 1106 24-25 0.10563368465641787 1106 26-27 0.358531812014661 1106 28-29 0.0501833767191755 1106 30-31 0.26943041844007354 1106 32-33 0.5445720055693641 1106 34-35 0.38675531363859506 1106 36-37 -0.22190415437509614 1106 38-39 -0.5474759717498365 1106 40-41 -0.5718999922516268 1106 42-43 -0.35589153921344163 1106 44-45 -0.3141037298867424 1106 46-47 -0.3534171351988391 1106 48-49 0.004583615349787351 1106 50-51 -0.4263718516655395 1106 52-53 -0.6920584425500564 1106 54-55 -0.806638575909826 1106 56-57 -0.6714566304602592 1106 58-59 -0.6378561522424064 1106 60-61 -0.0042205760806197645 1106 62-63 -0.2725380448500516 1106 64-65 1.5224543346903374E-4 1106 66-67 -0.1595389208640583 1106 68-69 -2.2754433948597317 1106 70-71 -2.3365634207184094 1106 72-73 1.072707791926561 1106 74-75 1.1441513851632727 1106 76-77 0.9963367719605181 1106 78-79 0.3741964911623725 1106 80-81 0.06998040216743462 1106 82-83 0.04816508804168862 1106 84-85 0.16094849475837947 1106 86-87 0.4930005817679124 1106 88-89 -0.0474822426100765 1106 90-91 0.04383769974954177 1106 92-93 -0.028288217572672636 1106 94-95 0.5685296601836001 1106 96-97 0.22552160359446205 1106 98-99 -0.22795057641626926 1106 100-101 -0.0743504023946322 1107 1 0.28796349899390705 1107 2 0.31110052571059654 1107 3 0.2973180740015522 1107 4 -0.10193530409595297 1107 5 0.4987594779565043 1107 6 0.42151286815554556 1107 7 -0.14119540645392448 1107 8 0.30042858579250975 1107 9 0.20106019873310998 1107 10-11 0.15457518820041827 1107 12-13 0.18131891180225068 1107 14-15 0.03219059806756519 1107 16-17 0.2658836158704858 1107 18-19 0.47684973460844304 1107 20-21 0.54057210231813 1107 22-23 0.5854598595171865 1107 24-25 0.6223880155299142 1107 26-27 1.0660670085394912 1107 28-29 0.8183965970049272 1107 30-31 0.6061067674221192 1107 32-33 0.4070504166229014 1107 34-35 0.25863472955946065 1107 36-37 0.38373121486130657 1107 38-39 0.05042662939110443 1107 40-41 0.005394978178060228 1107 42-43 -0.43794916207417245 1107 44-45 -0.18850839833286415 1107 46-47 -0.1985197154050411 1107 48-49 0.018714562123221867 1107 50-51 0.014232377151124354 1107 52-53 -0.15750958611000598 1107 54-55 -0.3421098691531448 1107 56-57 -0.2566740900300495 1107 58-59 0.2978872925683973 1107 60-61 -0.25224486037433635 1107 62-63 -0.49238548296174045 1107 64-65 -0.24074114442365513 1107 66-67 -0.4872329596979945 1107 68-69 -3.0118881533252164 1107 70-71 -2.63566846075609 1107 72-73 0.21570512274772824 1107 74-75 0.5104896381595552 1107 76-77 -0.08152081949810963 1107 78-79 -0.1567084127995244 1107 80-81 -0.5864347859835028 1107 82-83 -0.6451541143448729 1107 84-85 -0.6178498513974766 1107 86-87 -0.5148172336923906 1107 88-89 -0.7624558125059231 1107 90-91 -0.3885234767826198 1107 92-93 -0.3558187037819138 1107 94-95 0.15328917240425355 1107 96-97 0.4932179975861892 1107 98-99 0.8188254742457914 1107 100-101 1.1405446622413642 1108 1 -0.014844068182860326 1108 2 -0.1547052607964794 1108 3 0.05906982454713372 1108 4 0.12613099572597974 1108 5 -0.22480159365855457 1108 6 -0.12137717214381638 1108 7 0.025698864609523753 1108 8 0.4999104510774828 1108 9 