##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939540_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 542331 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.846538368634654 31.0 31.0 31.0 28.0 34.0 2 30.936376493322342 31.0 31.0 33.0 28.0 34.0 3 30.933345134244586 31.0 31.0 34.0 27.0 34.0 4 34.80686702401301 35.0 35.0 37.0 32.0 37.0 5 34.47822086511743 35.0 35.0 37.0 30.0 37.0 6 34.6097401771243 35.0 35.0 37.0 30.0 37.0 7 34.51185161829215 35.0 35.0 37.0 30.0 37.0 8 34.455432199155126 35.0 35.0 37.0 30.0 37.0 9 35.89506224058739 37.0 35.0 39.0 30.0 39.0 10-11 35.6558734426024 37.0 35.0 39.0 30.0 39.0 12-13 35.6374612552113 37.0 35.0 39.0 30.0 39.0 14-15 36.2058383164525 38.0 34.0 40.0 29.5 41.0 16-17 36.20108752772753 38.0 34.5 40.0 29.5 41.0 18-19 36.37923795615593 38.0 35.0 40.0 30.0 41.0 20-21 36.262073346351215 38.0 34.5 40.0 29.5 41.0 22-23 35.928082665383315 38.0 34.0 40.0 29.0 41.0 24-25 35.67396571466503 38.0 34.0 40.0 29.0 41.0 26-27 34.96872205350607 37.0 33.5 40.0 26.0 41.0 28-29 34.66048409550625 37.0 33.0 39.5 26.0 41.0 30-31 34.22341983032502 36.0 33.0 39.0 25.0 40.0 32-33 33.582870055372084 36.0 33.0 39.0 24.0 40.0 34-35 33.36600249663029 36.0 32.5 39.0 22.5 40.5 36-37 32.90829585622065 35.5 32.0 39.0 20.0 41.0 38-39 32.56887675607701 35.0 31.0 39.0 18.0 40.5 40-41 32.30869339941844 35.0 31.0 39.0 17.0 40.0 42-43 32.11263785400429 35.0 31.0 39.0 15.5 40.0 44-45 31.614851446810157 35.0 30.5 39.0 10.5 40.0 46-47 30.933074082064273 35.0 30.0 39.0 4.5 40.0 48-49 30.38644665342752 35.0 28.5 39.0 2.0 40.0 50-51 30.124678471265703 35.0 27.5 39.0 2.0 40.0 52-53 29.88758433502787 35.0 27.0 38.0 2.0 40.0 54-55 29.605471566257506 35.0 27.0 38.0 2.0 40.0 56-57 29.214120159091035 34.5 26.5 38.0 2.0 40.0 58-59 28.869530784705283 34.0 26.0 38.0 2.0 40.0 60-61 28.506091298487455 34.0 26.0 38.0 2.0 40.0 62-63 28.26510378348278 34.0 25.5 37.5 2.0 40.0 64-65 27.75628352426839 33.0 23.5 37.0 2.0 39.5 66-67 27.023174039470362 33.0 22.0 36.0 2.0 39.0 68-69 24.072058392383987 30.0 10.0 35.0 2.0 38.0 70-71 22.914086600249664 28.5 8.0 35.0 2.0 37.5 72-73 23.734714593117488 29.0 10.5 34.5 2.0 37.0 74-75 24.35779348774088 30.0 13.5 34.0 2.0 37.0 76-77 23.88350656702272 29.5 13.0 33.5 2.0 36.0 78-79 24.84810014548311 31.0 18.0 34.5 2.0 36.0 80-81 24.89125460281636 31.0 18.0 35.0 2.0 36.0 82-83 24.617875430318385 31.0 15.0 34.5 2.0 35.5 84-85 24.280315895643064 31.0 10.0 34.0 2.0 35.0 86-87 23.76284132752876 31.0 6.0 34.0 2.0 35.0 88-89 23.189832408621303 30.5 2.0 34.0 2.0 35.0 90-91 22.550572436390322 29.5 2.0 34.0 2.0 35.0 92-93 22.067726167229978 29.0 2.0 34.0 2.0 35.0 94-95 21.36772561406226 29.0 2.0 34.0 2.0 35.0 96-97 20.668758562575256 29.0 2.0 34.0 2.0 35.0 98-99 19.879646378318775 28.0 2.0 33.0 2.0 35.0 100-101 18.74399951321241 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.401563563584606 1101 2 0.40799171647805466 1101 3 0.3387798275203515 1101 4 0.36981300447239107 1101 5 0.7287947081082393 1101 6 0.3225051008525668 1101 7 0.5928222775221599 1101 8 0.8005476680748345 1101 9 0.5882122565408281 1101 10-11 0.8496330586925396 1101 12-13 0.928228681207294 1101 14-15 0.8901190050771461 1101 16-17 1.0268115972721077 1101 18-19 0.716908617817559 1101 20-21 1.019302558655319 1101 22-23 0.7213859889122816 1101 24-25 0.48718285373843173 1101 26-27 0.5100841364460464 1101 28-29 0.7056342160670042 1101 30-31 0.9103047364092802 1101 32-33 1.2305089373972535 1101 34-35 0.8899226920922985 1101 36-37 1.1679995049667724 1101 38-39 1.503414970856248 