##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939540_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 542331 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.142599261336713 31.0 27.0 31.0 10.0 33.0 2 26.871906640040862 31.0 25.0 31.0 16.0 33.0 3 27.20101377203221 31.0 26.0 31.0 16.0 34.0 4 31.054678784727408 35.0 30.0 37.0 19.0 37.0 5 31.64865736976127 35.0 32.0 37.0 19.0 37.0 6 31.644984336134208 35.0 32.0 37.0 17.0 37.0 7 31.785499999078056 35.0 32.0 37.0 18.0 37.0 8 31.780692971635403 35.0 32.0 37.0 18.0 37.0 9 32.80814299754209 37.0 32.0 39.0 17.0 39.0 10-11 31.77927133060806 35.0 30.0 39.0 14.0 39.0 12-13 31.5051933228969 35.0 30.0 39.0 15.5 39.0 14-15 32.148364190872364 36.0 30.0 39.0 16.5 41.0 16-17 32.23746383666064 36.0 29.5 39.0 17.0 40.0 18-19 32.506815948194 36.0 30.0 39.0 17.5 40.0 20-21 32.49753379393765 36.0 30.5 39.0 17.5 40.0 22-23 32.42208909319216 36.0 30.0 39.0 17.0 40.0 24-25 32.22834210104162 36.0 30.0 39.0 16.0 41.0 26-27 32.07518010218851 36.0 30.0 39.0 16.0 41.0 28-29 31.516791405986382 35.0 29.5 39.0 12.0 40.5 30-31 31.015706275318948 35.0 28.5 38.5 10.0 40.0 32-33 30.611834838871467 35.0 27.5 38.0 9.0 40.0 34-35 29.920719081151546 35.0 25.5 38.0 8.0 40.0 36-37 29.335073967742947 34.5 24.5 38.0 6.5 40.0 38-39 28.820705436347914 34.0 24.0 38.0 2.0 40.0 40-41 28.573641373994846 33.5 23.5 38.0 2.0 40.0 42-43 28.257819486623482 33.0 23.0 38.0 2.0 40.0 44-45 27.774184953469376 33.0 22.0 38.0 2.0 40.0 46-47 26.946520667267777 33.0 19.5 38.0 2.0 40.0 48-49 26.47430074991103 33.0 16.5 38.0 2.0 40.0 50-51 25.776993201568782 32.0 15.0 37.0 2.0 39.5 52-53 25.785897357886604 32.5 14.0 37.0 2.0 39.0 54-55 25.939800601477696 33.0 13.0 37.0 2.0 40.0 56-57 25.59069092491486 33.0 10.0 37.0 2.0 40.0 58-59 25.263843483038954 32.0 8.0 37.0 2.0 39.5 60-61 24.654735761002044 31.0 7.5 36.5 2.0 39.0 62-63 24.023676500144745 31.0 4.0 36.0 2.0 39.0 64-65 23.442811677739243 30.0 2.0 35.0 2.0 39.0 66-67 23.117277087240083 29.5 2.0 35.0 2.0 38.0 68-69 22.521286815616293 29.0 2.0 35.0 2.0 38.0 70-71 21.90043257715307 28.5 2.0 34.5 2.0 37.5 72-73 21.398130477512808 28.0 2.0 34.0 2.0 36.5 74-75 20.681342206143484 26.5 2.0 34.0 2.0 36.0 76-77 19.889234618710713 26.0 2.0 33.0 2.0 35.5 78-79 19.39228349476611 25.5 2.0 33.0 2.0 35.0 80-81 18.99824922418228 25.0 2.0 33.0 2.0 35.0 82-83 18.367638951120256 24.0 2.0 32.5 2.0 35.0 84-85 17.88566576500329 22.5 2.0 32.0 2.0 35.0 86-87 17.15295271706762 20.0 2.0 32.0 2.0 34.5 88-89 16.33290923808523 15.5 2.0 31.0 2.0 34.0 90-91 15.561966769371473 6.5 2.0 31.0 2.0 34.0 92-93 14.818878138996295 2.0 2.0 31.0 2.0 34.0 94-95 13.871146956379038 2.0 2.0 30.0 2.0 34.0 96-97 12.828531837567834 2.0 2.0 29.0 2.0 34.0 98-99 11.918529458946658 2.0 2.0 29.0 2.0 33.5 100-101 10.748618463631988 2.0 2.0 26.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.667693991246189 1101 2 3.671879755925332 1101 3 3.291871126738002 1101 4 2.8385690024265458 1101 5 2.0550533881520217 1101 6 2.579482513386967 1101 7 1.9885659275537115 1101 8 2.3605966778306886 1101 9 2.2616123864486397 1101 10-11 3.0857846358651813 1101 12-13 3.406326926646912 1101 14-15 2.7910980136059145 1101 16-17 2.2630822391697123 1101 18-19 2.6156407606427123 1101 20-21 2.4664779452376706 1101 22-23 2.6241486358258825 1101 24-25 3.0788060212367796 1101 26-27 2.729999286756506 1101 28-29 2.9471481792427774 1101 30-31 3.30275496010276 1101 32-33 3.0716452427765653 1101 34-35 3.4896901540757845 1101 36-37 3.2624413788395223 1101 38-39 3.6637619211332932 1101 40-41 3.482251248574414 1101 42-43 3.3860564192602496 1101 44-45 3.6983848257002236 1101 46-47 3.2241186818836987 1101 48-49 3.514212330058367 1101 50-51 3.231749881650874 1101 52-53 2.872258801575949 1101 54-55 2.957676514089936 1101 56-57 3.0076813586327873 1101 58-59 2.8424220897227563 1101 60-61 2.892829944838411 1101 62-63 2.9859242177756578 1101 64-65 3.1711974695415357 1101 66-67 3.1782465439522802 1101 68-69 3.428414046402718 1101 70-71 2.8763334404304395 1101 72-73 3.16869208796248 1101 74-75 3.2449976956993787 1101 76-77 3.065952763464903 1101 78-79 2.5348363898176878 1101 80-81 2.7928684710944474 1101 82-83 1.998974265492432 1101 84-85 1.9118670295809146 1101 86-87 2.0309373954416508 1101 88-89 2.0321867664221607 1101 90-91 