##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939541_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547813 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.944218191244094 31.0 31.0 31.0 30.0 34.0 2 31.039926033153648 31.0 31.0 33.0 28.0 34.0 3 31.023915095114575 31.0 31.0 34.0 27.0 34.0 4 34.93800439200968 35.0 35.0 37.0 32.0 37.0 5 34.64924709709335 35.0 35.0 37.0 32.0 37.0 6 34.68190057556137 35.0 35.0 37.0 31.0 37.0 7 34.61326949159658 35.0 35.0 37.0 30.0 37.0 8 34.68079618409932 35.0 35.0 37.0 31.0 37.0 9 35.96268252122531 37.0 35.0 39.0 30.0 39.0 10-11 35.782099000936455 37.0 35.0 39.0 30.0 39.0 12-13 35.74772778302085 37.0 35.0 39.0 30.0 39.0 14-15 36.37235242683178 38.0 35.0 40.0 30.0 41.0 16-17 36.433772108365446 38.0 34.5 40.0 30.5 41.0 18-19 36.5667590948006 38.0 35.0 40.0 30.0 41.0 20-21 36.453459483436866 38.0 35.0 40.0 30.0 41.0 22-23 36.14686854820897 38.0 34.0 40.0 30.0 41.0 24-25 35.838324391717606 38.0 34.0 40.0 29.5 41.0 26-27 35.22586448295313 37.5 33.5 40.0 27.0 41.0 28-29 34.86747576271465 37.0 33.0 40.0 26.5 41.0 30-31 34.48427474338871 36.5 33.0 39.0 25.5 40.5 32-33 33.93163543033845 36.0 33.0 39.0 24.5 40.0 34-35 33.462032664431106 36.0 33.0 39.0 22.5 41.0 36-37 33.07812611237777 36.0 32.0 39.0 20.5 41.0 38-39 32.89723774353657 35.5 32.0 39.0 19.5 41.0 40-41 32.588062897375565 35.0 31.5 39.0 17.0 41.0 42-43 32.24670371093785 35.0 31.0 39.0 15.5 41.0 44-45 31.92977530653709 35.0 31.0 39.0 12.0 41.0 46-47 31.353639836951658 35.0 30.0 39.0 5.0 41.0 48-49 30.879917051986716 35.0 29.5 39.0 2.0 40.0 50-51 30.582122914206124 35.0 29.0 39.0 2.0 40.0 52-53 30.10810258245058 35.0 28.0 39.0 2.0 40.0 54-55 29.942607240061847 35.0 28.0 39.0 2.0 40.0 56-57 29.65279575329538 35.0 27.0 38.0 2.0 40.0 58-59 29.322207578133415 34.5 26.5 38.0 2.0 40.0 60-61 29.040505610491174 34.0 26.0 38.0 2.0 40.0 62-63 28.605208346643835 34.0 26.0 38.0 2.0 40.0 64-65 28.126271191081628 33.5 25.5 37.0 2.0 40.0 66-67 26.65470333854801 32.5 18.5 36.5 2.0 39.0 68-69 23.52312833028789 28.5 10.5 35.0 2.0 38.0 70-71 22.99966229351987 28.5 9.5 34.0 2.0 37.0 72-73 24.534964486056374 30.0 14.0 35.0 2.0 37.0 74-75 25.02776403626785 31.0 18.0 35.0 2.0 37.0 76-77 24.88608612793052 30.5 20.0 34.0 2.0 36.0 78-79 25.6000396120574 32.0 20.0 35.0 2.0 36.0 80-81 25.49537981026372 32.0 20.0 35.0 2.0 36.0 82-83 25.142111450440204 32.0 18.5 35.0 2.0 36.0 84-85 24.825964334544818 32.0 17.5 35.0 2.0 35.0 86-87 24.311051398926274 31.0 9.5 34.0 2.0 35.0 88-89 23.73646572826859 31.0 4.5 34.0 2.0 35.0 90-91 23.243449863365782 31.0 2.0 34.0 2.0 35.0 92-93 22.790964252399995 30.5 2.0 34.0 2.0 35.0 94-95 21.650790506979572 29.0 2.0 34.0 2.0 35.0 96-97 20.841344582914243 29.0 2.0 34.0 2.0 35.0 98-99 20.51212092447605 29.0 2.0 34.0 2.0 35.0 100-101 19.505746486483524 27.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43396482716392626 1101 2 0.23928006231574628 1101 3 0.44089321544374727 1101 4 0.25779019135678993 1101 5 0.6809560657941987 1101 6 0.7189717460622873 1101 7 0.4421909118476819 1101 8 0.7481174573059448 1101 9 1.0111789281837176 1101 10-11 0.8588033941936359 1101 12-13 0.6346443240297646 1101 14-15 0.7885312785929628 1101 16-17 0.9266826856584203 1101 18-19 0.8013603090650534 1101 20-21 0.83565513565798 1101 22-23 0.7745677752788325 1101 24-25 0.8607717268320485 1101 26-27 0.6484576643366253 1101 28-29 0.8609529324314451 1101 30-31 1.0383825988152466 1101 32-33 1.3249438078609117 1101 34-35 1.2876740694475046 