1101 40-41 1.3647038108553815 1101 42-43 1.2317092668926577 1101 44-45 1.1762884524525496 1101 46-47 1.4926437607877538 1101 48-49 1.5766940596970542 1101 50-51 1.3697094809776864 1101 52-53 1.1160695006532642 1101 54-55 1.4225336307432777 1101 56-57 1.5808672178774579 1101 58-59 1.5596128975256036 1101 60-61 1.565413001968615 1101 62-63 1.1896833923823813 1101 64-65 0.8350357817602649 1101 66-67 0.455412041844216 1101 68-69 -6.293672180837209 1101 70-71 -6.446170642724265 1101 72-73 -3.1895318572205156 1101 74-75 -1.2232641137334355 1101 76-77 0.7012980250536138 1101 78-79 0.9979649399922756 1101 80-81 1.0601787838888832 1101 82-83 0.5385937880666951 1101 84-85 0.4685115182532158 1101 86-87 0.34436102543116576 1101 88-89 -0.03784665165631296 1101 90-91 0.051250052840018157 1101 92-93 0.055729846556452856 1101 94-95 0.10915046049290922 1101 96-97 0.0172946527823008 1101 98-99 0.14812926721832653 1101 100-101 -0.29885894602149676 1102 1 0.4333279149288529 1102 2 0.43420980946283905 1102 3 0.4170237474746976 1102 4 0.3802803649022479 1102 5 0.699931723131229 1102 6 0.6411200109903561 1102 7 0.6285267164524058 1102 8 0.8252589741064753 1102 9 0.7699339690202294 1102 10-11 0.7191533233740941 1102 12-13 0.5444195879083225 1102 14-15 0.9454822048514515 1102 16-17 0.9960731434278784 1102 18-19 0.8286272526044556 1102 20-21 0.9187309413920346 1102 22-23 1.071830971952913 1102 24-25 1.0603357573489518 1102 26-27 1.24551639194582 1102 28-29 1.177375548836089 1102 30-31 0.9639030718905914 1102 32-33 1.519041006311916 1102 34-35 1.4244906289483126 1102 36-37 1.1017954539540185 1102 38-39 1.1314149195613723 1102 40-41 1.0810062839095451 1102 42-43 0.6158581887384997 1102 44-45 0.6634374833000045 1102 46-47 0.8632748801060437 1102 48-49 0.6628389933663676 1102 50-51 0.6677647640292186 1102 52-53 0.5016049870889745 1102 54-55 0.46017765713449066 1102 56-57 0.5822409587485744 1102 58-59 0.7467782786144674 1102 60-61 0.6834841864314001 1102 62-63 0.8320539209119495 1102 64-65 0.2955852139901154 1102 66-67 0.33029422392819896 1102 68-69 -6.16815843060478 1102 70-71 -7.148455188219614 1102 72-73 -0.7272560318539192 1102 74-75 0.9822279644143066 1102 76-77 1.586722906915103 1102 78-79 1.1468419028227252 1102 80-81 0.7973999481543075 1102 82-83 0.5813352426611083 1102 84-85 0.5057195382069217 1102 86-87 0.6572870133384008 1102 88-89 0.30004551369271937 1102 90-91 0.1898087309710874 1102 92-93 0.19056964624996908 1102 94-95 0.6441063148411104 1102 96-97 0.5304197289231247 1102 98-99 0.06760112236160154 1102 100-101 0.240604445467401 1103 1 0.4286692983516289 1103 2 0.21147513941712148 1103 3 0.33060330422285844 1103 4 0.3339147069813535 1103 5 0.2211222170688174 1103 6 0.4716426456554359 1103 7 0.2009091950848827 1103 8 0.3850010730927593 1103 9 0.2643995325264896 1103 10-11 0.5364610156817875 1103 12-13 0.475299693752099 1103 14-15 0.7704393455789358 1103 16-17 0.4655436761251579 1103 18-19 0.4367226859179141 1103 20-21 0.36487267514278443 1103 22-23 0.6522495641810977 1103 24-25 0.7086490275735571 1103 26-27 0.8710552385965826 1103 28-29 0.7380322895437033 1103 30-31 0.6582326037927899 1103 32-33 0.8454562940280752 1103 34-35 0.9946493946012609 1103 36-37 1.7944835999488546 1103 38-39 1.8001779636877941 1103 40-41 1.3029598592424847 1103 42-43 1.1549843564983888 1103 44-45 1.5906869739579186 1103 46-47 1.5816894597124822 1103 48-49 1.6935175363995647 1103 50-51 1.026015484561917 1103 52-53 0.9246772515493227 1103 54-55 0.8674541056536711 1103 56-57 1.3809064203864203 1103 