0.3222059860434996 1108 10-11 0.14328868781884552 1108 12-13 0.17003575854415942 1108 14-15 0.26006611185101747 1108 16-17 0.11201300227580901 1108 18-19 0.20060828650893825 1108 20-21 -0.02886732144109061 1108 22-23 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-0.0665391650048619 2316 100-101 0.16793733989311121 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 47.0 5 188.0 6 461.0 7 847.0 8 1385.0 9 2014.0 10 3114.0 11 4577.0 12 5835.0 13 6240.0 14 6037.0 15 7198.0 16 8167.0 17 9068.0 18 7023.0 19 5075.0 20 4613.0 21 4890.0 22 5084.0 23 5789.0 24 6545.0 25 7955.0 26 9469.0 27 11456.0 28 13670.0 29 15816.0 30 18490.0 31 21102.0 32 24556.0 33 28833.0 34 34402.0 35 36578.0 36 27017.0 37 22070.0 38 10121.0 39 758.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.03879677915536 20.5502851844866 13.97550096566894 13.435417070689093 2 15.598550578334121 26.825192733764403 41.90252533034381 15.67373135755766 3 18.84849016061217 39.8930137557537 28.66662948177542 12.591866601858715 4 9.997687012976655 21.82343135612822 35.87177012631036 32.30711150458477 5 8.682730155694731 43.62798999219136 33.48180374283544 14.207476109278472 6 19.464497245727824 37.33565998948224 25.68458404381338 17.515258720976558 7 21.419844570870055 38.28518005025152 26.106622470823837 14.188352908054586 8 26.025614465639325 32.035611650279144 25.203582413029274 16.735191471052254 9 28.07524979681599 18.725597998438275 25.23120481479711 27.967947389948634 10-11 21.73379465145468 31.572886713639654 29.079643784490944 17.61367485041472 12-13 24.335999957503997 31.346246806159794 31.263379600856293 13.054373635479912 14-15 20.311761299952725 30.93350437974428 34.38271900049401 14.37201531980898 16-17 14.357198917941218 33.86599628955298 34.78842882355988 16.988375968945924 18-19 14.166175306635221 29.60391069465028 42.17954029949058 14.050373699223917 20-21 16.99136268744721 28.262630608808358 42.16108109830919 12.584925605435238 22-23 15.716636277557699 29.70713575233297 41.95755440491573 12.618673565193603 24-25 14.513136593211179 30.30560192729354 42.72370988069957 12.457551598795707 26-27 14.115059702543084 30.563771028031177 42.481038748550766 12.840130520874974 28-29 13.366763966098363 31.155694141582625 42.48713814837145 12.99040374394756 30-31 13.592231720386556 31.456379787280664 42.40455982714728 12.546828665185503 32-33 13.505283469387482 31.898954726968736 41.91981769142201 12.675944112221766 34-35 13.57743508563172 32.29635905826357 41.35130253707875 12.774903319025965 36-37 13.74372687449496 32.45983604814358 40.833661166163395 12.96277591119806 38-39 13.8341355260377 32.8428459962808 40.437823086933236 12.885195390748267 40-41 13.819141556297993 32.85092720318733 40.567033189010175 12.762898051504495 42-43 13.423651866972866 33.40097920387854 39.88116122310113 13.294207706047462 44-45 13.632672697576073 33.693752599816335 39.18296992386276 13.490604778744833 46-47 13.919565800979711 33.83940271055436 38.282792843796265 13.95823864466966 48-49 14.018907691449014 34.256037889801696 37.63358839491932 14.091466023829962 50-51 14.032464900565444 34.73358981003449 