1101 36-37 1.584812945270066 1101 38-39 1.5515102116935324 1101 40-41 1.9751697520867069 1101 42-43 2.070563360432196 1101 44-45 1.675146317700566 1101 46-47 2.1255614896766737 1101 48-49 1.906949627505714 1101 50-51 2.1393185845547436 1101 52-53 2.282670589982292 1101 54-55 1.9473278203535465 1101 56-57 1.7113174114562426 1101 58-59 1.7532080262785215 1101 60-61 1.435880026353459 1101 62-63 1.523506809113865 1101 64-65 1.1288121669143685 1101 66-67 -0.9969791833638375 1101 68-69 -6.156582966214959 1101 70-71 -9.630512656480917 1101 72-73 -9.329229972140368 1101 74-75 -7.55581393391525 1101 76-77 -2.8949708715982503 1101 78-79 -0.09842329806456007 1101 80-81 0.1991542309171841 1101 82-83 0.12872526373985949 1101 84-85 0.009635706745125816 1101 86-87 0.095822385096195 1101 88-89 0.11368129914278313 1101 90-91 0.15332585127888265 1101 92-93 0.23565786597159644 1101 94-95 -2.692235051589975 1101 96-97 -0.21722710828092318 1101 98-99 0.041346403688582 1101 100-101 0.11333408623461239 1102 1 0.3276662793760181 1102 2 0.08996864350077871 1102 3 0.3775887762527823 1102 4 0.28523962660764113 1102 5 0.3421441028687582 1102 6 0.23334376752650599 1102 7 0.3413616124368204 1102 8 0.42772081953341967 1102 9 0.5037208622150118 1102 10-11 0.5408927784106723 1102 12-13 0.6016693531115251 1102 14-15 0.10977222572044099 1102 16-17 -0.10022263911925222 1102 18-19 0.3826672999465046 1102 20-21 0.5069420507392195 1102 22-23 0.7762188691285061 1102 24-25 0.32154417607966224 1102 26-27 0.42923743093199107 1102 28-29 0.3343712595731745 1102 30-31 0.3200441996257837 1102 32-33 0.3718405019207225 1102 34-35 0.42947582561096453 1102 36-37 0.47690082849269544 1102 38-39 0.5611587913775722 1102 40-41 0.41111950629886707 1102 42-43 0.7159768843918144 1102 44-45 0.5033246733611918 1102 46-47 0.4681400104466853 1102 48-49 0.4833502429134171 1102 50-51 0.6667492573754856 1102 52-53 0.36646829346084786 1102 54-55 0.29981759482913617 1102 56-57 0.6018573941948056 1102 58-59 0.7714967050281913 1102 60-61 0.4316021922883948 1102 62-63 0.22294675057508684 1102 64-65 0.2441588966228032 1102 66-67 -2.3775891499667132 1102 68-69 -7.1992768759301455 1102 70-71 -10.307695665224212 1102 72-73 -8.265207869997699 1102 74-75 -6.925053867871494 1102 76-77 -3.627878297768085 1102 78-79 -1.0343636710616693 1102 80-81 -0.6284800142702487 1102 82-83 -0.33586064942761595 1102 84-85 -0.26039318739724493 1102 86-87 -0.23862262222115405 1102 88-89 -0.1279178227754869 1102 90-91 0.06288624754141026 1102 92-93 -0.5526174914526969 1102 94-95 -3.6695653454096266 1102 96-97 -1.018162415421905 1102 98-99 0.030633056095805244 1102 100-101 0.18350557484581387 1103 1 0.36606208001589735 1103 2 0.16174217974641536 1103 3 0.2712301275416671 1103 4 0.14017801085152826 1103 5 0.26611281447295454 1103 6 0.18055372336753095 1103 7 0.47014036747009413 1103 8 0.27313704521861126 1103 9 0.6271318082875439 1103 10-11 0.6502987611606983 1103 12-13 0.46429906248953046 1103 14-15 0.4884293306922771 1103 16-17 0.22273394397382162 1103 18-19 0.4024384761809543 1103 20-21 0.6817425446384746 1103 22-23 0.9495823716770744 1103 24-25 1.0888960367173581 1103 26-27 0.8815871018685755 1103 28-29 0.6830644337882248 1103 30-31 0.9882314920603648 1103 32-33 1.2556494869942654 1103 34-35 1.4068169810432494 1103 36-37 1.2885287496147981 1103 38-39 1.0960677971316244 1103 40-41 0.8562386017090162 1103 42-43 0.841168483238576 1103 44-45 0.83381899838583 1103 46-47 1.2671117633024203 1103 