1.8222566221472594 1101 92-93 1.1317076883694828 1101 94-95 1.7104551088540614 1101 96-97 1.1337564424984592 1101 98-99 1.00437903808524 1101 100-101 0.9512871831359302 1102 1 3.6239747280970676 1102 2 2.47826083837543 1102 3 2.976609619053388 1102 4 2.8552462486611816 1102 5 2.0343398764407645 1102 6 2.4719908968931 1102 7 2.63860664286074 1102 8 2.734404873469341 1102 9 2.390535642903778 1102 10-11 1.617722107412547 1102 12-13 3.1903450465615038 1102 14-15 3.1269207513416326 1102 16-17 2.9189721731916762 1102 18-19 2.9645068233122274 1102 20-21 2.9507464482608725 1102 22-23 2.9365584690534234 1102 24-25 3.0087660753528738 1102 26-27 2.6465649666380138 1102 28-29 3.025273479798688 1102 30-31 3.4250579717531124 1102 32-33 2.6557191458677316 1102 34-35 3.518767936341863 1102 36-37 3.34752354681741 1102 38-39 3.606903155159749 1102 40-41 3.766038541551506 1102 42-43 3.541707511520496 1102 44-45 2.9635456671926512 1102 46-47 3.6462514586676384 1102 48-49 3.4724054039678727 1102 50-51 3.56024707005054 1102 52-53 3.958065509663225 1102 54-55 3.9697340156448107 1102 56-57 3.897746823717007 1102 58-59 4.009814899463652 1102 60-61 4.03344682982889 1102 62-63 4.00855647840492 1102 64-65 3.9038309484240763 1102 66-67 3.651177276635451 1102 68-69 3.540563878732595 1102 70-71 3.228557714447664 1102 72-73 3.5079195230263736 1102 74-75 3.5343681152273376 1102 76-77 3.1575189242358697 1102 78-79 3.0827025230176552 1102 80-81 3.1933756491710206 1102 82-83 2.97532091625769 1102 84-85 2.6710359884873114 1102 86-87 2.3170217370398696 1102 88-89 2.0764863028447316 1102 90-91 2.026652272341858 1102 92-93 2.061270207204318 1102 94-95 1.7042596497328706 1102 96-97 1.836178842970373 1102 98-99 1.8839098182161713 1102 100-101 1.2704791606174801 1103 1 3.7286258908877663 1103 2 3.7305715121977343 1103 3 3.341938331039078 1103 4 3.0083718698100554 1103 5 2.533492008223707 1103 6 2.654863337759725 1103 7 2.4637563397400903 1103 8 2.4079197065045186 1103 9 2.5241571893160213 1103 10-11 3.4336228975139207 1103 12-13 2.5179420700160904 1103 14-15 2.8918764634267085 1103 16-17 2.471527579671502 1103 18-19 2.5762142373452193 1103 20-21 2.7871544685351637 1103 22-23 2.8879815211305484 1103 24-25 2.9629346054661667 1103 26-27 2.573721284964389 1103 28-29 2.179087023328794 1103 30-31 2.8671870837586795 1103 32-33 2.764269570733557 1103 34-35 2.87437877414747 1103 36-37 2.8945538340366532 1103 38-39 3.693947853032938 1103 40-41 3.5854938561012943 1103 42-43 3.672794770514731 1103 44-45 3.5361424421876784 1103 46-47 3.412486737439256 1103 48-49 3.3177517565816643 1103 50-51 3.9389351415075033 1103 52-53 3.6307433445867012 1103 54-55 3.4573684943766736 1103 56-57 3.785179118489417 1103 58-59 3.636356901550709 1103 60-61 3.4889880989602737 1103 62-63 4.047080131754154 1103 64-65 3.745430220437594 1103 66-67 3.3911441694003095 1103 68-69 3.233017541026374 1103 70-71 3.3842859493910176 1103 72-73 2.7039463442348826 1103 74-75 2.7131316562728536 1103 76-77 2.507513023321536 1103 78-79 2.5420048485990527 1103 80-81 2.459367127008427 1103 82-83 2.507654821047989 1103 84-85 2.361099780476973 1103 86-87 2.673438515513336 1103 88-89 2.393717904414995 1103 90-91 1.658418136484178 1103 92-93 1.696391828154427 1103 94-95 1.6734087378863212 1103 96-97 1.5318019174088526 1103 98-99 1.3270156868307623 1103 100-101 1.1443595397667536 1104 1 3.534086641741009 1104 2 3.6266181561112703 1104 3 2.555568877114162 1104 4 2.3778731970758855 1104 5 1.5700569968471463 1104 6 1.8414982448511203 1104 7 2.037093702273271 1104 8 2.2357602923975826 1104 9 2.409917523028575 1104 10-11 2.8057254287994766 1104 12-13 3.100783603956174 1104 14-15 3.223346291830193 1104 16-17 3.0463403403844396 1104 18-19 2.658220304846168 1104 20-21 2.127349292198417 1104 22-23 2.2800167410774534 1104 24-25 2.4520735417574073 1104 26-27 2.4152996647520233 1104 28-29 2.1166314630330056 1104 30-31 1.8151896671868144 1104 32-33 1.9921652618598351 1104 34-35 2.4506550824523607 1104 36-37 2.3276394900851933 1104 38-39 2.7346605473825747 1104 40-41 2.4862422207201824 1104 42-43 2.214129620723302 1104 44-45 1.8089607489756965 1104 46-47 2.229617354617865 1104 48-49 1.912911976528573 1104 50-51 2.1091184701006043 1104 52-53 2.2767751752655023 1104 54-55 2.3121543542553233 1104 56-57 2.0286790710870655 