37.0103853130544 14.223559976345673 52-53 13.82438330864132 35.07032241348384 36.723646534370616 14.381647743504228 54-55 13.89898243744694 34.94242580854306 36.484779076394915 14.673812677615086 56-57 14.090334887044861 35.21182326210347 35.97442154243837 14.723420308413287 58-59 13.977583371633056 35.20218268569167 36.0060783652173 14.81415557745798 60-61 13.976947924303762 35.00924420643269 36.431736316980654 14.582071552282894 62-63 13.997152707318497 34.94082305882103 36.464071522292286 14.597952711568189 64-65 14.193392442586287 35.09209037447075 36.05323551442581 14.661281668517152 66-67 13.865849620400624 35.38858236355633 35.866522513321556 14.879045502721494 68-69 15.220280159678717 34.85619022409031 34.98132357934872 14.942206036882254 70-71 15.179848216246326 34.80515550604403 34.75802434752803 15.256971930181615 72-73 14.470772112954181 35.0337575399834 34.92089929500239 15.57457105206003 74-75 13.722069497905338 35.36306829279311 35.440530302527016 15.474331906774538 76-77 13.323497679220209 35.3401083163604 35.916545051331205 15.419848953088186 78-79 13.23403820126523 35.23157574574283 35.767100053525866 15.767285999466068 80-81 13.01290998601562 35.798683370009684 35.24647966819968 15.941926975775017 82-83 13.139424781640397 35.5868053296078 34.971401100396946 16.302368788354862 84-85 13.015082116994305 35.44981414614129 34.9623046675281 16.572799069336305 86-87 12.876262619555431 35.50925766071092 34.937144587369424 16.67733513236423 88-89 13.111910864397553 35.057037755142694 34.980810347804145 16.850241032655607 90-91 13.014619168534788 35.02650786736504 35.08706692306875 16.871806041031416 92-93 12.676666126586046 35.442392433204866 34.713283592793374 17.167657847415715 94-95 12.694601070297582 35.19756119925422 34.638757955138935 17.469079775309268 96-97 12.833000548662948 34.87875611599771 34.50505420869651 17.783189126642835 98-99 13.024369640751013 34.48713248966668 34.447950867574086 18.040547002008225 100-101 12.623071661475732 34.2187365107615 34.86170417643426 18.2964876513285 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10060.0 1 6815.5 2 6990.5 3 9296.0 4 6740.5 5 5326.0 6 5951.0 7 7871.5 8 9021.5 9 8463.5 10 6857.5 11 5113.0 12 4355.5 13 3960.0 14 3688.0 15 3284.0 16 2852.5 17 2656.5 18 2575.5 19 2603.0 20 2678.5 21 2808.0 22 2984.0 23 3218.0 24 3519.5 25 3844.5 26 4232.0 27 4723.5 28 5260.0 29 5746.0 30 6324.0 31 6922.5 32 7627.5 33 8675.0 34 9864.0 35 10744.0 36 11528.5 37 12066.5 38 11764.0 39 11109.0 40 10406.0 41 9564.5 42 8863.0 43 8586.5 44 8351.5 45 7972.5 46 7510.0 47 7298.0 48 7627.0 49 7435.0 50 6835.5 51 6115.0 52 5212.5 53 4585.5 54 4066.0 55 3565.5 56 3139.5 57 2767.0 58 2398.5 59 2033.0 60 1701.5 61 1408.5 62 1050.0 63 683.5 64 447.5 65 349.0 66 283.0 67 219.5 68 169.0 69 140.0 70 117.0 71 98.0 72 90.5 73 90.0 74 78.0 75 57.5 76 35.0 77 21.5 78 16.0 79 7.0 80 6.0 81 6.0 82 4.0 83 3.