58-59 1.33957481508833 1103 60-61 0.9422051166639562 1103 62-63 0.9709893959666118 1103 64-65 0.9479670093588304 1103 66-67 0.6317393267904521 1103 68-69 -6.0386038564644515 1103 70-71 -7.103092685735017 1103 72-73 -0.9405399217366437 1103 74-75 0.18760618196549217 1103 76-77 0.515786152293753 1103 78-79 0.10126355110338636 1103 80-81 0.24406655270608368 1103 82-83 -0.12622476032040097 1103 84-85 0.2313363390417429 1103 86-87 0.5304049322412006 1103 88-89 0.540054334006772 1103 90-91 0.23124557255327716 1103 92-93 0.4980181530080685 1103 94-95 0.5331535966936265 1103 96-97 0.48181638217297973 1103 98-99 0.4158824034190438 1103 100-101 0.6630866184946314 1104 1 0.30288715955754597 1104 2 0.20725040487029034 1104 3 0.19658500691531344 1104 4 0.04529956667039414 1104 5 0.5619628977665769 1104 6 0.1995709258981364 1104 7 0.2273715576180635 1104 8 0.5510278080188087 1104 9 0.5716734233114735 1104 10-11 0.31589787575004635 1104 12-13 0.4454984281093175 1104 14-15 0.31340365160851746 1104 16-17 0.3653469563678584 1104 18-19 0.4398097640369869 1104 20-21 0.6069357439709933 1104 22-23 0.7694489147688799 1104 24-25 1.0375058013695337 1104 26-27 1.0629606946072059 1104 28-29 0.7110870155581139 1104 30-31 0.44941932165399834 1104 32-33 0.510549795165737 1104 34-35 0.403672432143928 1104 36-37 0.5626653642643547 1104 38-39 0.5903692321747087 1104 40-41 0.6702777157081599 1104 42-43 0.6484309814509857 1104 44-45 0.6359414060777588 1104 46-47 0.29148041534388014 1104 48-49 0.33154133237984595 1104 50-51 0.25906321872952986 1104 52-53 0.12784669125353076 1104 54-55 0.042336192305171494 1104 56-57 0.3573207880390541 1104 58-59 0.17823498763288725 1104 60-61 0.3052597241724868 1104 62-63 0.12549143426770826 1104 64-65 -0.11117631164989206 1104 66-67 -0.023844077646408834 1104 68-69 -5.98302163708637 1104 70-71 -3.478862973941311 1104 72-73 -0.0486765353126799 1104 74-75 0.5819611544721255 1104 76-77 0.7647284873335742 1104 78-79 0.03588682091756823 1104 80-81 -0.0029856135813624007 1104 82-83 0.1699747561756162 1104 84-85 0.3189892116242241 1104 86-87 0.353606013014808 1104 88-89 0.09387377845121492 1104 90-91 0.11561938749449752 1104 92-93 -0.10465830224411121 1104 94-95 0.01807372373192706 1104 96-97 -0.04510519063703455 1104 98-99 0.20030243565264527 1104 100-101 0.026535258047154286 1105 1 0.23423664413544287 1105 2 0.28801435371631356 1105 3 0.04147743174596741 1105 4 0.23804541345182884 1105 5 0.6062706560425895 1105 6 0.5589699188114423 1105 7 0.35635745956328435 1105 8 0.8858145988426145 1105 9 0.4541809416945739 1105 10-11 0.48616390192966463 1105 12-13 0.44431055255231655 1105 14-15 0.45529538689189764 1105 16-17 0.3915540986869388 1105 18-19 0.7287460069894109 1105 20-21 0.6367803930261928 1105 22-23 0.7635950060788517 1105 24-25 0.5859378056343019 1105 26-27 0.6056793071685505 1105 28-29 0.6059832068234599 1105 30-31 0.49741094278543585 1105 32-33 0.5615313859918629 1105 34-35 0.5622958292362341 1105 36-37 0.7694426289056508 1105 38-39 0.5350127825898809 1105 40-41 0.6979899832756828 1105 42-43 0.6882740659871658 1105 44-45 0.3771467810697935 1105 46-47 0.33333419655458485 1105 48-49 0.20030281276251216 1105 50-51 0.39602527045137137 1105 52-53 0.23312288680682158 1105 54-55 0.11749684523110915 1105 56-57 0.39632656517042264 1105 58-59 0.34635124878008483 1105 60-61 0.8566788008744837 1105 62-63 0.47599729541954616 1105 64-65 0.428178585004936 1105 66-67 0.4950272087938501 1105 68-69 -2.1611971761211173 1105 70-71 -2.764318162060242 1105 72-73 1.0766443359505828 