FastQCFastQC Report
Fri 27 May 2016
SRR939540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542331
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123542.277944650038445No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91211.6818142425935452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77991.4380516695523582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66531.2267416024531144No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA62601.1542766317986617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58061.0705639176075128No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA54751.0095310797280628No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44300.8168443256977749No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT37640.694041092985649No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA23630.43571177011824885No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG19110.3523678344037129No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18280.3370635276242737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16480.30387346472910454No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16360.3016607938694266No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT14610.2693926771657899No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC14490.26718000630611194No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.2662580601145795No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13110.2417342914198156No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13090.24136551294320258No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG11640.21462907338876072No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG10560.1947150356516592No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10410.1919491970770618No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.18992091545569034No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT8730.16097180504157055No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8440.15562451713068218No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.14861772607503537No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT7630.14068898882785605No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA7250.13368219777220922No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG7190.13257586234237023No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6800.1253846820484169No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG6760.12464712509519095No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6680.12317201118873897No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6230.11487449546494669No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT5910.10897403983913884No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5740.1058394227879284No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA5450.10049213487704003No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.10030774563873354No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA5430.10012335640042704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13000.068.6089552
TATAACG1350.063.256492
ACCTGGG13750.062.4398733
ATCACGC850.061.3846663
TCACGCA900.057.990464
ATAACGC1550.055.0841873
TATCACG950.054.9332662
GTACCTG16300.054.739011
ACGGGAT352.8136236E-454.179845
CCTGGGA10700.047.0033154
TAACGCA1950.043.796994
GTACACG1155.4569682E-1141.2694631
ACACGGG1051.038643E-940.657383
CCTGGGG4750.039.955154
GTACCCG608.326289E-539.5499041
TACACGG1251.364242E-1037.9538882
GTACGTG958.746247E-734.9704441
CGGGATT550.002603231534.4780776
CTGGGAG3900.034.0360535
TACCCGG702.0665911E-433.88742
Produced by FastQC (version 0.11.5)