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02204480141086729 2 0.021248001359872086 3 0.0023904001529856094 4 0.09800640627241002 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012881600824422452 12-13 0.0 14-15 0.0 16-17 3.984000254976016E-4 18-19 0.0 20-21 0.0 22-23 1.3280000849920056E-4 24-25 0.005179200331468821 26-27 0.0038512002464768155 28-29 0.0013280000849920054 30-31 1.3280000849920056E-4 32-33 3.984000254976016E-4 34-35 0.0026560001699840107 36-37 0.07914880506552352 38-39 0.09282720594094118 40-41 0.07091520453857308 42-43 0.07489920479354911 44-45 0.0737040047170563 46-47 0.0725088046405635 48-49 0.06825920436858908 50-51 0.06732960430909468 52-53 0.054049603459174624 54-55 0.04395680281323538 56-57 0.025364801623347304 58-59 0.023638401512857697 60-61 0.014342400917913658 62-63 0.002124800135987209 64-65 0.0074368004759552304 66-67 0.13744800879667257 68-69 0.6653280425809948 70-71 0.8180480523550754 72-73 0.33372642135849095 74-75 0.05112800327219221 76-77 0.17596001126144073 78-79 0.013944000892416058 80-81 0.0038512002464768155 82-83 0.001460800093491206 84-85 1.3280000849920056E-4 86-87 0.0018592001189888076 88-89 2.656000169984011E-4 90-91 0.0037184002379776152 92-93 0.00504640032296962 94-95 0.06786080434309148 96-97 0.03625440232028175 98-99 0.014342400917913658 100-101 0.012881600824422452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 376506.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.59767826828058 #Duplication Level Percentage of deduplicated Percentage of total 1 87.03332069873136 51.86983845623075 2 8.120897915912193 9.679733224841703 3 2.021783661963974 3.614808366413852 4 0.8197123961093664 1.9541182262338956 5 0.45921659519366254 1.3684121447903572 6 0.2861232439892988 1.0231368624210586 7 0.21151871923123813 0.8824217203523473 8 0.15383671743338856 0.7334648953154785 9 0.11586842620289241 0.6214940267662793 >10 0.7089492144306093 7.687864404528182 >50 0.0410848168427934 1.726234585615052 >100 0.021882130709743056 2.39559628509376 >500 0.0013397222883516156 0.5575796264050025 >1k 0.002232870480586026 3.152254460687756 >5k 0.0013397222883516156 5.951064652227139 >10k+ 8.931481922344105E-4 6.781978062077457 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13033 3.461565021540161 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12449 3.3064546116130953 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8483 2.2530849441974365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7632 2.0270593297317974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6245 1.658672106155015 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4967 1.3192352844310582 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2144 0.5694464364445719 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1993 0.5293408338778134 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1725 0.45816002932224187 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1015 0.2695840172533771 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 901 0.23930561531555938 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 599 0.15909441018204226 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 595 0.15803201011404866 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 386 0.10252160656138282 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.6560001699840106E-4 