1105 74-75 1.5406955165250054 1105 76-77 1.3346219262043384 1105 78-79 0.7490496409920873 1105 80-81 0.43166529586775226 1105 82-83 0.610184054054244 1105 84-85 0.30854170123755864 1105 86-87 0.2852684013979676 1105 88-89 0.5017685152933176 1105 90-91 0.333225151877933 1105 92-93 -0.051844196077013294 1105 94-95 0.35575487475436773 1105 96-97 0.47305771635521054 1105 98-99 0.4384990087765246 1105 100-101 0.41822273667987986 1106 1 -0.1964397766919994 1106 2 -0.06872955808142223 1106 3 -0.031071361280339715 1106 4 0.07796102751655098 1106 5 0.3570388110187821 1106 6 0.06000252058272082 1106 7 0.1886018943286274 1106 8 0.37333004342434606 1106 9 0.18635633880921176 1106 10-11 0.22385194063816272 1106 12-13 -0.015469830680721941 1106 14-15 0.17060841189656628 1106 16-17 -0.14464029711509596 1106 18-19 -0.2843815171071 1106 20-21 -0.19499588942964152 1106 22-23 0.04683307930617531 1106 24-25 0.22084270849269672 1106 26-27 0.2685474215517871 1106 28-29 0.1856535446338725 1106 30-31 -0.34550321645153304 1106 32-33 0.22848694411533899 1106 34-35 0.5127013143220296 1106 36-37 0.38855628028625944 1106 38-39 0.6522449357645783 1106 40-41 0.3973324959498683 1106 42-43 0.2658954966961815 1106 44-45 0.43936807488870144 1106 46-47 0.7557186918241747 1106 48-49 0.8547486300586691 1106 50-51 0.884008867342601 1106 52-53 0.5667065927360575 1106 54-55 0.46903478482760974 1106 56-57 0.6411094817593117 1106 58-59 0.9320068249777975 1106 60-61 1.2164197763498201 1106 62-63 0.7861526449485758 1106 64-65 0.5474389043559214 1106 66-67 0.5826146540155825 1106 68-69 -2.405482310507498 1106 70-71 -2.5485369847460717 1106 72-73 0.521083842079527 1106 74-75 1.145934970505337 1106 76-77 1.1145399699202834 1106 78-79 0.9540440557572545 1106 80-81 0.6415041981394758 1106 82-83 0.869984881913453 1106 84-85 0.7424892622529136 1106 86-87 0.8851715116478367 1106 88-89 1.013158246165002 1106 90-91 0.7001410059447863 1106 92-93 0.3631150480051595 1106 94-95 0.13638403590246995 1106 96-97 0.4659050601834487 1106 98-99 0.36064498340741125 1106 100-101 0.1355224460106328 1107 1 -0.03932211668685426 1107 2 0.16514903422667615 1107 3 0.02917371077505848 1107 4 -0.11818651168847083 1107 5 0.16632216279607093 1107 6 0.07300008102153299 1107 7 0.15551127718474334 1107 8 -0.005725688304522691 1107 9 0.2665642749776467 1107 10-11 0.3173224721649106 1107 12-13 -0.04556601820764428 1107 14-15 0.07171783877017646 1107 16-17 -0.12134822243415044 1107 18-19 0.16584926476141248 1107 20-21 0.28555891700187175 1107 22-23 0.12634820489552112 1107 24-25 -0.017324489793722364 1107 26-27 0.5279050141047676 1107 28-29 0.2669433192456907 1107 30-31 0.19681867543281584 1107 32-33 0.3369869704638404 1107 34-35 -0.25818142245024944 1107 36-37 -0.2574717607182109 1107 38-39 -0.36024425360054835 1107 40-41 -0.1937945689201399 1107 42-43 -0.2830398688815201 1107 44-45 -0.048255879308307215 1107 46-47 -0.04569730760545454 1107 48-49 -0.1439540937563457 1107 50-51 -0.4117034566261637 1107 52-53 -0.8198600116241543 1107 54-55 -0.5193579256271619 1107 56-57 -0.30574094245155337 1107 58-59 -0.5698236208698297 1107 60-61 -0.787146570725028 1107 62-63 -1.009382989073849 1107 64-65 -1.6059310688926018 1107 66-67 -1.1728371607761545 1107 68-69 -3.3499582698254144 1107 70-71 -3.2141729600590736 1107 72-73 -0.7151785145923775 1107 74-75 -0.3301018429999729 1107 76-77 -0.4474363536269088 1107 78-79 -0.6457098182473153 1107 80-81 -0.6813253828573629 1107 82-83 -0.5875831824636855 1107 84-85 -0.6957932494721675 1107 86-87 -0.4538128001448243 