48-49 0.725737990319228 1103 50-51 0.6152851988861379 1103 52-53 0.7654090255509196 1103 54-55 0.7041215740026487 1103 56-57 0.7660728121235927 1103 58-59 0.6751422532441964 1103 60-61 0.562296935789238 1103 62-63 0.5605302329297395 1103 64-65 0.7360798119178646 1103 66-67 -2.136416382549463 1103 68-69 -7.163575624151015 1103 70-71 -10.927882884668758 1103 72-73 -8.254178771303003 1103 74-75 -6.9394496320163555 1103 76-77 -3.690993390891208 1103 78-79 -1.1799407789531102 1103 80-81 -0.588397839653755 1103 82-83 -0.3138571945658697 1103 84-85 -0.4624965169594297 1103 86-87 -0.2080473999056629 1103 88-89 -0.5611669134412764 1103 90-91 -0.5092659245204381 1103 92-93 -0.05942685364142619 1103 94-95 0.6270494375058355 1103 96-97 0.5615837617249113 1103 98-99 0.256141857477747 1103 100-101 0.13664597118171784 1104 1 0.22859409439593392 1104 2 -0.10741064339322648 1104 3 0.33584924453219855 1104 4 0.14695389844287376 1104 5 0.5471256437217633 1104 6 0.4120088797386856 1104 7 0.4854279682125906 1104 8 0.1670460891341179 1104 9 0.7015373394325408 1104 10-11 0.5375288417546997 1104 12-13 0.5710963909042022 1104 14-15 0.583874771139655 1104 16-17 0.7123380817590146 1104 18-19 0.7964679411589799 1104 20-21 0.8631671551791342 1104 22-23 0.7877922685261609 1104 24-25 0.6199690764974264 1104 26-27 0.4483341471468947 1104 28-29 0.7447229549000909 1104 30-31 0.36596739985895255 1104 32-33 0.575716922789276 1104 34-35 0.33014254085433237 1104 36-37 0.6990603058073788 1104 38-39 0.5560703817788166 1104 40-41 0.4104515766379322 1104 42-43 0.8198642350184571 1104 44-45 0.7795560927724452 1104 46-47 0.4211420506681911 1104 48-49 0.5673211454685507 1104 50-51 0.9207082586384843 1104 52-53 0.6716817880688346 1104 54-55 0.6792502189426131 1104 56-57 1.0694810219029591 1104 58-59 0.9913062843539464 1104 60-61 0.8225899995360351 1104 62-63 0.6983183623500757 1104 64-65 0.5694831461127485 1104 66-67 -2.2373967862243624 1104 68-69 -6.813347117842124 1104 70-71 -7.864912948815 1104 72-73 -7.092839512861779 1104 74-75 -6.406971190323649 1104 76-77 -3.4582605964176523 1104 78-79 -0.915891165475351 1104 80-81 -0.2181647514702867 1104 82-83 -0.03597237584362745 1104 84-85 -0.20780890681821163 1104 86-87 -0.06590208399128628 1104 88-89 -0.06300113182047085 1104 90-91 -0.06177978762812231 1104 92-93 -0.14020735835253006 1104 94-95 0.47765242394818586 1104 96-97 0.7715672552280495 1104 98-99 0.49124342236414265 1104 100-101 0.5278293871454345 1105 1 0.6056965014081648 1105 2 0.5141819084068722 1105 3 0.6704359910501765 1105 4 0.48758112131522324 1105 5 0.4580071058268089 1105 6 0.5463449436197365 1105 7 0.6979316969016978 1105 8 0.651027067083227 1105 9 0.808605213047187 1105 10-11 0.860505024035966 1105 12-13 1.000605523324296 1105 14-15 1.1540271884774853 1105 16-17 1.205989271497664 1105 18-19 1.1035857218432241 1105 20-21 1.2719060551584889 1105 22-23 1.0512643090943001 1105 24-25 1.1626869466702132 1105 26-27 1.2781094506048234 1105 28-29 1.2502672287809204 1105 30-31 0.9880116966855539 1105 32-33 0.8626366813996853 1105 34-35 0.8852253177525213 1105 36-37 1.2391250702689547 1105 38-39 1.2400897939079627 1105 40-41 1.5241536486417928 1105 42-43 1.5870922128069438 1105 44-45 1.0538298588631676 1105 46-47 0.8832786468913163 1105 48-49 0.8342536963508174 1105 50-51 0.8550489116753575 1105 52-53 0.8184845805701535 1105 54-55 1.15412459080191 1105 56-57 1.048594626455401 1105 58-59 1.3199615469683899 1105 60-61 1.1563607367839204 1105 