9 0.0 0.0 0.0 0.0 2.6560001699840106E-4 10-11 0.0 0.0 0.0 0.0 2.6560001699840106E-4 12-13 0.0 0.0 0.0 0.0 2.6560001699840106E-4 14-15 0.0 0.0 0.0 0.0 2.6560001699840106E-4 16-17 0.0 0.0 0.0 2.6560001699840106E-4 2.6560001699840106E-4 18-19 0.0 0.0 0.0 2.6560001699840106E-4 2.6560001699840106E-4 20-21 0.0 0.0 0.0 0.0010624000679936043 2.6560001699840106E-4 22-23 0.0 0.0 0.0 0.003585600229478415 2.6560001699840106E-4 24-25 0.0 0.0 0.0 0.008897600569446436 2.6560001699840106E-4 26-27 0.0 0.0 0.0 0.013147200841420854 2.6560001699840106E-4 28-29 0.0 0.0 0.0 0.01938880124088328 2.6560001699840106E-4 30-31 0.0 0.0 0.0 0.030544001954816123 2.6560001699840106E-4 32-33 0.0 0.0 0.0 0.0474096030342146 2.6560001699840106E-4 34-35 0.0 0.0 0.0 0.0718448045980675 2.6560001699840106E-4 36-37 0.0 0.0 0.0 0.10637280680785963 2.6560001699840106E-4 38-39 0.0 0.0 0.0 0.15444640988457023 2.6560001699840106E-4 40-41 0.0 0.0 0.0 0.20690241324175446 2.6560001699840106E-4 42-43 0.0 0.0 0.0 0.2641392169049099 2.6560001699840106E-4 44-45 0.0 0.0 0.0 0.3339920213754894 2.6560001699840106E-4 46-47 0.0 0.0 0.0 0.4151328265685009 2.6560001699840106E-4 48-49 0.0 0.0 0.0 0.49149283145554123 2.6560001699840106E-4 50-51 0.0 0.0 0.0 0.577414436954524 2.6560001699840106E-4 52-53 0.0 0.0 0.0 0.6630704424365084 2.6560001699840106E-4 54-55 0.0 0.0 0.0 0.7642640489128991 2.6560001699840106E-4 56-57 0.0 0.0 0.0 0.880065656324202 2.6560001699840106E-4 58-59 0.0 0.0 0.0 0.9875008632000553 2.6560001699840106E-4 60-61 0.0 0.0 0.0 1.101177670475371 2.6560001699840106E-4 62-63 0.0 0.0 0.0 1.2156512778016817 2.6560001699840106E-4 64-65 0.0 0.0 0.0 1.3278672849835063 2.6560001699840106E-4 66-67 0.0 0.0 0.0 1.4545584930917435 2.6560001699840106E-4 68-69 0.0 0.0 0.0 1.59798250227088 2.6560001699840106E-4 70-71 0.0 0.0 0.0 1.768099313158356 2.6560001699840106E-4 72-73 0.0 0.0 0.0 1.9472465246237776 2.6560001699840106E-4 74-75 0.0 0.0 0.0 2.148704137517065 2.6560001699840106E-4 76-77 0.0 0.0 0.0 2.3842913525946465 2.6560001699840106E-4 78-79 0.0 0.0 0.0 2.6427201691340905 2.6560001699840106E-4 80-81 0.0 0.0 0.0 2.9233265870929017 2.6560001699840106E-4 82-83 0.0 0.0 0.0 3.2026050049667205 2.6560001699840106E-4 84-85 0.0 0.0 0.0 3.4875938232060046 2.6560001699840106E-4 86-87 0.0 0.0 0.0 3.791307442643676 2.6560001699840106E-4 88-89 0.0 0.0 0.0 4.105910662778283 2.6560001699840106E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 55 1.8189894E-12 77.649376 3 GCGTATC 25 5.2865056E-5 75.95414 1 GAGCCGG 20 0.0020867428 71.13132 6 TATAACG 65 9.094947E-12 65.720795 2 TATCACG 45 2.0543666E-7 63.286697 2 TACCTGG 370 0.0 60.293404 2 TAACGCA 75 3.8198777E-11 56.97318 4 ACCTGGG 385 0.0 55.463844 3 CGTATCA 45 1.5206164E-5 52.738914 2 TTCAACG 45 1.5206164E-5 52.738914 2 ACGAGTA 15 0.009962263 47.930367 70-71 ATCACGC 60 1.4999114E-6 47.452404 3 GGTATCA 3865 0.0 46.304512 1 GTACCTG 510 0.0 44.6789 1 TCACGCA 65 2.5930858E-6 43.825523 4 CCTGGGA 380 0.0 36.232944 4 TATATGG 295 0.0 35.39764 2 GTATCAA 5990 0.0 34.15717 1 ATATGGG 335 0.0 33.995747 3 GAGTAAT 255 0.0 31.647554 1 >>END_MODULE