1107 88-89 -0.3603890989910603 1107 90-91 -0.5024417670954264 1107 92-93 -0.20647754217645797 1107 94-95 -0.10452340646406455 1107 96-97 -0.007244267619800127 1107 98-99 -0.022521114441623524 1107 100-101 0.24252680746246114 1108 1 0.002783700209935347 1108 2 0.08306945803888155 1108 3 0.1415910123142261 1108 4 0.261137171170283 1108 5 0.07348018261275513 1108 6 0.07109701073480323 1108 7 0.33209144440737504 1108 8 0.5653872519545473 1108 9 0.04521399245680158 1108 10-11 0.5159906324065275 1108 12-13 0.33190719955928927 1108 14-15 0.4148027311770832 1108 16-17 0.5896604976505131 1108 18-19 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2316 96-97 0.6230049262124631 2316 98-99 0.6408689574559965 2316 100-101 0.13703111039541938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 38.0 5 170.0 6 511.0 7 1040.0 8 1624.0 9 2467.0 10 4099.0 11 6015.0 12 8017.0 13 8617.0 14 7912.0 15 9237.0 16 10723.0 17 11724.0 18 9551.0 19 7452.0 20 7052.0 21 7093.0 22 7448.0 23 8358.0 24 9645.0 25 11555.0 26 13846.0 27 16676.0 28 20262.0 29 23398.0 30 27486.0 31 31468.0 32 36070.0 33 41783.0 34 48591.0 35 50948.0 36 43857.0 37 33285.0 38 13477.0 39 820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.076569822449194 18.462738675534062 12.849056916956684 15.611634585060061 2 14.710232595882175 24.97012326917828 41.94582352810995 18.3738206068296 3 20.430813371367247 37.25768591780175 28.940317854857717 13.371182855973284 4 10.376063075832743 18.949880403968816 37.495385955587054 33.17867056461139 5 9.610367100534544 42.09237532060679 32.886558208916696 15.410699369941971 6 20.300148802115313 35.84600548373595 26.254077307032052 17.599768407116688 7 23.9361201922811 36.426647195163106 24.49279130272841 15.144441309827394 8 25.24509939501891 30.215864481285415 24.40539080377113 20.133645319924547 9 28.011306748092956 19.354969566556218 25.125799557834604 27.50792412751622 10-11 21.908865507515245 28.497439902483013 29.75964681442359 19.834047775578153 12-13 27.28933068550387 26.81176255829005 30.26601835410478 15.6328884021013 14-15 22.956644558396995 27.110565318965723 33.756598829865894 16.17619129277139 16-17 16.488775672957157 29.913898730746148 35.40533239662823 18.191993199668467 18-19 15.818121975437489 29.36041767888769 39.12042564451013 15.701034701164696 20-21 18.202629021759776 27.782756287212052 37.51730953974602 16.497305151282152 22-23 17.148214972235564 27.986440002913355 36.414234493542224 18.451110531308863 24-25 17.071081640254764 28.489174008769957 38.02650164298385 16.41324270799143 26-27 18.53781495560266 26.594611837877558 38.8386160531127 16.028957153407084 28-29 16.099342370324887 27.386454469770516 40.32948786386061 16.18471529604399 30-31 16.0966570169454 29.79532756808585 39.061641436026036 15.04637397894271 32-33 16.054384012641783 29.58483649669896 37.70085842805606 16.6599210626032 34-35 16.737335210452123 28.497908524571724 37.592622812525065 17.17213345245109 36-37 18.313759542741977 28.31406406152387 37.948761485202695 15.423414910531458 38-39 18.855693062225022 27.75959088253563 37.747749660130154 15.636966395109198 40-41 16.835355788102227 28.870707147134624 39.073992225137 15.219944839626148 42-43 16.24103318390947 30.739177535115225 36.71536322179755 16.304426059177757 44-45 16.341765447341274 31.347846387515528 35.21907071422903 17.091317450914172 46-47 16.703037930999482 30.14124353737228 34.101082279643194 19.05463625198504 48-49 17.565845624362765 30.47403313387803 34.07400914388001 17.88611209787919 50-51 18.613257894503743 