62-63 1.163638685426644 1105 64-65 0.8967258091998715 1105 66-67 1.9061032481943059 1105 68-69 -5.0765265997264315 1105 70-71 -4.622452071368407 1105 72-73 -1.3302092955652505 1105 74-75 0.61087488938999 1105 76-77 0.993802802770066 1105 78-79 1.069422101774137 1105 80-81 1.115162845418265 1105 82-83 1.2782471234794741 1105 84-85 0.991148207520606 1105 86-87 1.180863995263433 1105 88-89 0.9614518981696634 1105 90-91 0.9960048547619103 1105 92-93 1.1433178590587225 1105 94-95 1.7862286957179947 1105 96-97 2.1215922087628485 1105 98-99 1.5470805417510327 1105 100-101 1.529134428333112 1106 1 0.24422691649578354 1106 2 0.34692535988352446 1106 3 0.34600375233723213 1106 4 0.1654026764886538 1106 5 0.26630941471343306 1106 6 0.4300992633913552 1106 7 0.24693641039162628 1106 8 0.322663210146267 1106 9 0.699563325870443 1106 10-11 0.7278817748999202 1106 12-13 0.5266298865579557 1106 14-15 0.8332177147522657 1106 16-17 0.7238095467387637 1106 18-19 0.7382373854770066 1106 20-21 0.6420207482251428 1106 22-23 0.6111720983172688 1106 24-25 0.6566476137931971 1106 26-27 0.9075426075300967 1106 28-29 0.749780298002996 1106 30-31 0.9622474936103771 1106 32-33 1.0396499314817689 1106 34-35 1.0409040436077106 1106 36-37 1.4318147089384894 1106 38-39 1.2849194237849204 1106 40-41 1.1245893821264943 1106 42-43 1.3056337020916828 1106 44-45 1.470694080302593 1106 46-47 1.2880753324230731 1106 48-49 1.3024932885532472 1106 50-51 1.0978005992383117 1106 52-53 1.3534531665788236 1106 54-55 1.6144661657864674 1106 56-57 1.2479955624754773 1106 58-59 1.5059663216724744 1106 60-61 1.1772667619262407 1106 62-63 1.4150104389895901 1106 64-65 1.6554210016627415 1106 66-67 1.9516591005457649 1106 68-69 -4.800393720490831 1106 70-71 -4.7622443327862385 1106 72-73 -1.2122843997684392 1106 74-75 1.4141729411103867 1106 76-77 1.7689785152193558 1106 78-79 1.8680567478728491 1106 80-81 1.8353668555019027 1106 82-83 1.7549300953710443 1106 84-85 1.9004830879025825 1106 86-87 1.7259134396058542 1106 88-89 1.6051910813502381 1106 90-91 1.2528705787305903 1106 92-93 0.9807052389259461 1106 94-95 1.8127483371143462 1106 96-97 2.194435661908976 1106 98-99 2.0338610667458816 1106 100-101 1.7721462760545528 1107 1 0.08305199331594082 1107 2 -0.19927445189292214 1107 3 -0.02362875817947696 1107 4 -0.19451250952383958 1107 5 0.2137825701792906 1107 6 0.05343671647720072 1107 7 -0.030228443961895834 1107 8 -0.038930113586907567 1107 9 -0.07532810542229385 1107 10-11 -0.10067888184154583 1107 12-13 0.010560017067177796 1107 14-15 0.08436859974313649 1107 16-17 -0.1394052208731722 1107 18-19 -0.23947343057415083 1107 20-21 -0.09154087231163288 1107 22-23 0.11518602232484199 1107 24-25 0.27394717271511126 1107 26-27 0.02995028160853508 1107 28-29 0.13618001226603127 1107 30-31 0.09965352356740453 1107 32-33 0.1861241414530781 1107 34-35 0.3354285523555731 1107 36-37 0.24458896539875497 1107 38-39 0.19747484201538867 1107 40-41 0.43555793972176815 1107 42-43 0.457508000505392 1107 44-45 0.05042546487960209 1107 46-47 -0.05479768470980417 1107 48-49 0.14630742346191283 1107 50-51 0.16238622394775604 1107 52-53 0.569657967413356 1107 54-55 0.4403423691132602 1107 56-57 0.6936686884605372 1107 58-59 0.6263698684392267 1107 60-61 0.6289069147728412 1107 62-63 0.7310162444390009 1107 64-65 0.6740024083503435 1107 66-67 1.091539816702376 1107 68-69 -4.427056267404982 1107 70-71 -4.999003486007016 1107 72-73 -1.855430681219346 1107 74-75 0.2480968352969981 