FastQCFastQC Report
Fri 27 May 2016
SRR939539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932060
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157401.6887324850331522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116191.2465935669377508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114821.2318949423856833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89770.9631354204664936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69870.7496298521554406No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53480.5737828036821664No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48900.5246443362015322No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA43080.4622020041628221No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.2584597558097118No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21380.22938437439649806No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.20245477758942557No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16100.17273566079436944No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15010.16104113469089973No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG12480.13389695942321309No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11060.11866188871961032No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT10780.11565779027101261No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10640.11415574104671374No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG9960.10686007338583353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10150.059.2600442
TATAACG859.640644E-1151.778152
GTACCTG12350.050.4654731
ACCTGGG12400.045.2372253
TATCACG1251.8189894E-1243.0333942
TACCCGG701.730513E-434.9297032
ATCACGC1651.8189894E-1234.8681643
ATAACGC1508.6401997E-1031.9624863
GTACCCG803.2260478E-431.4137421
ATGGGGA10700.028.477855
GTACATG108250.028.0445631
TACATGG107350.027.8331342
CATGGGG24450.027.8202634
TCGCGCT459.355706E-426.18905694-95
GTACTTG4250.026.0179711
ACATGGG110100.025.9096413
CATTCCG1908.1709E-925.8995599
TCACGCA2058.6765795E-1025.7034024
CCTGGGA12750.025.5476234
ATGGGAC10000.024.1835695
Produced by FastQC (version 0.11.5)