1104 58-59 2.0830914720854103 1104 60-61 2.065074797860543 1104 62-63 1.920634360043877 1104 64-65 2.3404452706840857 1104 66-67 2.0013042708413415 1104 68-69 2.0740952334799196 1104 70-71 1.714241865204439 1104 72-73 1.7661115651298367 1104 74-75 1.5047686964700482 1104 76-77 1.6456345866510773 1104 78-79 1.7322778861417127 1104 80-81 1.6320124152147208 1104 82-83 1.9533732036797673 1104 84-85 1.3461887783912694 1104 86-87 1.5194904921913803 1104 88-89 1.6198597085484785 1104 90-91 1.5188448761504656 1104 92-93 1.4064731231866627 1104 94-95 1.806920422565879 1104 96-97 1.6462400810417677 1104 98-99 1.2081602165994383 1104 100-101 1.0365991823530276 1105 1 3.435756620938701 1105 2 3.2844056944275017 1105 3 2.9707053089677693 1105 4 2.6110647579443693 1105 5 0.5356230919814884 1105 6 2.2030441055393872 1105 7 2.4479132205173038 1105 8 2.209021503335311 1105 9 2.645502218555791 1105 10-11 2.570410178215681 1105 12-13 3.267532359584095 1105 14-15 3.196823348448401 1105 16-17 2.985351622304968 1105 18-19 3.074044835345596 1105 20-21 3.1937935460677096 1105 22-23 3.1585289414286066 1105 24-25 2.31272414989175 1105 26-27 2.8796880247304735 1105 28-29 2.8806512918630354 1105 30-31 2.8102063843623384 1105 32-33 2.954356052057829 1105 34-35 3.0234573678090406 1105 36-37 2.41590298985631 1105 38-39 2.667487633263079 1105 40-41 2.9954091433112566 1105 42-43 2.9102971285603836 1105 44-45 2.625332571408581 1105 46-47 2.6598383574166142 1105 48-49 2.879284957864442 1105 50-51 2.824326753939122 1105 52-53 2.5716551418890994 1105 54-55 2.599347896847906 1105 56-57 2.8615768500495022 1105 58-59 2.8708601202830266 1105 60-61 3.044357958420754 1105 62-63 3.102553155711899 1105 64-65 3.0689997709940933 1105 66-67 3.038037149498404 1105 68-69 3.128659283150501 1105 70-71 2.9624021067198214 1105 72-73 2.9445362683058107 1105 74-75 3.2059484597061676 1105 76-77 3.328686209984429 1105 78-79 3.127318940279867 1105 80-81 3.3376713384779606 1105 82-83 3.2444939787540825 1105 84-85 3.1396070786282664 1105 86-87 3.2712694082694185 1105 88-89 3.5460520748545328 1105 90-91 3.020002330504175 1105 92-93 2.9608908613557094 1105 94-95 3.31001179627153 1105 96-97 3.7140243164844975 1105 98-99 3.2452769818694325 1105 100-101 2.9721323066975245 1106 1 3.1535822608703157 1106 2 2.538381285321641 1106 3 2.291054823185675 1106 4 1.5712718116366986 1106 5 1.6105807953092217 1106 6 1.873867846194866 1106 7 2.040208855737763 1106 8 0.5881389473422196 1106 9 1.7939361305609296 1106 10-11 0.39956421514045104 1106 12-13 1.420274458032381 1106 14-15 1.603268442775164 1106 16-17 2.114979441218914 1106 18-19 2.253061241417356 1106 20-21 1.8924664231599522 1106 22-23 2.338906841084004 1106 24-25 2.6341551552043896 1106 26-27 2.5797431363314693 1106 28-29 1.8024372632938999 1106 30-31 2.1343618583369164 1106 32-33 2.477334034084347 1106 34-35 2.8357934774633513 1106 36-37 1.8766018997725453 1106 38-39 2.6423644469829064 1106 40-41 2.044304392750508 1106 42-43 2.0677918618468993 1106 44-45 1.9695430384645825 1106 46-47 2.1413670821163926 1106 48-49 1.74187188456305 1106 50-51 2.1889033279531134 1106 52-53 1.9882958823993846 1106 54-55 1.6637884696529959 1106 56-57 2.0848173529280416 1106 58-59 2.281494881480903 1106 60-61 2.0866738901179254 1106 62-63 2.8354381887640407 1106 64-65 3.1155809257895406 1106 66-67 2.3693882429896043 1106 68-69 2.1070813135685498 1106 70-71 2.3595804520088173 1106 72-73 2.5001441700056795 1106 74-75 2.3599557545858296 1106 76-77 2.448644313315718 1106 78-79 2.6087587753006254 1106 80-81 1.9608487296844004 1106 82-83 2.0996141724150625 1106 84-85 1.7349590373123434 1106 86-87 2.256621870661675 1106 88-89 2.6167445550839865 1106 90-91 2.3953064072811525 1106 92-93 1.704799696100908 1106 94-95 2.0168269679619453 1106 96-97 2.0714734197357867 1106 98-99 1.8130449916591491 1106 100-101 1.4720982323205654 1107 1 2.7756951626462047 1107 2 3.337661506277069 1107 3 0.03404534702486828 1107 4 1.0599063083338365 1107 5 1.8119019401360852 1107 6 2.173729284079048 1107 7 1.8249137060317828 1107 8 1.6017936703884779 1107 9 1.420298890519888 1107 10-11 1.31893780278331 1107 12-13 2.02503144735968 1107 14-15 2.289543043750367 1107 16-17 0.9393031372006178 1107 18-19 0.4310593133270615 1107 20-21 -0.7848000903754269 1107 22-23 