1107 76-77 0.5560166021423534 1107 78-79 0.47810516441384365 1107 80-81 0.65161644447155 1107 82-83 0.49391448188698206 1107 84-85 0.4576616621658438 1107 86-87 0.5121549780499457 1107 88-89 -0.03508082231558518 1107 90-91 0.4352660069391767 1107 92-93 0.663222558894649 1107 94-95 1.0762924959478148 1107 96-97 1.0161760494680792 1107 98-99 0.826786809151745 1107 100-101 0.3514127711278583 1108 1 0.1647584779575766 1108 2 -0.20089783815200946 1108 3 0.015750971750779996 1108 4 -0.2644427662384885 1108 5 0.050648574037431615 1108 6 -0.053113216747313174 1108 7 0.20331659239364797 1108 8 -0.03876391682180724 1108 9 0.3617011274015667 1108 10-11 0.03614408892563148 1108 12-13 0.33480842187501736 1108 14-15 0.45204481283114006 1108 16-17 -0.08149306773472631 1108 18-19 0.22781993639424059 1108 20-21 0.20154118734112814 1108 22-23 0.24329256632246654 1108 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100-101 0.3598603415085613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 10.0 4 41.0 5 168.0 6 469.0 7 1000.0 8 1532.0 9 2302.0 10 3739.0 11 5792.0 12 7715.0 13 8629.0 14 7715.0 15 8994.0 16 10314.0 17 11451.0 18 9371.0 19 7063.0 20 6605.0 21 6616.0 22 6964.0 23 7841.0 24 9174.0 25 10933.0 26 13271.0 27 16252.0 28 19311.0 29 22889.0 30 26494.0 31 30589.0 32 35823.0 33 41707.0 34 49273.0 35 54247.0 36 48047.0 37 38215.0 38 16218.0 39 1035.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.22714342575653 18.295484007610032 12.851608002950531 15.625764563682907 2 14.710312555343968 25.015382199736724 41.86861319199359 18.40569205292572 3 20.433067488358255 37.1152199746994 29.038193690182602 13.413518846759754 4 10.409574069983735 18.884911457011793 37.48961780753651 33.21589666546796 5 9.717367057736856 42.06763986980959 32.874721848514 15.340271223939556 6 20.30802481868813 35.770600551648094 26.171887122065375 17.749487507598396 7 23.875665601217936 36.44090227869729 24.504894918521465 15.178537201563309 8 25.200935355677938 30.27712011215506 24.3221683311641 20.199776201002898 9 27.92211940936049 19.29180212955881 25.106925173371202 27.67915328770949 10-11 21.995644499126527 28.385324919269895 29.778501057842732 19.840529523760843 12-13 27.38781299458027 26.78140168269099 30.121775131294804 15.70901019143394 14-15 23.03687572218987 27.040340408131975 33.79310093042699 16.12968293925117 16-17 16.5214367403267 29.86731701440008 35.400353590071 18.21089265520222 18-19 15.866997681677955 29.334623318303795 39.064365382158044 15.734013617860207 20-21 18.275761984472805 27.727801275252688 37.47382774778985 16.52260899248466 22-23 17.235717297691 27.986374912607037 36.34652700830393 18.431380781398033 24-25 17.114142964843843 28.403579323601303 38.04528187538448 16.43699583617037 26-27 18.43531516401672 26.509921323086473 38.97035468501853 16.08440882787828 28-29 16.006109111358 27.38966946713249 40.45423963381848 16.149981787691033 30-31 16.11676308386562 29.858358905645016 39.02295210051972 15.001925909969641 32-33 15.946152434833326 29.621820166731016 37.77928084583177 16.652746552603883 34-35 16.816366992173595 28.39605229287956 37.63548335861808 17.152097356328763 36-37 18.255928154106883 28.356379018148765 37.90711378492826 15.480579042816094 38-39 18.905352636666468 27.610856206248187 37.82961756961461 15.65417358747074 40-41 16.77068522491767 28.861631863854175 39.144483148079786 15.223199763148374 42-43 16.388354659369675 30.658236753265143 36.70484187060022 16.24856671676496 44-45 