31.1891027060543 34.04453991171737 16.15309948772458 52-53 16.74720291074407 31.520483697176342 34.7283723745 17.003941017579596 54-55 15.805791318096324 31.441667566054253 35.00121302018041 17.75132809566901 56-57 17.453545774553717 32.396179451780654 32.84695766207681 17.303317111588818 58-59 16.94050192167248 31.41630212674924 33.3594597186465 18.283736232931787 60-61 17.184393867306934 30.46116907688733 34.4573960765222 17.897040979283535 62-63 19.311423205607746 29.903848547402234 33.632909630173245 17.151818616816776 64-65 18.223265917271494 30.73451422080604 34.38929087744144 16.65292898448103 66-67 16.796594034759536 31.04296143913296 34.79138832232575 17.36905620378176 68-69 17.43434185872474 32.4404048957399 33.057664774404884 17.067588471130474 70-71 17.424496761323745 32.296460938262875 32.00370134429477 18.27534095611861 72-73 17.270773526307075 31.63713436956186 31.332020238558915 19.760071865572154 74-75 17.086930333422192 32.24188074947354 32.3652987074321 18.305890209672167 76-77 17.45053917792225 31.019865616576787 33.619503147128974 17.91009205837199 78-79 16.509529214656983 31.133870044176753 34.29853380383898 18.058066937327283 80-81 16.01395861280523 33.325404398714774 33.166917903257776 17.493719085222217 82-83 15.989150397179547 33.15029392023956 31.71757104609053 19.142984636490365 84-85 16.446905020928217 31.92198476942083 31.79300425939926 19.83810595025169 86-87 17.310227706469046 31.571523138510216 32.594625692163866 18.523623462856868 88-89 18.085239771430256 30.32836156650299 32.82592300983352 18.76047565223324 90-91 16.440581548762196 31.19421438456382 34.08462495194174 18.28057911473224 92-93 15.799688188311395 33.05664886334406 32.6086615792128 18.53500136913175 94-95 15.345879222520232 33.44746235910324 31.777414262529973 19.429244155846554 96-97 15.782168502249366 32.12902999706725 30.78125547585276 21.30754602483063 98-99 16.570927505015934 31.88307712734569 31.00012539832409 20.545869969314293 100-101 17.23428267941313 31.830386450979958 31.79544063083199 19.139890238774925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7483.0 1 5056.5 2 5753.5 3 7938.0 4 5708.0 5 4276.5 6 4558.5 7 6097.5 8 7073.5 9 6735.5 10 5671.0 11 4430.0 12 3939.0 13 3753.0 14 3649.0 15 3540.0 16 3357.0 17 3270.5 18 3352.0 19 3458.5 20 3554.5 21 3781.0 22 4057.0 23 4325.0 24 4616.0 25 5070.0 26 5781.5 27 6493.0 28 7090.5 29 7687.0 30 8400.0 31 9210.0 32 10324.5 33 11670.0 34 13058.0 35 14263.5 36 15220.5 37 15808.5 38 15284.5 39 13993.0 40 12892.5 41 12298.0 42 11939.0 43 12015.0 44 12872.0 45 13672.0 46 15168.0 47 19581.0 48 29339.5 49 28984.5 50 19825.5 51 14943.5 52 10510.5 53 8094.5 54 6927.0 55 6099.5 56 5392.0 57 4714.5 58 4209.5 59 3778.0 60 3350.0 61 2821.5 62 2152.5 63 1525.5 64 1027.0 65 705.0 66 519.0 67 397.5 68 307.0 69 236.0 70 193.0 71 160.5 72 141.5 73 130.5 74 112.5 75 82.5 76 56.0 77 41.5 78 23.5 79 16.5 80 10.0 81 7.0 82 6.0 83 2.5 84 1.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022126708596779458 2 0.018992091545569034 3 0.0014751139064519637 4 0.0934853438213932 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01226188434738195 12-13 0.0 14-15 0.0 16-17 8.297515723792297E-4 18-19 9.219461915324773E-5 20-21 0.0 22-23 9.219461915324773E-5 24-25 0.004609730957662386 26-27 0.002304865478831193 28-29 8.297515723792297E-4 30-31 1.8438923830649547E-4 32-33 4.609730957662387E-4 34-35 0.0019360870022182026 36-37 0.09081169986594902 38-39 0.10685356359861414 40-41 0.08417368728691518 42-43 0.08703172048066586 