0.45751539225744153 1107 24-25 1.2310943893619637 1107 26-27 0.9347969337703894 1107 28-29 1.676917655359592 1107 30-31 2.478308987745251 1107 32-33 1.0236639979093987 1107 34-35 0.8738329630166213 1107 36-37 1.0223075591205308 1107 38-39 1.742056324515076 1107 40-41 2.0172165840468246 1107 42-43 1.5086937245309109 1107 44-45 0.9739168895924557 1107 46-47 2.321562928972707 1107 48-49 2.1965385140318325 1107 50-51 2.4593691384484977 1107 52-53 1.9074803808014238 1107 54-55 2.2928465262495763 1107 56-57 3.0720383300544434 1107 58-59 3.045553356553672 1107 60-61 2.7170292228994875 1107 62-63 2.2314964530488055 1107 64-65 1.563223354935321 1107 66-67 1.8600252257452858 1107 68-69 2.2542733383423403 1107 70-71 1.0876422252939157 1107 72-73 1.9469170986260416 1107 74-75 2.0874316612067396 1107 76-77 2.0969073271502303 1107 78-79 2.1063920599667263 1107 80-81 2.223450287655755 1107 82-83 2.2083683843004067 1107 84-85 2.4152013815229587 1107 86-87 2.7806811516885865 1107 88-89 2.526533488093701 1107 90-91 2.3396382248007352 1107 92-93 2.3575109568301738 1107 94-95 1.8191295169469086 1107 96-97 1.5480914501698564 1107 98-99 1.660308932340925 1107 100-101 1.2526043715212438 1108 1 2.615385472769301 1108 2 2.6322846364718337 1108 3 1.8446604564746174 1108 4 2.0496513897481137 1108 5 1.8856080070622703 1108 6 -0.09543912062200022 1108 7 0.26202264829603195 1108 8 -0.5534442598137126 1108 9 1.4081099574151352 1108 10-11 2.7698416838089273 1108 12-13 1.797020418835288 1108 14-15 2.3374369792997207 1108 16-17 0.2356947302715824 1108 18-19 0.8835119531795215 1108 20-21 1.5575885568046246 1108 22-23 -0.21349326593875162 1108 24-25 1.4325738331084423 1108 26-27 1.8410854206593221 1108 28-29 2.1191911899954654 1108 30-31 1.539476451741642 1108 32-33 1.4949152588868664 1108 34-35 1.9959126010928792 1108 36-37 1.0045579474706408 1108 38-39 2.2865061670089304 1108 40-41 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-0.5752756331460169 2312 42-43 -2.8738002115641237 2312 44-45 -2.7519735972388446 2312 46-47 -0.8273150098725743 2312 48-49 0.247366451922165 2312 50-51 -1.0200422330444674 2312 52-53 -1.2574903023667012 2312 54-55 -0.6613199016806739 2312 56-57 -0.22314301412707138 2312 58-59 -0.5473628565138036 2312 60-61 -0.05071844225836131 2312 62-63 -0.6540399399304349 2312 64-65 -2.1931394480211885 2312 66-67 0.78093471599529 2312 68-69 0.2739337596643665 2312 70-71 -2.1249210398562965 2312 72-73 -3.0881179478798124 2312 74-75 -0.747475422580191 2312 76-77 -0.8012515376103657 2312 78-79 -0.7692854739815935 2312 80-81 -2.401038338941394 2312 82-83 -0.7555060212459175 2312 84-85 0.8754871012655023 2312 86-87 1.1918692950832295 2312 88-89 1.7074555836264658 2312 90-91 2.1858766938318457 2312 92-93 2.3281234589787996 2312 94-95 2.2536450730117696 2312 96-97 1.649168628878387 2312 98-99 0.6599345936407968 2312 100-101 1.203258509300806 2313 1 -8.52807514004007 2313 2 -4.443569381273058 2313 3 -1.8692085761774173 2313 4 0.5178506785156678 2313 5 0.931870444649828 2313 6 -3.914516673695026 2313 7 -1.0592927725164962 2313 8 -2.278560104492044 2313 9 0.6554859407478659 2313 10-11 -1.5116842330702696 2313 12-13 -2.4852759998579117 2313 14-15 -3.222135922025757 2313 16-17 -3.796506498220822 2313 18-19 -1.2311168050373666 2313 20-21 -3.165573194695071 2313 22-23 -1.8768989198774726 2313 24-25 -3.1714618721970567 2313 26-27 -2.151022772882115 2313 28-29 -2.5498809749529876 2313 30-31 -4.851511011888519 2313 32-33 -6.958877980812833 2313 34-35 -5.216654301453907 2313 36-37 -2.466317443168389 2313 38-39 -2.028535680758587 2313 40-41 -4.206425173108325 2313 42-43 -3.581271667056864 2313 44-45 -3.810725398591245 2313 46-47 -1.9881951783683078 2313 48-49 -2.844519148343881 2313 50-51 -3.1878063390194136 2313 52-53 -0.734987253924178 2313 54-55 -2.073314137352991 2313 56-57 -2.761187576814134 2313 58-59 -1.788090048621111 2313 60-61 -1.5497540258965437 2313 62-63 -3.598724564694873 2313 64-65 -4.792873402128272 2313 66-67 -3.93388625728927 2313 68-69 -3.065396691505125 2313 70-71 -2.4859674870351007 2313 72-73 -2.103171711320673 2313 74-75 -3.0443604685839603 2313 76-77 -3.201140685154982 2313 78-79 -3.661603398823628 2313 80-81 -4.120526200597528 2313 82-83 -1.692320121678243 2313 84-85 -1.7858808180339079 2313 86-87 -2.869565135689413 2313 88-89 -3.588908455837011 2313 90-91 -2.986554300514678 2313 92-93 -1.309854592235034 2313 94-95 -0.6218787048399115 2313 96-97 -0.5533588071043205 2313 98-99 -0.3327602295495371 2313 100-101 -0.30390255931686383 2314 1 -7.046265884004008 2314 2 -3.864125457499238 2314 3 -24.939311668960922 2314 4 -13.180841694403508 2314 5 -6.7662239008672 2314 6 -5.471534569888181 2314 7 -4.541271618412868 2314 8 -3.671963951504136 2314 9 -3.2395513243924903 2314 10-11 -14.406406864390839 2314 12-13 -12.894643608194592 2314 14-15 -4.858450466587723 2314 16-17 -3.0847043828104574 2314 18-19 -3.016498605367623 2314 20-21 -4.2657405531098505 2314 22-23 -4.243083594309567 2314 24-25 -5.105991260334697 2314 26-27 -3.7712575209519166 2314 28-29 -3.498774177969903 2314 30-31 -3.922935120426029 2314 32-33 -3.9158914141149275 2314 34-35 -3.561024364380671 2314 36-37 -4.4244622193544 2314 38-39 -1.3248337126236294 2314 40-41 -2.560305656885717 2314 42-43 -4.780307682587722 2314 44-45 -6.037743741073502 2314 46-47 -5.315563857798839 2314 48-49 -3.5614870601571518 2314 50-51 -3.2714386860538234 2314 52-53 -1.074129039973183 2314 54-55 -3.554344618898938 2314 56-57 -3.285550499115203 2314 58-59 -1.3891098192285085 2314 60-61 -4.101898445051269 2314 62-63 -5.62238235020833 2314 64-65 -4.578895627325753 2314 66-67 -5.502175184856547 2314 68-69 -4.3343751357413005 2314 70-71 -5.192418596063529 2314 72-73 -4.70966521781418 2314 74-75 -3.773994065227864 2314 76-77 -3.38492700193099 2314 78-79 -4.9168696102754055 2314 80-81 -6.691584352068052 2314 82-83 -6.427793411275243 2314 84-85 -4.618529097589404 2314 86-87 -4.240556343794024 2314 88-89 -3.658917827908237 2314 90-91 -5.455206953703415 2314 92-93 -5.441978482090883 2314 94-95 -5.521204808289726 2314 96-97 -5.141775373355937 2314 98-99 -5.632287163097089 2314 100-101 -4.748103032383317 2315 1 -24.771374109112234 2315 2 -24.561095154468934 2315 3 -13.437236980164242 2315 4 -28.838850352412166 2315 5 -15.849525799521796 2315 6 -12.354804135730362 2315 7 -9.52249164320136 2315 8 -8.821700798336419 2315 9 -6.224229163842114 2315 10-11 -29.561168769152744 2315 12-13 -29.327544041095024 2315 14-15 -23.90515341785499 2315 16-17 -14.593407298152378 2315 18-19 -9.609931451912502 2315 20-21 -7.229918491354184 2315 22-23 -4.180663303673512 2315 24-25 -5.429332856497865 2315 26-27 -6.374515949936484 2315 28-29 -17.801970635730676 2315 30-31 -17.010359452663636 2315 32-33 -9.364652887772667 2315 34-35 -7.107489310223212 2315 36-37 -4.982643319029279 2315 38-39 -5.267591162642489 2315 40-41 -9.154510898393863 2315 42-43 -8.52407014419251 2315 44-45 -5.055957892067738 2315 46-47 -4.346190216226031 2315 48-49 -4.914987985234841 2315 50-51 -3.5366620518995937 2315 52-53 -7.5777664813699595 2315 54-55 -7.293881361547715 2315 56-57 -8.105474696632761 2315 58-59 -7.937694360551639 2315 60-61 -5.4017089388451645 2315 62-63 -4.088783169306243 2315 64-65 -4.605152565408421 2315 66-67 -6.592478035532842 2315 68-69 -6.900576569162652 2315 70-71 -7.882787010498333 2315 72-73 -6.590101171901587 2315 74-75 -7.16602492510105 2315 76-77 -6.954994900837063 2315 78-79 -6.894717143102191 2315 80-81 -7.920092013148327 2315 82-83 -7.1599699484310335 2315 84-85 -6.6794899209983605 2315 86-87 -6.990861709244619 2315 88-89 -6.130720921080627 2315 90-91 -6.081363300814118 2315 92-93 -5.706537229014776 2315 94-95 -6.070233925783574 2315 96-97 -6.272287668805992 2315 98-99 -6.311981258766289 2315 100-101 -5.9098164918146185 2316 1 -1.2816246785883152 2316 2 2.1086087179684228 2316 3 2.1768614517680085 2316 4 2.4549993059021844 2316 5 -0.6194716402197216 2316 6 -1.7611784870947425 2316 7 -2.734346197592231 2316 8 -3.6965672904582725 2316 9 -2.8297405580896964 2316 10-11 -3.045223438856617 2316 12-13 -4.271735385058577 2316 14-15 -1.8826248495320392 2316 16-17 -2.108112068518828 2316 18-19 -3.680303100037719 2316 20-21 -1.7032830789211744 2316 22-23 -2.13283203872772 2316 24-25 -2.930015755202014 2316 26-27 -3.3756170945597823 2316 28-29 -2.121127716610456 2316 30-31 0.9639881309619156 2316 32-33 -1.3866237948700864 2316 34-35 -8.253551654857848 2316 36-37 -2.895305537008653 2316 38-39 -0.8002426999916139 2316 40-41 -1.1596952574142705 2316 42-43 -1.516144738470338 2316 44-45 -0.7805772173921746 2316 