16.3004521381319 31.327992457360242 35.30423168036602 17.067323724141836 46-47 16.6393040484798 30.034744586632605 34.18280787056705 19.143143494320544 48-49 17.558801602151792 30.464821365922113 34.03074201847737 17.94563501344872 50-51 18.575094764365858 31.138807755100924 34.15509653677241 16.13100094376081 52-53 16.883596114816417 31.464269208075073 34.67208487924108 16.980049797867423 54-55 15.865390762419151 31.411409711021935 35.07747300819572 17.645726518363194 56-57 17.34143259401558 32.419351304366884 32.92709204524289 17.312124056374653 58-59 16.916834403655066 31.446280803129266 33.331872766359496 18.305012026856176 60-61 17.11857924345225 30.521693172887254 34.43715319265853 17.922574391001973 62-63 19.356499309511403 29.88277666778325 33.63697185380444 17.123752168900904 64-65 18.263385603972328 30.736413589149127 34.43363332663977 16.566567480238778 66-67 16.888804980492825 30.918207337850333 34.856117976019505 17.33686970563733 68-69 17.373928435400686 32.380524802154994 33.24154846259575 17.00399829984857 70-71 17.218361931000953 32.230894320921266 32.315517879604336 18.235225868473442 72-73 17.042506047385142 31.824850519988175 31.372818205031756 19.75982522759493 74-75 16.86990142177496 32.23475967844395 32.58501629259955 18.31032260718154 76-77 17.864595599713002 31.0279385909661 33.26048815398631 17.846977655334587 78-79 16.472134619158677 31.038990082471123 34.41432724650513 18.07454805186507 80-81 15.978558102685989 33.40461769312494 33.167489950822 17.44933425336707 82-83 16.00938641470721 33.27093369452715 31.704614530870934 19.01506535989471 84-85 16.408610237435038 31.81313696462114 31.905595522559704 19.872657275384118 86-87 17.22516118668448 31.534358502551967 32.80897461172811 18.431505699035437 88-89 18.14542517216358 30.236716364781422 32.96020956266572 18.65764890038928 90-91 16.414138187234958 31.256514778392567 34.18097712091976 18.148369913452708 92-93 15.802016599017712 33.043509214526736 32.74166740446164 18.41280678199391 94-95 15.305989904921855 33.38583944837478 31.960764942746383 19.347405703956984 96-97 15.68405385304307 32.06071045984761 31.02615167735012 21.229084009759195 98-99 16.484081247392666 31.867172872988647 31.1282199678859 20.520525911732783 100-101 17.175419924531333 31.931424649081652 31.857763286884992 19.035392139502026 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7510.0 1 4986.0 2 5634.0 3 7947.0 4 5872.5 5 4422.0 6 4637.5 7 6218.5 8 7252.0 9 6846.5 10 5727.0 11 4504.0 12 3976.5 13 3922.0 14 3904.5 15 3662.0 16 3393.0 17 3320.5 18 3402.0 19 3445.0 20 3536.5 21 3814.0 22 4127.5 23 4403.5 24 4738.5 25 5099.5 26 5714.0 27 6494.5 28 7116.0 29 7642.0 30 8319.0 31 9198.5 32 10430.0 33 11941.0 34 13323.5 35 14393.0 36 15280.0 37 15906.5 38 15370.5 39 14095.5 40 13173.0 41 12602.0 42 12061.0 43 11996.5 44 12888.0 45 13691.0 46 15223.5 47 20058.0 48 30104.0 49 29564.5 50 19910.0 51 14814.5 52 10481.5 53 8010.0 54 6945.0 55 6174.0 56 5435.0 57 4861.0 58 4288.0 59 3788.0 60 3385.0 61 2866.0 62 2195.0 63 1494.0 64 999.0 65 696.5 66 495.5 67 408.0 68 329.5 69 230.5 70 162.0 71 132.0 72 134.5 73 132.0 74 107.0 75 68.0 76 41.0 77 34.0 78 26.5 79 15.0 80 7.0 81 5.5 82 4.5 83 4.