44-45 0.08740049895727886 46-47 0.08583319043167364 48-49 0.08085468099739827 50-51 0.08020931866332554 52-53 0.06250795178590197 54-55 0.05448701991956942 56-57 0.02904130503327304 58-59 0.01751697763911707 60-61 0.010510186583470242 62-63 0.001751697763911707 64-65 0.0049785094342753786 66-67 0.1494474776474146 68-69 0.7047356688074258 70-71 0.8645089438000041 72-73 0.34619079492044524 74-75 0.049877288961907025 76-77 0.19250236479198127 78-79 0.014290165968753401 80-81 0.0024892547171376893 82-83 0.0012907246681454685 84-85 1.8438923830649547E-4 86-87 0.0018438923830649547 88-89 3.6877847661299093E-4 90-91 0.0037799793852831575 92-93 0.003042422432057175 94-95 0.07384788994175144 96-97 0.032821284418556196 98-99 0.010878965060083233 100-101 0.011616522013309215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 542331.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.12215542649144 #Duplication Level Percentage of deduplicated Percentage of total 1 88.47018312451283 49.6513736792407 2 6.944125996727027 7.7943863697890805 3 1.5943779674486398 2.6843978429317827 4 0.744907602483552 1.6722328097982804 5 0.4459036064020686 1.2512535751864977 6 0.31055674835686975 1.045746846001802 7 0.22576475604815485 0.886928331013093 8 0.16435817475534484 0.7379308023387124 9 0.13450023191611662 0.6793598628445898 >10 0.8409453965128426 8.784511435169607 >50 0.0681742272102741 2.5724353010212306 >100 0.042567322225821956 4.683500782760822 >500 0.005986029688006213 2.1931863273381054 >1k 0.0053209152782277445 5.383580938819475 >5k 0.0019953432293354045 7.673445890747925 >10k+ 3.3255720488923403E-4 2.3057292049982943 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12354 2.277944650038445 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9121 1.6818142425935452 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7799 1.4380516695523582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6653 1.2267416024531144 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6260 1.1542766317986617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5806 1.0705639176075128 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5475 1.0095310797280628 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4430 0.8168443256977749 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3764 0.694041092985649 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2363 0.43571177011824885 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1911 0.3523678344037129 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1828 0.3370635276242737 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1648 0.30387346472910454 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1636 0.3016607938694266 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1461 0.2693926771657899 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1449 0.26718000630611194 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1444 0.2662580601145795 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1311 0.2417342914198156 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1309 0.24136551294320258 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1164 0.21462907338876072 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1056 0.1947150356516592 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1041 0.1919491970770618 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1030 0.18992091545569034 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 873 0.16097180504157055 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 