46-47 0.43552085854783584 2316 48-49 0.9812704860172516 2316 50-51 1.45507227451181 2316 52-53 1.037525251066075 2316 54-55 -1.3090138297184915 2316 56-57 0.0474426277275839 2316 58-59 -3.434655579466753 2316 60-61 -3.9522174502677707 2316 62-63 -5.434396076800642 2316 64-65 -5.219489657441425 2316 66-67 -2.511560446519713 2316 68-69 -2.1930415262983516 2316 70-71 -2.2599101402065536 2316 72-73 -1.976520230682862 2316 74-75 -1.7304229817934562 2316 76-77 -1.5014107459773776 2316 78-79 -2.1920498211048205 2316 80-81 -2.781477754034345 2316 82-83 -3.5865736970486957 2316 84-85 -2.6637099986928554 2316 86-87 -1.9966628037729208 2316 88-89 -3.3945773847523064 2316 90-91 -3.461577196975707 2316 92-93 -3.4000196815899404 2316 94-95 -4.70952635765911 2316 96-97 -4.24856816295055 2316 98-99 -4.6397371921872095 2316 100-101 -4.455249340896367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7485.0 3 2521.0 4 2080.0 5 2290.0 6 3286.0 7 4510.0 8 5818.0 9 7423.0 10 9024.0 11 10988.0 12 12914.0 13 13437.0 14 13124.0 15 14064.0 16 14427.0 17 13932.0 18 12173.0 19 11695.0 20 12013.0 21 12848.0 22 14119.0 23 15322.0 24 16771.0 25 18055.0 26 19501.0 27 20658.0 28 22930.0 29 24061.0 30 25730.0 31 27160.0 32 28613.0 33 30201.0 34 30387.0 35 26785.0 36 21014.0 37 12506.0 38 2444.0 39 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.949915379324736 16.727459602321463 12.186729623418675 15.135895394935126 2 14.66038597109289 23.558290574979118 43.93986745383708 17.841456000090915 3 20.498656231899172 38.16264157057902 27.875691672735197 13.46301052478661 4 10.100690325572215 17.847346241012453 37.7930438571825 34.25891957623284 5 9.717842860177514 41.06711737921735 32.793401863807226 16.421637896797908 6 20.024952752185214 35.201652197023385 27.01947495865816 17.75392009213324 7 24.812762768859375 36.041828926629584 24.607161708740442 14.538246595770598 8 25.38544029620715 28.83589924379318 24.56407823796612 21.214582222033545 9 29.73293220870785 18.731474132998652 24.969085103634114 26.566508554659386 10-11 22.296692258759833 27.807088674676606 29.859874598430274 20.036344468133287 12-13 28.404160658998684 26.173097561409204 30.016102577441607 15.406639202150505 14-15 23.901187276754055 26.285845662365446 33.96564527059286 15.847321790287635 16-17 16.87241507226263 29.352229335803916 35.124842435908555 18.6505131560249 18-19 16.17544866689615 28.72169501817554 39.409781662773774 15.693074652154534 20-21 19.02478334877985 26.580806197314153 37.95790301557821 16.436507438327787 22-23 17.749774262822704 27.223626603934196 36.79405198694879 18.23254714629431 24-25 17.59605529239037 27.622925842821132 38.51251650553571 16.268502359252796 26-27 18.896554525947238 25.639401538626316 39.528680275224275 15.93536366020217 28-29 16.428101722991368 26.740915039469183 40.65552879242206 16.17545444511739 30-31 16.35936229956403 29.18911180078327 39.4050653956994 15.046460503953298 32-33 16.305171928172022 28.789830680648095 38.240039155388715 16.664958235791165 34-35 17.329265838025297 27.751422361498644 37.837955123363656 17.0813566771124 36-37 18.738459777246007 27.541341693993914 38.33403497887677 15.386163549883305 38-39 19.343403334379783 26.86117678435992 38.29443408798147 15.500985793278826 40-41 17.042886681682237 28.377210796440572 39.36408448103977 15.215818040837426 42-43 16.576880117417915 29.945537288495444 37.08587569578422 16.391706898302424 44-45 16.676990680692555 30.479997415149988 35.628649369712015 17.214362534445435 46-47 17.176677616575912 29.12121900372445 34.51012543462645 19.191977945073187 48-49 18.093517075296777 29.785367619357174 34.29852036255919 17.82259494278686 50-51 19.007388252678243 30.535833025489474 34.438954562246025 16.017824159586258 52-53 16.938532892287768 31.169457379091963 34.96659366198679 16.925416066633474 54-55 15.941102630725762 30.880938899659995 35.264157897118956 17.913800572495287 56-57 17.629910484256715 31.975607098467318 33.084627190106914 17.309855227169052 58-59 17.08305174253923 30.897988426156736 33.574762817458996 18.444197013845034 60-61 17.445863870479425 29.974985692923994 34.69096715833779 17.888183278258783 62-63 19.580745570957 29.472059551150924 33.82540819566807 17.12178668222401 64-65 18.38963330942696 30.324545201971954 34.5984328863118 16.68738860228929 