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02099256498111582 2 0.01861949241803316 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.38904151599177E-4 18-19 5.476321299421518E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.476321299421518E-4 28-29 0.01944094061294639 30-31 0.0036508808662810118 32-33 0.008123209927475252 34-35 0.009492290252330633 36-37 0.09683961497810384 38-39 0.10039922382272784 40-41 0.11591546750442214 42-43 0.1014032160609551 44-45 0.09574435071821955 46-47 0.097387247108046 48-49 0.10021667977941377 50-51 0.09756979115136005 52-53 0.07301761732562023 54-55 0.0606046223802648 56-57 0.03486591227298366 58-59 0.014694795486781074 60-61 0.008853386100731454 62-63 0.0022818005414256323 64-65 0.004198512996223164 66-67 0.4097201052183866 68-69 0.7907807956364673 70-71 0.8798622887737239 72-73 0.274272425079361 74-75 0.14338834602318676 76-77 0.013873347291867847 78-79 0.008305753970789302 80-81 0.001277808303198354 82-83 0.0 84-85 0.0 86-87 1.825440433140506E-4 88-89 0.0010039922382272784 90-91 0.0036508808662810118 92-93 0.0017341684114834809 94-95 0.2208782924100012 96-97 0.22845387020753433 98-99 0.014329707400152972 100-101 0.006571585559305821 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 547813.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.43378450800851 #Duplication Level Percentage of deduplicated Percentage of total 1 88.18338308882329 48.88338655332992 2 7.143913238467103 7.920282940101893 3 1.6382045521185649 2.7243563436653737 4 0.7673110254469225 1.7013981614097495 5 0.45709454728080434 1.2669240316874908 6 0.30844776807868446 1.0259056264589992 7 0.22624379718063822 0.8779084929429559 8 0.16023163893436349 0.7105796915242026 9 0.12941387123644668 0.6456510585421511 >10 0.857397850830397 8.852829749077335 >50 0.07031531736663106 2.6190938624851525 >100 0.04444458724374683 4.88186592164862 >500 0.005970168435727187 2.2331705138725573 >1k 0.005306816387313055 5.512323824832302 >5k 0.0019900561452423954 7.794952832713728 >10k+ 3.316760242070659E-4 2.34937039570752 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12778 2.3325477854669385 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9069 1.6554919288151246 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8001 1.460534890555719 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6984 1.2748875985053294 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6556 1.1967587479669157 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5964 1.0886926743249976 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5822 1.0627714201744025 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4628 0.8448138324574263 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4105 0.7493432978041777 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2460 0.44905834655256444 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1885 0.34409552164698537 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1871 0.3415399050405887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1728 0.31543610684667944 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1687 0.3079518010708034 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1531 0.27947493031381143 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1451 0.26487140684868743 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1445 0.2637761425888031 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1429 0.2608554378957783 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1380 0.2519107797733898 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1235 0.2254418934928525 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1101 0.20098099168876973 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1023 