844 0.15562451713068218 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 806 0.14861772607503537 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 763 0.14068898882785605 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 725 0.13368219777220922 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 719 0.13257586234237023 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 680 0.1253846820484169 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 676 0.12464712509519095 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 668 0.12317201118873897 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 623 0.11487449546494669 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 591 0.10897403983913884 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 574 0.1058394227879284 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 545 0.10049213487704003 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 544 0.10030774563873354 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 543 0.10012335640042704 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8438923830649547E-4 0.0 20-21 0.0 0.0 0.0 3.6877847661299093E-4 0.0 22-23 0.0 0.0 0.0 0.0013829192872987163 0.0 24-25 0.0 0.0 0.0 0.004609730957662387 0.0 26-27 0.0 0.0 0.0 0.00774434800887281 0.0 28-29 0.0 0.0 0.0 0.013183830538914428 0.0 30-31 0.0 0.0 0.0 0.02286426555000544 0.0 32-33 0.0 0.0 0.0 0.03982807547420303 0.0 34-35 0.0 0.0 0.0 0.06647232040949162 0.0 36-37 0.0 0.0 0.0 0.101967248783492 0.0 38-39 0.0 0.0 0.0 0.1471426121685834 0.0 40-41 0.0 0.0 0.0 0.19997012894339433 0.0 42-43 0.0 0.0 0.0 0.2575917659141742 0.0 44-45 0.0 0.0 0.0 0.3228655562746736 0.0 46-47 0.0 0.0 0.0 0.3962524731206588 0.0 48-49 0.0 0.0 0.0 0.4810715227416467 0.0 50-51 0.0 0.0 0.0 0.5643232638370295 0.0 52-53 0.0 0.0 0.0 0.6486813403622511 0.0 54-55 0.0 0.0 0.0 0.7478827505711456 0.0 56-57 0.0 0.0 0.0 0.8745581572877081 0.0 58-59 0.0 0.0 0.0 0.9942267729486236 0.0 60-61 0.0 9.219461915324773E-5 0.0 1.1412771904980539 0.0 62-63 0.0 1.8438923830649547E-4 0.0 1.2789237568938527 0.0 64-65 0.0 1.8438923830649547E-4 0.0 1.4092869483765451 0.0 66-67 0.0 1.8438923830649547E-4 0.0 1.5365155228080267 0.0 68-69 0.0 1.8438923830649547E-4 0.0 1.6785874309231814 0.0 70-71 0.0 1.8438923830649547E-4 0.0 1.8749619697195992 0.0 72-73 0.0 1.8438923830649547E-4 0.0 2.085073506769851 0.0 74-75 0.0 1.8438923830649547E-4 0.0 2.312978605316679 0.0 76-77 0.0 1.8438923830649547E-4 0.0 2.5489046357298406 0.0 78-79 0.0 1.8438923830649547E-4 0.0 2.8155314743210327 0.0 80-81 0.0 1.8438923830649547E-4 0.0 3.0929450833531553 0.0 82-83 0.0 1.8438923830649547E-4 0.0 3.3862161668796364 0.0 84-85 0.0 1.8438923830649547E-4 0.0 3.7582214551629907 0.0 86-87 0.0 1.8438923830649547E-4 0.0 4.138616453789291 0.0 88-89 0.0 1.8438923830649547E-4 0.0 4.4844384702331235 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1300 0.0 68.608955 2 TATAACG 135 0.0 63.25649 2 ACCTGGG 1375 0.0 62.439873 3 ATCACGC 85 0.0 61.384666 3 TCACGCA 90 0.0 57.99046 4 ATAACGC 155 0.0 55.084187 3 TATCACG 95 0.0 54.933266 2 GTACCTG 1630 0.0 54.73901 1 ACGGGAT 35 2.8136236E-4 54.17984 5 CCTGGGA 1070 0.0 47.003315 4 TAACGCA 195 0.0 43.79699 4 GTACACG 115 5.4569682E-11 41.269463 1 ACACGGG 105 1.038643E-9 40.65738 3 CCTGGGG 475 0.0 39.95515 4 GTACCCG 60 8.326289E-5 39.549904 1 TACACGG 125 1.364242E-10 37.953888 2 GTACGTG 95 8.746247E-7 34.970444 1 CGGGATT 55 0.0026032315 34.478077 6 CTGGGAG 390 0.0 34.036053 5 TACCCGG 70 2.0665911E-4 33.8874 2 >>END_MODULE