66-67 16.947780563175403 30.611909605437553 34.77434587254529 17.66596395884175 68-69 16.680511329549123 32.45761225714154 33.67077391665513 17.191102496654207 70-71 16.859782440126725 32.24174919319701 32.27738145350082 18.621086913175443 72-73 17.12291774185954 31.483586132527776 31.570555579662447 19.822940545950242 74-75 17.344590826325103 31.897707504625988 32.2875462137077 18.470155455341214 76-77 18.536955234797738 30.277257770024097 33.171651198808995 18.01413579636917 78-79 17.045181668729334 30.630807026617656 34.04538485693703 18.27862644771598 80-81 16.4378526299068 32.91911296809903 33.03973098303682 17.603303418957346 82-83 16.642209307587382 32.4212231388473 31.602632713880297 19.333934839685014 84-85 16.930830479317656 31.338708024270133 31.94232195143572 19.788139544976485 86-87 17.910005373971146 30.877896358072682 32.84982982424714 18.362268443709038 88-89 18.73372630272678 29.67888417374861 32.91146370019094 18.675925823333667 90-91 17.01495018145217 30.719806451017146 33.98511445799821 18.280128909532472 92-93 16.465500048014064 32.303216203638726 32.61964735516373 18.61163639318348 94-95 16.12484513995837 32.51640523304531 31.766148172305066 19.592601454691255 96-97 16.833743056190695 31.202220171298332 30.7046401690996 21.25939660341137 98-99 17.489441008710532 31.25858740978311 30.731353944040812 20.520617637465545 100-101 18.20183201337549 31.352456933082806 31.503753769712723 18.941957283828984 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4510.0 1 3557.0 2 5443.0 3 7383.0 4 5381.0 5 4052.5 6 4177.5 7 5423.0 8 6663.0 9 6654.0 10 5595.5 11 4547.0 12 4123.0 13 3971.0 14 3829.5 15 3734.0 16 3570.5 17 3448.0 18 3501.5 19 3600.0 20 3698.5 21 3788.5 22 4045.0 23 4437.0 24 4787.0 25 5214.5 26 5862.0 27 6540.5 28 7199.5 29 7860.5 30 8627.0 31 9468.5 32 10392.0 33 11689.5 34 13096.5 35 14191.5 36 15104.5 37 15467.5 38 15024.5 39 14368.5 40 13560.0 41 13017.5 42 12793.5 43 12859.5 44 13568.5 45 14442.0 46 15963.5 47 19389.5 48 25664.0 49 25914.5 50 20013.5 51 15622.0 52 11664.5 53 8970.5 54 7462.5 55 6463.0 56 5608.0 57 4859.5 58 4259.0 59 3752.0 60 3222.5 61 2773.0 62 2255.5 63 1640.0 64 1167.0 65 847.5 66 626.5 67 470.5 68 368.0 69 280.5 70 231.5 71 186.0 72 144.5 73 114.5 74 93.0 75 85.5 76 64.0 77 37.5 78 30.0 79 22.5 80 17.0 81 12.5 82 8.0 83 6.0 84 1.5 85 1.0 86 2.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.559392695604714 2 2.648567019034501 3 0.9971770007615276 4 0.9842697540800728 5 0.17812000420407462 6 0.0934853438213932 7 0.09311656534478022 8 0.10104530259195951 9 0.09606679315768415 10-11 1.880862425345407 12-13 1.4648803037259535 14-15 0.5508628494406553 16-17 0.0899819482935698 18-19 0.09800288015990234 20-21 0.12483151433349744 22-23 0.1423484919726145 24-25 0.15599329560729516 26-27 0.14917089378995485 28-29 0.597328937493892 30-31 0.18641751992786693 32-33 0.1656737306183862 34-35 0.14788016912180937 36-37 0.13773876101495214 38-39 0.44447025893780734 40-41 0.12372517890365847 42-43 0.12464712509519095 44-45 0.13156172153168452 46-47 0.1427172704492275 48-49 0.15617768484560168 50-51 0.1716663808633473 52-53 0.19204139169621504 54-55 0.18743166073855266 56-57 0.1589435234201991 58-59 0.14105776730446903 60-61 0.1181935017544636 62-63 0.12520029281011044 64-65 0.11699497170547138 66-67 0.11376816003510772 68-69 0.11173987841373625 70-71 0.12667540671656238 72-73 0.1404124049703963 74-75 0.1521211216028588 76-77 0.1732336893889525 78-79 0.17719805801254215 80-81 0.17563074948693694 82-83 0.16825517995467712 84-85 0.15534793327322244 86-87 0.15341184627100424 88-89 0.14990845074318082 90-91 0.15968108037342507 92-93 0.15175234312624578 94-95 0.2058705845692022 96-97 0.20494863837766972 98-99 0.2220968375401738 100-101 0.24735816318816367 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 542331.