0.18674255631027376 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1022 0.18656001226695973 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 954 0.17414701732160429 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 901 0.1644721830259596 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 828 0.1511464678640339 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 794 0.14493997039135617 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 764 0.13946364909193465 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 716 0.13070153501286022 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 714 0.13033644692623214 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 688 0.1255903018000668 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 683 0.12467758158349657 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 656 0.11974889241401719 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 625 0.11409002707128162 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.10331992851575264 No Hit GTACCAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 554 0.10112939999598403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.825440433140506E-4 0.0 12-13 0.0 0.0 0.0 5.476321299421518E-4 0.0 14-15 0.0 0.0 0.0 5.476321299421518E-4 0.0 16-17 0.0 0.0 0.0 5.476321299421518E-4 0.0 18-19 0.0 0.0 0.0 5.476321299421518E-4 0.0 20-21 0.0 0.0 0.0 8.214481949132277E-4 0.0 22-23 0.0 0.0 0.0 0.0022818005414256323 0.0 24-25 0.0 0.0 0.0 0.006480313537648796 0.0 26-27 0.0 0.0 0.0 0.009857378338958733 0.0 28-29 0.0 0.0 0.0 0.012504266967012467 0.0 30-31 0.0 0.0 0.0 0.02172274115437202 0.0 32-33 0.0 0.0 0.0 0.03742152887938037 0.0 34-35 0.0 0.0 0.0 0.06142607057517803 0.0 36-37 0.0 0.0 0.0 0.09903014349787245 0.0 38-39 0.0 0.0 0.0 0.14667413880283967 0.0 40-41 0.0 0.0 0.0 0.20371915233848048 0.0 42-43 0.0 0.0 0.0 0.2616768860906915 0.0 44-45 0.0 0.0 0.0 0.3277578297703778 0.0 46-47 0.0 0.0 0.0 0.4064343124387336 0.0 48-49 0.0 0.0 0.0 0.4902220283198829 0.0 50-51 0.0 0.0 0.0 0.5818591380635363 0.0 52-53 0.0 0.0 0.0 0.667289750334512 0.0 54-55 0.0 0.0 0.0 0.7673238860706117 0.0 56-57 0.0 0.0 0.0 0.8763939519507569 0.0 58-59 0.0 0.0 0.0 0.9968730205380303 0.0 60-61 0.0 0.0 0.0 1.1252014829878079 0.0 62-63 0.0 0.0 0.0 1.2440376551852548 0.0 64-65 0.0 0.0 0.0 1.3634214595126437 0.0 66-67 0.0 0.0 0.0 1.493484090373905 0.0 68-69 0.0 0.0 0.0 1.64061458928503 0.0 70-71 0.0 0.0 0.0 1.8341112751979234 0.0 72-73 0.0 0.0 0.0 2.037282795406462 0.0 74-75 0.0 0.0 0.0 2.2632723210292562 0.0 76-77 0.0 0.0 0.0 2.500397033294208 0.0 78-79 0.0 0.0 0.0 2.763442999709755 0.0 80-81 0.0 0.0 0.0 3.0405448574604836 0.0 82-83 0.0 0.0 0.0 3.333162958892907 0.0 84-85 0.0 0.0 0.0 3.7011717502140327 0.0 86-87 0.0 0.0 0.0 4.088712754169762 0.0 88-89 0.0 0.0 0.0 4.424228705780987 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1410 0.0 68.90427 2 ACCTGGG 1475 0.0 64.24954 3 GCGTATC 30 1.3146612E-4 63.19602 1 GTACCTG 1720 0.0 57.317314 1 CCTGGGA 1095 0.0 54.524094 4 ATAACGC 60 1.5161622E-6 47.384037 3 CCTGGGG 600 0.0 43.435364 4 AGTACTC 305 0.0 40.39295 5 GGAACGC 150 0.0 37.907227 8 AAGTACT 375 0.0 35.38008 4 GTACCAG 230 0.0 35.032578 1 TATCACG 55 0.0026072082 34.467407 2 ATCACGC 85 1.6550524E-5 33.456715 1 TATAACG 85 1.656087E-5 33.453663 2 CTGGGGT 270 0.0 33.344322 5 CTGGGAG 535 0.0 32.77027 5 TCACGCA 90 2.448228E-5 31.595125 2 TCAAACG 75 3.120781E-4 31.589355 3 CATGGGT 520 0.0 30.981869 4 CTGGGAA 595 0.0 30.262074 5 >>END_MODULE