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.09761147643665 #Duplication Level Percentage of deduplicated Percentage of total 1 92.84361706993742 64.15272180365598 2 4.18316940105097 5.780940280278762 3 1.040274710857997 2.1564149339888505 4 0.4984107944809431 1.3775598173082528 5 0.3013348838609688 1.0410760364661191 6 0.20818044853311954 0.8630863049839085 7 0.13829287083209965 0.6688994941102222 8 0.10991096698510185 0.6075668234988818 9 0.0887167601366595 0.5517104601034007 >10 0.5208638193098689 6.689339706128505 >50 0.03572381152028106 1.703605447678004 >100 0.024190926558337773 3.4764045796060605 >500 0.003938057811822411 2.1135202615809137 >1k 0.0025316085933144074 4.706732265444493 >5k 8.438695311048026E-4 4.110421785167663 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9941 1.8330134180048716 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5942 1.0956408540171962 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5265 0.9708093396836988 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4549 0.8387866450562479 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4226 0.7792289210832499 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3771 0.6953318176537945 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3520 0.6490501188388641 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3088 0.569393967890458 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1461 0.2693926771657899 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1418 0.26146393991861056 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1098 0.20245938366053204 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1085 0.2000623235625476 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 997 0.183836070591576 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 968 0.17848878268068763 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 961 0.17719805801254215 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 960 0.17701366877423566 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 958 0.17664489029762268 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 918 0.16926932076536286 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 858 0.15820596646697313 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 765 0.14105776730446903 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 711 0.1311007484359183 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 626 0.11542766317986616 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 558 0.10288919497502447 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.219461915324773E-5 0.0 20-21 0.0 0.0 0.0 2.765838574597432E-4 0.0 22-23 0.0 0.0 0.0 0.0011063354298389727 0.0 24-25 0.0 0.0 0.0 0.0036877847661299095 0.0 26-27 0.0 0.0 0.0 0.006453623340727342 0.0 28-29 0.0 0.0 0.0 0.011063354298389729 0.0 30-31 0.0 0.0 0.0 0.018623313068956045 0.0 32-33 0.0 0.0 0.0 0.0326368951802497 0.0 34-35 0.0 0.0 0.0 0.05577774458771488 0.0 36-37 0.0 0.0 0.0 0.08583319043167364 0.0 38-39 0.0 0.0 0.0 0.12446273585688444 0.0 40-41 0.0 0.0 0.0 0.17268052167403303 0.0 42-43 0.0 0.0 0.0 0.22532364921053746 0.0 44-45 0.0 0.0 0.0 0.2827608969430108 0.0 46-47 0.0 0.0 0.0 0.34748151958859075 0.0 48-49 0.0 0.0 0.0 0.42317330191340713 0.0 50-51 0.0 0.0 0.0 0.4982197219041508 0.0 52-53 0.0 0.0 0.0 0.5717910279884425 0.0 54-55 0.0 0.0 0.0 0.6611276139479395 0.0 56-57 0.0 0.0 0.0 0.7752645524596602 0.0 58-59 0.0 0.0 0.0 0.899819482935698 0.0 60-61 0.0 0.0 0.0 1.0596849525474297 0.0 62-63 0.0 0.0 0.0 1.2149406912014986 0.0 64-65 0.0 0.0 0.0 1.3560906531251211 0.0 66-67 0.0 0.0 0.0 1.5095024993961252 0.0 68-69 0.0 0.0 0.0 1.6816298533552387 0.0 70-71 0.0 0.0 0.0 1.8877770217819008 0.0 72-73 0.0 0.0 0.0 2.0897754323466664 0.0 74-75 0.0 0.0 0.0 2.2984118554904662 0.0 76-77 0.0 0.0 0.0 2.504835607774588 0.0 78-79 0.0 0.0 0.0 2.736797269564159 0.0 80-81 0.0 0.0 0.0 2.974198413883772 0.0 82-83 0.0 0.0 0.0 3.2286555627467357 0.0 84-85 0.0 0.0 0.0 3.54386896563169 0.0 86-87 0.0 0.0 0.0 3.8696847497192675 0.0 88-89 0.0 0.0 0.0 4.164154363294741 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1395 0.0 69.91939 2 ACCTGGG 1455 0.0 67.53036 3 GTACCTG 1630 0.0 61.341938 1 GATCGTT 25 0.0046074726 58.247578 9 TCACGCA 50 4.104295E-7 57.264473 4 GTACCGG 45 1.0541784E-5 56.10963 1 GTACCAG 230 0.0 52.69426 1 CCTGGGA 1150 0.0 51.455032 4 CCTGGGG 545 0.0 50.785007 4 ATAACGC 110 0.0 48.165035 3 TATAACG 115 0.0 46.8828 2 ATCACGC 75 6.285889E-6 38.53203 3 TATCACG 80 8.681642E-6 36.76038 2 GTACCCG 55 0.0019093137 36.726303 1 TACCAGC 315 0.0 35.787884 2 CTGGGGA 380 0.0 33.255497 5 AGTACTC 325 0.0 32.901295 5 ACCAGCA 340 0.0 32.58223 3 ACACGGG 120 1.3305726E-7 32.110023 3 GGGATAG 230 0.0 31.656292 7 >>END_MODULE